Anti-Inflammatory Protein of Schistosoma japonicum Directs the Differentiation of the WEHI-3B JCS Cells and Mouse Bone Marrow Cells to Macrophages
Sj16 is an anti-inflammatory protein identified from Schistosoma japonicum. Our previous studies showed that recombinant Sj16 (rSj16) could suppress host's inflammatory responses and inhibit macrophage maturation. In the present study, the effects of rSj16 on the differentiation of the murine m...
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| creator | Yang, Linlin Hu, Shaomin Wu, Zhongdao Mak, Nai Ki Leung, Kwok Nam Fung, Ming Chui |
| description | Sj16 is an anti-inflammatory protein identified from Schistosoma japonicum. Our previous studies showed that recombinant Sj16 (rSj16) could suppress host's inflammatory responses and inhibit macrophage maturation. In the present study, the effects of rSj16 on the differentiation of the murine myeloid leukemia WEHI-3B JCS cell line and on mouse hematopoiesis were investigated. Our data demonstrated that rSj16 expressed and purified from Escherichia coli could suppress the proliferation of the WEHI-3B JCS cells in a time- and concentration-dependent manner, while not affect the viability of the cells. Further studies indicated that rSj16 induced macrophage differentiation of the WEHI-3B JCS cells, and arrested the cell cycle in the G1/G0 and G2/M phases. The macrophage differentiation of the rSj16-treated WEHI-3B JCS cells was confirmed by their expression of macrophage specific antigen F4/80 and phagocytic activity. Furthermore, our results revealed that rSj16 biased the colony formation of mouse bone marrow cells towards macrophage linage. |
| doi_str_mv | 10.1155/2010/867368 |
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Our previous studies showed that recombinant Sj16 (rSj16) could suppress host's inflammatory responses and inhibit macrophage maturation. In the present study, the effects of rSj16 on the differentiation of the murine myeloid leukemia WEHI-3B JCS cell line and on mouse hematopoiesis were investigated. Our data demonstrated that rSj16 expressed and purified from Escherichia coli could suppress the proliferation of the WEHI-3B JCS cells in a time- and concentration-dependent manner, while not affect the viability of the cells. Further studies indicated that rSj16 induced macrophage differentiation of the WEHI-3B JCS cells, and arrested the cell cycle in the G1/G0 and G2/M phases. The macrophage differentiation of the rSj16-treated WEHI-3B JCS cells was confirmed by their expression of macrophage specific antigen F4/80 and phagocytic activity. Furthermore, our results revealed that rSj16 biased the colony formation of mouse bone marrow cells towards macrophage linage.</description><identifier>ISSN: 1110-7243</identifier><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 1110-7251</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2010/867368</identifier><identifier>PMID: 20204135</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Animals ; Antigens, Differentiation - metabolism ; Antigens, Helminth - genetics ; Antigens, Helminth - immunology ; Antigens, Helminth - metabolism ; Apoptosis ; Biomedical research ; Biotechnology ; Bone marrow ; Bone Marrow Cells ; Cell adhesion & migration ; Cell Adhesion - immunology ; Cell cycle ; Cell Cycle - immunology ; Cell Differentiation - immunology ; Cell Line, Tumor ; Cell Proliferation ; Colonies & territories ; Cytokines - metabolism ; Differentiation ; E coli ; Escherichia coli ; Female ; Helminth Proteins - genetics ; Helminth Proteins - immunology ; Helminth Proteins - metabolism ; Histocytochemistry ; Immune system ; Laboratory animals ; Macrophages ; Macrophages - cytology ; Macrophages - immunology ; Macrophages - metabolism ; Mice ; Mice, Inbred BALB C ; Phagocytosis ; Phases ; Proteins ; Receptors, Chemokine - metabolism ; Recombinant ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Recombinant Proteins - metabolism ; Schistosoma japonicum ; Schistosoma japonicum - genetics ; Schistosoma japonicum - immunology ; Studies</subject><ispartof>BioMed research international, 2010-01, Vol.2010 (2010), p.1-13</ispartof><rights>Copyright © 2010</rights><rights>Copyright © 2010 Shaomin Hu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2010 Shaomin Hu et al. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c482t-88621001c243c2d368ee0b47941cb16bccd864371108241e18099baeda2eade93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831513/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831513/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20204135$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Morales-Montor, Jorge</contributor><creatorcontrib>Yang, Linlin</creatorcontrib><creatorcontrib>Hu, Shaomin</creatorcontrib><creatorcontrib>Wu, Zhongdao</creatorcontrib><creatorcontrib>Mak, Nai Ki</creatorcontrib><creatorcontrib>Leung, Kwok Nam</creatorcontrib><creatorcontrib>Fung, Ming Chui</creatorcontrib><title>Anti-Inflammatory Protein of Schistosoma japonicum Directs the Differentiation of the WEHI-3B JCS Cells and Mouse Bone Marrow Cells to Macrophages</title><title>BioMed research international</title><addtitle>J Biomed Biotechnol</addtitle><description>Sj16 is an anti-inflammatory protein identified from Schistosoma japonicum. Our previous studies showed that recombinant Sj16 (rSj16) could suppress host's inflammatory responses and inhibit macrophage maturation. In the present study, the effects of rSj16 on the differentiation of the murine myeloid leukemia WEHI-3B JCS cell line and on mouse hematopoiesis were investigated. Our data demonstrated that rSj16 expressed and purified from Escherichia coli could suppress the proliferation of the WEHI-3B JCS cells in a time- and concentration-dependent manner, while not affect the viability of the cells. Further studies indicated that rSj16 induced macrophage differentiation of the WEHI-3B JCS cells, and arrested the cell cycle in the G1/G0 and G2/M phases. The macrophage differentiation of the rSj16-treated WEHI-3B JCS cells was confirmed by their expression of macrophage specific antigen F4/80 and phagocytic activity. Furthermore, our results revealed that rSj16 biased the colony formation of mouse bone marrow cells towards macrophage linage.</description><subject>Animals</subject><subject>Antigens, Differentiation - metabolism</subject><subject>Antigens, Helminth - genetics</subject><subject>Antigens, Helminth - immunology</subject><subject>Antigens, Helminth - metabolism</subject><subject>Apoptosis</subject><subject>Biomedical research</subject><subject>Biotechnology</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells</subject><subject>Cell adhesion & migration</subject><subject>Cell Adhesion - immunology</subject><subject>Cell cycle</subject><subject>Cell Cycle - immunology</subject><subject>Cell Differentiation - immunology</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Colonies & territories</subject><subject>Cytokines - metabolism</subject><subject>Differentiation</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Female</subject><subject>Helminth Proteins - genetics</subject><subject>Helminth Proteins - immunology</subject><subject>Helminth Proteins - metabolism</subject><subject>Histocytochemistry</subject><subject>Immune system</subject><subject>Laboratory animals</subject><subject>Macrophages</subject><subject>Macrophages - cytology</subject><subject>Macrophages - immunology</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Phagocytosis</subject><subject>Phases</subject><subject>Proteins</subject><subject>Receptors, Chemokine - metabolism</subject><subject>Recombinant</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant Proteins - metabolism</subject><subject>Schistosoma japonicum</subject><subject>Schistosoma japonicum - genetics</subject><subject>Schistosoma japonicum - immunology</subject><subject>Studies</subject><issn>1110-7243</issn><issn>2314-6133</issn><issn>1110-7251</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkktvEzEUhUcIRB-wYg2y2IBAQ339Gs8GqQ2FBrUCqSCWluO50ziaGQd70qp_g1-MQ0IELOjKj_v5SOf4FMUToG8ApDxiFOiRVhVX-l6xDwC0rJiE-7u94HvFQUoLSqHSqn5Y7DHKqAAu94sfx8Poy-nQdrbv7RjiLfkcw4h-IKEll27u0xhS6C1Z2GUYvFv15J2P6MZExjnmfdtixCxiRx9-PVpffzs9m5b8hHycXJIJdl0idmjIRVglJCdhQHJhYww329kY8tnFsJzbK0yPiget7RI-3q6Hxdf3p18mZ-X5pw_TyfF56YRmY6m1YpAtuezPsSa7R6QzUdUC3AzUzLlGK8GrnIFmAhA0reuZxcYytA3W_LB4u9FdrmY9Ni6biLYzy-h7G29NsN78PRn83FyFa8M0Bwk8C7zYCsTwfYVpNL1PLjuyA2anphKiAi0U3E1yrirBQGTy5X9JUIIxlX9cZvT5P-girOKQIzNaKqgoEypDrzdQjjeliO3OH1Czro9Z18ds6pPpZ39GsmN_9yUDrzbA3A-NvfF3qD3dwJgRbO0OllQoKflPk6PVng</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Yang, Linlin</creator><creator>Hu, Shaomin</creator><creator>Wu, Zhongdao</creator><creator>Mak, Nai Ki</creator><creator>Leung, Kwok Nam</creator><creator>Fung, Ming Chui</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7U5</scope><scope>F28</scope><scope>L7M</scope><scope>7X8</scope><scope>7T5</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>5PM</scope></search><sort><creationdate>20100101</creationdate><title>Anti-Inflammatory Protein of Schistosoma japonicum Directs the Differentiation of the WEHI-3B JCS Cells and Mouse Bone Marrow Cells to Macrophages</title><author>Yang, Linlin ; Hu, Shaomin ; Wu, Zhongdao ; Mak, Nai Ki ; Leung, Kwok Nam ; Fung, Ming Chui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-88621001c243c2d368ee0b47941cb16bccd864371108241e18099baeda2eade93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Antigens, Differentiation - 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Our previous studies showed that recombinant Sj16 (rSj16) could suppress host's inflammatory responses and inhibit macrophage maturation. In the present study, the effects of rSj16 on the differentiation of the murine myeloid leukemia WEHI-3B JCS cell line and on mouse hematopoiesis were investigated. Our data demonstrated that rSj16 expressed and purified from Escherichia coli could suppress the proliferation of the WEHI-3B JCS cells in a time- and concentration-dependent manner, while not affect the viability of the cells. Further studies indicated that rSj16 induced macrophage differentiation of the WEHI-3B JCS cells, and arrested the cell cycle in the G1/G0 and G2/M phases. The macrophage differentiation of the rSj16-treated WEHI-3B JCS cells was confirmed by their expression of macrophage specific antigen F4/80 and phagocytic activity. Furthermore, our results revealed that rSj16 biased the colony formation of mouse bone marrow cells towards macrophage linage.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>20204135</pmid><doi>10.1155/2010/867368</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antigens, Differentiation - metabolism Antigens, Helminth - genetics Antigens, Helminth - immunology Antigens, Helminth - metabolism Apoptosis Biomedical research Biotechnology Bone marrow Bone Marrow Cells Cell adhesion & migration Cell Adhesion - immunology Cell cycle Cell Cycle - immunology Cell Differentiation - immunology Cell Line, Tumor Cell Proliferation Colonies & territories Cytokines - metabolism Differentiation E coli Escherichia coli Female Helminth Proteins - genetics Helminth Proteins - immunology Helminth Proteins - metabolism Histocytochemistry Immune system Laboratory animals Macrophages Macrophages - cytology Macrophages - immunology Macrophages - metabolism Mice Mice, Inbred BALB C Phagocytosis Phases Proteins Receptors, Chemokine - metabolism Recombinant Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - metabolism Schistosoma japonicum Schistosoma japonicum - genetics Schistosoma japonicum - immunology Studies |
| title | Anti-Inflammatory Protein of Schistosoma japonicum Directs the Differentiation of the WEHI-3B JCS Cells and Mouse Bone Marrow Cells to Macrophages |
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