Human Labor Is Associated with Reduced Decidual Cell Expression of Progesterone, But Not Glucocorticoid, Receptors
Context: Unchanging plasma progesterone (P4) levels suggest that human labor is initiated by reduced P4 receptor (PR) expression, which elicits functional P4 withdrawal. The glucocorticoid receptor (GR) is also implicated in this process. Objective: Our objective was to compare PR and GR staining in...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2010-05, Vol.95 (5), p.2271-2275 |
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| container_title | The journal of clinical endocrinology and metabolism |
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| creator | Lockwood, C. J. Stocco, C. Murk, W. Kayisli, U. A. Funai, E. F. Schatz, F. |
| description | Context: Unchanging plasma progesterone (P4) levels suggest that human labor is initiated by reduced P4 receptor (PR) expression, which elicits functional P4 withdrawal. The glucocorticoid receptor (GR) is also implicated in this process.
Objective: Our objective was to compare PR and GR staining in human decidual cells (DCs) and interstitial trophoblasts (ITs) of gestational age-matched pre- and postcontraction specimens and to evaluate steroid effects on PR and GR expression in human DC cultures.
Interventions and Main Outcome Measures: Decidua basalis and parietalis sections were immunostained for PR or GR and then for the cytoplasmic DC and IT markers vimentin and cytokeratin. Western blotting measured PR and GR levels in nuclear extracts of cultured leukocyte-free term DCs after incubation with estradiol-17β (E2) with or without medroxyprogesterone acetate (MPA).
Results: PR histological scores (HSCOREs) were significantly higher in DC nuclei from pre- vs. post-uterine-contraction decidua basalis and parietalis sections with PR immunostaining absent from ITs. In contrast, immunoreactive GR was localized in IT and DC nuclei. GR HSCORES were significantly higher in ITs than DCs but similar in pre- vs. post-uterine-contraction specimens. In term DC monolayers, PR-A and PR-B were enhanced by E2 and inhibited by MPA, whereas E2 plus MPA produced intermediate PR expression. The GR was constitutively expressed.
Conclusions: In post- vs. pre-uterine-contraction specimens, significantly lower HSCOREs in DC nuclei, but not IT, and unchanging GR levels in DCs and ITs suggest that functional P4 withdrawal may occur in DCs and is unlikely to involve the GR. Nuclear extracts from DC monolayer cultures express steroid-regulated PR-A and PR-B and constitutive GR.
Progesterone expression in decidual cells is reduced in post-labor versus pre-labor placental sections and is steroid-regulated in monolayer cultures, while corresponding glucocorticoid receptor expression is unchanged. |
| doi_str_mv | 10.1210/jc.2009-2136 |
| format | Article |
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Objective: Our objective was to compare PR and GR staining in human decidual cells (DCs) and interstitial trophoblasts (ITs) of gestational age-matched pre- and postcontraction specimens and to evaluate steroid effects on PR and GR expression in human DC cultures.
Interventions and Main Outcome Measures: Decidua basalis and parietalis sections were immunostained for PR or GR and then for the cytoplasmic DC and IT markers vimentin and cytokeratin. Western blotting measured PR and GR levels in nuclear extracts of cultured leukocyte-free term DCs after incubation with estradiol-17β (E2) with or without medroxyprogesterone acetate (MPA).
Results: PR histological scores (HSCOREs) were significantly higher in DC nuclei from pre- vs. post-uterine-contraction decidua basalis and parietalis sections with PR immunostaining absent from ITs. In contrast, immunoreactive GR was localized in IT and DC nuclei. GR HSCORES were significantly higher in ITs than DCs but similar in pre- vs. post-uterine-contraction specimens. In term DC monolayers, PR-A and PR-B were enhanced by E2 and inhibited by MPA, whereas E2 plus MPA produced intermediate PR expression. The GR was constitutively expressed.
Conclusions: In post- vs. pre-uterine-contraction specimens, significantly lower HSCOREs in DC nuclei, but not IT, and unchanging GR levels in DCs and ITs suggest that functional P4 withdrawal may occur in DCs and is unlikely to involve the GR. Nuclear extracts from DC monolayer cultures express steroid-regulated PR-A and PR-B and constitutive GR.
Progesterone expression in decidual cells is reduced in post-labor versus pre-labor placental sections and is steroid-regulated in monolayer cultures, while corresponding glucocorticoid receptor expression is unchanged.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jc.2009-2136</identifier><identifier>PMID: 20237167</identifier><identifier>CODEN: JCEMAZ</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Biological and medical sciences ; Blotting, Western ; Brief Report ; Cytoplasm - metabolism ; Decidua - metabolism ; Delivery, Obstetric ; Endocrinopathies ; Feeding. Feeding behavior ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Keratins - metabolism ; Labor, Obstetric - metabolism ; Medical sciences ; Placenta - metabolism ; Pregnancy ; Progesterone - blood ; Receptors, Glucocorticoid - metabolism ; Receptors, Progesterone - metabolism ; Trophoblasts - metabolism ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Vertebrates: endocrinology ; Vimentin - metabolism</subject><ispartof>The journal of clinical endocrinology and metabolism, 2010-05, Vol.95 (5), p.2271-2275</ispartof><rights>Copyright © 2010 by The Endocrine Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 by The Endocrine Society 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5029-ed4d05890a834d6ecf8269c7d7edf24e3a9032fac0189c498ab343c5be41d8573</citedby><cites>FETCH-LOGICAL-c5029-ed4d05890a834d6ecf8269c7d7edf24e3a9032fac0189c498ab343c5be41d8573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,782,786,887,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22763407$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20237167$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lockwood, C. J.</creatorcontrib><creatorcontrib>Stocco, C.</creatorcontrib><creatorcontrib>Murk, W.</creatorcontrib><creatorcontrib>Kayisli, U. A.</creatorcontrib><creatorcontrib>Funai, E. F.</creatorcontrib><creatorcontrib>Schatz, F.</creatorcontrib><title>Human Labor Is Associated with Reduced Decidual Cell Expression of Progesterone, But Not Glucocorticoid, Receptors</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>Context: Unchanging plasma progesterone (P4) levels suggest that human labor is initiated by reduced P4 receptor (PR) expression, which elicits functional P4 withdrawal. The glucocorticoid receptor (GR) is also implicated in this process.
Objective: Our objective was to compare PR and GR staining in human decidual cells (DCs) and interstitial trophoblasts (ITs) of gestational age-matched pre- and postcontraction specimens and to evaluate steroid effects on PR and GR expression in human DC cultures.
Interventions and Main Outcome Measures: Decidua basalis and parietalis sections were immunostained for PR or GR and then for the cytoplasmic DC and IT markers vimentin and cytokeratin. Western blotting measured PR and GR levels in nuclear extracts of cultured leukocyte-free term DCs after incubation with estradiol-17β (E2) with or without medroxyprogesterone acetate (MPA).
Results: PR histological scores (HSCOREs) were significantly higher in DC nuclei from pre- vs. post-uterine-contraction decidua basalis and parietalis sections with PR immunostaining absent from ITs. In contrast, immunoreactive GR was localized in IT and DC nuclei. GR HSCORES were significantly higher in ITs than DCs but similar in pre- vs. post-uterine-contraction specimens. In term DC monolayers, PR-A and PR-B were enhanced by E2 and inhibited by MPA, whereas E2 plus MPA produced intermediate PR expression. The GR was constitutively expressed.
Conclusions: In post- vs. pre-uterine-contraction specimens, significantly lower HSCOREs in DC nuclei, but not IT, and unchanging GR levels in DCs and ITs suggest that functional P4 withdrawal may occur in DCs and is unlikely to involve the GR. Nuclear extracts from DC monolayer cultures express steroid-regulated PR-A and PR-B and constitutive GR.
Progesterone expression in decidual cells is reduced in post-labor versus pre-labor placental sections and is steroid-regulated in monolayer cultures, while corresponding glucocorticoid receptor expression is unchanged.</description><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Brief Report</subject><subject>Cytoplasm - metabolism</subject><subject>Decidua - metabolism</subject><subject>Delivery, Obstetric</subject><subject>Endocrinopathies</subject><subject>Feeding. Feeding behavior</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Keratins - metabolism</subject><subject>Labor, Obstetric - metabolism</subject><subject>Medical sciences</subject><subject>Placenta - metabolism</subject><subject>Pregnancy</subject><subject>Progesterone - blood</subject><subject>Receptors, Glucocorticoid - metabolism</subject><subject>Receptors, Progesterone - metabolism</subject><subject>Trophoblasts - metabolism</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Vertebrates: endocrinology</subject><subject>Vimentin - metabolism</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU1vEzEQhlcIREPhxhn5grhkW3_ten1BKqFfUgQIgcTNcsazjcNmndq7BP49ThNakLA0skZ-5vXMvEXxktETxhk9XcEJp1SXnIn6UTFhWlalYlo9LiaUclZqxb8dFc9SWlHKpKzE0-KIUy4Uq9WkiFfj2vZkbhchkutEzlIK4O2Ajmz9sCSf0Y2Qk_cI3o22IzPsOnL-cxMxJR96ElryKYYbTAPG0OOUvBsH8iEM5LIbIUCIg4fg3TQrAW6GENPz4klru4QvDvdx8fXi_Mvsqpx_vLyenc1LqCjXJTrpaNVoahshXY3QNrzWoJxC13KJwmoqeGuBskaD1I1dCCmgWqBkrqmUOC7e7nU342KNDrAfou3MJvq1jb9MsN78-9L7pbkJPwxval3JJgu8OQjEcDvmCc3aJ8jz2x7DmIwSgtFKizqT0z0JMaQUsb3_hVGzc8mswOxcMjuXMv7q787u4T-2ZOD1AbAJbNdG24NPDxxXtZB0x8k9tw1d3n_63o1bjGaJthuWhuYja9WUnDJKq5yVOYTOZWJfhr0LEH2Pd3aaVRhjny35f9e_ASlYvKc</recordid><startdate>201005</startdate><enddate>201005</enddate><creator>Lockwood, C. J.</creator><creator>Stocco, C.</creator><creator>Murk, W.</creator><creator>Kayisli, U. A.</creator><creator>Funai, E. F.</creator><creator>Schatz, F.</creator><general>Endocrine Society</general><general>Copyright by The Endocrine Society</general><general>The Endocrine Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201005</creationdate><title>Human Labor Is Associated with Reduced Decidual Cell Expression of Progesterone, But Not Glucocorticoid, Receptors</title><author>Lockwood, C. J. ; Stocco, C. ; Murk, W. ; Kayisli, U. A. ; Funai, E. F. ; Schatz, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5029-ed4d05890a834d6ecf8269c7d7edf24e3a9032fac0189c498ab343c5be41d8573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Brief Report</topic><topic>Cytoplasm - metabolism</topic><topic>Decidua - metabolism</topic><topic>Delivery, Obstetric</topic><topic>Endocrinopathies</topic><topic>Feeding. Feeding behavior</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Keratins - metabolism</topic><topic>Labor, Obstetric - metabolism</topic><topic>Medical sciences</topic><topic>Placenta - metabolism</topic><topic>Pregnancy</topic><topic>Progesterone - blood</topic><topic>Receptors, Glucocorticoid - metabolism</topic><topic>Receptors, Progesterone - metabolism</topic><topic>Trophoblasts - metabolism</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Vertebrates: endocrinology</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lockwood, C. J.</creatorcontrib><creatorcontrib>Stocco, C.</creatorcontrib><creatorcontrib>Murk, W.</creatorcontrib><creatorcontrib>Kayisli, U. A.</creatorcontrib><creatorcontrib>Funai, E. F.</creatorcontrib><creatorcontrib>Schatz, F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lockwood, C. J.</au><au>Stocco, C.</au><au>Murk, W.</au><au>Kayisli, U. A.</au><au>Funai, E. F.</au><au>Schatz, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Labor Is Associated with Reduced Decidual Cell Expression of Progesterone, But Not Glucocorticoid, Receptors</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>2010-05</date><risdate>2010</risdate><volume>95</volume><issue>5</issue><spage>2271</spage><epage>2275</epage><pages>2271-2275</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><coden>JCEMAZ</coden><abstract>Context: Unchanging plasma progesterone (P4) levels suggest that human labor is initiated by reduced P4 receptor (PR) expression, which elicits functional P4 withdrawal. The glucocorticoid receptor (GR) is also implicated in this process.
Objective: Our objective was to compare PR and GR staining in human decidual cells (DCs) and interstitial trophoblasts (ITs) of gestational age-matched pre- and postcontraction specimens and to evaluate steroid effects on PR and GR expression in human DC cultures.
Interventions and Main Outcome Measures: Decidua basalis and parietalis sections were immunostained for PR or GR and then for the cytoplasmic DC and IT markers vimentin and cytokeratin. Western blotting measured PR and GR levels in nuclear extracts of cultured leukocyte-free term DCs after incubation with estradiol-17β (E2) with or without medroxyprogesterone acetate (MPA).
Results: PR histological scores (HSCOREs) were significantly higher in DC nuclei from pre- vs. post-uterine-contraction decidua basalis and parietalis sections with PR immunostaining absent from ITs. In contrast, immunoreactive GR was localized in IT and DC nuclei. GR HSCORES were significantly higher in ITs than DCs but similar in pre- vs. post-uterine-contraction specimens. In term DC monolayers, PR-A and PR-B were enhanced by E2 and inhibited by MPA, whereas E2 plus MPA produced intermediate PR expression. The GR was constitutively expressed.
Conclusions: In post- vs. pre-uterine-contraction specimens, significantly lower HSCOREs in DC nuclei, but not IT, and unchanging GR levels in DCs and ITs suggest that functional P4 withdrawal may occur in DCs and is unlikely to involve the GR. Nuclear extracts from DC monolayer cultures express steroid-regulated PR-A and PR-B and constitutive GR.
Progesterone expression in decidual cells is reduced in post-labor versus pre-labor placental sections and is steroid-regulated in monolayer cultures, while corresponding glucocorticoid receptor expression is unchanged.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>20237167</pmid><doi>10.1210/jc.2009-2136</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Biological and medical sciences Blotting, Western Brief Report Cytoplasm - metabolism Decidua - metabolism Delivery, Obstetric Endocrinopathies Feeding. Feeding behavior Female Fundamental and applied biological sciences. Psychology Humans Keratins - metabolism Labor, Obstetric - metabolism Medical sciences Placenta - metabolism Pregnancy Progesterone - blood Receptors, Glucocorticoid - metabolism Receptors, Progesterone - metabolism Trophoblasts - metabolism Vertebrates: anatomy and physiology, studies on body, several organs or systems Vertebrates: endocrinology Vimentin - metabolism |
| title | Human Labor Is Associated with Reduced Decidual Cell Expression of Progesterone, But Not Glucocorticoid, Receptors |
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