Single-Molecule Spectroscopy and Imaging of Biomolecules in Living Cells
The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a single molecule were completed over two decades ago. Single-molecule spectroscopy has developed into a field that includes a wealth of experi...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2010-03, Vol.82 (6), p.2192-2203 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Lord, Samuel J Lee, Hsiao-lu D Moerner, W. E |
| description | The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a single molecule were completed over two decades ago. Single-molecule spectroscopy has developed into a field that includes a wealth of experiments at room temperature and inside living cells. The fast growth of single-molecule biophysics has resulted from its benefits in probing heterogeneous populations, one molecule at a time, as well as from advances in microscopes and detectors. This Perspective summarizes the field of live-cell imaging of single biomolecules. |
| doi_str_mv | 10.1021/ac9024889 |
| format | Article |
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| subjects | Analytical chemistry Animals Biological Transport Biophysics Biophysics - methods Cell Membrane - ultrastructure Cell Nucleolus - ultrastructure Cells Cellular Structures - metabolism Cellular Structures - ultrastructure Chemistry Cytoskeleton - ultrastructure Equipment Design Exact sciences and technology Fluorescence Resonance Energy Transfer Gene Expression Humans Microscopy - instrumentation Microscopy - methods Microscopy - trends Molecules Spectrum analysis Spectrum Analysis - methods Spectrum Analysis - trends |
| title | Single-Molecule Spectroscopy and Imaging of Biomolecules in Living Cells |
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