Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf
Myricetin inhibited UVB-induced wrinkle formation and MMP-9 expression by directly inhibiting Raf kinase activity in mouse skin. Chronic exposure to solar ultraviolet (UV) light causes skin photoaging. Many studies have shown that naturally occurring phytochemicals have anti-photoaging effects, but...
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| Veröffentlicht in: | Biochemical pharmacology 2010-05, Vol.79 (10), p.1455-1461 |
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| creator | Jung, Sung Keun Lee, Ki Won Kim, Ho Young Oh, Mi Hyun Byun, Sanguine Lim, Sung Hwan Heo, Yong-Seok Kang, Nam Joo Bode, Ann M. Dong, Zigang Lee, Hyong Joo |
| description | Myricetin inhibited UVB-induced wrinkle formation and MMP-9 expression by directly inhibiting Raf kinase activity in mouse skin.
Chronic exposure to solar ultraviolet (UV) light causes skin photoaging. Many studies have shown that naturally occurring phytochemicals have anti-photoaging effects, but their direct target molecule(s) and mechanism(s) remain unclear. We found that myricetin, a major flavonoid in berries and red wine, inhibited wrinkle formation in mouse skin induced by chronic UVB irradiation (0.18
J/cm
2, 3 days/week for 15 weeks). Myricetin treatment reduced UVB-induced epidermal thickening of mouse skin and also suppressed UVB-induced matrix metalloproteinase-9 (MMP-9) protein expression and enzyme activity. Myricetin appeared to exert its anti-aging effects by suppressing UVB-induced Raf kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK and ERK in mouse skin.
In vitro and
in vivo pull-down assays revealed that myricetin bound with Raf in an ATP-noncompetitive manner. Overall, these results indicate that myricetin exerts potent anti-photoaging activity by regulating MMP-9 expression through the suppression of Raf kinase activity. |
| doi_str_mv | 10.1016/j.bcp.2010.01.004 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2864126</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006295210000158</els_id><sourcerecordid>20093107</sourcerecordid><originalsourceid>FETCH-LOGICAL-c480t-3ecef7faaac9afd1223a8b9d3a58e8ae964688a3430b9987f62d1f223c9d4a223</originalsourceid><addsrcrecordid>eNp9kM1OGzEUhS1EBYH2Adggb7qc1PZMHFtIlQripxIRqCqVurLu2NfgkHhG9oQ2b1-nobTdsLr--c6x9RFyxNmYMy4_zMet7ceClT3jY8aaHTLialpXQku1S0aMMVnWE7FPDnKeb7ZK8j2yLxjTNWfTEfk-W6dgcQiR5lXfJ8wZM737dlqF6FYWHf2RQnxcIPVdWsIQukghOjqb3Vaa4s_fic1hu6YhPoQ2lKp7-gX8W_LGwyLju-d5SO4uzr-eXVXXN5efzz5dV7ZRbKhqtOinHgCsBu-4EDWoVrsaJgoVoJaNVArqpmat1mrqpXDcF8pq10CZh-TjtrdftUt0FuOQYGH6FJaQ1qaDYP6_ieHB3HdPRijZcCFLAd8W2NTlnNC_ZDkzG89mbopns_FsGDfFc8kc__voS-KP2AK8fwYgW1j4BNGG_JcTE8kbrgp3suWwKHoKmEy2AWMRHxLawbguvPKNX55OnQY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Jung, Sung Keun ; Lee, Ki Won ; Kim, Ho Young ; Oh, Mi Hyun ; Byun, Sanguine ; Lim, Sung Hwan ; Heo, Yong-Seok ; Kang, Nam Joo ; Bode, Ann M. ; Dong, Zigang ; Lee, Hyong Joo</creator><creatorcontrib>Jung, Sung Keun ; Lee, Ki Won ; Kim, Ho Young ; Oh, Mi Hyun ; Byun, Sanguine ; Lim, Sung Hwan ; Heo, Yong-Seok ; Kang, Nam Joo ; Bode, Ann M. ; Dong, Zigang ; Lee, Hyong Joo</creatorcontrib><description>Myricetin inhibited UVB-induced wrinkle formation and MMP-9 expression by directly inhibiting Raf kinase activity in mouse skin.
Chronic exposure to solar ultraviolet (UV) light causes skin photoaging. Many studies have shown that naturally occurring phytochemicals have anti-photoaging effects, but their direct target molecule(s) and mechanism(s) remain unclear. We found that myricetin, a major flavonoid in berries and red wine, inhibited wrinkle formation in mouse skin induced by chronic UVB irradiation (0.18
J/cm
2, 3 days/week for 15 weeks). Myricetin treatment reduced UVB-induced epidermal thickening of mouse skin and also suppressed UVB-induced matrix metalloproteinase-9 (MMP-9) protein expression and enzyme activity. Myricetin appeared to exert its anti-aging effects by suppressing UVB-induced Raf kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK and ERK in mouse skin.
In vitro and
in vivo pull-down assays revealed that myricetin bound with Raf in an ATP-noncompetitive manner. Overall, these results indicate that myricetin exerts potent anti-photoaging activity by regulating MMP-9 expression through the suppression of Raf kinase activity.</description><identifier>ISSN: 0006-2952</identifier><identifier>EISSN: 1873-2968</identifier><identifier>DOI: 10.1016/j.bcp.2010.01.004</identifier><identifier>PMID: 20093107</identifier><identifier>CODEN: BCPCA6</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Anti-wrinkle ; Biological and medical sciences ; Blotting, Western ; Dermatology ; Epidermis - drug effects ; Epidermis - enzymology ; Epidermis - radiation effects ; Female ; Flavonoids - pharmacology ; Gene Expression - drug effects ; Immunoprecipitation ; Matrix Metalloproteinase 9 - biosynthesis ; Matrix Metalloproteinase Inhibitors ; Medical sciences ; Mice ; Mice, Hairless ; Mice, Inbred ICR ; Myricetin ; Pharmacology. Drug treatments ; Photoaging ; Raf ; raf Kinases - antagonists & inhibitors ; raf Kinases - physiology ; Skin Aging - drug effects ; Skin Aging - radiation effects ; Skin involvement in other diseases. Miscellaneous. General aspects ; Ultraviolet Rays - adverse effects ; UVB</subject><ispartof>Biochemical pharmacology, 2010-05, Vol.79 (10), p.1455-1461</ispartof><rights>2010 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>2010 Elsevier Inc. All rights reserved.</rights><rights>2010 Elsevier Inc. All rights reserved. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-3ecef7faaac9afd1223a8b9d3a58e8ae964688a3430b9987f62d1f223c9d4a223</citedby><cites>FETCH-LOGICAL-c480t-3ecef7faaac9afd1223a8b9d3a58e8ae964688a3430b9987f62d1f223c9d4a223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bcp.2010.01.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22561418$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20093107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jung, Sung Keun</creatorcontrib><creatorcontrib>Lee, Ki Won</creatorcontrib><creatorcontrib>Kim, Ho Young</creatorcontrib><creatorcontrib>Oh, Mi Hyun</creatorcontrib><creatorcontrib>Byun, Sanguine</creatorcontrib><creatorcontrib>Lim, Sung Hwan</creatorcontrib><creatorcontrib>Heo, Yong-Seok</creatorcontrib><creatorcontrib>Kang, Nam Joo</creatorcontrib><creatorcontrib>Bode, Ann M.</creatorcontrib><creatorcontrib>Dong, Zigang</creatorcontrib><creatorcontrib>Lee, Hyong Joo</creatorcontrib><title>Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf</title><title>Biochemical pharmacology</title><addtitle>Biochem Pharmacol</addtitle><description>Myricetin inhibited UVB-induced wrinkle formation and MMP-9 expression by directly inhibiting Raf kinase activity in mouse skin.
Chronic exposure to solar ultraviolet (UV) light causes skin photoaging. Many studies have shown that naturally occurring phytochemicals have anti-photoaging effects, but their direct target molecule(s) and mechanism(s) remain unclear. We found that myricetin, a major flavonoid in berries and red wine, inhibited wrinkle formation in mouse skin induced by chronic UVB irradiation (0.18
J/cm
2, 3 days/week for 15 weeks). Myricetin treatment reduced UVB-induced epidermal thickening of mouse skin and also suppressed UVB-induced matrix metalloproteinase-9 (MMP-9) protein expression and enzyme activity. Myricetin appeared to exert its anti-aging effects by suppressing UVB-induced Raf kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK and ERK in mouse skin.
In vitro and
in vivo pull-down assays revealed that myricetin bound with Raf in an ATP-noncompetitive manner. Overall, these results indicate that myricetin exerts potent anti-photoaging activity by regulating MMP-9 expression through the suppression of Raf kinase activity.</description><subject>Animals</subject><subject>Anti-wrinkle</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Dermatology</subject><subject>Epidermis - drug effects</subject><subject>Epidermis - enzymology</subject><subject>Epidermis - radiation effects</subject><subject>Female</subject><subject>Flavonoids - pharmacology</subject><subject>Gene Expression - drug effects</subject><subject>Immunoprecipitation</subject><subject>Matrix Metalloproteinase 9 - biosynthesis</subject><subject>Matrix Metalloproteinase Inhibitors</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Hairless</subject><subject>Mice, Inbred ICR</subject><subject>Myricetin</subject><subject>Pharmacology. Drug treatments</subject><subject>Photoaging</subject><subject>Raf</subject><subject>raf Kinases - antagonists & inhibitors</subject><subject>raf Kinases - physiology</subject><subject>Skin Aging - drug effects</subject><subject>Skin Aging - radiation effects</subject><subject>Skin involvement in other diseases. Miscellaneous. General aspects</subject><subject>Ultraviolet Rays - adverse effects</subject><subject>UVB</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1OGzEUhS1EBYH2Adggb7qc1PZMHFtIlQripxIRqCqVurLu2NfgkHhG9oQ2b1-nobTdsLr--c6x9RFyxNmYMy4_zMet7ceClT3jY8aaHTLialpXQku1S0aMMVnWE7FPDnKeb7ZK8j2yLxjTNWfTEfk-W6dgcQiR5lXfJ8wZM737dlqF6FYWHf2RQnxcIPVdWsIQukghOjqb3Vaa4s_fic1hu6YhPoQ2lKp7-gX8W_LGwyLju-d5SO4uzr-eXVXXN5efzz5dV7ZRbKhqtOinHgCsBu-4EDWoVrsaJgoVoJaNVArqpmat1mrqpXDcF8pq10CZh-TjtrdftUt0FuOQYGH6FJaQ1qaDYP6_ieHB3HdPRijZcCFLAd8W2NTlnNC_ZDkzG89mbopns_FsGDfFc8kc__voS-KP2AK8fwYgW1j4BNGG_JcTE8kbrgp3suWwKHoKmEy2AWMRHxLawbguvPKNX55OnQY</recordid><startdate>20100515</startdate><enddate>20100515</enddate><creator>Jung, Sung Keun</creator><creator>Lee, Ki Won</creator><creator>Kim, Ho Young</creator><creator>Oh, Mi Hyun</creator><creator>Byun, Sanguine</creator><creator>Lim, Sung Hwan</creator><creator>Heo, Yong-Seok</creator><creator>Kang, Nam Joo</creator><creator>Bode, Ann M.</creator><creator>Dong, Zigang</creator><creator>Lee, Hyong Joo</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20100515</creationdate><title>Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf</title><author>Jung, Sung Keun ; Lee, Ki Won ; Kim, Ho Young ; Oh, Mi Hyun ; Byun, Sanguine ; Lim, Sung Hwan ; Heo, Yong-Seok ; Kang, Nam Joo ; Bode, Ann M. ; Dong, Zigang ; Lee, Hyong Joo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-3ecef7faaac9afd1223a8b9d3a58e8ae964688a3430b9987f62d1f223c9d4a223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Anti-wrinkle</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Dermatology</topic><topic>Epidermis - drug effects</topic><topic>Epidermis - enzymology</topic><topic>Epidermis - radiation effects</topic><topic>Female</topic><topic>Flavonoids - pharmacology</topic><topic>Gene Expression - drug effects</topic><topic>Immunoprecipitation</topic><topic>Matrix Metalloproteinase 9 - biosynthesis</topic><topic>Matrix Metalloproteinase Inhibitors</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Hairless</topic><topic>Mice, Inbred ICR</topic><topic>Myricetin</topic><topic>Pharmacology. Drug treatments</topic><topic>Photoaging</topic><topic>Raf</topic><topic>raf Kinases - antagonists & inhibitors</topic><topic>raf Kinases - physiology</topic><topic>Skin Aging - drug effects</topic><topic>Skin Aging - radiation effects</topic><topic>Skin involvement in other diseases. Miscellaneous. General aspects</topic><topic>Ultraviolet Rays - adverse effects</topic><topic>UVB</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jung, Sung Keun</creatorcontrib><creatorcontrib>Lee, Ki Won</creatorcontrib><creatorcontrib>Kim, Ho Young</creatorcontrib><creatorcontrib>Oh, Mi Hyun</creatorcontrib><creatorcontrib>Byun, Sanguine</creatorcontrib><creatorcontrib>Lim, Sung Hwan</creatorcontrib><creatorcontrib>Heo, Yong-Seok</creatorcontrib><creatorcontrib>Kang, Nam Joo</creatorcontrib><creatorcontrib>Bode, Ann M.</creatorcontrib><creatorcontrib>Dong, Zigang</creatorcontrib><creatorcontrib>Lee, Hyong Joo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jung, Sung Keun</au><au>Lee, Ki Won</au><au>Kim, Ho Young</au><au>Oh, Mi Hyun</au><au>Byun, Sanguine</au><au>Lim, Sung Hwan</au><au>Heo, Yong-Seok</au><au>Kang, Nam Joo</au><au>Bode, Ann M.</au><au>Dong, Zigang</au><au>Lee, Hyong Joo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>2010-05-15</date><risdate>2010</risdate><volume>79</volume><issue>10</issue><spage>1455</spage><epage>1461</epage><pages>1455-1461</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><coden>BCPCA6</coden><abstract>Myricetin inhibited UVB-induced wrinkle formation and MMP-9 expression by directly inhibiting Raf kinase activity in mouse skin.
Chronic exposure to solar ultraviolet (UV) light causes skin photoaging. Many studies have shown that naturally occurring phytochemicals have anti-photoaging effects, but their direct target molecule(s) and mechanism(s) remain unclear. We found that myricetin, a major flavonoid in berries and red wine, inhibited wrinkle formation in mouse skin induced by chronic UVB irradiation (0.18
J/cm
2, 3 days/week for 15 weeks). Myricetin treatment reduced UVB-induced epidermal thickening of mouse skin and also suppressed UVB-induced matrix metalloproteinase-9 (MMP-9) protein expression and enzyme activity. Myricetin appeared to exert its anti-aging effects by suppressing UVB-induced Raf kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK and ERK in mouse skin.
In vitro and
in vivo pull-down assays revealed that myricetin bound with Raf in an ATP-noncompetitive manner. Overall, these results indicate that myricetin exerts potent anti-photoaging activity by regulating MMP-9 expression through the suppression of Raf kinase activity.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>20093107</pmid><doi>10.1016/j.bcp.2010.01.004</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Anti-wrinkle Biological and medical sciences Blotting, Western Dermatology Epidermis - drug effects Epidermis - enzymology Epidermis - radiation effects Female Flavonoids - pharmacology Gene Expression - drug effects Immunoprecipitation Matrix Metalloproteinase 9 - biosynthesis Matrix Metalloproteinase Inhibitors Medical sciences Mice Mice, Hairless Mice, Inbred ICR Myricetin Pharmacology. Drug treatments Photoaging Raf raf Kinases - antagonists & inhibitors raf Kinases - physiology Skin Aging - drug effects Skin Aging - radiation effects Skin involvement in other diseases. Miscellaneous. General aspects Ultraviolet Rays - adverse effects UVB |
| title | Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf |
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