Identification and Functional Characterization of Monofunctional ent -Copalyl Diphosphate and ent -Kaurene Synthases in White Spruce Reveal Different Patterns for Diterpene Synthase Evolution for Primary and Secondary Metabolism in Gymnosperms

The biosynthesis of the tetracyclic diterpene ent-kaurene is a critical step in the general (primary) metabolism of gibberellin hormones. ent-Kaurene is formed by a two-step cyclization of geranylgeranyl diphosphate via the intermediate ent-copalyl diphosphate. In a lower land plant, the moss Physco...

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Veröffentlicht in:	Plant physiology (Bethesda) 2010-03, Vol.152 (3), p.1197-1208
Hauptverfasser:	Keeling, Christopher I., Dullat, Harpreet K., Yuen, Mack, Ralph, Steven G., Jancsik, Sharon, Bohlmann, Jörg
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkyl and Aryl Transferases - genetics Alkyl and Aryl Transferases - metabolism Amino Acid Sequence Angiosperms BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES Biological and medical sciences Biosynthesis Chromosomes, Artificial, Bacterial Cloning, Molecular Complementary DNA Conifers Diterpenes Diterpenes, Kaurane - biosynthesis DNA, Complementary - genetics DNA, Plant - genetics Enzymes Evolution, Molecular Expressed Sequence Tags Forestry Fundamental and applied biological sciences. Psychology Gibberellins Gymnosperms Introns Metabolism Molecular Sequence Data Mutagenesis, Site-Directed Phylogeny Picea - enzymology Picea - genetics Plant physiology and development Plant Proteins - genetics Plant Proteins - metabolism Sequence Alignment Sequence Analysis, DNA
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