A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation
We describe a new method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans w...
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| Veröffentlicht in: | Nature chemical biology 2010-04, Vol.6 (4), p.264-266 |
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| creator | Aebi, Markus Wang, Lai-Xi Schwarz, Flavio Huang, Wei Li, Cishan Schulz, Benjamin L Lizak, Christian Palumbo, Alessandro Numao, Shin Neri, Dario |
| description | We describe a new method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins. |
| doi_str_mv | 10.1038/nchembio.314 |
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The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. 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The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>20190762</pmid><doi>10.1038/nchembio.314</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/1647/334/2241/1474 631/61/338/318 631/92/458/1524 Biochemical Engineering Biochemistry Bioorganic Chemistry brief-communication Campylobacter jejuni Campylobacter jejuni - genetics Campylobacter jejuni - metabolism Cell Biology Chemical engineering Chemistry Chemistry and Materials Science Chemistry/Food Science E coli Enzymes Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Eukaryota - chemistry Eukaryota - metabolism Genetic Engineering Glycoproteins Glycoproteins - biosynthesis Glycoproteins - chemistry Glycosylation Research methodology |
| title | A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation |
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