Actomyosin Is the Main Driver of Interkinetic Nuclear Migration in the Retina
Progenitor cell nuclei in the rapidly expanding epithelium of the embryonic vertebrate central nervous system undergo a process called interkinetic nuclear migration (IKNM). Movements of IKNM are generally believed to involve smooth migration of nuclei from apical to basal and back during the G1 and...
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| Veröffentlicht in: | Cell 2009-09, Vol.138 (6), p.1195-1208 |
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| creator | Norden, Caren Young, Stephen Link, Brian A. Harris, William A. |
| description | Progenitor cell nuclei in the rapidly expanding epithelium of the embryonic vertebrate central nervous system undergo a process called interkinetic nuclear migration (IKNM). Movements of IKNM are generally believed to involve smooth migration of nuclei from apical to basal and back during the G1 and G2 phases of the cell cycle, respectively. Yet, this has not been formally demonstrated, nor have the molecular mechanisms that drive IKNM been identified. Using time-lapse confocal microscopy to observe nuclear movements in zebrafish retinal neuroepithelial cells, we show that, except for brief apical nuclear translocations preceding mitosis, IKNM is stochastic rather than smooth and directed. We also show that IKNM is driven largely by actomyosin-dependent forces as it still occurs when the microtubule cytoskeleton is compromised but is blocked when MyosinII activity is inhibited. |
| doi_str_mv | 10.1016/j.cell.2009.06.032 |
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Movements of IKNM are generally believed to involve smooth migration of nuclei from apical to basal and back during the G1 and G2 phases of the cell cycle, respectively. Yet, this has not been formally demonstrated, nor have the molecular mechanisms that drive IKNM been identified. Using time-lapse confocal microscopy to observe nuclear movements in zebrafish retinal neuroepithelial cells, we show that, except for brief apical nuclear translocations preceding mitosis, IKNM is stochastic rather than smooth and directed. We also show that IKNM is driven largely by actomyosin-dependent forces as it still occurs when the microtubule cytoskeleton is compromised but is blocked when MyosinII activity is inhibited.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/j.cell.2009.06.032</identifier><identifier>PMID: 19766571</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Actomyosin - metabolism ; Animals ; Cell Nucleus - metabolism ; CELLBIO ; Danio rerio ; Dynactin Complex ; Embryo, Nonmammalian - cytology ; Embryo, Nonmammalian - metabolism ; Microtubule-Associated Proteins - metabolism ; MOLNEURO ; Neuroepithelial Cells - cytology ; Neuroepithelial Cells - metabolism ; Retina - cytology ; Retina - embryology ; Zebrafish - embryology ; Zebrafish - metabolism ; Zebrafish Proteins - metabolism</subject><ispartof>Cell, 2009-09, Vol.138 (6), p.1195-1208</ispartof><rights>2009 Elsevier Inc.</rights><rights>2009 ELL & Excerpta Medica. 2009 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-dda5fe3c8acf7aeb1e921d1edfefa21a7de61b86a0d8b911aef59c2314f53b573</citedby><cites>FETCH-LOGICAL-c550t-dda5fe3c8acf7aeb1e921d1edfefa21a7de61b86a0d8b911aef59c2314f53b573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S009286740900779X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19766571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Norden, Caren</creatorcontrib><creatorcontrib>Young, Stephen</creatorcontrib><creatorcontrib>Link, Brian A.</creatorcontrib><creatorcontrib>Harris, William A.</creatorcontrib><title>Actomyosin Is the Main Driver of Interkinetic Nuclear Migration in the Retina</title><title>Cell</title><addtitle>Cell</addtitle><description>Progenitor cell nuclei in the rapidly expanding epithelium of the embryonic vertebrate central nervous system undergo a process called interkinetic nuclear migration (IKNM). Movements of IKNM are generally believed to involve smooth migration of nuclei from apical to basal and back during the G1 and G2 phases of the cell cycle, respectively. Yet, this has not been formally demonstrated, nor have the molecular mechanisms that drive IKNM been identified. Using time-lapse confocal microscopy to observe nuclear movements in zebrafish retinal neuroepithelial cells, we show that, except for brief apical nuclear translocations preceding mitosis, IKNM is stochastic rather than smooth and directed. We also show that IKNM is driven largely by actomyosin-dependent forces as it still occurs when the microtubule cytoskeleton is compromised but is blocked when MyosinII activity is inhibited.</description><subject>Actomyosin - metabolism</subject><subject>Animals</subject><subject>Cell Nucleus - metabolism</subject><subject>CELLBIO</subject><subject>Danio rerio</subject><subject>Dynactin Complex</subject><subject>Embryo, Nonmammalian - cytology</subject><subject>Embryo, Nonmammalian - metabolism</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>MOLNEURO</subject><subject>Neuroepithelial Cells - cytology</subject><subject>Neuroepithelial Cells - metabolism</subject><subject>Retina - cytology</subject><subject>Retina - embryology</subject><subject>Zebrafish - embryology</subject><subject>Zebrafish - metabolism</subject><subject>Zebrafish Proteins - metabolism</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFDEYhYModlv9A17IXHk3Y97MJpkBEUq1daFbQfQ6ZJI3bdbZSU2yC_33zbCLHzf2KoHznENyDiFvgDZAQbzfNAbHsWGU9g0VDW3ZM7IA2st6CZI9J4sisLoTcnlCTlPaUEo7zvlLcgK9FIJLWJD1uclh-xCSn6pVqvIdVmtd7p-i32OsgqtWU8b400-YvaludmZEHau1v406-zBVhZ1N34o86VfkhdNjwtfH84z8uPz8_eJLff31anVxfl0bzmmurdXcYWs6bZzUOAD2DCygdeg0Ay0tChg6oanthh5Ao-O9YS0sHW8HLtsz8vGQe78btmgNTjnqUd1Hv9XxQQXt1b_K5O_UbdgrJnvo5Bzw7hgQw68dpqy2Ps1t6gnDLikhSz2yb58EGbC2AzGD7ACaGFKK6H6_Bqia51IbNfvUPJeiQpW5iunt3__4YznuU4APBwBLm3uPUSXjcTJofUSTlQ3-f_mPZTCowA</recordid><startdate>20090918</startdate><enddate>20090918</enddate><creator>Norden, Caren</creator><creator>Young, Stephen</creator><creator>Link, Brian A.</creator><creator>Harris, William A.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090918</creationdate><title>Actomyosin Is the Main Driver of Interkinetic Nuclear Migration in the Retina</title><author>Norden, Caren ; Young, Stephen ; Link, Brian A. ; Harris, William A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-dda5fe3c8acf7aeb1e921d1edfefa21a7de61b86a0d8b911aef59c2314f53b573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Actomyosin - metabolism</topic><topic>Animals</topic><topic>Cell Nucleus - metabolism</topic><topic>CELLBIO</topic><topic>Danio rerio</topic><topic>Dynactin Complex</topic><topic>Embryo, Nonmammalian - cytology</topic><topic>Embryo, Nonmammalian - metabolism</topic><topic>Microtubule-Associated Proteins - metabolism</topic><topic>MOLNEURO</topic><topic>Neuroepithelial Cells - cytology</topic><topic>Neuroepithelial Cells - metabolism</topic><topic>Retina - cytology</topic><topic>Retina - embryology</topic><topic>Zebrafish - embryology</topic><topic>Zebrafish - metabolism</topic><topic>Zebrafish Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Norden, Caren</creatorcontrib><creatorcontrib>Young, Stephen</creatorcontrib><creatorcontrib>Link, Brian A.</creatorcontrib><creatorcontrib>Harris, William A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Norden, Caren</au><au>Young, Stephen</au><au>Link, Brian A.</au><au>Harris, William A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Actomyosin Is the Main Driver of Interkinetic Nuclear Migration in the Retina</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>2009-09-18</date><risdate>2009</risdate><volume>138</volume><issue>6</issue><spage>1195</spage><epage>1208</epage><pages>1195-1208</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><abstract>Progenitor cell nuclei in the rapidly expanding epithelium of the embryonic vertebrate central nervous system undergo a process called interkinetic nuclear migration (IKNM). 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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Actomyosin - metabolism Animals Cell Nucleus - metabolism CELLBIO Danio rerio Dynactin Complex Embryo, Nonmammalian - cytology Embryo, Nonmammalian - metabolism Microtubule-Associated Proteins - metabolism MOLNEURO Neuroepithelial Cells - cytology Neuroepithelial Cells - metabolism Retina - cytology Retina - embryology Zebrafish - embryology Zebrafish - metabolism Zebrafish Proteins - metabolism |
| title | Actomyosin Is the Main Driver of Interkinetic Nuclear Migration in the Retina |
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