Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia
Two Ag-ELISAs, an IgG-specific antibody detection ELISA (IgG ELISA) and a card agglutination test (CATT) for the detection of Trypanasoma evansi infections in buffaloes in Indonesia, were compared. Diagnostic sensitivity estimates were obtained by testing sera from 139 Indonesian buffaloes which had...
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| Veröffentlicht in: | Epidemiology and infection 1999-08, Vol.123 (1), p.149-155 |
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| creator | DAVISON, H. C. THRUSFIELD, M. V. MUHARSINI, S. HUSEIN, A. PARTOUTOMO, S. RAE, P. F. MASAKE, R. LUCKINS, A. G. |
| description | Two Ag-ELISAs, an IgG-specific antibody detection ELISA (IgG ELISA) and a card
agglutination test (CATT) for the detection of Trypanasoma evansi infections in buffaloes in
Indonesia, were compared. Diagnostic sensitivity estimates were obtained by testing sera from
139 Indonesian buffaloes which had been found to be infected by parasitological tests.
Diagnostic specificity was estimated by testing sera from 263 buffaloes living in Australia.
Response-operating characteristic curves were constructed, and optimal ELISA cut-off values,
which minimized the number of false–negative and false–positive results, were chosen. The IgG
ELISA had the highest sensitivity (89%) and the CATT had the highest specificity (100%).
There was a significant difference between the sensitivities (71 and 81%), but not between the
specificities (75 and 78%), of the two Ag-ELISAs. The four tests were further compared by
calculation of post-test probabilities of infection for positive and negative test results using a
range of prevalence values, and likelihood ratios. The results suggested that the CATT was the
best test to ‘rule-in’ infection (i.e. the highest probability of infection in test-positive animals)
and the IgG ELISA was the best test to ‘rule-out’ infection (i.e. the lowest probability of
infection in test-negative animals). |
| doi_str_mv | 10.1017/S0950268899002575 |
| format | Article |
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agglutination test (CATT) for the detection of Trypanasoma evansi infections in buffaloes in
Indonesia, were compared. Diagnostic sensitivity estimates were obtained by testing sera from
139 Indonesian buffaloes which had been found to be infected by parasitological tests.
Diagnostic specificity was estimated by testing sera from 263 buffaloes living in Australia.
Response-operating characteristic curves were constructed, and optimal ELISA cut-off values,
which minimized the number of false–negative and false–positive results, were chosen. The IgG
ELISA had the highest sensitivity (89%) and the CATT had the highest specificity (100%).
There was a significant difference between the sensitivities (71 and 81%), but not between the
specificities (75 and 78%), of the two Ag-ELISAs. The four tests were further compared by
calculation of post-test probabilities of infection for positive and negative test results using a
range of prevalence values, and likelihood ratios. The results suggested that the CATT was the
best test to ‘rule-in’ infection (i.e. the highest probability of infection in test-positive animals)
and the IgG ELISA was the best test to ‘rule-out’ infection (i.e. the lowest probability of
infection in test-negative animals).</description><identifier>ISSN: 0950-2688</identifier><identifier>EISSN: 1469-4409</identifier><identifier>DOI: 10.1017/S0950268899002575</identifier><identifier>PMID: 10487651</identifier><identifier>CODEN: EPINEU</identifier><language>eng</language><publisher>Cambridge: Cambridge University Press</publisher><subject>Agglutination Tests - veterinary ; Animal protozoal diseases ; Animals ; Antibodies ; Antibodies, Protozoan - blood ; Antigens ; Antigens, Protozoan - blood ; Biological and medical sciences ; Buffaloes ; Buffaloes - parasitology ; Enzyme linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - veterinary ; Fundamental and applied biological sciences. Psychology ; Indonesia - epidemiology ; Infections ; Infectious diseases ; Life cycle. Host-agent relationship. Pathogenesis ; Medical diagnostic tests ; Medical sciences ; Parasitic diseases ; Parasitism ; Point estimators ; Protozoa ; Protozoal diseases ; Sensitivity and Specificity ; Trypanosoma - immunology ; Trypanosoma - isolation & purification ; Trypanosoma evansi ; Trypanosome ; Trypanosomiasis - epidemiology ; Trypanosomiasis - veterinary ; Veterinary medicine</subject><ispartof>Epidemiology and infection, 1999-08, Vol.123 (1), p.149-155</ispartof><rights>1999 Cambridge University Press</rights><rights>Copyright 1999 Cambridge University Press</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c558t-e5e4d4d32b7e5dce7392c5b4c6ee645652a997c0558da0f07f0b7c4b9e8f85433</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4617454$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4617454$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1942266$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10487651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DAVISON, H. C.</creatorcontrib><creatorcontrib>THRUSFIELD, M. V.</creatorcontrib><creatorcontrib>MUHARSINI, S.</creatorcontrib><creatorcontrib>HUSEIN, A.</creatorcontrib><creatorcontrib>PARTOUTOMO, S.</creatorcontrib><creatorcontrib>RAE, P. F.</creatorcontrib><creatorcontrib>MASAKE, R.</creatorcontrib><creatorcontrib>LUCKINS, A. G.</creatorcontrib><title>Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia</title><title>Epidemiology and infection</title><addtitle>Epidemiol. Infect</addtitle><description>Two Ag-ELISAs, an IgG-specific antibody detection ELISA (IgG ELISA) and a card
agglutination test (CATT) for the detection of Trypanasoma evansi infections in buffaloes in
Indonesia, were compared. Diagnostic sensitivity estimates were obtained by testing sera from
139 Indonesian buffaloes which had been found to be infected by parasitological tests.
Diagnostic specificity was estimated by testing sera from 263 buffaloes living in Australia.
Response-operating characteristic curves were constructed, and optimal ELISA cut-off values,
which minimized the number of false–negative and false–positive results, were chosen. The IgG
ELISA had the highest sensitivity (89%) and the CATT had the highest specificity (100%).
There was a significant difference between the sensitivities (71 and 81%), but not between the
specificities (75 and 78%), of the two Ag-ELISAs. The four tests were further compared by
calculation of post-test probabilities of infection for positive and negative test results using a
range of prevalence values, and likelihood ratios. The results suggested that the CATT was the
best test to ‘rule-in’ infection (i.e. the highest probability of infection in test-positive animals)
and the IgG ELISA was the best test to ‘rule-out’ infection (i.e. the lowest probability of
infection in test-negative animals).</description><subject>Agglutination Tests - veterinary</subject><subject>Animal protozoal diseases</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Protozoan - blood</subject><subject>Antigens</subject><subject>Antigens, Protozoan - blood</subject><subject>Biological and medical sciences</subject><subject>Buffaloes</subject><subject>Buffaloes - parasitology</subject><subject>Enzyme linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Indonesia - epidemiology</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Medical diagnostic tests</subject><subject>Medical sciences</subject><subject>Parasitic diseases</subject><subject>Parasitism</subject><subject>Point estimators</subject><subject>Protozoa</subject><subject>Protozoal diseases</subject><subject>Sensitivity and Specificity</subject><subject>Trypanosoma - immunology</subject><subject>Trypanosoma - isolation & purification</subject><subject>Trypanosoma evansi</subject><subject>Trypanosome</subject><subject>Trypanosomiasis - epidemiology</subject><subject>Trypanosomiasis - veterinary</subject><subject>Veterinary medicine</subject><issn>0950-2688</issn><issn>1469-4409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9v0zAUxS0EYmXwAZAQygPiLWAn_hO_IME0ukkTqNp44cVynJviktidnVTrt8dtqlKEBE-Wzvn5-B5fhF4S_I5gIt7fYslwwatKSowLJtgjNCOUy5xSLB-j2c7Od_4ZehbjCmMsi0o8RWcE00pwRmbo4XKju1EP1rvMt5l2g12CyxoYwOxF7Zq9WvtmeyIPEIeYtT5kd2G71s5H3-sMNtpFm1nXTljcZdZj2-rOQ0x6du0a7yBa_Rw9SWqEF4fzHH37fHl3cZXffJ1fX3y8yQ1j1ZADA9rQpixqAawxIEpZGFZTwwE4ZZwVWkphcIIbjVssWlwLQ2sJVVsxWpbn6MOUux7rHlKCG4Lu1DrYXoet8tqqPx1nf6il36iiIliUVQp4ewgI_n5MtVVvo4Gu0w78GJVIXy9Jeup_IBElTWOyBJIJNMHHGKA9TkOw2i1W_bXYdOf1aY2TG9MmE_DmAOhodNcG7YyNvzlJi4LzhL2asFUcfDjalBNB9x3yybZxgIejrcNPxUUpmOLzhbpazMni9vsX9Snx5aGK7utgmyWolR-DSxv9R5lfIZLWSw</recordid><startdate>19990801</startdate><enddate>19990801</enddate><creator>DAVISON, H. C.</creator><creator>THRUSFIELD, M. V.</creator><creator>MUHARSINI, S.</creator><creator>HUSEIN, A.</creator><creator>PARTOUTOMO, S.</creator><creator>RAE, P. F.</creator><creator>MASAKE, R.</creator><creator>LUCKINS, A. G.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19990801</creationdate><title>Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia</title><author>DAVISON, H. C. ; THRUSFIELD, M. V. ; MUHARSINI, S. ; HUSEIN, A. ; PARTOUTOMO, S. ; RAE, P. F. ; MASAKE, R. ; LUCKINS, A. 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Pathogenesis</topic><topic>Medical diagnostic tests</topic><topic>Medical sciences</topic><topic>Parasitic diseases</topic><topic>Parasitism</topic><topic>Point estimators</topic><topic>Protozoa</topic><topic>Protozoal diseases</topic><topic>Sensitivity and Specificity</topic><topic>Trypanosoma - immunology</topic><topic>Trypanosoma - isolation & purification</topic><topic>Trypanosoma evansi</topic><topic>Trypanosome</topic><topic>Trypanosomiasis - epidemiology</topic><topic>Trypanosomiasis - veterinary</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DAVISON, H. C.</creatorcontrib><creatorcontrib>THRUSFIELD, M. V.</creatorcontrib><creatorcontrib>MUHARSINI, S.</creatorcontrib><creatorcontrib>HUSEIN, A.</creatorcontrib><creatorcontrib>PARTOUTOMO, S.</creatorcontrib><creatorcontrib>RAE, P. F.</creatorcontrib><creatorcontrib>MASAKE, R.</creatorcontrib><creatorcontrib>LUCKINS, A. G.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Epidemiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DAVISON, H. C.</au><au>THRUSFIELD, M. V.</au><au>MUHARSINI, S.</au><au>HUSEIN, A.</au><au>PARTOUTOMO, S.</au><au>RAE, P. F.</au><au>MASAKE, R.</au><au>LUCKINS, A. G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia</atitle><jtitle>Epidemiology and infection</jtitle><addtitle>Epidemiol. Infect</addtitle><date>1999-08-01</date><risdate>1999</risdate><volume>123</volume><issue>1</issue><spage>149</spage><epage>155</epage><pages>149-155</pages><issn>0950-2688</issn><eissn>1469-4409</eissn><coden>EPINEU</coden><abstract>Two Ag-ELISAs, an IgG-specific antibody detection ELISA (IgG ELISA) and a card
agglutination test (CATT) for the detection of Trypanasoma evansi infections in buffaloes in
Indonesia, were compared. Diagnostic sensitivity estimates were obtained by testing sera from
139 Indonesian buffaloes which had been found to be infected by parasitological tests.
Diagnostic specificity was estimated by testing sera from 263 buffaloes living in Australia.
Response-operating characteristic curves were constructed, and optimal ELISA cut-off values,
which minimized the number of false–negative and false–positive results, were chosen. The IgG
ELISA had the highest sensitivity (89%) and the CATT had the highest specificity (100%).
There was a significant difference between the sensitivities (71 and 81%), but not between the
specificities (75 and 78%), of the two Ag-ELISAs. The four tests were further compared by
calculation of post-test probabilities of infection for positive and negative test results using a
range of prevalence values, and likelihood ratios. The results suggested that the CATT was the
best test to ‘rule-in’ infection (i.e. the highest probability of infection in test-positive animals)
and the IgG ELISA was the best test to ‘rule-out’ infection (i.e. the lowest probability of
infection in test-negative animals).</abstract><cop>Cambridge</cop><pub>Cambridge University Press</pub><pmid>10487651</pmid><doi>10.1017/S0950268899002575</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Epidemiology and infection, 1999-08, Vol.123 (1), p.149-155 |
| issn | 0950-2688 1469-4409 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2810738 |
| source | MEDLINE; PubMed Central; JSTOR |
| subjects | Agglutination Tests - veterinary Animal protozoal diseases Animals Antibodies Antibodies, Protozoan - blood Antigens Antigens, Protozoan - blood Biological and medical sciences Buffaloes Buffaloes - parasitology Enzyme linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - veterinary Fundamental and applied biological sciences. Psychology Indonesia - epidemiology Infections Infectious diseases Life cycle. Host-agent relationship. Pathogenesis Medical diagnostic tests Medical sciences Parasitic diseases Parasitism Point estimators Protozoa Protozoal diseases Sensitivity and Specificity Trypanosoma - immunology Trypanosoma - isolation & purification Trypanosoma evansi Trypanosome Trypanosomiasis - epidemiology Trypanosomiasis - veterinary Veterinary medicine |
| title | Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia |
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