Protein interaction platforms: visualization of interacting proteins in yeast

Here we describe the protein interaction platform assay, a method for identifying interacting proteins in Saccharomyces cerevisiae. This assay relies on the reovirus scaffolding protein μNS, which forms large focal inclusions in living cells. When a query protein is fused to μNS and potential intera...

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Veröffentlicht in:	Nature methods 2009-07, Vol.6 (7), p.500-502
Hauptverfasser:	Lesser, Cammie F, Schmitz, Alexa M, Morrison, Monica F, Agunwamba, Akochi O, Nibert, Max L
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - metabolism Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Brewer's yeast brief-communication Cellular biology Enzymatic analysis Fluorescence Genetic aspects Life Sciences Methods Microbiological assay Mutation Physiological aspects Plasmids - genetics Protein Interaction Mapping - methods Protein-protein interactions Proteins Proteomics Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Reovirus Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Salmonella typhimurium - genetics Salmonella typhimurium - pathogenicity Scientific method Shigella flexneri - genetics Shigella flexneri - pathogenicity Virulence - genetics Yeast Yeasts
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Zusammenfassung:	Here we describe the protein interaction platform assay, a method for identifying interacting proteins in Saccharomyces cerevisiae. This assay relies on the reovirus scaffolding protein μNS, which forms large focal inclusions in living cells. When a query protein is fused to μNS and potential interaction partners are fused to a fluorescent reporter, interactors can be identified by screening for yeast that display fluorescent foci.
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth.1337