Tubular Expression of KIM-1 Does not Predict Delayed Function After Transplantation
Injured epithelial cells of the proximal tubule upregulate the glycoprotein kidney injury molecule 1 (KIM-1), suggesting its potential as a biomarker of incipient kidney allograft injury. It is unknown whether KIM-1 expression changes in kidney allografts with delayed graft function (DGF), which oft...
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Veröffentlicht in: | Journal of the American Society of Nephrology 2010-03, Vol.21 (3), p.536-542 |
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creator | SCHRÖPPEL, Bernd KRÜGER, Bernd BONVENTRE, Joseph V ZHU WANG FARRIS, Alton B COLVIN, Robert B MURPHY, Barbara T VELLA, John P WALSH, Liron YEUNG, Melissa HARRIS, Shay GARRISON, Krista HIMMELFARB, Jonathan LERNER, Susan M BROMBERG, Jonathan S ZHANG, Ping L |
description | Injured epithelial cells of the proximal tubule upregulate the glycoprotein kidney injury molecule 1 (KIM-1), suggesting its potential as a biomarker of incipient kidney allograft injury. It is unknown whether KIM-1 expression changes in kidney allografts with delayed graft function (DGF), which often follows ischemia-reperfusion injury. Here, we prospectively measured KIM-1 RNA and protein expression in preperfusion biopsies of 30 living- and 85 deceased-donor kidneys and correlated the results with histologic and clinical outcomes after transplantation. We detected KIM-1 expression in 62% of deceased-donor kidneys and only 13% of living-donor kidneys (P < 0.0001). The level of KIM-1 expression before reperfusion correlated inversely with renal function at the time of procurement and correlated directly with the degree of interstitial fibrosis. Surprising, however, we did not detect a significant correlation between KIM-1 staining intensity and the occurrence of DGF. Our findings are consistent with a role for KIM-1 as an early indicator of tubular injury but do not support tissue KIM-1 measurement before transplantation to identify kidneys at risk for DGF. |
doi_str_mv | 10.1681/ASN.2009040390 |
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It is unknown whether KIM-1 expression changes in kidney allografts with delayed graft function (DGF), which often follows ischemia-reperfusion injury. Here, we prospectively measured KIM-1 RNA and protein expression in preperfusion biopsies of 30 living- and 85 deceased-donor kidneys and correlated the results with histologic and clinical outcomes after transplantation. We detected KIM-1 expression in 62% of deceased-donor kidneys and only 13% of living-donor kidneys (P < 0.0001). The level of KIM-1 expression before reperfusion correlated inversely with renal function at the time of procurement and correlated directly with the degree of interstitial fibrosis. Surprising, however, we did not detect a significant correlation between KIM-1 staining intensity and the occurrence of DGF. Our findings are consistent with a role for KIM-1 as an early indicator of tubular injury but do not support tissue KIM-1 measurement before transplantation to identify kidneys at risk for DGF.</description><identifier>ISSN: 1046-6673</identifier><identifier>EISSN: 1533-3450</identifier><identifier>DOI: 10.1681/ASN.2009040390</identifier><identifier>PMID: 20019169</identifier><identifier>CODEN: JASNEU</identifier><language>eng</language><publisher>Washington, DC: American Society of Nephrology</publisher><subject>Adolescent ; Adult ; Biological and medical sciences ; Biomarkers - metabolism ; Biopsy ; Cadaver ; Clinical Research ; Delayed Graft Function - diagnosis ; Delayed Graft Function - metabolism ; Delayed Graft Function - pathology ; Female ; Fibrosis ; Hepatitis A Virus Cellular Receptor 1 ; Humans ; Kidney - pathology ; Kidney Transplantation ; Living Donors ; Male ; Medical sciences ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - metabolism ; Middle Aged ; Nephrology. Urinary tract diseases ; Predictive Value of Tests ; Preoperative Care ; Receptors, Virus - genetics ; Receptors, Virus - metabolism ; Reperfusion Injury - diagnosis ; Reperfusion Injury - metabolism ; Reperfusion Injury - pathology ; Transplantation, Homologous ; Young Adult</subject><ispartof>Journal of the American Society of Nephrology, 2010-03, Vol.21 (3), p.536-542</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 by the American Society of Nephrology 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-cf917464513e0bc27a0abecefa95c6240cafc31e6fe813d6fbe42ea61d4184ab3</citedby><cites>FETCH-LOGICAL-c419t-cf917464513e0bc27a0abecefa95c6240cafc31e6fe813d6fbe42ea61d4184ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831861/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831861/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22483654$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20019169$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SCHRÖPPEL, Bernd</creatorcontrib><creatorcontrib>KRÜGER, Bernd</creatorcontrib><creatorcontrib>BONVENTRE, Joseph V</creatorcontrib><creatorcontrib>ZHU WANG</creatorcontrib><creatorcontrib>FARRIS, Alton B</creatorcontrib><creatorcontrib>COLVIN, Robert B</creatorcontrib><creatorcontrib>MURPHY, Barbara T</creatorcontrib><creatorcontrib>VELLA, John P</creatorcontrib><creatorcontrib>WALSH, Liron</creatorcontrib><creatorcontrib>YEUNG, Melissa</creatorcontrib><creatorcontrib>HARRIS, Shay</creatorcontrib><creatorcontrib>GARRISON, Krista</creatorcontrib><creatorcontrib>HIMMELFARB, Jonathan</creatorcontrib><creatorcontrib>LERNER, Susan M</creatorcontrib><creatorcontrib>BROMBERG, Jonathan S</creatorcontrib><creatorcontrib>ZHANG, Ping L</creatorcontrib><title>Tubular Expression of KIM-1 Does not Predict Delayed Function After Transplantation</title><title>Journal of the American Society of Nephrology</title><addtitle>J Am Soc Nephrol</addtitle><description>Injured epithelial cells of the proximal tubule upregulate the glycoprotein kidney injury molecule 1 (KIM-1), suggesting its potential as a biomarker of incipient kidney allograft injury. It is unknown whether KIM-1 expression changes in kidney allografts with delayed graft function (DGF), which often follows ischemia-reperfusion injury. Here, we prospectively measured KIM-1 RNA and protein expression in preperfusion biopsies of 30 living- and 85 deceased-donor kidneys and correlated the results with histologic and clinical outcomes after transplantation. We detected KIM-1 expression in 62% of deceased-donor kidneys and only 13% of living-donor kidneys (P < 0.0001). The level of KIM-1 expression before reperfusion correlated inversely with renal function at the time of procurement and correlated directly with the degree of interstitial fibrosis. Surprising, however, we did not detect a significant correlation between KIM-1 staining intensity and the occurrence of DGF. Our findings are consistent with a role for KIM-1 as an early indicator of tubular injury but do not support tissue KIM-1 measurement before transplantation to identify kidneys at risk for DGF.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - metabolism</subject><subject>Biopsy</subject><subject>Cadaver</subject><subject>Clinical Research</subject><subject>Delayed Graft Function - diagnosis</subject><subject>Delayed Graft Function - metabolism</subject><subject>Delayed Graft Function - pathology</subject><subject>Female</subject><subject>Fibrosis</subject><subject>Hepatitis A Virus Cellular Receptor 1</subject><subject>Humans</subject><subject>Kidney - pathology</subject><subject>Kidney Transplantation</subject><subject>Living Donors</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Middle Aged</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Predictive Value of Tests</subject><subject>Preoperative Care</subject><subject>Receptors, Virus - genetics</subject><subject>Receptors, Virus - metabolism</subject><subject>Reperfusion Injury - diagnosis</subject><subject>Reperfusion Injury - metabolism</subject><subject>Reperfusion Injury - pathology</subject><subject>Transplantation, Homologous</subject><subject>Young Adult</subject><issn>1046-6673</issn><issn>1533-3450</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkctP3DAQxq0KVB7ttcfKF9RTFk_sOMml0mp5ikcrsT1bE2dcgrLxYicV_Pf1igXak0f2b77xfB9jX0DMQFdwPL-7neVC1EIJWYsPbB8KKTOpCrGTaqF0pnUp99hBjA9CQJGX5Ue2lzqgBl3vs7vl1Ew9Bn76tA4UY-cH7h2_urzJgJ94inzwI_8ZqO3syE-ox2dq-dk02HGDzt1IgS8DDnHd4zDi5vYT23XYR_q8PQ_Zr7PT5eIiu_5xfrmYX2dWQT1m1tVQKq0KkCQam5cosCFLDuvC6lwJi85KIO2oAtlq15DKCTW0CiqFjTxk319011OzotbSMAbszTp0KwzPxmNn_n8Zunvz2_8xeSWh0pAEvm0Fgn-cKI5m1UVLfdqE_BRNKaWuAVSRyNkLaYOPMZB7mwLCbIIwKQjzHkRq-Prv397wV-cTcLQFMFrsXbLQdvGdy1UldaHkXzWckdQ</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>SCHRÖPPEL, Bernd</creator><creator>KRÜGER, Bernd</creator><creator>BONVENTRE, Joseph V</creator><creator>ZHU WANG</creator><creator>FARRIS, Alton B</creator><creator>COLVIN, Robert B</creator><creator>MURPHY, Barbara T</creator><creator>VELLA, John P</creator><creator>WALSH, Liron</creator><creator>YEUNG, Melissa</creator><creator>HARRIS, Shay</creator><creator>GARRISON, Krista</creator><creator>HIMMELFARB, Jonathan</creator><creator>LERNER, Susan M</creator><creator>BROMBERG, Jonathan S</creator><creator>ZHANG, Ping L</creator><general>American Society of Nephrology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20100301</creationdate><title>Tubular Expression of KIM-1 Does not Predict Delayed Function After Transplantation</title><author>SCHRÖPPEL, Bernd ; KRÜGER, Bernd ; BONVENTRE, Joseph V ; ZHU WANG ; FARRIS, Alton B ; COLVIN, Robert B ; MURPHY, Barbara T ; VELLA, John P ; WALSH, Liron ; YEUNG, Melissa ; HARRIS, Shay ; GARRISON, Krista ; HIMMELFARB, Jonathan ; LERNER, Susan M ; BROMBERG, Jonathan S ; ZHANG, Ping L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-cf917464513e0bc27a0abecefa95c6240cafc31e6fe813d6fbe42ea61d4184ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - metabolism</topic><topic>Biopsy</topic><topic>Cadaver</topic><topic>Clinical Research</topic><topic>Delayed Graft Function - diagnosis</topic><topic>Delayed Graft Function - metabolism</topic><topic>Delayed Graft Function - pathology</topic><topic>Female</topic><topic>Fibrosis</topic><topic>Hepatitis A Virus Cellular Receptor 1</topic><topic>Humans</topic><topic>Kidney - pathology</topic><topic>Kidney Transplantation</topic><topic>Living Donors</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Middle Aged</topic><topic>Nephrology. 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It is unknown whether KIM-1 expression changes in kidney allografts with delayed graft function (DGF), which often follows ischemia-reperfusion injury. Here, we prospectively measured KIM-1 RNA and protein expression in preperfusion biopsies of 30 living- and 85 deceased-donor kidneys and correlated the results with histologic and clinical outcomes after transplantation. We detected KIM-1 expression in 62% of deceased-donor kidneys and only 13% of living-donor kidneys (P < 0.0001). The level of KIM-1 expression before reperfusion correlated inversely with renal function at the time of procurement and correlated directly with the degree of interstitial fibrosis. Surprising, however, we did not detect a significant correlation between KIM-1 staining intensity and the occurrence of DGF. Our findings are consistent with a role for KIM-1 as an early indicator of tubular injury but do not support tissue KIM-1 measurement before transplantation to identify kidneys at risk for DGF.</abstract><cop>Washington, DC</cop><pub>American Society of Nephrology</pub><pmid>20019169</pmid><doi>10.1681/ASN.2009040390</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adolescent Adult Biological and medical sciences Biomarkers - metabolism Biopsy Cadaver Clinical Research Delayed Graft Function - diagnosis Delayed Graft Function - metabolism Delayed Graft Function - pathology Female Fibrosis Hepatitis A Virus Cellular Receptor 1 Humans Kidney - pathology Kidney Transplantation Living Donors Male Medical sciences Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Middle Aged Nephrology. Urinary tract diseases Predictive Value of Tests Preoperative Care Receptors, Virus - genetics Receptors, Virus - metabolism Reperfusion Injury - diagnosis Reperfusion Injury - metabolism Reperfusion Injury - pathology Transplantation, Homologous Young Adult |
title | Tubular Expression of KIM-1 Does not Predict Delayed Function After Transplantation |
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