Monitoring the Effects of Chiral Pharmaceuticals on Aquatic Microorganisms by Metabolic Fingerprinting
The effects of the chiral pharmaceuticals atenolol and propranolol on Pseudomonas putida, Pseudomonas aeruginosa, Micrococcus luteus, and Blastomonas natatoria were investigated. The growth dynamics of exposed cultures were monitored using a Bioscreen instrument. In addition, Fourier-transform infra...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2010-04, Vol.76 (7), p.2075-2085 |
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| creator | Wharfe, Emma S Winder, Catherine L Jarvis, Roger M Goodacre, Royston |
| description | The effects of the chiral pharmaceuticals atenolol and propranolol on Pseudomonas putida, Pseudomonas aeruginosa, Micrococcus luteus, and Blastomonas natatoria were investigated. The growth dynamics of exposed cultures were monitored using a Bioscreen instrument. In addition, Fourier-transform infrared (FT-IR) spectroscopy with appropriate chemometrics and high-performance liquid chromatography (HPLC) were employed in order to investigate the phenotypic changes and possible degradation of the drugs in exposed cultures. For the majority of the bacteria studied there was not a statistically significant difference in the organism's phenotype when it was exposed to the different enantiomers or mixtures of enantiomers. In contrast, the pseudomonads appeared to respond differently to propranolol, and the two enantiomers had different effects on the cellular phenotype. This implies that there were different metabolic responses in the organisms when they were exposed to the different enantiomers. We suggest that our findings may indicate that there are widespread effects on aquatic communities in which active pharmaceutical ingredients are present. |
| doi_str_mv | 10.1128/AEM.02395-09 |
| format | Article |
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The growth dynamics of exposed cultures were monitored using a Bioscreen instrument. In addition, Fourier-transform infrared (FT-IR) spectroscopy with appropriate chemometrics and high-performance liquid chromatography (HPLC) were employed in order to investigate the phenotypic changes and possible degradation of the drugs in exposed cultures. For the majority of the bacteria studied there was not a statistically significant difference in the organism's phenotype when it was exposed to the different enantiomers or mixtures of enantiomers. In contrast, the pseudomonads appeared to respond differently to propranolol, and the two enantiomers had different effects on the cellular phenotype. This implies that there were different metabolic responses in the organisms when they were exposed to the different enantiomers. We suggest that our findings may indicate that there are widespread effects on aquatic communities in which active pharmaceutical ingredients are present.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AEM.02395-09</identifier><identifier>PMID: 20118361</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Antimetabolites - metabolism ; Antimetabolites - pharmacology ; Aquatic communities ; Aquatic life ; Atenolol - metabolism ; Atenolol - pharmacology ; Bacteria ; Biological and medical sciences ; Blastomonas natatoria ; Chromatography, High Pressure Liquid ; DNA fingerprints ; Fourier transforms ; Fundamental and applied biological sciences. Psychology ; Metabolism ; Metabolome - drug effects ; Microbiology ; Micrococcus luteus - chemistry ; Micrococcus luteus - drug effects ; Micrococcus luteus - growth & development ; Pharmaceuticals ; Physiology ; Propranolol - metabolism ; Propranolol - pharmacology ; Pseudomonas - chemistry ; Pseudomonas - drug effects ; Pseudomonas - growth & development ; Spectroscopy, Fourier Transform Infrared ; Sphingomonadaceae - chemistry ; Sphingomonadaceae - drug effects ; Sphingomonadaceae - growth & development</subject><ispartof>Applied and Environmental Microbiology, 2010-04, Vol.76 (7), p.2075-2085</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Apr 2010</rights><rights>Copyright © 2010, American Society for Microbiology 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-10c4ed9edcdcc8622ea72619bb74f5569d71751e9909df37a5934b2b72be4ad3</citedby><cites>FETCH-LOGICAL-c522t-10c4ed9edcdcc8622ea72619bb74f5569d71751e9909df37a5934b2b72be4ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2849255/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2849255/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3174,3175,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22582128$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20118361$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wharfe, Emma S</creatorcontrib><creatorcontrib>Winder, Catherine L</creatorcontrib><creatorcontrib>Jarvis, Roger M</creatorcontrib><creatorcontrib>Goodacre, Royston</creatorcontrib><title>Monitoring the Effects of Chiral Pharmaceuticals on Aquatic Microorganisms by Metabolic Fingerprinting</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The effects of the chiral pharmaceuticals atenolol and propranolol on Pseudomonas putida, Pseudomonas aeruginosa, Micrococcus luteus, and Blastomonas natatoria were investigated. The growth dynamics of exposed cultures were monitored using a Bioscreen instrument. In addition, Fourier-transform infrared (FT-IR) spectroscopy with appropriate chemometrics and high-performance liquid chromatography (HPLC) were employed in order to investigate the phenotypic changes and possible degradation of the drugs in exposed cultures. For the majority of the bacteria studied there was not a statistically significant difference in the organism's phenotype when it was exposed to the different enantiomers or mixtures of enantiomers. In contrast, the pseudomonads appeared to respond differently to propranolol, and the two enantiomers had different effects on the cellular phenotype. This implies that there were different metabolic responses in the organisms when they were exposed to the different enantiomers. We suggest that our findings may indicate that there are widespread effects on aquatic communities in which active pharmaceutical ingredients are present.</description><subject>Antimetabolites - metabolism</subject><subject>Antimetabolites - pharmacology</subject><subject>Aquatic communities</subject><subject>Aquatic life</subject><subject>Atenolol - metabolism</subject><subject>Atenolol - pharmacology</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Blastomonas natatoria</subject><subject>Chromatography, High Pressure Liquid</subject><subject>DNA fingerprints</subject><subject>Fourier transforms</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metabolism</subject><subject>Metabolome - drug effects</subject><subject>Microbiology</subject><subject>Micrococcus luteus - chemistry</subject><subject>Micrococcus luteus - drug effects</subject><subject>Micrococcus luteus - growth & development</subject><subject>Pharmaceuticals</subject><subject>Physiology</subject><subject>Propranolol - metabolism</subject><subject>Propranolol - pharmacology</subject><subject>Pseudomonas - chemistry</subject><subject>Pseudomonas - drug effects</subject><subject>Pseudomonas - growth & development</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Sphingomonadaceae - chemistry</subject><subject>Sphingomonadaceae - drug effects</subject><subject>Sphingomonadaceae - growth & development</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1v1DAQhiMEokvhxhlSJMSFlLEdx_EFabXaAlJXIFHOluPYiaskbu0E1H_PLLuUj5NtzePHM36z7DmBc0Jo_W693Z0DZZIXIB9kKwKyLjhj1cNsBSBlQWkJJ9mTlK4BoISqfpydUCCkZhVZZW4XJj-H6Kcun3ubb52zZk55cPmm91EP-Zdex1Ebu8ze6AErU76-XTSe8p03MYTY6cmnMeXNXb6zs27CgLULNNp4g-IZd0-zRw4v22fH9TS7uthebT4Wl58_fNqsLwvDKZ0LAqa0rbStaY2pK0qtFrQismlE6TivZCuI4MRKCbJ1TGguWdnQRtDGlrplp9n7g_ZmaUa02GnGERR2Mep4p4L26t_K5HvVhe-K1qWknKPgzVEQw-1i06xGn4wdBj3ZsCQlSl4Br-mefPUfeR2WOOFwigKXFStrgdDbA4T_lFK07r4VAmqfnsL01K_0FEjEX_zd_j38Oy4EXh8BnTAMF_VkfPrDUWwNrcidHbjed_0PH63SaVTajkpUSqBP7Ad4eWCcDkp3ET3fvuJLDEhNAahkPwEWWLff</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Wharfe, Emma S</creator><creator>Winder, Catherine L</creator><creator>Jarvis, Roger M</creator><creator>Goodacre, Royston</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>5PM</scope></search><sort><creationdate>20100401</creationdate><title>Monitoring the Effects of Chiral Pharmaceuticals on Aquatic Microorganisms by Metabolic Fingerprinting</title><author>Wharfe, Emma S ; Winder, Catherine L ; Jarvis, Roger M ; Goodacre, Royston</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-10c4ed9edcdcc8622ea72619bb74f5569d71751e9909df37a5934b2b72be4ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Antimetabolites - metabolism</topic><topic>Antimetabolites - pharmacology</topic><topic>Aquatic communities</topic><topic>Aquatic life</topic><topic>Atenolol - metabolism</topic><topic>Atenolol - pharmacology</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Blastomonas natatoria</topic><topic>Chromatography, High Pressure Liquid</topic><topic>DNA fingerprints</topic><topic>Fourier transforms</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Metabolism</topic><topic>Metabolome - drug effects</topic><topic>Microbiology</topic><topic>Micrococcus luteus - chemistry</topic><topic>Micrococcus luteus - drug effects</topic><topic>Micrococcus luteus - growth & development</topic><topic>Pharmaceuticals</topic><topic>Physiology</topic><topic>Propranolol - metabolism</topic><topic>Propranolol - pharmacology</topic><topic>Pseudomonas - chemistry</topic><topic>Pseudomonas - drug effects</topic><topic>Pseudomonas - growth & development</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Sphingomonadaceae - chemistry</topic><topic>Sphingomonadaceae - drug effects</topic><topic>Sphingomonadaceae - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wharfe, Emma S</creatorcontrib><creatorcontrib>Winder, Catherine L</creatorcontrib><creatorcontrib>Jarvis, Roger M</creatorcontrib><creatorcontrib>Goodacre, Royston</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wharfe, Emma S</au><au>Winder, Catherine L</au><au>Jarvis, Roger M</au><au>Goodacre, Royston</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring the Effects of Chiral Pharmaceuticals on Aquatic Microorganisms by Metabolic Fingerprinting</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2010-04-01</date><risdate>2010</risdate><volume>76</volume><issue>7</issue><spage>2075</spage><epage>2085</epage><pages>2075-2085</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><eissn>1098-6596</eissn><coden>AEMIDF</coden><abstract>The effects of the chiral pharmaceuticals atenolol and propranolol on Pseudomonas putida, Pseudomonas aeruginosa, Micrococcus luteus, and Blastomonas natatoria were investigated. The growth dynamics of exposed cultures were monitored using a Bioscreen instrument. In addition, Fourier-transform infrared (FT-IR) spectroscopy with appropriate chemometrics and high-performance liquid chromatography (HPLC) were employed in order to investigate the phenotypic changes and possible degradation of the drugs in exposed cultures. For the majority of the bacteria studied there was not a statistically significant difference in the organism's phenotype when it was exposed to the different enantiomers or mixtures of enantiomers. In contrast, the pseudomonads appeared to respond differently to propranolol, and the two enantiomers had different effects on the cellular phenotype. This implies that there were different metabolic responses in the organisms when they were exposed to the different enantiomers. We suggest that our findings may indicate that there are widespread effects on aquatic communities in which active pharmaceutical ingredients are present.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>20118361</pmid><doi>10.1128/AEM.02395-09</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 2010-04, Vol.76 (7), p.2075-2085 |
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| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Antimetabolites - metabolism Antimetabolites - pharmacology Aquatic communities Aquatic life Atenolol - metabolism Atenolol - pharmacology Bacteria Biological and medical sciences Blastomonas natatoria Chromatography, High Pressure Liquid DNA fingerprints Fourier transforms Fundamental and applied biological sciences. Psychology Metabolism Metabolome - drug effects Microbiology Micrococcus luteus - chemistry Micrococcus luteus - drug effects Micrococcus luteus - growth & development Pharmaceuticals Physiology Propranolol - metabolism Propranolol - pharmacology Pseudomonas - chemistry Pseudomonas - drug effects Pseudomonas - growth & development Spectroscopy, Fourier Transform Infrared Sphingomonadaceae - chemistry Sphingomonadaceae - drug effects Sphingomonadaceae - growth & development |
| title | Monitoring the Effects of Chiral Pharmaceuticals on Aquatic Microorganisms by Metabolic Fingerprinting |
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