Tubular Expression of KIM-1 Does not Predict Delayed Function After Transplantation

Injured epithelial cells of the proximal tubule upregulate the glycoprotein kidney injury molecule 1 (KIM-1), suggesting its potential as a biomarker of incipient kidney allograft injury. It is unknown whether KIM-1 expression changes in kidney allografts with delayed graft function (DGF), which oft...

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Veröffentlicht in:Journal of the American Society of Nephrology 2010-03, Vol.21 (3), p.536-542
Hauptverfasser: SCHRÖPPEL, Bernd, KRÜGER, Bernd, BONVENTRE, Joseph V, ZHU WANG, FARRIS, Alton B, COLVIN, Robert B, MURPHY, Barbara T, VELLA, John P, WALSH, Liron, YEUNG, Melissa, HARRIS, Shay, GARRISON, Krista, HIMMELFARB, Jonathan, LERNER, Susan M, BROMBERG, Jonathan S, ZHANG, Ping L
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container_end_page 542
container_issue 3
container_start_page 536
container_title Journal of the American Society of Nephrology
container_volume 21
creator SCHRÖPPEL, Bernd
KRÜGER, Bernd
BONVENTRE, Joseph V
ZHU WANG
FARRIS, Alton B
COLVIN, Robert B
MURPHY, Barbara T
VELLA, John P
WALSH, Liron
YEUNG, Melissa
HARRIS, Shay
GARRISON, Krista
HIMMELFARB, Jonathan
LERNER, Susan M
BROMBERG, Jonathan S
ZHANG, Ping L
description Injured epithelial cells of the proximal tubule upregulate the glycoprotein kidney injury molecule 1 (KIM-1), suggesting its potential as a biomarker of incipient kidney allograft injury. It is unknown whether KIM-1 expression changes in kidney allografts with delayed graft function (DGF), which often follows ischemia-reperfusion injury. Here, we prospectively measured KIM-1 RNA and protein expression in preperfusion biopsies of 30 living- and 85 deceased-donor kidneys and correlated the results with histologic and clinical outcomes after transplantation. We detected KIM-1 expression in 62% of deceased-donor kidneys and only 13% of living-donor kidneys (P < 0.0001). The level of KIM-1 expression before reperfusion correlated inversely with renal function at the time of procurement and correlated directly with the degree of interstitial fibrosis. Surprising, however, we did not detect a significant correlation between KIM-1 staining intensity and the occurrence of DGF. Our findings are consistent with a role for KIM-1 as an early indicator of tubular injury but do not support tissue KIM-1 measurement before transplantation to identify kidneys at risk for DGF.
doi_str_mv 10.1681/ASN.2009040390
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It is unknown whether KIM-1 expression changes in kidney allografts with delayed graft function (DGF), which often follows ischemia-reperfusion injury. Here, we prospectively measured KIM-1 RNA and protein expression in preperfusion biopsies of 30 living- and 85 deceased-donor kidneys and correlated the results with histologic and clinical outcomes after transplantation. We detected KIM-1 expression in 62% of deceased-donor kidneys and only 13% of living-donor kidneys (P &lt; 0.0001). The level of KIM-1 expression before reperfusion correlated inversely with renal function at the time of procurement and correlated directly with the degree of interstitial fibrosis. Surprising, however, we did not detect a significant correlation between KIM-1 staining intensity and the occurrence of DGF. 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Urinary tract diseases</subject><subject>Predictive Value of Tests</subject><subject>Preoperative Care</subject><subject>Receptors, Virus - genetics</subject><subject>Receptors, Virus - metabolism</subject><subject>Reperfusion Injury - diagnosis</subject><subject>Reperfusion Injury - metabolism</subject><subject>Reperfusion Injury - pathology</subject><subject>Transplantation, Homologous</subject><subject>Young Adult</subject><issn>1046-6673</issn><issn>1533-3450</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkctP3DAQxq0KVB7ttcfKF9RTFk_sOMml0mp5ikcrsT1bE2dcgrLxYicV_Pf1igXak0f2b77xfB9jX0DMQFdwPL-7neVC1EIJWYsPbB8KKTOpCrGTaqF0pnUp99hBjA9CQJGX5Ue2lzqgBl3vs7vl1Ew9Bn76tA4UY-cH7h2_urzJgJ94inzwI_8ZqO3syE-ox2dq-dk02HGDzt1IgS8DDnHd4zDi5vYT23XYR_q8PQ_Zr7PT5eIiu_5xfrmYX2dWQT1m1tVQKq0KkCQam5cosCFLDuvC6lwJi85KIO2oAtlq15DKCTW0CiqFjTxk319011OzotbSMAbszTp0KwzPxmNn_n8Zunvz2_8xeSWh0pAEvm0Fgn-cKI5m1UVLfdqE_BRNKaWuAVSRyNkLaYOPMZB7mwLCbIIwKQjzHkRq-Prv397wV-cTcLQFMFrsXbLQdvGdy1UldaHkXzWckdQ</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>SCHRÖPPEL, Bernd</creator><creator>KRÜGER, Bernd</creator><creator>BONVENTRE, Joseph V</creator><creator>ZHU WANG</creator><creator>FARRIS, Alton B</creator><creator>COLVIN, Robert B</creator><creator>MURPHY, Barbara T</creator><creator>VELLA, John P</creator><creator>WALSH, Liron</creator><creator>YEUNG, Melissa</creator><creator>HARRIS, Shay</creator><creator>GARRISON, Krista</creator><creator>HIMMELFARB, Jonathan</creator><creator>LERNER, Susan M</creator><creator>BROMBERG, Jonathan S</creator><creator>ZHANG, Ping L</creator><general>American Society of Nephrology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20100301</creationdate><title>Tubular Expression of KIM-1 Does not Predict Delayed Function After Transplantation</title><author>SCHRÖPPEL, Bernd ; KRÜGER, Bernd ; BONVENTRE, Joseph V ; ZHU WANG ; FARRIS, Alton B ; COLVIN, Robert B ; MURPHY, Barbara T ; VELLA, John P ; WALSH, Liron ; YEUNG, Melissa ; HARRIS, Shay ; GARRISON, Krista ; HIMMELFARB, Jonathan ; LERNER, Susan M ; BROMBERG, Jonathan S ; ZHANG, Ping L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-cf917464513e0bc27a0abecefa95c6240cafc31e6fe813d6fbe42ea61d4184ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - metabolism</topic><topic>Biopsy</topic><topic>Cadaver</topic><topic>Clinical Research</topic><topic>Delayed Graft Function - diagnosis</topic><topic>Delayed Graft Function - metabolism</topic><topic>Delayed Graft Function - pathology</topic><topic>Female</topic><topic>Fibrosis</topic><topic>Hepatitis A Virus Cellular Receptor 1</topic><topic>Humans</topic><topic>Kidney - pathology</topic><topic>Kidney Transplantation</topic><topic>Living Donors</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Middle Aged</topic><topic>Nephrology. 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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects Adolescent
Adult
Biological and medical sciences
Biomarkers - metabolism
Biopsy
Cadaver
Clinical Research
Delayed Graft Function - diagnosis
Delayed Graft Function - metabolism
Delayed Graft Function - pathology
Female
Fibrosis
Hepatitis A Virus Cellular Receptor 1
Humans
Kidney - pathology
Kidney Transplantation
Living Donors
Male
Medical sciences
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Middle Aged
Nephrology. Urinary tract diseases
Predictive Value of Tests
Preoperative Care
Receptors, Virus - genetics
Receptors, Virus - metabolism
Reperfusion Injury - diagnosis
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
Transplantation, Homologous
Young Adult
title Tubular Expression of KIM-1 Does not Predict Delayed Function After Transplantation
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