Drought, Abscisic Acid and Transpiration Rate Effects on the Regulation of PIP Aquaporin Gene Expression and Abundance in Phaseolus vulgaris Plants
BACKGROUND: and Aims Drought causes a decline of root hydraulic conductance, which aside from embolisms, is governed ultimately by aquaporins. Multiple factors probably regulate aquaporin expression, abundance and activity in leaf and root tissues during drought; among these are the leaf transpirati...
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| Veröffentlicht in: | Annals of botany 2006-12, Vol.98 (6), p.1301-1310 |
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| creator | AROCA, RICARDO FERRANTE, ANTONIO VERNIERI, PAOLO CHRISPEELS, MAARTEN J |
| description | BACKGROUND: and Aims Drought causes a decline of root hydraulic conductance, which aside from embolisms, is governed ultimately by aquaporins. Multiple factors probably regulate aquaporin expression, abundance and activity in leaf and root tissues during drought; among these are the leaf transpiration rate, leaf water status, abscisic acid (ABA) and soil water content. Here a study is made of how these factors could influence the response of aquaporin to drought. METHODS: Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants and their expression was analysed after 4 d of water deprivation and also 1 d after re-watering. The effects of ABA and of methotrexate (MTX), an inhibitor of stomatal opening, on gene expression and protein abundance were also analysed. Protein abundance was examined using antibodies against PIP1 and PIP2 aquaporins. At the same time, root hydraulic conductance (L), transpiration rate, leaf water status and ABA tissue concentration were measured. Key Results None of the treatments (drought, ABA or MTX) changed the leaf water status or tissue ABA concentration. The three treatments caused a decline in the transpiration rate and raised PVPIP2;1 gene expression and PIP1 protein abundance in the leaves. In the roots, only the drought treatment raised the expression of the three PIP genes examined, while at the same time diminishing PIP2 protein abundance and L. On the other hand, ABA raised both root PIP1 protein abundance and L. CONCLUSIONS: The rise of PvPIP2;1 gene expression and PIP1 protein abundance in the leaves of P. vulgaris plants subjected to drought was correlated with a decline in the transpiration rate. At the same time, the increase in the expression of the three PIP genes examined caused by drought and the decline of PIP2 protein abundance in the root tissues were not correlated with any of the parameters measured. |
| doi_str_mv | 10.1093/aob/mcl219 |
| format | Article |
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Multiple factors probably regulate aquaporin expression, abundance and activity in leaf and root tissues during drought; among these are the leaf transpiration rate, leaf water status, abscisic acid (ABA) and soil water content. Here a study is made of how these factors could influence the response of aquaporin to drought. METHODS: Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants and their expression was analysed after 4 d of water deprivation and also 1 d after re-watering. The effects of ABA and of methotrexate (MTX), an inhibitor of stomatal opening, on gene expression and protein abundance were also analysed. Protein abundance was examined using antibodies against PIP1 and PIP2 aquaporins. At the same time, root hydraulic conductance (L), transpiration rate, leaf water status and ABA tissue concentration were measured. Key Results None of the treatments (drought, ABA or MTX) changed the leaf water status or tissue ABA concentration. The three treatments caused a decline in the transpiration rate and raised PVPIP2;1 gene expression and PIP1 protein abundance in the leaves. In the roots, only the drought treatment raised the expression of the three PIP genes examined, while at the same time diminishing PIP2 protein abundance and L. On the other hand, ABA raised both root PIP1 protein abundance and L. CONCLUSIONS: The rise of PvPIP2;1 gene expression and PIP1 protein abundance in the leaves of P. vulgaris plants subjected to drought was correlated with a decline in the transpiration rate. At the same time, the increase in the expression of the three PIP genes examined caused by drought and the decline of PIP2 protein abundance in the root tissues were not correlated with any of the parameters measured.</description><identifier>ISSN: 0305-7364</identifier><identifier>EISSN: 1095-8290</identifier><identifier>DOI: 10.1093/aob/mcl219</identifier><identifier>PMID: 17028296</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Abscisic acid ; Abscisic Acid - pharmacology ; Antibodies ; Aquaporins ; Aquaporins - genetics ; Aquaporins - metabolism ; Cell membranes ; Desiccation ; Drought ; Gene expression regulation ; Gene Expression Regulation, Plant ; Hydraulics ; Leaves ; methotrexate ; Original ; Phaseolus - drug effects ; Phaseolus - genetics ; Phaseolus - metabolism ; Phaseolus vulgaris ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant roots ; Plant Transpiration - physiology ; Plants ; plasma membrane aquaporins ; root hydraulic conductance ; Transpiration ; transpiration rate</subject><ispartof>Annals of botany, 2006-12, Vol.98 (6), p.1301-1310</ispartof><rights>Annals of Botany Company 2006</rights><rights>Copyright Oxford University Press(England) Dec 15, 2006</rights><rights>The Author 2006. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-369ec09dd2a4bf31918d37539110ece2c74305a1087e357591d6306017a554e53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42801267$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42801267$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,728,781,785,804,886,27929,27930,53796,53798,58022,58255</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17028296$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>AROCA, RICARDO</creatorcontrib><creatorcontrib>FERRANTE, ANTONIO</creatorcontrib><creatorcontrib>VERNIERI, PAOLO</creatorcontrib><creatorcontrib>CHRISPEELS, MAARTEN J</creatorcontrib><title>Drought, Abscisic Acid and Transpiration Rate Effects on the Regulation of PIP Aquaporin Gene Expression and Abundance in Phaseolus vulgaris Plants</title><title>Annals of botany</title><addtitle>Ann Bot</addtitle><description>BACKGROUND: and Aims Drought causes a decline of root hydraulic conductance, which aside from embolisms, is governed ultimately by aquaporins. Multiple factors probably regulate aquaporin expression, abundance and activity in leaf and root tissues during drought; among these are the leaf transpiration rate, leaf water status, abscisic acid (ABA) and soil water content. Here a study is made of how these factors could influence the response of aquaporin to drought. METHODS: Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants and their expression was analysed after 4 d of water deprivation and also 1 d after re-watering. The effects of ABA and of methotrexate (MTX), an inhibitor of stomatal opening, on gene expression and protein abundance were also analysed. Protein abundance was examined using antibodies against PIP1 and PIP2 aquaporins. At the same time, root hydraulic conductance (L), transpiration rate, leaf water status and ABA tissue concentration were measured. Key Results None of the treatments (drought, ABA or MTX) changed the leaf water status or tissue ABA concentration. The three treatments caused a decline in the transpiration rate and raised PVPIP2;1 gene expression and PIP1 protein abundance in the leaves. In the roots, only the drought treatment raised the expression of the three PIP genes examined, while at the same time diminishing PIP2 protein abundance and L. On the other hand, ABA raised both root PIP1 protein abundance and L. CONCLUSIONS: The rise of PvPIP2;1 gene expression and PIP1 protein abundance in the leaves of P. vulgaris plants subjected to drought was correlated with a decline in the transpiration rate. At the same time, the increase in the expression of the three PIP genes examined caused by drought and the decline of PIP2 protein abundance in the root tissues were not correlated with any of the parameters measured.</description><subject>Abscisic acid</subject><subject>Abscisic Acid - pharmacology</subject><subject>Antibodies</subject><subject>Aquaporins</subject><subject>Aquaporins - genetics</subject><subject>Aquaporins - metabolism</subject><subject>Cell membranes</subject><subject>Desiccation</subject><subject>Drought</subject><subject>Gene expression regulation</subject><subject>Gene Expression Regulation, Plant</subject><subject>Hydraulics</subject><subject>Leaves</subject><subject>methotrexate</subject><subject>Original</subject><subject>Phaseolus - drug effects</subject><subject>Phaseolus - genetics</subject><subject>Phaseolus - metabolism</subject><subject>Phaseolus vulgaris</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant roots</subject><subject>Plant Transpiration - physiology</subject><subject>Plants</subject><subject>plasma membrane aquaporins</subject><subject>root hydraulic conductance</subject><subject>Transpiration</subject><subject>transpiration rate</subject><issn>0305-7364</issn><issn>1095-8290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1v0zAYxiMEYmVw4Q5YHDggwl7bcRJfkMrY1kmTqPYhoV0s13FSl9TubGcafwf_MK5SlY8LJ8t-fnr8PvaTZS8xfMTA6ZF0i6O16gnmj7JJOmF5TTg8ziZAgeUVLYuD7FkIKwAgJcdPswNcAUlMOcl-fvFu6JbxA5ougjLBKDRVpkHSNujaSxs2xstonEWXMmp00rZaxYDSPi41utTd0I-ya9H8fI6md4PcOG8sOtM28Q8br0PYAlvH6WKwjbRKowTMlzJo1w8B3Q99J70JaN5LG8Pz7Ekr-6Bf7NbD7Ob05Pp4ll98PTs_nl7kiuE65rTkWgFvGiKLRUsxx3VDK0Y5xqCVJqoqUn6Joa40ZRXjuCkplIAryVihGT3MPo2-m2Gx1o3SNnrZi403a-l_CCeN-FuxZik6dy9IDZTVZTJ4tzPw7m7QIYq1CUr3KYV2QxBljRkDoP8FMa8LTEmRwLf_gCs3eJteITEMY8z59tr3I6S8C8Hrdj8yBrFthEiNEGMjEvz6z5C_0V0FEvBqBFYhOr_XixQSk7JKej7qJkT9sNel_y6SWjEx-3Yr4PPsFIorIm4T_2bkW-mE7NK3ipsrAphCGr-EdOMvSVbUqg</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>AROCA, RICARDO</creator><creator>FERRANTE, ANTONIO</creator><creator>VERNIERI, PAOLO</creator><creator>CHRISPEELS, MAARTEN J</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7UA</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20061201</creationdate><title>Drought, Abscisic Acid and Transpiration Rate Effects on the Regulation of PIP Aquaporin Gene Expression and Abundance in Phaseolus vulgaris Plants</title><author>AROCA, RICARDO ; FERRANTE, ANTONIO ; VERNIERI, PAOLO ; CHRISPEELS, MAARTEN J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-369ec09dd2a4bf31918d37539110ece2c74305a1087e357591d6306017a554e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Abscisic acid</topic><topic>Abscisic Acid - pharmacology</topic><topic>Antibodies</topic><topic>Aquaporins</topic><topic>Aquaporins - genetics</topic><topic>Aquaporins - metabolism</topic><topic>Cell membranes</topic><topic>Desiccation</topic><topic>Drought</topic><topic>Gene expression regulation</topic><topic>Gene Expression Regulation, Plant</topic><topic>Hydraulics</topic><topic>Leaves</topic><topic>methotrexate</topic><topic>Original</topic><topic>Phaseolus - drug effects</topic><topic>Phaseolus - genetics</topic><topic>Phaseolus - metabolism</topic><topic>Phaseolus vulgaris</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant roots</topic><topic>Plant Transpiration - physiology</topic><topic>Plants</topic><topic>plasma membrane aquaporins</topic><topic>root hydraulic conductance</topic><topic>Transpiration</topic><topic>transpiration rate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AROCA, RICARDO</creatorcontrib><creatorcontrib>FERRANTE, ANTONIO</creatorcontrib><creatorcontrib>VERNIERI, PAOLO</creatorcontrib><creatorcontrib>CHRISPEELS, MAARTEN J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Water Resources Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Annals of botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AROCA, RICARDO</au><au>FERRANTE, ANTONIO</au><au>VERNIERI, PAOLO</au><au>CHRISPEELS, MAARTEN J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Drought, Abscisic Acid and Transpiration Rate Effects on the Regulation of PIP Aquaporin Gene Expression and Abundance in Phaseolus vulgaris Plants</atitle><jtitle>Annals of botany</jtitle><addtitle>Ann Bot</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>98</volume><issue>6</issue><spage>1301</spage><epage>1310</epage><pages>1301-1310</pages><issn>0305-7364</issn><eissn>1095-8290</eissn><abstract>BACKGROUND: and Aims Drought causes a decline of root hydraulic conductance, which aside from embolisms, is governed ultimately by aquaporins. Multiple factors probably regulate aquaporin expression, abundance and activity in leaf and root tissues during drought; among these are the leaf transpiration rate, leaf water status, abscisic acid (ABA) and soil water content. Here a study is made of how these factors could influence the response of aquaporin to drought. METHODS: Three plasma membrane intrinsic proteins (PIPs) or aquaporins were cloned from Phaseolus vulgaris plants and their expression was analysed after 4 d of water deprivation and also 1 d after re-watering. The effects of ABA and of methotrexate (MTX), an inhibitor of stomatal opening, on gene expression and protein abundance were also analysed. Protein abundance was examined using antibodies against PIP1 and PIP2 aquaporins. At the same time, root hydraulic conductance (L), transpiration rate, leaf water status and ABA tissue concentration were measured. Key Results None of the treatments (drought, ABA or MTX) changed the leaf water status or tissue ABA concentration. The three treatments caused a decline in the transpiration rate and raised PVPIP2;1 gene expression and PIP1 protein abundance in the leaves. In the roots, only the drought treatment raised the expression of the three PIP genes examined, while at the same time diminishing PIP2 protein abundance and L. On the other hand, ABA raised both root PIP1 protein abundance and L. CONCLUSIONS: The rise of PvPIP2;1 gene expression and PIP1 protein abundance in the leaves of P. vulgaris plants subjected to drought was correlated with a decline in the transpiration rate. At the same time, the increase in the expression of the three PIP genes examined caused by drought and the decline of PIP2 protein abundance in the root tissues were not correlated with any of the parameters measured.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>17028296</pmid><doi>10.1093/aob/mcl219</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Abscisic acid Abscisic Acid - pharmacology Antibodies Aquaporins Aquaporins - genetics Aquaporins - metabolism Cell membranes Desiccation Drought Gene expression regulation Gene Expression Regulation, Plant Hydraulics Leaves methotrexate Original Phaseolus - drug effects Phaseolus - genetics Phaseolus - metabolism Phaseolus vulgaris Plant Proteins - genetics Plant Proteins - metabolism Plant roots Plant Transpiration - physiology Plants plasma membrane aquaporins root hydraulic conductance Transpiration transpiration rate |
| title | Drought, Abscisic Acid and Transpiration Rate Effects on the Regulation of PIP Aquaporin Gene Expression and Abundance in Phaseolus vulgaris Plants |
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