Cross-Talk-Free Dual-Color Fluorescence Cross-Correlation Spectroscopy for the Study of Enzyme Activity

We have developed an instrument for spectral cross-talk-free dual-color fluorescence cross-correlation spectroscopy (FCCS), which provides a readout modality for the study of enzyme activity in application areas such as high-throughput screening. Two spectrally distinct (∼250 nm) fluorophores, Cy3 a...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-02, Vol.82 (4), p.1401-1410
Hauptverfasser:	Lee, Wonbae, Lee, Yong-Ill, Lee, Jeonghoon, Davis, Lloyd M, Deininger, Prescott, Soper, Steven A
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Base Sequence Carbocyanines - metabolism Chemistry Color Deoxyribonucleic acid DNA DNA - genetics DNA - metabolism DNA-(Apurinic or Apyrimidinic Site) Lyase - metabolism Enzyme Assays - instrumentation Enzyme Assays - methods Enzymes Exact sciences and technology Fluorescence Fluorescence Resonance Energy Transfer Fluorescent Dyes - metabolism General, instrumentation Indoles - metabolism Reproducibility of Results Spectrometric and optical methods Spectrometry, Fluorescence - instrumentation Spectrometry, Fluorescence - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrum analysis Time Factors
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