Quantitative imaging of collective cell migration during Drosophila gastrulation: multiphoton microscopy and computational analysis

This protocol describes imaging and computational tools to collect and analyze live imaging data of embryonic cell migration. Our five-step protocol requires a few weeks to move through embryo preparation and four-dimensional (4D) live imaging using multi-photon microscopy, to 3D cell tracking using...

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Veröffentlicht in:	Nature protocols 2009-10, Vol.4 (10), p.1397-1412
Hauptverfasser:	Supatto, Willy, Stathopoulos, Angelike, McMahon, Amy, Fraser, Scott E
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Animals Automation Biological Techniques Cell adhesion & migration Cell migration Cell Movement Cell Survival Computational biology Computational Biology/Bioinformatics Cytological Techniques Data analysis Datasets Decomposition Development Biology Drosophila Drosophila melanogaster - cytology Drosophila melanogaster - embryology Drosophila melanogaster - metabolism Drosophila Proteins - genetics Drosophila Proteins - metabolism Embryonic development Embryos Experimental data Gastrulation Genetic aspects Image Processing, Computer-Assisted - methods Insects Life Sciences Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Methods Microarrays Microscope and microscopy Microscopy Microscopy, Fluorescence, Multiphoton - methods Organic Chemistry Physiological aspects protocol Registration Zebrafish - embryology Zebrafish - genetics Zebrafish - metabolism
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