Synchronous Reinitiation of Chromosome Replication in E. coli B/r after Nalidixic Acid Treatment
Nalidixic acid has been used to interrupt DNA synthesis in exponentially growing cultures of E. coli B/r. Within three minutes after removal of the nalidixic acid block, premature rounds of chromosome replication are initiated at the normal origin of vegetative DNA synthesis. The new growing forks t...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1970-06, Vol.66 (2), p.365-369 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 369 |
|---|---|
| container_issue | 2 |
| container_start_page | 365 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 66 |
| creator | Ward, C. B. Hane, M. W. Glaser, D. A. |
| description | Nalidixic acid has been used to interrupt DNA synthesis in exponentially growing cultures of E. coli B/r. Within three minutes after removal of the nalidixic acid block, premature rounds of chromosome replication are initiated at the normal origin of vegetative DNA synthesis. The new growing forks traverse the chromosome in about 40 minutes, the normal replication time. These rounds continue synchronously into the second generation after removal of the nalidixic acid. These phenomena are similar in some respect to the premature initiation of chromosomal replication observed after thymine starvation or after release of thermo-sensitive DNA synthesis mutants from the restrictive temperature that prevents DNA synthesis. |
| doi_str_mv | 10.1073/pnas.66.2.365 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_283053</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>60063</jstor_id><sourcerecordid>60063</sourcerecordid><originalsourceid>FETCH-LOGICAL-c451t-452b027dba01efa7a68fbf76f030c4effee56206f98e9d679c41392bfcb5407d3</originalsourceid><addsrcrecordid>eNp9kbFv1DAUhy1EVY7CyIJA8lK2pM-x4yQDQzkVilSBBGU2jmNTV459tX1V-9_jU45SFiYP3_fze3o_hF4RqAl09GTjZao5r5ua8vYJWhEYSMXZAE_RCqDpqp417Bl6ntI1AAxtD4fokA2k7Xu-Qj-_33t1FYMP24S_aetttjLb4HEweF3AHFKYdUEbZ9VCrMdnNVbBWfzhJGJpso74i3R2sndW4VNlJ3wZtcyz9vkFOjDSJf1y_x6hHx_PLtfn1cXXT5_XpxeVYi3JFWubsSw7jRKINrKTvDej6bgBCoppY7RueQPcDL0eJt4NihE6NKNRY8ugm-gRer_8u9mOs55UGR2lE5toZxnvRZBW_Eu8vRK_wq1oegotLfl3-3wMN1udsphtUto56XW5jegZZ1AuXMRqEVUMKUVtHmYQELtGxK4RwbloxOK_fbzYg72voPA3e76L_aGP4sf_wcJsncv6Lhfv9eJdpxziXxGAU_ob5oKpmg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>84640365</pqid></control><display><type>article</type><title>Synchronous Reinitiation of Chromosome Replication in E. coli B/r after Nalidixic Acid Treatment</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Ward, C. B. ; Hane, M. W. ; Glaser, D. A.</creator><creatorcontrib>Ward, C. B. ; Hane, M. W. ; Glaser, D. A.</creatorcontrib><description>Nalidixic acid has been used to interrupt DNA synthesis in exponentially growing cultures of E. coli B/r. Within three minutes after removal of the nalidixic acid block, premature rounds of chromosome replication are initiated at the normal origin of vegetative DNA synthesis. The new growing forks traverse the chromosome in about 40 minutes, the normal replication time. These rounds continue synchronously into the second generation after removal of the nalidixic acid. These phenomena are similar in some respect to the premature initiation of chromosomal replication observed after thymine starvation or after release of thermo-sensitive DNA synthesis mutants from the restrictive temperature that prevents DNA synthesis.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.66.2.365</identifier><identifier>PMID: 4915886</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Acid treatment ; Amino acids ; Biological Sciences: Genetics ; Cell growth ; Chromosome Mapping ; Chromosomes ; Chromosomes, Bacterial - drug effects ; Culture Techniques ; DNA ; DNA Replication ; DNA, Bacterial - biosynthesis ; Escherichia coli - drug effects ; Escherichia coli - metabolism ; Genetic mutation ; Guanidines - pharmacology ; Mutation ; Nalidixic Acid - pharmacology ; Nitroso Compounds - pharmacology ; Salts ; Starvation ; Time Factors ; Washing</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1970-06, Vol.66 (2), p.365-369</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-452b027dba01efa7a68fbf76f030c4effee56206f98e9d679c41392bfcb5407d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/66/2.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/60063$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/60063$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,729,782,786,805,887,27931,27932,53798,53800,58024,58257</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4915886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ward, C. B.</creatorcontrib><creatorcontrib>Hane, M. W.</creatorcontrib><creatorcontrib>Glaser, D. A.</creatorcontrib><title>Synchronous Reinitiation of Chromosome Replication in E. coli B/r after Nalidixic Acid Treatment</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Nalidixic acid has been used to interrupt DNA synthesis in exponentially growing cultures of E. coli B/r. Within three minutes after removal of the nalidixic acid block, premature rounds of chromosome replication are initiated at the normal origin of vegetative DNA synthesis. The new growing forks traverse the chromosome in about 40 minutes, the normal replication time. These rounds continue synchronously into the second generation after removal of the nalidixic acid. These phenomena are similar in some respect to the premature initiation of chromosomal replication observed after thymine starvation or after release of thermo-sensitive DNA synthesis mutants from the restrictive temperature that prevents DNA synthesis.</description><subject>Acid treatment</subject><subject>Amino acids</subject><subject>Biological Sciences: Genetics</subject><subject>Cell growth</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Chromosomes, Bacterial - drug effects</subject><subject>Culture Techniques</subject><subject>DNA</subject><subject>DNA Replication</subject><subject>DNA, Bacterial - biosynthesis</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - metabolism</subject><subject>Genetic mutation</subject><subject>Guanidines - pharmacology</subject><subject>Mutation</subject><subject>Nalidixic Acid - pharmacology</subject><subject>Nitroso Compounds - pharmacology</subject><subject>Salts</subject><subject>Starvation</subject><subject>Time Factors</subject><subject>Washing</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kbFv1DAUhy1EVY7CyIJA8lK2pM-x4yQDQzkVilSBBGU2jmNTV459tX1V-9_jU45SFiYP3_fze3o_hF4RqAl09GTjZao5r5ua8vYJWhEYSMXZAE_RCqDpqp417Bl6ntI1AAxtD4fokA2k7Xu-Qj-_33t1FYMP24S_aetttjLb4HEweF3AHFKYdUEbZ9VCrMdnNVbBWfzhJGJpso74i3R2sndW4VNlJ3wZtcyz9vkFOjDSJf1y_x6hHx_PLtfn1cXXT5_XpxeVYi3JFWubsSw7jRKINrKTvDej6bgBCoppY7RueQPcDL0eJt4NihE6NKNRY8ugm-gRer_8u9mOs55UGR2lE5toZxnvRZBW_Eu8vRK_wq1oegotLfl3-3wMN1udsphtUto56XW5jegZZ1AuXMRqEVUMKUVtHmYQELtGxK4RwbloxOK_fbzYg72voPA3e76L_aGP4sf_wcJsncv6Lhfv9eJdpxziXxGAU_ob5oKpmg</recordid><startdate>19700601</startdate><enddate>19700601</enddate><creator>Ward, C. B.</creator><creator>Hane, M. W.</creator><creator>Glaser, D. A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19700601</creationdate><title>Synchronous Reinitiation of Chromosome Replication in E. coli B/r after Nalidixic Acid Treatment</title><author>Ward, C. B. ; Hane, M. W. ; Glaser, D. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-452b027dba01efa7a68fbf76f030c4effee56206f98e9d679c41392bfcb5407d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Acid treatment</topic><topic>Amino acids</topic><topic>Biological Sciences: Genetics</topic><topic>Cell growth</topic><topic>Chromosome Mapping</topic><topic>Chromosomes</topic><topic>Chromosomes, Bacterial - drug effects</topic><topic>Culture Techniques</topic><topic>DNA</topic><topic>DNA Replication</topic><topic>DNA, Bacterial - biosynthesis</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - metabolism</topic><topic>Genetic mutation</topic><topic>Guanidines - pharmacology</topic><topic>Mutation</topic><topic>Nalidixic Acid - pharmacology</topic><topic>Nitroso Compounds - pharmacology</topic><topic>Salts</topic><topic>Starvation</topic><topic>Time Factors</topic><topic>Washing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ward, C. B.</creatorcontrib><creatorcontrib>Hane, M. W.</creatorcontrib><creatorcontrib>Glaser, D. A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ward, C. B.</au><au>Hane, M. W.</au><au>Glaser, D. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synchronous Reinitiation of Chromosome Replication in E. coli B/r after Nalidixic Acid Treatment</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1970-06-01</date><risdate>1970</risdate><volume>66</volume><issue>2</issue><spage>365</spage><epage>369</epage><pages>365-369</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Nalidixic acid has been used to interrupt DNA synthesis in exponentially growing cultures of E. coli B/r. Within three minutes after removal of the nalidixic acid block, premature rounds of chromosome replication are initiated at the normal origin of vegetative DNA synthesis. The new growing forks traverse the chromosome in about 40 minutes, the normal replication time. These rounds continue synchronously into the second generation after removal of the nalidixic acid. These phenomena are similar in some respect to the premature initiation of chromosomal replication observed after thymine starvation or after release of thermo-sensitive DNA synthesis mutants from the restrictive temperature that prevents DNA synthesis.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>4915886</pmid><doi>10.1073/pnas.66.2.365</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1970-06, Vol.66 (2), p.365-369 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_283053 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Acid treatment Amino acids Biological Sciences: Genetics Cell growth Chromosome Mapping Chromosomes Chromosomes, Bacterial - drug effects Culture Techniques DNA DNA Replication DNA, Bacterial - biosynthesis Escherichia coli - drug effects Escherichia coli - metabolism Genetic mutation Guanidines - pharmacology Mutation Nalidixic Acid - pharmacology Nitroso Compounds - pharmacology Salts Starvation Time Factors Washing |
| title | Synchronous Reinitiation of Chromosome Replication in E. coli B/r after Nalidixic Acid Treatment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T23%3A51%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Synchronous%20Reinitiation%20of%20Chromosome%20Replication%20in%20E.%20coli%20B/r%20after%20Nalidixic%20Acid%20Treatment&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Ward,%20C.%20B.&rft.date=1970-06-01&rft.volume=66&rft.issue=2&rft.spage=365&rft.epage=369&rft.pages=365-369&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.66.2.365&rft_dat=%3Cjstor_pubme%3E60063%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=84640365&rft_id=info:pmid/4915886&rft_jstor_id=60063&rfr_iscdi=true |