Comparing The Cytotoxic Potential Of Withania somnifera Water And Methanol Extracts
The plant Withania somnifera (Linn.) (Solanacea) is a well-known herbal medicine used in many parts of the world. It has anti-inflammatory, antioxidant, and antitumor as well as neural protective properties. It seems as if the two most active withanolide components, namely withaferin A and withanoli...
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| Veröffentlicht in: | African journal of traditional, complementary, and alternative medicines complementary, and alternative medicines, 2009-05, Vol.6 (3), p.275-280 |
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| container_title | African journal of traditional, complementary, and alternative medicines |
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| creator | Pretorius, Etheresia Oberholzer, Hester Magdalena Becker, Petrus Johannes |
| description | The plant Withania somnifera (Linn.) (Solanacea) is a well-known
herbal medicine used in many parts of the world. It has
anti-inflammatory, antioxidant, and antitumor as well as neural
protective properties. It seems as if the two most active withanolide
components, namely withaferin A and withanolide D, found in methanol
(MeOH) extracts, are responsible for the anti-inflammatory and
antioxidant properties of the plant. The current research evaluated and
compared the cytotoxic potential of water and methanol extracts of W.
somnifera using a combined crystal violet MTT and Neutral Red assay.
MRC-5 cells, a human embryonic lung-derived diploid fibroblast cell
line, were the cells of choice. We found that the three lowest
concentrations (0.007, 0.042, 0.250 μg/ml) of the plant material
extracted in double distilled H2O and MeOH do not differ significantly
in any of the assays. We therefore suggest that low concentrations of
MeOH extracts (up to 0.250 μg/ml plant material) do not cause cell
damage to the MRC-5 cells, however, higher levels should be avoided as
cell viability and cell numbers are negatively influenced. |
| format | Article |
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herbal medicine used in many parts of the world. It has
anti-inflammatory, antioxidant, and antitumor as well as neural
protective properties. It seems as if the two most active withanolide
components, namely withaferin A and withanolide D, found in methanol
(MeOH) extracts, are responsible for the anti-inflammatory and
antioxidant properties of the plant. The current research evaluated and
compared the cytotoxic potential of water and methanol extracts of W.
somnifera using a combined crystal violet MTT and Neutral Red assay.
MRC-5 cells, a human embryonic lung-derived diploid fibroblast cell
line, were the cells of choice. We found that the three lowest
concentrations (0.007, 0.042, 0.250 μg/ml) of the plant material
extracted in double distilled H2O and MeOH do not differ significantly
in any of the assays. We therefore suggest that low concentrations of
MeOH extracts (up to 0.250 μg/ml plant material) do not cause cell
damage to the MRC-5 cells, however, higher levels should be avoided as
cell viability and cell numbers are negatively influenced.</description><identifier>ISSN: 0189-6016</identifier><identifier>EISSN: 2505-0044</identifier><identifier>EISSN: 0189-6016</identifier><identifier>PMID: 20448853</identifier><language>eng</language><publisher>Nigeria: African Ethnomedicines Network</publisher><subject>Animals ; Anti-Inflammatory Agents - toxicity ; Cells, Cultured - drug effects ; Dose-Response Relationship, Drug ; Formazans ; Gentian Violet ; Humans ; Methanol ; methanol extract ; Neutral Red ; Plant Extracts - pharmacology ; Plant Roots - chemistry ; Research Paper ; Tetrazolium Salts ; Water ; water extract ; Withania - chemistry ; Withania - toxicity ; Withania somnifera, anti-inflammatory</subject><ispartof>African journal of traditional, complementary, and alternative medicines, 2009-05, Vol.6 (3), p.275-280</ispartof><rights>Copyright 2009 - African Journal Traditional, Complementary and Alternative Medicines</rights><rights>Copyright © Afr. J. Traditional, Complementary and Alternative Medicines 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2816463/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2816463/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,53766,53768,79395</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20448853$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pretorius, Etheresia</creatorcontrib><creatorcontrib>Oberholzer, Hester Magdalena</creatorcontrib><creatorcontrib>Becker, Petrus Johannes</creatorcontrib><title>Comparing The Cytotoxic Potential Of Withania somnifera Water And Methanol Extracts</title><title>African journal of traditional, complementary, and alternative medicines</title><addtitle>Afr J Tradit Complement Altern Med</addtitle><description>The plant Withania somnifera (Linn.) (Solanacea) is a well-known
herbal medicine used in many parts of the world. It has
anti-inflammatory, antioxidant, and antitumor as well as neural
protective properties. It seems as if the two most active withanolide
components, namely withaferin A and withanolide D, found in methanol
(MeOH) extracts, are responsible for the anti-inflammatory and
antioxidant properties of the plant. The current research evaluated and
compared the cytotoxic potential of water and methanol extracts of W.
somnifera using a combined crystal violet MTT and Neutral Red assay.
MRC-5 cells, a human embryonic lung-derived diploid fibroblast cell
line, were the cells of choice. We found that the three lowest
concentrations (0.007, 0.042, 0.250 μg/ml) of the plant material
extracted in double distilled H2O and MeOH do not differ significantly
in any of the assays. We therefore suggest that low concentrations of
MeOH extracts (up to 0.250 μg/ml plant material) do not cause cell
damage to the MRC-5 cells, however, higher levels should be avoided as
cell viability and cell numbers are negatively influenced.</description><subject>Animals</subject><subject>Anti-Inflammatory Agents - toxicity</subject><subject>Cells, Cultured - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Formazans</subject><subject>Gentian Violet</subject><subject>Humans</subject><subject>Methanol</subject><subject>methanol extract</subject><subject>Neutral Red</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Roots - chemistry</subject><subject>Research Paper</subject><subject>Tetrazolium Salts</subject><subject>Water</subject><subject>water extract</subject><subject>Withania - chemistry</subject><subject>Withania - toxicity</subject><subject>Withania somnifera, anti-inflammatory</subject><issn>0189-6016</issn><issn>2505-0044</issn><issn>0189-6016</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><sourceid>EIF</sourceid><recordid>eNpVkOtKAzEQhYMotlZfQfICK7kn-0coS71ApYKV_lyy2Wwb2U1KNkr79q7UFoWBgTnfHM7MGRgTjniGEGPnYIywyjOBsBiBq77_QIgqTOQlGJFBV4rTMXgrQrfV0fk1XG4sLPYppLBzBr6GZH1yuoWLBq5c2mjvNOxD511jo4YrnWyEU1_DF_sjhhbOdilqk_prcNHotrc3v30C3h9my-Ipmy8en4vpPKsoYikj3EhuMM4RxdzmClOpqJBIiCavUI1rogirjKKGS0xsZRi3jWwkk9RUItd0Au4PvtvPqrO1GfJG3Zbb6Dod92XQrvyveLcp1-GrJAoLJuhgcPvX4LR5fM8A3B2AyoXWeXsiTHS6PA6TGQoNZ0j6DWmIdBw</recordid><startdate>20090507</startdate><enddate>20090507</enddate><creator>Pretorius, Etheresia</creator><creator>Oberholzer, Hester Magdalena</creator><creator>Becker, Petrus Johannes</creator><general>African Ethnomedicines Network</general><general>African Networks on Ethnomedicines</general><scope>RBI</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>20090507</creationdate><title>Comparing The Cytotoxic Potential Of Withania somnifera Water And Methanol Extracts</title><author>Pretorius, Etheresia ; Oberholzer, Hester Magdalena ; Becker, Petrus Johannes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b304t-25c75c1190315e981378367066f9b0d1d2824bc83c5712ebc45ef7f7473cb69a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents - toxicity</topic><topic>Cells, Cultured - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Formazans</topic><topic>Gentian Violet</topic><topic>Humans</topic><topic>Methanol</topic><topic>methanol extract</topic><topic>Neutral Red</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Roots - chemistry</topic><topic>Research Paper</topic><topic>Tetrazolium Salts</topic><topic>Water</topic><topic>water extract</topic><topic>Withania - chemistry</topic><topic>Withania - toxicity</topic><topic>Withania somnifera, anti-inflammatory</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pretorius, Etheresia</creatorcontrib><creatorcontrib>Oberholzer, Hester Magdalena</creatorcontrib><creatorcontrib>Becker, Petrus Johannes</creatorcontrib><collection>Bioline International</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>African journal of traditional, complementary, and alternative medicines</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pretorius, Etheresia</au><au>Oberholzer, Hester Magdalena</au><au>Becker, Petrus Johannes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparing The Cytotoxic Potential Of Withania somnifera Water And Methanol Extracts</atitle><jtitle>African journal of traditional, complementary, and alternative medicines</jtitle><addtitle>Afr J Tradit Complement Altern Med</addtitle><date>2009-05-07</date><risdate>2009</risdate><volume>6</volume><issue>3</issue><spage>275</spage><epage>280</epage><pages>275-280</pages><issn>0189-6016</issn><eissn>2505-0044</eissn><eissn>0189-6016</eissn><abstract>The plant Withania somnifera (Linn.) (Solanacea) is a well-known
herbal medicine used in many parts of the world. It has
anti-inflammatory, antioxidant, and antitumor as well as neural
protective properties. It seems as if the two most active withanolide
components, namely withaferin A and withanolide D, found in methanol
(MeOH) extracts, are responsible for the anti-inflammatory and
antioxidant properties of the plant. The current research evaluated and
compared the cytotoxic potential of water and methanol extracts of W.
somnifera using a combined crystal violet MTT and Neutral Red assay.
MRC-5 cells, a human embryonic lung-derived diploid fibroblast cell
line, were the cells of choice. We found that the three lowest
concentrations (0.007, 0.042, 0.250 μg/ml) of the plant material
extracted in double distilled H2O and MeOH do not differ significantly
in any of the assays. We therefore suggest that low concentrations of
MeOH extracts (up to 0.250 μg/ml plant material) do not cause cell
damage to the MRC-5 cells, however, higher levels should be avoided as
cell viability and cell numbers are negatively influenced.</abstract><cop>Nigeria</cop><pub>African Ethnomedicines Network</pub><pmid>20448853</pmid><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0189-6016 |
| ispartof | African journal of traditional, complementary, and alternative medicines, 2009-05, Vol.6 (3), p.275-280 |
| issn | 0189-6016 2505-0044 0189-6016 |
| language | eng |
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| source | MEDLINE; Bioline International; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Anti-Inflammatory Agents - toxicity Cells, Cultured - drug effects Dose-Response Relationship, Drug Formazans Gentian Violet Humans Methanol methanol extract Neutral Red Plant Extracts - pharmacology Plant Roots - chemistry Research Paper Tetrazolium Salts Water water extract Withania - chemistry Withania - toxicity Withania somnifera, anti-inflammatory |
| title | Comparing The Cytotoxic Potential Of Withania somnifera Water And Methanol Extracts |
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