Introducing a true internal standard for the Comet assay to minimize intra- and inter-experiment variability in measures of DNA damage and repair

The Comet assay (CA) is a sensitive/simple measure of genotoxicity. However, many features of CA contribute variability. To minimize these, we have introduced internal standard materials consisting of 'reference' cells which have their DNA substituted with BrdU. Using a fluorescent anti-Br...

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Veröffentlicht in:	Nucleic acids research 2009-12, Vol.37 (22), p.e150-e150
Hauptverfasser:	Zainol, Murizal, Stoute, Julia, Almeida, Gabriela M, Rapp, Alexander, Bowman, Karen J, Jones, George D.D
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line, Tumor Comet Assay - standards Data Interpretation, Statistical DNA Damage DNA Repair Humans Methods Online Reference Standards Reproducibility of Results
Online-Zugang:	Volltext
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