External quality assessment of the molecular diagnostics and genotyping of meticillin-resistant Staphylococcus aureus
Two multicentre external quality assessments (EQA) for the molecular detection and genotyping of meticillin-resistant Staphylococcus aureus (MRSA) were arranged. Firstly, 11 samples containing various amounts of inactivated MRSA strains, meticillin-susceptible S. aureus (MSSA), meticillin-resistant...
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| Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2010-03, Vol.29 (3), p.295-300 |
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| creator | te Witt, R van Belkum, A MacKay, W. G Wallace, P. S van Leeuwen, W. B |
| description | Two multicentre external quality assessments (EQA) for the molecular detection and genotyping of meticillin-resistant Staphylococcus aureus (MRSA) were arranged. Firstly, 11 samples containing various amounts of inactivated MRSA strains, meticillin-susceptible S. aureus (MSSA), meticillin-resistant coagulase-negative staphylococci (MRCoNS) or Escherichia coli were distributed to 82 laboratories. Samples containing 10² or 10³ MRSA cells were correctly scored in only 16 and 46% of the datasets returned, respectively. Two of the used MSSA strains contained an SCCmec cassette lacking the mecA gene. There was a marked difference in the percentage of correct results for these two MSSA strains (37 and 39%) compared to the MSSA strain lacking the SCCmec cassette (88%). Secondly, a panel for MRSA genotyping, consisting of ten samples (two identical, three genetically related and five unique strains) was distributed to 19 laboratories. Seventy-three percent of the datasets recorded all samples correctly. Most pulsed-field gel electrophoresis (PFGE) protocols proved to be suboptimal, resulting in inferior resolution in the higher or lower fragment regions. The performance of molecular diagnostics for MRSA shows no significant changes since our first EQA in 2006. The first molecular typing results are encouraging. Both assessments indicate that programme expansion is required and that major performance discrepancies continue to exist. |
| doi_str_mv | 10.1007/s10096-009-0856-8 |
| format | Article |
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Secondly, a panel for MRSA genotyping, consisting of ten samples (two identical, three genetically related and five unique strains) was distributed to 19 laboratories. Seventy-three percent of the datasets recorded all samples correctly. Most pulsed-field gel electrophoresis (PFGE) protocols proved to be suboptimal, resulting in inferior resolution in the higher or lower fragment regions. The performance of molecular diagnostics for MRSA shows no significant changes since our first EQA in 2006. The first molecular typing results are encouraging. Both assessments indicate that programme expansion is required and that major performance discrepancies continue to exist.</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/s10096-009-0856-8</identifier><identifier>PMID: 20082105</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Bacterial diseases ; Bacterial Proteins - genetics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; DNA, Bacterial - analysis ; E coli ; Electrophoresis, Gel, Pulsed-Field - methods ; Escherichia coli ; Genotype ; Human bacterial diseases ; Humans ; Infectious diseases ; Internal Medicine ; Medical Microbiology ; Medical sciences ; Methicillin-Resistant Staphylococcus aureus - genetics ; Molecular Diagnostic Techniques - methods ; Penicillin-Binding Proteins ; Quality Assurance, Health Care - methods ; Staphylococcal Infections - microbiology ; Staphylococcal infections, streptococcal infections, pneumococcal infections ; Staphylococcus aureus ; Staphylococcus aureus - genetics</subject><ispartof>European journal of clinical microbiology & infectious diseases, 2010-03, Vol.29 (3), p.295-300</ispartof><rights>The Author(s) 2010</rights><rights>2015 INIST-CNRS</rights><rights>Springer-Verlag 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-913143985722aa0e34cf65755288969071b5b5a91bc7c04e86c9fe535d3317803</citedby><cites>FETCH-LOGICAL-c554t-913143985722aa0e34cf65755288969071b5b5a91bc7c04e86c9fe535d3317803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10096-009-0856-8$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10096-009-0856-8$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22536404$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20082105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>te Witt, R</creatorcontrib><creatorcontrib>van Belkum, A</creatorcontrib><creatorcontrib>MacKay, W. G</creatorcontrib><creatorcontrib>Wallace, P. S</creatorcontrib><creatorcontrib>van Leeuwen, W. B</creatorcontrib><title>External quality assessment of the molecular diagnostics and genotyping of meticillin-resistant Staphylococcus aureus</title><title>European journal of clinical microbiology & infectious diseases</title><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><description>Two multicentre external quality assessments (EQA) for the molecular detection and genotyping of meticillin-resistant Staphylococcus aureus (MRSA) were arranged. Firstly, 11 samples containing various amounts of inactivated MRSA strains, meticillin-susceptible S. aureus (MSSA), meticillin-resistant coagulase-negative staphylococci (MRCoNS) or Escherichia coli were distributed to 82 laboratories. Samples containing 10² or 10³ MRSA cells were correctly scored in only 16 and 46% of the datasets returned, respectively. Two of the used MSSA strains contained an SCCmec cassette lacking the mecA gene. There was a marked difference in the percentage of correct results for these two MSSA strains (37 and 39%) compared to the MSSA strain lacking the SCCmec cassette (88%). Secondly, a panel for MRSA genotyping, consisting of ten samples (two identical, three genetically related and five unique strains) was distributed to 19 laboratories. Seventy-three percent of the datasets recorded all samples correctly. Most pulsed-field gel electrophoresis (PFGE) protocols proved to be suboptimal, resulting in inferior resolution in the higher or lower fragment regions. The performance of molecular diagnostics for MRSA shows no significant changes since our first EQA in 2006. The first molecular typing results are encouraging. Both assessments indicate that programme expansion is required and that major performance discrepancies continue to exist.</description><subject>Bacterial diseases</subject><subject>Bacterial Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>DNA, Bacterial - analysis</subject><subject>E coli</subject><subject>Electrophoresis, Gel, Pulsed-Field - methods</subject><subject>Escherichia coli</subject><subject>Genotype</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Internal Medicine</subject><subject>Medical Microbiology</subject><subject>Medical sciences</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Penicillin-Binding Proteins</subject><subject>Quality Assurance, Health Care - methods</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal infections, streptococcal infections, pneumococcal infections</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - genetics</subject><issn>0934-9723</issn><issn>1435-4373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUtv1DAUhSMEokPhB7CBCAmxCvgZ25tKqCoPqRKL0rXl8TgZV4499XUQ8-9xmKEFFrC5XtzvHN-j0zTPMXqLERLvoE7Vd3V0SPK-kw-aFWaUd4wK-rBZIUVZpwShJ80TgBtUNVKIx80JQUgSjPiqmS--F5ejCe3tbIIv-9YAOIDJxdKmoS1b104pODsHk9uNN2NMULyF1sRNO7qYyn7n47iwk6sLH4KPXXbgoZjqcVXMbrsPySZr56qas5vhafNoMAHcs-N72lx_uPh6_qm7_PLx8_n7y85yzkqnMK1xlOSCEGOQo8wOPRecEylVr5DAa77mRuG1FRYxJ3urBscp31CKhUT0tDk7-O7m9eQ2tobKJuhd9pPJe52M139uot_qMX3TRBImqawGb44GOd3ODoqePFgXgokuzaCllEhxov5PCko5Y-yn56u_yJs0LxWAJlgKVHtZIHyAbE4A2Q13R2Okl_L1oXxdh17K14vmxe9p7xS_2q7A6yNgwJowZBOth3uOcNozxCpHDhzUVRxdvr_wX7-_PIgGk7QZczW-viIIU4Ql6gnm9Ad3-dH6</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>te Witt, R</creator><creator>van Belkum, A</creator><creator>MacKay, W. 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G</au><au>Wallace, P. S</au><au>van Leeuwen, W. B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>External quality assessment of the molecular diagnostics and genotyping of meticillin-resistant Staphylococcus aureus</atitle><jtitle>European journal of clinical microbiology & infectious diseases</jtitle><stitle>Eur J Clin Microbiol Infect Dis</stitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><date>2010-03-01</date><risdate>2010</risdate><volume>29</volume><issue>3</issue><spage>295</spage><epage>300</epage><pages>295-300</pages><issn>0934-9723</issn><eissn>1435-4373</eissn><abstract>Two multicentre external quality assessments (EQA) for the molecular detection and genotyping of meticillin-resistant Staphylococcus aureus (MRSA) were arranged. Firstly, 11 samples containing various amounts of inactivated MRSA strains, meticillin-susceptible S. aureus (MSSA), meticillin-resistant coagulase-negative staphylococci (MRCoNS) or Escherichia coli were distributed to 82 laboratories. Samples containing 10² or 10³ MRSA cells were correctly scored in only 16 and 46% of the datasets returned, respectively. Two of the used MSSA strains contained an SCCmec cassette lacking the mecA gene. There was a marked difference in the percentage of correct results for these two MSSA strains (37 and 39%) compared to the MSSA strain lacking the SCCmec cassette (88%). Secondly, a panel for MRSA genotyping, consisting of ten samples (two identical, three genetically related and five unique strains) was distributed to 19 laboratories. Seventy-three percent of the datasets recorded all samples correctly. Most pulsed-field gel electrophoresis (PFGE) protocols proved to be suboptimal, resulting in inferior resolution in the higher or lower fragment regions. The performance of molecular diagnostics for MRSA shows no significant changes since our first EQA in 2006. The first molecular typing results are encouraging. Both assessments indicate that programme expansion is required and that major performance discrepancies continue to exist.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>20082105</pmid><doi>10.1007/s10096-009-0856-8</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | European journal of clinical microbiology & infectious diseases, 2010-03, Vol.29 (3), p.295-300 |
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| subjects | Bacterial diseases Bacterial Proteins - genetics Biological and medical sciences Biomedical and Life Sciences Biomedicine DNA, Bacterial - analysis E coli Electrophoresis, Gel, Pulsed-Field - methods Escherichia coli Genotype Human bacterial diseases Humans Infectious diseases Internal Medicine Medical Microbiology Medical sciences Methicillin-Resistant Staphylococcus aureus - genetics Molecular Diagnostic Techniques - methods Penicillin-Binding Proteins Quality Assurance, Health Care - methods Staphylococcal Infections - microbiology Staphylococcal infections, streptococcal infections, pneumococcal infections Staphylococcus aureus Staphylococcus aureus - genetics |
| title | External quality assessment of the molecular diagnostics and genotyping of meticillin-resistant Staphylococcus aureus |
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