Histone H2A.Z Regulates the Expression of Several Classes of Phosphate Starvation Response Genes But Not as a Transcriptional Activator
Phosphate (Pi) availability is a major constraint to plant growth. Consequently, plants have evolved complex adaptations to tolerate low Pi conditions. Numerous genes implicated in these adaptations have been identified, but their chromatin-level regulation has not been investigated. The nuclear act...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2010, Vol.152 (1), p.217-225 |
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| creator | Smith, Aaron P Jain, Ajay Deal, Roger B Nagarajan, Vinay K Poling, Michael D Raghothama, Kashchandra G Meagher, Richard B |
| description | Phosphate (Pi) availability is a major constraint to plant growth. Consequently, plants have evolved complex adaptations to tolerate low Pi conditions. Numerous genes implicated in these adaptations have been identified, but their chromatin-level regulation has not been investigated. The nuclear actin-related protein ARP6 is conserved among all eukaryotes and is an essential component of the SWR1 chromatin remodeling complex, which regulates transcription via deposition of the H2A.Z histone variant into chromatin. Here, we demonstrate that ARP6 is required for proper H2A.Z deposition at a number of Pi starvation response (PSR) genes in Arabidopsis (Arabidopsis thaliana). The loss of H2A.Z at these target loci results in their derepression in arp6 mutants and correlates with the presence of multiple Pi-starvation-related phenotypes, including shortened primary roots and increases in the number and length of root hairs, as well as increased starch accumulation and phosphatase activity in shoots. Our data suggest a model for chromatin-level control of Pi starvation responses in which ARP6-dependent H2A.Z deposition modulates the transcription of a suite of PSR genes. |
| doi_str_mv | 10.1104/pp.109.145532 |
| format | Article |
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Consequently, plants have evolved complex adaptations to tolerate low Pi conditions. Numerous genes implicated in these adaptations have been identified, but their chromatin-level regulation has not been investigated. The nuclear actin-related protein ARP6 is conserved among all eukaryotes and is an essential component of the SWR1 chromatin remodeling complex, which regulates transcription via deposition of the H2A.Z histone variant into chromatin. Here, we demonstrate that ARP6 is required for proper H2A.Z deposition at a number of Pi starvation response (PSR) genes in Arabidopsis (Arabidopsis thaliana). The loss of H2A.Z at these target loci results in their derepression in arp6 mutants and correlates with the presence of multiple Pi-starvation-related phenotypes, including shortened primary roots and increases in the number and length of root hairs, as well as increased starch accumulation and phosphatase activity in shoots. Our data suggest a model for chromatin-level control of Pi starvation responses in which ARP6-dependent H2A.Z deposition modulates the transcription of a suite of PSR genes.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.109.145532</identifier><identifier>PMID: 19897606</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Biologists</publisher><subject>Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Biological and medical sciences ; Chromatin ; ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS ; Fundamental and applied biological sciences. Psychology ; Gene expression regulation ; Gene Expression Regulation, Plant - physiology ; Genes ; Genetic loci ; Histones ; Histones - genetics ; Histones - metabolism ; Illicium ; Microfilament Proteins - genetics ; Microfilament Proteins - metabolism ; Mutation ; Phosphates ; Phosphoric Monoester Hydrolases - metabolism ; Plant physiology and development ; Plant Roots - growth & development ; Plant Roots - metabolism ; Plant Shoots - growth & development ; Plant Shoots - metabolism ; Plants ; Seedlings ; Starvation ; Yeasts</subject><ispartof>Plant physiology (Bethesda), 2010, Vol.152 (1), p.217-225</ispartof><rights>2010 American Society of Plant Biologists</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010, American Society of Plant Biologists 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-dc296cd253d5375974617c272fecefa256456f810e5b4949b61332b2c1a89e6f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25680642$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25680642$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,4010,27900,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22452585$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19897606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, Aaron P</creatorcontrib><creatorcontrib>Jain, Ajay</creatorcontrib><creatorcontrib>Deal, Roger B</creatorcontrib><creatorcontrib>Nagarajan, Vinay K</creatorcontrib><creatorcontrib>Poling, Michael D</creatorcontrib><creatorcontrib>Raghothama, Kashchandra G</creatorcontrib><creatorcontrib>Meagher, Richard B</creatorcontrib><title>Histone H2A.Z Regulates the Expression of Several Classes of Phosphate Starvation Response Genes But Not as a Transcriptional Activator</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Phosphate (Pi) availability is a major constraint to plant growth. Consequently, plants have evolved complex adaptations to tolerate low Pi conditions. Numerous genes implicated in these adaptations have been identified, but their chromatin-level regulation has not been investigated. The nuclear actin-related protein ARP6 is conserved among all eukaryotes and is an essential component of the SWR1 chromatin remodeling complex, which regulates transcription via deposition of the H2A.Z histone variant into chromatin. Here, we demonstrate that ARP6 is required for proper H2A.Z deposition at a number of Pi starvation response (PSR) genes in Arabidopsis (Arabidopsis thaliana). The loss of H2A.Z at these target loci results in their derepression in arp6 mutants and correlates with the presence of multiple Pi-starvation-related phenotypes, including shortened primary roots and increases in the number and length of root hairs, as well as increased starch accumulation and phosphatase activity in shoots. Our data suggest a model for chromatin-level control of Pi starvation responses in which ARP6-dependent H2A.Z deposition modulates the transcription of a suite of PSR genes.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biological and medical sciences</subject><subject>Chromatin</subject><subject>ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression regulation</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>Genes</subject><subject>Genetic loci</subject><subject>Histones</subject><subject>Histones - genetics</subject><subject>Histones - metabolism</subject><subject>Illicium</subject><subject>Microfilament Proteins - genetics</subject><subject>Microfilament Proteins - metabolism</subject><subject>Mutation</subject><subject>Phosphates</subject><subject>Phosphoric Monoester Hydrolases - metabolism</subject><subject>Plant physiology and development</subject><subject>Plant Roots - growth & development</subject><subject>Plant Roots - metabolism</subject><subject>Plant Shoots - growth & development</subject><subject>Plant Shoots - metabolism</subject><subject>Plants</subject><subject>Seedlings</subject><subject>Starvation</subject><subject>Yeasts</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVksFuEzEQhlcIREPhyBHwBXFKsL22d32pFKLSIFWAmvbCxXKc2WSrzXrxOBE8Aa_NRIlaOM3I8_mfGf8uiteCT4Tg6uMwTAS3E6G0LuWTYiQojKVW9dNixDnlvK7tWfEC8Z5zLkqhnhdnwta2MtyMij_zFnPsgc3ldPKD3cB61_kMyPIG2OWvIQFiG3sWG7aAPSTfsVnnEYmgo--biMOGeLbIPu19PqA3gEPsEdgV9IR92mX2NWbmkXl2m3yPIbXDgSStacgtXYvpZfGs8R3Cq1M8L-4-X97O5uPrb1dfZtPrcVC2zuNVkNaEldTlSpeVtpUyogqykg0EaLzURmnT1IKDXiqr7NKIspRLGYSvLZimPC8ujrrDbrmFVYA-005uSO3Wp98u-tb9X-nbjVvHvZOVtaWuSeDDSSDFnzvA7LYtBug630PcoavK0pAv2hI5PpIhRcQEzUMXwd3BOzcMlFp39I74t_-O9kifzCLg_QnwGHzX0FuGFh84KZWWutbEvTly92RteqxrU3OjDo3eHeuNj86vE2ncLST9DS4qyTUt-Rfs1LZy</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Smith, Aaron P</creator><creator>Jain, Ajay</creator><creator>Deal, Roger B</creator><creator>Nagarajan, Vinay K</creator><creator>Poling, Michael D</creator><creator>Raghothama, Kashchandra G</creator><creator>Meagher, Richard B</creator><general>American Society of Plant Biologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2010</creationdate><title>Histone H2A.Z Regulates the Expression of Several Classes of Phosphate Starvation Response Genes But Not as a Transcriptional Activator</title><author>Smith, Aaron P ; Jain, Ajay ; Deal, Roger B ; Nagarajan, Vinay K ; Poling, Michael D ; Raghothama, Kashchandra G ; Meagher, Richard B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-dc296cd253d5375974617c272fecefa256456f810e5b4949b61332b2c1a89e6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biological and medical sciences</topic><topic>Chromatin</topic><topic>ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression regulation</topic><topic>Gene Expression Regulation, Plant - physiology</topic><topic>Genes</topic><topic>Genetic loci</topic><topic>Histones</topic><topic>Histones - genetics</topic><topic>Histones - metabolism</topic><topic>Illicium</topic><topic>Microfilament Proteins - genetics</topic><topic>Microfilament Proteins - metabolism</topic><topic>Mutation</topic><topic>Phosphates</topic><topic>Phosphoric Monoester Hydrolases - metabolism</topic><topic>Plant physiology and development</topic><topic>Plant Roots - growth & development</topic><topic>Plant Roots - metabolism</topic><topic>Plant Shoots - growth & development</topic><topic>Plant Shoots - metabolism</topic><topic>Plants</topic><topic>Seedlings</topic><topic>Starvation</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, Aaron P</creatorcontrib><creatorcontrib>Jain, Ajay</creatorcontrib><creatorcontrib>Deal, Roger B</creatorcontrib><creatorcontrib>Nagarajan, Vinay K</creatorcontrib><creatorcontrib>Poling, Michael D</creatorcontrib><creatorcontrib>Raghothama, Kashchandra G</creatorcontrib><creatorcontrib>Meagher, Richard B</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Aaron P</au><au>Jain, Ajay</au><au>Deal, Roger B</au><au>Nagarajan, Vinay K</au><au>Poling, Michael D</au><au>Raghothama, Kashchandra G</au><au>Meagher, Richard B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histone H2A.Z Regulates the Expression of Several Classes of Phosphate Starvation Response Genes But Not as a Transcriptional Activator</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2010</date><risdate>2010</risdate><volume>152</volume><issue>1</issue><spage>217</spage><epage>225</epage><pages>217-225</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Phosphate (Pi) availability is a major constraint to plant growth. Consequently, plants have evolved complex adaptations to tolerate low Pi conditions. Numerous genes implicated in these adaptations have been identified, but their chromatin-level regulation has not been investigated. The nuclear actin-related protein ARP6 is conserved among all eukaryotes and is an essential component of the SWR1 chromatin remodeling complex, which regulates transcription via deposition of the H2A.Z histone variant into chromatin. Here, we demonstrate that ARP6 is required for proper H2A.Z deposition at a number of Pi starvation response (PSR) genes in Arabidopsis (Arabidopsis thaliana). The loss of H2A.Z at these target loci results in their derepression in arp6 mutants and correlates with the presence of multiple Pi-starvation-related phenotypes, including shortened primary roots and increases in the number and length of root hairs, as well as increased starch accumulation and phosphatase activity in shoots. 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| language | eng |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Biological and medical sciences Chromatin ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS Fundamental and applied biological sciences. Psychology Gene expression regulation Gene Expression Regulation, Plant - physiology Genes Genetic loci Histones Histones - genetics Histones - metabolism Illicium Microfilament Proteins - genetics Microfilament Proteins - metabolism Mutation Phosphates Phosphoric Monoester Hydrolases - metabolism Plant physiology and development Plant Roots - growth & development Plant Roots - metabolism Plant Shoots - growth & development Plant Shoots - metabolism Plants Seedlings Starvation Yeasts |
| title | Histone H2A.Z Regulates the Expression of Several Classes of Phosphate Starvation Response Genes But Not as a Transcriptional Activator |
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