Myeloid dendritic cells from human squamous cell carcinoma are poor stimulators of T cell proliferation

In order to determine the phenotype and function of myeloid DCs from human cutaneous squamous cell carcinoma (SCC), we studied their surface marker expression and allo-stimulatory potential ex vivo . There were abundant CD11c + myeloid DCs, as well as TNF and inducible nitric oxide synthase (iNOS)-p...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of investigative dermatology 2009-04, Vol.129 (10), p.2451-2462
Hauptverfasser: Bluth, Mark J., Zaba, Lisa C., Moussai, Dariush, Suárez-Fariñas, Mayte, Kaporis, Helen, Fan, Linda, Pierson, Katherine C., White, Traci R., Pitts-Kiefer, Alexander, Fuentes-Duculan, Judilyn, Guttman-Yassky, Emma, Krueger, James G., Lowes, Michelle A., Carucci, John A.
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 2462
container_issue 10
container_start_page 2451
container_title Journal of investigative dermatology
container_volume 129
creator Bluth, Mark J.
Zaba, Lisa C.
Moussai, Dariush
Suárez-Fariñas, Mayte
Kaporis, Helen
Fan, Linda
Pierson, Katherine C.
White, Traci R.
Pitts-Kiefer, Alexander
Fuentes-Duculan, Judilyn
Guttman-Yassky, Emma
Krueger, James G.
Lowes, Michelle A.
Carucci, John A.
description In order to determine the phenotype and function of myeloid DCs from human cutaneous squamous cell carcinoma (SCC), we studied their surface marker expression and allo-stimulatory potential ex vivo . There were abundant CD11c + myeloid DCs, as well as TNF and inducible nitric oxide synthase (iNOS)-producing DCs (TIP-DCs), in and around SCC tumor nests. Although myeloid DCs from SCC, adjacent non-tumor bearing skin, and normal skin, were phenotypically similar by flow cytometry, and there was a pronounced genomic signature of mature DCs in SCC, they showed different T cell stimulatory potential in an allo-MLR. Myeloid DCs from SCC were less potent stimulators of allogeneic T cell proliferation than DCs from non-tumor bearing skin. Culture with a DC-maturing cytokine cocktail (IL-1β, IL-6, TNF, and PGE 2 ) enhanced stimulatory potential in DCs from non-tumor-bearing skin, while SCC associated DCs remained poor stimulators of T cell proliferation. The microenvironment associated with SCC showed expression of TGFβ, IL-10 and VEGF-A, factors capable of suppressing DC function. These findings indicate that CD11c + /HLA-DR hi DCs from SCC are mature, but are not potent stimulators of T cell proliferation compared with phenotypically similar DCs isolated from non-tumor-bearing skin. Identification of mechanisms responsible for suppression of tumor-associated DCs may provide insight into evasion of immunosurveillance by SCC.
doi_str_mv 10.1038/jid.2009.96
format Article
fullrecord <record><control><sourceid>pubmedcentral</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2846605</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>pubmedcentral_primary_oai_pubmedcentral_nih_gov_2846605</sourcerecordid><originalsourceid>FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_28466053</originalsourceid><addsrcrecordid>eNqljLtOxDAQAC0E4sKj4gf2B3LYzruhQSAauivooiVx7vZke8M6Qbq_RwIaaqopZjRK3Rm9Nbpo7480bq3W3barz1RmKlvkpimbc5VpbW1utX3bqKuUjlqbuqzaS7UxXdE2ZWsytX89Oc80wujiKLTQAIPzPsEkHOCwBoyQPlYMvKZvAwPKQJEDAoqDmVkgLRRWjwtLAp5g9xPOwp4mJ7gQxxt1MaFP7vaX1-rh-Wn3-JLP63tw4-DiIuj7WSignHpG6v-aSId-z5-9bcu61lXx78EX8ERm8A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Myeloid dendritic cells from human squamous cell carcinoma are poor stimulators of T cell proliferation</title><source>EZB-FREE-00999 freely available EZB journals</source><source>ProQuest Central UK/Ireland</source><source>Alma/SFX Local Collection</source><creator>Bluth, Mark J. ; Zaba, Lisa C. ; Moussai, Dariush ; Suárez-Fariñas, Mayte ; Kaporis, Helen ; Fan, Linda ; Pierson, Katherine C. ; White, Traci R. ; Pitts-Kiefer, Alexander ; Fuentes-Duculan, Judilyn ; Guttman-Yassky, Emma ; Krueger, James G. ; Lowes, Michelle A. ; Carucci, John A.</creator><creatorcontrib>Bluth, Mark J. ; Zaba, Lisa C. ; Moussai, Dariush ; Suárez-Fariñas, Mayte ; Kaporis, Helen ; Fan, Linda ; Pierson, Katherine C. ; White, Traci R. ; Pitts-Kiefer, Alexander ; Fuentes-Duculan, Judilyn ; Guttman-Yassky, Emma ; Krueger, James G. ; Lowes, Michelle A. ; Carucci, John A.</creatorcontrib><description>In order to determine the phenotype and function of myeloid DCs from human cutaneous squamous cell carcinoma (SCC), we studied their surface marker expression and allo-stimulatory potential ex vivo . There were abundant CD11c + myeloid DCs, as well as TNF and inducible nitric oxide synthase (iNOS)-producing DCs (TIP-DCs), in and around SCC tumor nests. Although myeloid DCs from SCC, adjacent non-tumor bearing skin, and normal skin, were phenotypically similar by flow cytometry, and there was a pronounced genomic signature of mature DCs in SCC, they showed different T cell stimulatory potential in an allo-MLR. Myeloid DCs from SCC were less potent stimulators of allogeneic T cell proliferation than DCs from non-tumor bearing skin. Culture with a DC-maturing cytokine cocktail (IL-1β, IL-6, TNF, and PGE 2 ) enhanced stimulatory potential in DCs from non-tumor-bearing skin, while SCC associated DCs remained poor stimulators of T cell proliferation. The microenvironment associated with SCC showed expression of TGFβ, IL-10 and VEGF-A, factors capable of suppressing DC function. These findings indicate that CD11c + /HLA-DR hi DCs from SCC are mature, but are not potent stimulators of T cell proliferation compared with phenotypically similar DCs isolated from non-tumor-bearing skin. Identification of mechanisms responsible for suppression of tumor-associated DCs may provide insight into evasion of immunosurveillance by SCC.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1038/jid.2009.96</identifier><identifier>PMID: 19387481</identifier><language>eng</language><ispartof>Journal of investigative dermatology, 2009-04, Vol.129 (10), p.2451-2462</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids></links><search><creatorcontrib>Bluth, Mark J.</creatorcontrib><creatorcontrib>Zaba, Lisa C.</creatorcontrib><creatorcontrib>Moussai, Dariush</creatorcontrib><creatorcontrib>Suárez-Fariñas, Mayte</creatorcontrib><creatorcontrib>Kaporis, Helen</creatorcontrib><creatorcontrib>Fan, Linda</creatorcontrib><creatorcontrib>Pierson, Katherine C.</creatorcontrib><creatorcontrib>White, Traci R.</creatorcontrib><creatorcontrib>Pitts-Kiefer, Alexander</creatorcontrib><creatorcontrib>Fuentes-Duculan, Judilyn</creatorcontrib><creatorcontrib>Guttman-Yassky, Emma</creatorcontrib><creatorcontrib>Krueger, James G.</creatorcontrib><creatorcontrib>Lowes, Michelle A.</creatorcontrib><creatorcontrib>Carucci, John A.</creatorcontrib><title>Myeloid dendritic cells from human squamous cell carcinoma are poor stimulators of T cell proliferation</title><title>Journal of investigative dermatology</title><description>In order to determine the phenotype and function of myeloid DCs from human cutaneous squamous cell carcinoma (SCC), we studied their surface marker expression and allo-stimulatory potential ex vivo . There were abundant CD11c + myeloid DCs, as well as TNF and inducible nitric oxide synthase (iNOS)-producing DCs (TIP-DCs), in and around SCC tumor nests. Although myeloid DCs from SCC, adjacent non-tumor bearing skin, and normal skin, were phenotypically similar by flow cytometry, and there was a pronounced genomic signature of mature DCs in SCC, they showed different T cell stimulatory potential in an allo-MLR. Myeloid DCs from SCC were less potent stimulators of allogeneic T cell proliferation than DCs from non-tumor bearing skin. Culture with a DC-maturing cytokine cocktail (IL-1β, IL-6, TNF, and PGE 2 ) enhanced stimulatory potential in DCs from non-tumor-bearing skin, while SCC associated DCs remained poor stimulators of T cell proliferation. The microenvironment associated with SCC showed expression of TGFβ, IL-10 and VEGF-A, factors capable of suppressing DC function. These findings indicate that CD11c + /HLA-DR hi DCs from SCC are mature, but are not potent stimulators of T cell proliferation compared with phenotypically similar DCs isolated from non-tumor-bearing skin. Identification of mechanisms responsible for suppression of tumor-associated DCs may provide insight into evasion of immunosurveillance by SCC.</description><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqljLtOxDAQAC0E4sKj4gf2B3LYzruhQSAauivooiVx7vZke8M6Qbq_RwIaaqopZjRK3Rm9Nbpo7480bq3W3barz1RmKlvkpimbc5VpbW1utX3bqKuUjlqbuqzaS7UxXdE2ZWsytX89Oc80wujiKLTQAIPzPsEkHOCwBoyQPlYMvKZvAwPKQJEDAoqDmVkgLRRWjwtLAp5g9xPOwp4mJ7gQxxt1MaFP7vaX1-rh-Wn3-JLP63tw4-DiIuj7WSignHpG6v-aSId-z5-9bcu61lXx78EX8ERm8A</recordid><startdate>20090423</startdate><enddate>20090423</enddate><creator>Bluth, Mark J.</creator><creator>Zaba, Lisa C.</creator><creator>Moussai, Dariush</creator><creator>Suárez-Fariñas, Mayte</creator><creator>Kaporis, Helen</creator><creator>Fan, Linda</creator><creator>Pierson, Katherine C.</creator><creator>White, Traci R.</creator><creator>Pitts-Kiefer, Alexander</creator><creator>Fuentes-Duculan, Judilyn</creator><creator>Guttman-Yassky, Emma</creator><creator>Krueger, James G.</creator><creator>Lowes, Michelle A.</creator><creator>Carucci, John A.</creator><scope>5PM</scope></search><sort><creationdate>20090423</creationdate><title>Myeloid dendritic cells from human squamous cell carcinoma are poor stimulators of T cell proliferation</title><author>Bluth, Mark J. ; Zaba, Lisa C. ; Moussai, Dariush ; Suárez-Fariñas, Mayte ; Kaporis, Helen ; Fan, Linda ; Pierson, Katherine C. ; White, Traci R. ; Pitts-Kiefer, Alexander ; Fuentes-Duculan, Judilyn ; Guttman-Yassky, Emma ; Krueger, James G. ; Lowes, Michelle A. ; Carucci, John A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_28466053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bluth, Mark J.</creatorcontrib><creatorcontrib>Zaba, Lisa C.</creatorcontrib><creatorcontrib>Moussai, Dariush</creatorcontrib><creatorcontrib>Suárez-Fariñas, Mayte</creatorcontrib><creatorcontrib>Kaporis, Helen</creatorcontrib><creatorcontrib>Fan, Linda</creatorcontrib><creatorcontrib>Pierson, Katherine C.</creatorcontrib><creatorcontrib>White, Traci R.</creatorcontrib><creatorcontrib>Pitts-Kiefer, Alexander</creatorcontrib><creatorcontrib>Fuentes-Duculan, Judilyn</creatorcontrib><creatorcontrib>Guttman-Yassky, Emma</creatorcontrib><creatorcontrib>Krueger, James G.</creatorcontrib><creatorcontrib>Lowes, Michelle A.</creatorcontrib><creatorcontrib>Carucci, John A.</creatorcontrib><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bluth, Mark J.</au><au>Zaba, Lisa C.</au><au>Moussai, Dariush</au><au>Suárez-Fariñas, Mayte</au><au>Kaporis, Helen</au><au>Fan, Linda</au><au>Pierson, Katherine C.</au><au>White, Traci R.</au><au>Pitts-Kiefer, Alexander</au><au>Fuentes-Duculan, Judilyn</au><au>Guttman-Yassky, Emma</au><au>Krueger, James G.</au><au>Lowes, Michelle A.</au><au>Carucci, John A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Myeloid dendritic cells from human squamous cell carcinoma are poor stimulators of T cell proliferation</atitle><jtitle>Journal of investigative dermatology</jtitle><date>2009-04-23</date><risdate>2009</risdate><volume>129</volume><issue>10</issue><spage>2451</spage><epage>2462</epage><pages>2451-2462</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><abstract>In order to determine the phenotype and function of myeloid DCs from human cutaneous squamous cell carcinoma (SCC), we studied their surface marker expression and allo-stimulatory potential ex vivo . There were abundant CD11c + myeloid DCs, as well as TNF and inducible nitric oxide synthase (iNOS)-producing DCs (TIP-DCs), in and around SCC tumor nests. Although myeloid DCs from SCC, adjacent non-tumor bearing skin, and normal skin, were phenotypically similar by flow cytometry, and there was a pronounced genomic signature of mature DCs in SCC, they showed different T cell stimulatory potential in an allo-MLR. Myeloid DCs from SCC were less potent stimulators of allogeneic T cell proliferation than DCs from non-tumor bearing skin. Culture with a DC-maturing cytokine cocktail (IL-1β, IL-6, TNF, and PGE 2 ) enhanced stimulatory potential in DCs from non-tumor-bearing skin, while SCC associated DCs remained poor stimulators of T cell proliferation. The microenvironment associated with SCC showed expression of TGFβ, IL-10 and VEGF-A, factors capable of suppressing DC function. These findings indicate that CD11c + /HLA-DR hi DCs from SCC are mature, but are not potent stimulators of T cell proliferation compared with phenotypically similar DCs isolated from non-tumor-bearing skin. Identification of mechanisms responsible for suppression of tumor-associated DCs may provide insight into evasion of immunosurveillance by SCC.</abstract><pmid>19387481</pmid><doi>10.1038/jid.2009.96</doi></addata></record>
fulltext fulltext
identifier ISSN: 0022-202X
ispartof Journal of investigative dermatology, 2009-04, Vol.129 (10), p.2451-2462
issn 0022-202X
1523-1747
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2846605
source EZB-FREE-00999 freely available EZB journals; ProQuest Central UK/Ireland; Alma/SFX Local Collection
title Myeloid dendritic cells from human squamous cell carcinoma are poor stimulators of T cell proliferation
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T17%3A54%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmedcentral&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Myeloid%20dendritic%20cells%20from%20human%20squamous%20cell%20carcinoma%20are%20poor%20stimulators%20of%20T%20cell%20proliferation&rft.jtitle=Journal%20of%20investigative%20dermatology&rft.au=Bluth,%20Mark%20J.&rft.date=2009-04-23&rft.volume=129&rft.issue=10&rft.spage=2451&rft.epage=2462&rft.pages=2451-2462&rft.issn=0022-202X&rft.eissn=1523-1747&rft_id=info:doi/10.1038/jid.2009.96&rft_dat=%3Cpubmedcentral%3Epubmedcentral_primary_oai_pubmedcentral_nih_gov_2846605%3C/pubmedcentral%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/19387481&rfr_iscdi=true