High Throughput Quantitative Colorimetric Microneutralization Assay for the Confirmation and Differentiation of West Nile Virus and St. Louis Encephalitis Virus
An automated colorimetric micro-neutralization assay (CmNt) was developed for confirmation and differentiation of West Nile Virus (WNV)-positive human sera as a higher throughput alternative to the standard six-well plaque-reduction neutralization test (PRNT). CmNt was performed in high-capacity 96-...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2010-03, Vol.82 (3), p.501-504 |
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| container_title | The American journal of tropical medicine and hygiene |
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| creator | Taketa-Graham, Michael Powell Pereira, Jaime L Baylis, Elizabeth Cossen, Cynthia Oceguera, Leopoldo Patiris, Peter Chiles, Robert Hanson, Carl V Forghani, BagHer |
| description | An automated colorimetric micro-neutralization assay (CmNt) was developed for confirmation and differentiation of West Nile Virus (WNV)-positive human sera as a higher throughput alternative to the standard six-well plaque-reduction neutralization test (PRNT). CmNt was performed in high-capacity 96-well micro-titer plates and required 4-6 days to complete. Inhibition of infection was determined by reduced neutral red-dye retention and conveniently recorded by a colorimetric plate reader. Human sera previously confirmed by PRNT as either negative (N = 52), WNV positive (N = 81), or St. Louis encephalitis virus positive (N = 12) were tested by CmNt; interpreted results were virtually identical to PRNT with a reduced turnaround time and higher throughput. Additionally, a handful of dengue virus positive and negative specimens (four each) were tested by CmNt; interpreted results were identical to PRNT. |
| doi_str_mv | 10.4269/ajtmh.2010.09-0297 |
| format | Article |
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| source | Electronic Journals Library; MEDLINE; PubMed (Medline); Alma/SFX Local Collection |
| subjects | Animals Cercopithecus aethiops Colorimetry - methods Encephalitis Virus, St. Louis - isolation & purification Neutralization Tests - methods Short Report Vero Cells West Nile virus - isolation & purification |
| title | High Throughput Quantitative Colorimetric Microneutralization Assay for the Confirmation and Differentiation of West Nile Virus and St. Louis Encephalitis Virus |
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