Landscape phage ligands for PC3 prostate carcinoma cells
Tumor-specific cytotoxicity of drugs can be enhanced by targeting them to tumor receptors using tumor-specific ligands. Phage display technology with its high throughput capacity for the analysis of targeting ligands possessing specific binding properties represents a very attractive tool in the que...
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| Veröffentlicht in: | Protein engineering, design and selection design and selection, 2010-06, Vol.23 (6), p.423-430 |
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| container_title | Protein engineering, design and selection |
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| creator | Jayanna, P.K. Bedi, D. Deinnocentes, P. Bird, R.C. Petrenko, V.A. |
| description | Tumor-specific cytotoxicity of drugs can be enhanced by targeting them to tumor receptors using tumor-specific ligands. Phage display technology with its high throughput capacity for the analysis of targeting ligands possessing specific binding properties represents a very attractive tool in the quest for molecular ligands. Also, current phage nanobiotechnology concepts allow the use of intact phage particles and isolated phage coat proteins per se as components of nanomedicines. Herein, we describe the use of two landscape phage libraries to obtain phage ligands against PC3 prostate carcinoma cells. Following a very stringent selection scheme, we were able to identify three phage ligands, bearing the fusion peptides, DTDSHVNL, DTPYDLTG and DVVYALSDD that demonstrated specificity and selectivity to PC3 cells based on target-association assays, microscopy and flow cytometry. The phage ligands and their fusion coat proteins can be used as navigating modules in both therapeutic and diagnostic approaches to prostate carcinoma. |
| doi_str_mv | 10.1093/protein/gzq011 |
| format | Article |
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Phage display technology with its high throughput capacity for the analysis of targeting ligands possessing specific binding properties represents a very attractive tool in the quest for molecular ligands. Also, current phage nanobiotechnology concepts allow the use of intact phage particles and isolated phage coat proteins per se as components of nanomedicines. Herein, we describe the use of two landscape phage libraries to obtain phage ligands against PC3 prostate carcinoma cells. Following a very stringent selection scheme, we were able to identify three phage ligands, bearing the fusion peptides, DTDSHVNL, DTPYDLTG and DVVYALSDD that demonstrated specificity and selectivity to PC3 cells based on target-association assays, microscopy and flow cytometry. 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All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org 2010</rights><rights>The Author 2010. Published by Oxford University Press. All rights reserved. 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The phage ligands and their fusion coat proteins can be used as navigating modules in both therapeutic and diagnostic approaches to prostate carcinoma.</description><subject>affinity selection</subject><subject>Analysis of Variance</subject><subject>Cell Line, Tumor</subject><subject>Escherichia coli</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>landscape phage</subject><subject>Ligands</subject><subject>Male</subject><subject>Oligopeptides - chemistry</subject><subject>Oligopeptides - metabolism</subject><subject>Original</subject><subject>PC3 cells</subject><subject>Peptide Library</subject><subject>phage display</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Recombinant Fusion Proteins - chemistry</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Sensitivity and Specificity</subject><issn>1741-0126</issn><issn>1741-0134</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTFPwzAQhS0EoqWwMqJsiCGtL-fEyYKECrSISjCAhFgsx3XaQJqkdoKAX0-ilAqmTj7Zn9-9u0fIKdAh0AhHpSkqneajxfeaAuyRPnAGLgVk-9vaC3rkyNo3Sr2AAxySnkch9H0P-yScyXxulSy1Uy7lQjtZumhvnKQwzuMYnaaBrWSlHSWNSvNiJR2ls8wek4NEZlafbM4Beb69eRpP3dnD5G58NXMVi7BymQoZBD6gjBKY86ZSYUwxShhgEs-1Rg9VHHnIuVahFygeKhZyqQExkRHHAbnsdMs6Xum50nllZCZKk66k-RKFTMX_lzxdikXxIbww8NGPGoHzjYAp1rW2lVilth1B5rqoreA-85ExTneTiNgsPWhNDTtSNduxRidbP0BFm4vY5CK6XJoPZ3-n2OK_QTTARQcUdblbzO3Y1Fb6c0tL8y4ab9wX05dXMQF2fR9NZ2KCP9SGqMg</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>Jayanna, P.K.</creator><creator>Bedi, D.</creator><creator>Deinnocentes, P.</creator><creator>Bird, R.C.</creator><creator>Petrenko, V.A.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20100601</creationdate><title>Landscape phage ligands for PC3 prostate carcinoma cells</title><author>Jayanna, P.K. ; Bedi, D. ; Deinnocentes, P. ; Bird, R.C. ; Petrenko, V.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-4c8416513a9f1d7651c8b039f413fbdee323cb92377ec826c78c487ae133fa973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>affinity selection</topic><topic>Analysis of Variance</topic><topic>Cell Line, Tumor</topic><topic>Escherichia coli</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>landscape phage</topic><topic>Ligands</topic><topic>Male</topic><topic>Oligopeptides - chemistry</topic><topic>Oligopeptides - metabolism</topic><topic>Original</topic><topic>PC3 cells</topic><topic>Peptide Library</topic><topic>phage display</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Recombinant Fusion Proteins - chemistry</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jayanna, P.K.</creatorcontrib><creatorcontrib>Bedi, D.</creatorcontrib><creatorcontrib>Deinnocentes, P.</creatorcontrib><creatorcontrib>Bird, R.C.</creatorcontrib><creatorcontrib>Petrenko, V.A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Protein engineering, design and selection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jayanna, P.K.</au><au>Bedi, D.</au><au>Deinnocentes, P.</au><au>Bird, R.C.</au><au>Petrenko, V.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Landscape phage ligands for PC3 prostate carcinoma cells</atitle><jtitle>Protein engineering, design and selection</jtitle><addtitle>Protein Eng Des Sel</addtitle><date>2010-06-01</date><risdate>2010</risdate><volume>23</volume><issue>6</issue><spage>423</spage><epage>430</epage><pages>423-430</pages><issn>1741-0126</issn><eissn>1741-0134</eissn><abstract>Tumor-specific cytotoxicity of drugs can be enhanced by targeting them to tumor receptors using tumor-specific ligands. 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| ispartof | Protein engineering, design and selection, 2010-06, Vol.23 (6), p.423-430 |
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| source | MEDLINE; Oxford University Press Journals Current; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | affinity selection Analysis of Variance Cell Line, Tumor Escherichia coli Flow Cytometry Humans landscape phage Ligands Male Oligopeptides - chemistry Oligopeptides - metabolism Original PC3 cells Peptide Library phage display Prostatic Neoplasms - metabolism Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - metabolism Sensitivity and Specificity |
| title | Landscape phage ligands for PC3 prostate carcinoma cells |
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