Analysis of cooperativity by isothermal titration calorimetry
Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affiniti...
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description | Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples from the literature are used to demonstrate the applicability of ITC in the characterisation of cooperative systems. |
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This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples from the literature are used to demonstrate the applicability of ITC in the characterisation of cooperative systems.</description><subject>Archaeal Proteins - chemistry</subject><subject>Archaeal Proteins - metabolism</subject><subject>Calorimetry</subject><subject>DNA - chemistry</subject><subject>DNA - metabolism</subject><subject>DNA-Binding Proteins - chemistry</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Fatty Acid-Binding Proteins - chemistry</subject><subject>Fatty Acid-Binding Proteins - metabolism</subject><subject>Gastrointestinal Hormones - chemistry</subject><subject>Gastrointestinal Hormones - metabolism</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Ligands</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Protein Binding</subject><subject>Review</subject><subject>Thermodynamics</subject><subject>Thioredoxin-Disulfide Reductase - chemistry</subject><subject>Thioredoxin-Disulfide Reductase - metabolism</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc9LwzAcxYMobk5vnqXgwYvV_Gzag8IY_oKBFz2HJE1dRtvMJB30v7djc0xP3y-8D4_HewBcInhHSAHv7bIJCMKcUMaPwBhRjFMIM3588I_AWQhLCDHBrDgFIwwRQlnOx-Bh2sq6DzYkrkq0cyvjZbRrG_tE9YkNLi6Mb2SdRBs3imsTLWvnbWOi78_BSSXrYC52dwI-n58-Zq_p_P3lbTadp5pyHlONdMFpwZUiUFMDUYWVYliyMqNFqXFBGcYZ1LySlOVZrliBTKloDispodJkAh63vqtONabUph3C1GI1xJC-F05a8Vdp7UJ8ubXAOcI5gYPBzc7Au-_OhCgaG7Spa9ka1wXBCaEcsZwO5PU_cuk6P5QUBGJDe0WGBngCbreU9i4Eb6p9FgTFZhZxOMuAXx3m38O_O5AfZIyJ5A</recordid><startdate>20090804</startdate><enddate>20090804</enddate><creator>Brown, Alan</creator><general>MDPI AG</general><general>Molecular Diversity Preservation International (MDPI)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090804</creationdate><title>Analysis of cooperativity by isothermal titration calorimetry</title><author>Brown, Alan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-c1c97497bb30c4e01f2bb52a5d649dc29452260c7fa45868b591edb480faa0bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Archaeal Proteins - chemistry</topic><topic>Archaeal Proteins - metabolism</topic><topic>Calorimetry</topic><topic>DNA - chemistry</topic><topic>DNA - metabolism</topic><topic>DNA-Binding Proteins - chemistry</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Fatty Acid-Binding Proteins - chemistry</topic><topic>Fatty Acid-Binding Proteins - metabolism</topic><topic>Gastrointestinal Hormones - chemistry</topic><topic>Gastrointestinal Hormones - metabolism</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Ligands</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Protein Binding</topic><topic>Review</topic><topic>Thermodynamics</topic><topic>Thioredoxin-Disulfide Reductase - chemistry</topic><topic>Thioredoxin-Disulfide Reductase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, Alan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, Alan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of cooperativity by isothermal titration calorimetry</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2009-08-04</date><risdate>2009</risdate><volume>10</volume><issue>8</issue><spage>3457</spage><epage>3477</epage><pages>3457-3477</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Cooperative binding pervades Nature. 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subjects | Archaeal Proteins - chemistry Archaeal Proteins - metabolism Calorimetry DNA - chemistry DNA - metabolism DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism Fatty Acid-Binding Proteins - chemistry Fatty Acid-Binding Proteins - metabolism Gastrointestinal Hormones - chemistry Gastrointestinal Hormones - metabolism Humans Kinetics Ligands Magnetic Resonance Spectroscopy Protein Binding Review Thermodynamics Thioredoxin-Disulfide Reductase - chemistry Thioredoxin-Disulfide Reductase - metabolism |
title | Analysis of cooperativity by isothermal titration calorimetry |
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