Intrinsic Raman spectroscopy for quantitative biological spectroscopy part II: experimental applications

We demonstrate the effectiveness of intrinsic Raman spectroscopy (IRS) at reducing errors caused by absorption and scattering. Physical tissue models, solutions of varying absorption and scattering coefficients with known concentrations of Raman scatterers, are studied. We show significant improveme...

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Veröffentlicht in:	Optics express 2008-08, Vol.16 (17), p.12737-12745
Hauptverfasser:	Bechtel, Kate L, Shih, Wei-Chuan, Feld, Michael S
Format:	Artikel
Sprache:	eng
Schlagworte:	Biopolymers - chemistry Computer Simulation Light Models, Biological Models, Chemical Nephelometry and Turbidimetry - methods Scattering, Radiation Spectrum Analysis, Raman - methods
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creator	Bechtel, Kate L Shih, Wei-Chuan Feld, Michael S
description	We demonstrate the effectiveness of intrinsic Raman spectroscopy (IRS) at reducing errors caused by absorption and scattering. Physical tissue models, solutions of varying absorption and scattering coefficients with known concentrations of Raman scatterers, are studied. We show significant improvement in prediction error by implementing IRS to predict concentrations of Raman scatterers using both ordinary least squares regression (OLS) and partial least squares regression (PLS). In particular, we show that IRS provides a robust calibration model that does not increase in error when applied to samples with optical properties outside the range of calibration.
doi_str_mv	10.1364/OE.16.012737
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subjects	Biopolymers - chemistry Computer Simulation Light Models, Biological Models, Chemical Nephelometry and Turbidimetry - methods Scattering, Radiation Spectrum Analysis, Raman - methods
title	Intrinsic Raman spectroscopy for quantitative biological spectroscopy part II: experimental applications
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