Structure of an RNA Polymerase II-TFIIB Complex and the Transcription Initiation Mechanism
Previous x-ray crystal structures have given insight into the mechanism of transcription and the role of general transcription factors in the initiation of the process. A structure of an RNA polymerase II-general transcription factor TFIIB complex at 4.5 angstrom resolution revealed the amino-termin...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2010-01, Vol.327 (5962), p.206-209 |
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| description | Previous x-ray crystal structures have given insight into the mechanism of transcription and the role of general transcription factors in the initiation of the process. A structure of an RNA polymerase II-general transcription factor TFIIB complex at 4.5 angstrom resolution revealed the amino-terminal region of TFIIB, including a loop termed the "B finger," reaching into the active center of the polymerase where it may interact with both DNA and RNA, but this structure showed little of the carboxyl-terminal region. A new crystal structure of the same complex at 3.8 angstrom resolution obtained under different solution conditions is complementary with the previous one, revealing the carboxyl-terminal region of TFIIB, located above the polymerase active center cleft, but showing none of the B finger. In the new structure, the linker between the amino- and carboxyl-terminal regions can also be seen, snaking down from above the cleft toward the active center. The two structures, taken together with others previously obtained, dispel long-standing mysteries of the transcription initiation process. |
| doi_str_mv | 10.1126/science.1182015 |
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(ANL), Argonne, IL (United States). Advanced Photon Source (APS)</creatorcontrib><description>Previous x-ray crystal structures have given insight into the mechanism of transcription and the role of general transcription factors in the initiation of the process. A structure of an RNA polymerase II-general transcription factor TFIIB complex at 4.5 angstrom resolution revealed the amino-terminal region of TFIIB, including a loop termed the "B finger," reaching into the active center of the polymerase where it may interact with both DNA and RNA, but this structure showed little of the carboxyl-terminal region. A new crystal structure of the same complex at 3.8 angstrom resolution obtained under different solution conditions is complementary with the previous one, revealing the carboxyl-terminal region of TFIIB, located above the polymerase active center cleft, but showing none of the B finger. In the new structure, the linker between the amino- and carboxyl-terminal regions can also be seen, snaking down from above the cleft toward the active center. The two structures, taken together with others previously obtained, dispel long-standing mysteries of the transcription initiation process.</description><identifier>ISSN: 0036-8075</identifier><identifier>ISSN: 0193-4511</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1182015</identifier><identifier>PMID: 19965383</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Amino Acid Sequence ; Biochemistry ; Biological and medical sciences ; Catalytic Domain ; CRYSTAL STRUCTURE ; Crystalline structure ; Crystallography, X-Ray ; Cyclins ; DNA ; Electron density ; Fundamental and applied biological sciences. Psychology ; MATERIALS SCIENCE ; Models, Molecular ; Molecular biology ; Molecular biophysics ; Molecular Sequence Data ; POLYMERASES ; Protein Conformation ; Protein Interaction Domains and Motifs ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Repetitive Sequences, Amino Acid ; RESOLUTION ; RNA ; RNA polymerase ; RNA Polymerase II - chemistry ; RNA Polymerase II - metabolism ; RNA POLYMERASES ; Saccharomyces cerevisiae - chemistry ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - chemistry ; Saccharomyces cerevisiae Proteins - metabolism ; Structure in molecular biology ; TATA box ; TRANSCRIPTION ; Transcription Factor TFIIB - chemistry ; Transcription Factor TFIIB - metabolism ; TRANSCRIPTION FACTORS ; Transcription, Genetic ; Tunnels ; Yeasts ; Zinc</subject><ispartof>Science (American Association for the Advancement of Science), 2010-01, Vol.327 (5962), p.206-209</ispartof><rights>Copyright 2010 American Association for the Advancement of Science</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010, American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c680t-a386786f9fbbc0856141ad3fde57426cd3160a9e4e7441d96c76dc0484448a3b3</citedby><cites>FETCH-LOGICAL-c680t-a386786f9fbbc0856141ad3fde57426cd3160a9e4e7441d96c76dc0484448a3b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/40508820$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/40508820$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,2871,2872,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22288865$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19965383$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1002243$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Bushnell, David A</creatorcontrib><creatorcontrib>Wang, Dong</creatorcontrib><creatorcontrib>Calero, Guillermo</creatorcontrib><creatorcontrib>Kornberg, Roger D</creatorcontrib><creatorcontrib>Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)</creatorcontrib><title>Structure of an RNA Polymerase II-TFIIB Complex and the Transcription Initiation Mechanism</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Previous x-ray crystal structures have given insight into the mechanism of transcription and the role of general transcription factors in the initiation of the process. A structure of an RNA polymerase II-general transcription factor TFIIB complex at 4.5 angstrom resolution revealed the amino-terminal region of TFIIB, including a loop termed the "B finger," reaching into the active center of the polymerase where it may interact with both DNA and RNA, but this structure showed little of the carboxyl-terminal region. A new crystal structure of the same complex at 3.8 angstrom resolution obtained under different solution conditions is complementary with the previous one, revealing the carboxyl-terminal region of TFIIB, located above the polymerase active center cleft, but showing none of the B finger. In the new structure, the linker between the amino- and carboxyl-terminal regions can also be seen, snaking down from above the cleft toward the active center. The two structures, taken together with others previously obtained, dispel long-standing mysteries of the transcription initiation process.</description><subject>Amino Acid Sequence</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Catalytic Domain</subject><subject>CRYSTAL STRUCTURE</subject><subject>Crystalline structure</subject><subject>Crystallography, X-Ray</subject><subject>Cyclins</subject><subject>DNA</subject><subject>Electron density</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>MATERIALS SCIENCE</subject><subject>Models, Molecular</subject><subject>Molecular biology</subject><subject>Molecular biophysics</subject><subject>Molecular Sequence Data</subject><subject>POLYMERASES</subject><subject>Protein Conformation</subject><subject>Protein Interaction Domains and Motifs</subject><subject>Protein Structure, Secondary</subject><subject>Protein Structure, Tertiary</subject><subject>Repetitive Sequences, Amino Acid</subject><subject>RESOLUTION</subject><subject>RNA</subject><subject>RNA polymerase</subject><subject>RNA Polymerase II - chemistry</subject><subject>RNA Polymerase II - metabolism</subject><subject>RNA POLYMERASES</subject><subject>Saccharomyces cerevisiae - chemistry</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - chemistry</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Structure in molecular biology</subject><subject>TATA box</subject><subject>TRANSCRIPTION</subject><subject>Transcription Factor TFIIB - chemistry</subject><subject>Transcription Factor TFIIB - metabolism</subject><subject>TRANSCRIPTION FACTORS</subject><subject>Transcription, Genetic</subject><subject>Tunnels</subject><subject>Yeasts</subject><subject>Zinc</subject><issn>0036-8075</issn><issn>0193-4511</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1v1DAQxSMEokvhzAmIkAqnUNvjOM4FqawoRCofotsLF8vrOF2vEnuxHdT-93hJtIULnGzr_fxm_DxZ9hSjNxgTdhqU0VbpdOAE4fJetsCoLouaILifLRACVnBUlUfZoxC2CCWthofZEa5rVgKHRfb9MvpRxdHr3HW5tPm3z2f5V9ffDtrLoPOmKVbnTfMuX7ph1-ubhLR53Oh85aUNyptdNM7mjTXRyN_bT1ptpDVheJw96GQf9JN5Pc6uzt-vlh-Liy8fmuXZRaEYR7GQwFnFWVd367VCvGSYYtlC1-qyooSpFjBDstZUV5TitmaqYq1ClFNKuYQ1HGdvJ9_duB50q7SNXvZi580g_a1w0oi_FWs24tr9FIRjIKxKBi8nAxeiESnSmJ6gnLVaRYERIoRCgl7PVbz7MeoQxWCC0n0vrXZjEBVQAghXPJGv_kkCo0Aog_-CBAMmwOq7Bg_g1o3eplD3DENQViRBpxOkvAvB6-6QAEZiPyxiHhYxD0u68fzP4O74eToScDIDMijZd-nLlQkHjhDCOWd7o2cTtw3R-YNOUYl4KpX0F5PeSSfktU8eV5ephX1eBHDK4hdPC9m9</recordid><startdate>20100108</startdate><enddate>20100108</enddate><creator>Liu, Xin</creator><creator>Bushnell, David A</creator><creator>Wang, Dong</creator><creator>Calero, Guillermo</creator><creator>Kornberg, Roger D</creator><general>American Association for the Advancement of Science</general><general>The American Association for the Advancement of Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>20100108</creationdate><title>Structure of an RNA Polymerase II-TFIIB Complex and the Transcription Initiation Mechanism</title><author>Liu, Xin ; Bushnell, David A ; Wang, Dong ; Calero, Guillermo ; Kornberg, Roger D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c680t-a386786f9fbbc0856141ad3fde57426cd3160a9e4e7441d96c76dc0484448a3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino Acid Sequence</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Catalytic Domain</topic><topic>CRYSTAL STRUCTURE</topic><topic>Crystalline structure</topic><topic>Crystallography, X-Ray</topic><topic>Cyclins</topic><topic>DNA</topic><topic>Electron density</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>MATERIALS SCIENCE</topic><topic>Models, Molecular</topic><topic>Molecular biology</topic><topic>Molecular biophysics</topic><topic>Molecular Sequence Data</topic><topic>POLYMERASES</topic><topic>Protein Conformation</topic><topic>Protein Interaction Domains and Motifs</topic><topic>Protein Structure, Secondary</topic><topic>Protein Structure, Tertiary</topic><topic>Repetitive Sequences, Amino Acid</topic><topic>RESOLUTION</topic><topic>RNA</topic><topic>RNA polymerase</topic><topic>RNA Polymerase II - chemistry</topic><topic>RNA Polymerase II - metabolism</topic><topic>RNA POLYMERASES</topic><topic>Saccharomyces cerevisiae - chemistry</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - chemistry</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Structure in molecular biology</topic><topic>TATA box</topic><topic>TRANSCRIPTION</topic><topic>Transcription Factor TFIIB - chemistry</topic><topic>Transcription Factor TFIIB - metabolism</topic><topic>TRANSCRIPTION FACTORS</topic><topic>Transcription, Genetic</topic><topic>Tunnels</topic><topic>Yeasts</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Bushnell, David A</creatorcontrib><creatorcontrib>Wang, Dong</creatorcontrib><creatorcontrib>Calero, Guillermo</creatorcontrib><creatorcontrib>Kornberg, Roger D</creatorcontrib><creatorcontrib>Argonne National Lab. 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(ANL), Argonne, IL (United States). Advanced Photon Source (APS)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure of an RNA Polymerase II-TFIIB Complex and the Transcription Initiation Mechanism</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2010-01-08</date><risdate>2010</risdate><volume>327</volume><issue>5962</issue><spage>206</spage><epage>209</epage><pages>206-209</pages><issn>0036-8075</issn><issn>0193-4511</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Previous x-ray crystal structures have given insight into the mechanism of transcription and the role of general transcription factors in the initiation of the process. A structure of an RNA polymerase II-general transcription factor TFIIB complex at 4.5 angstrom resolution revealed the amino-terminal region of TFIIB, including a loop termed the "B finger," reaching into the active center of the polymerase where it may interact with both DNA and RNA, but this structure showed little of the carboxyl-terminal region. A new crystal structure of the same complex at 3.8 angstrom resolution obtained under different solution conditions is complementary with the previous one, revealing the carboxyl-terminal region of TFIIB, located above the polymerase active center cleft, but showing none of the B finger. In the new structure, the linker between the amino- and carboxyl-terminal regions can also be seen, snaking down from above the cleft toward the active center. The two structures, taken together with others previously obtained, dispel long-standing mysteries of the transcription initiation process.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>19965383</pmid><doi>10.1126/science.1182015</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Amino Acid Sequence Biochemistry Biological and medical sciences Catalytic Domain CRYSTAL STRUCTURE Crystalline structure Crystallography, X-Ray Cyclins DNA Electron density Fundamental and applied biological sciences. Psychology MATERIALS SCIENCE Models, Molecular Molecular biology Molecular biophysics Molecular Sequence Data POLYMERASES Protein Conformation Protein Interaction Domains and Motifs Protein Structure, Secondary Protein Structure, Tertiary Repetitive Sequences, Amino Acid RESOLUTION RNA RNA polymerase RNA Polymerase II - chemistry RNA Polymerase II - metabolism RNA POLYMERASES Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - metabolism Structure in molecular biology TATA box TRANSCRIPTION Transcription Factor TFIIB - chemistry Transcription Factor TFIIB - metabolism TRANSCRIPTION FACTORS Transcription, Genetic Tunnels Yeasts Zinc |
| title | Structure of an RNA Polymerase II-TFIIB Complex and the Transcription Initiation Mechanism |
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