A simple taurocholate-induced model of severe acute pancreatitis in rats
AIM： To investigate gut barrier damage and intestinal bacteria translocation in severe acute pancreatitis （SAP）, a simple rat model of SAP was induced and studied. METHODS： Pancreatitis was induced by uniformly distributed injection of 3.8% Na taurocholate （1 mL/kg） beneath the pancreatic capsule. R...
Gespeichert in:
| Veröffentlicht in: | World journal of gastroenterology : WJG 2009-12, Vol.15 (45), p.5732-5739 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5739 |
|---|---|
| container_issue | 45 |
| container_start_page | 5732 |
| container_title | World journal of gastroenterology : WJG |
| container_volume | 15 |
| creator | Liu, Zhong-Hui Peng, Jun-Sheng Li, Chu-Jun Yang, Zu-Li Xiang, Jun Song, Hu Wu, Xiao-Bing Chen, Jun-Rong Diao, De-Chang |
| description | AIM： To investigate gut barrier damage and intestinal bacteria translocation in severe acute pancreatitis （SAP）, a simple rat model of SAP was induced and studied. METHODS： Pancreatitis was induced by uniformly distributed injection of 3.8% Na taurocholate （1 mL/kg） beneath the pancreatic capsule. Rats in the control group were injected with normal saline in the identical location. RESULTS： Serum amylase, plasma endotoxin, intestinal permeability, and pancreatitis pathology scores were all markedly higher in the pancreatitis group than in the control group （P 〈 0.01）. The bacterial infection rate was significantly higher in the SAP group than in the control group （P 〈 0.01）, observed in parallel by both bacterial culture and real-time polymerase chain reaction. Acute damage of the pancreas was observed histologically in SAP rats, showing interstitial edema, leukocyte infiltration, acinar cell necrosis and hemorrhage. The microstructure of the intestinal mucosa of SAP rats appeared to be destroyed with loose, shortened microvilli and rupture of the intercellular junction, as shown by electron microscopy. CONCLUSION： Significant gut barrier damage and intestinal bacterial translocation were definitely observed with few potential study confounders in this SAP rat model, suggesting that it may be an appropriate animal model for study of gut barrier damage and bacterial translocation in SAP. |
| doi_str_mv | 10.3748/wjg.15.5732 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2789229</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>32493890</cqvip_id><wanfj_id>wjg200945016</wanfj_id><sourcerecordid>wjg200945016</sourcerecordid><originalsourceid>FETCH-LOGICAL-c321t-b72778a81f87b6309a9fe9ecdde748b45ee1199bf297798e1c9cb894dbaa15a83</originalsourceid><addsrcrecordid>eNpVkM1Lw0AQxRdRbK2evMsiXlP3I-nuXgQpaoWCFz2HyWaSbk02MR8t_vcuVEVPc5g3v_fmEXLJ2VyqWN_ut-WcJ_NESXFEpkJwEwkds2My5YypyEihJuSs77eMCSkTcUom3JgFCwdTsrqnvavbCukAY9fYTVPBgJHz-Wgxp3WTY0Wbgva4ww4p2HFA2oK3HcLgBtdT52kHQ39OTgqoerz4njPy9vjwulxF65en5-X9OrJS8CHKlFBKg-aFVtlCMgOmQIM2zzH8ksUJIg_pskIYpYxGbo3NtInzDIAnoOWM3B247ZjVmFv0QwdV2nauhu4zbcCl_zfebdKy2aVCaSOECYCbA2APvgBfpttm7HyInIYiBWMmThhfBNnVX59fg5_qguD6IAid-fLDBVIG9r1wFaZSxEZqw-QXzQF84w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>A simple taurocholate-induced model of severe acute pancreatitis in rats</title><source>MEDLINE</source><source>Baishideng "World Journal of" online journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Liu, Zhong-Hui ; Peng, Jun-Sheng ; Li, Chu-Jun ; Yang, Zu-Li ; Xiang, Jun ; Song, Hu ; Wu, Xiao-Bing ; Chen, Jun-Rong ; Diao, De-Chang</creator><creatorcontrib>Liu, Zhong-Hui ; Peng, Jun-Sheng ; Li, Chu-Jun ; Yang, Zu-Li ; Xiang, Jun ; Song, Hu ; Wu, Xiao-Bing ; Chen, Jun-Rong ; Diao, De-Chang</creatorcontrib><description>AIM： To investigate gut barrier damage and intestinal bacteria translocation in severe acute pancreatitis （SAP）, a simple rat model of SAP was induced and studied. METHODS： Pancreatitis was induced by uniformly distributed injection of 3.8% Na taurocholate （1 mL/kg） beneath the pancreatic capsule. Rats in the control group were injected with normal saline in the identical location. RESULTS： Serum amylase, plasma endotoxin, intestinal permeability, and pancreatitis pathology scores were all markedly higher in the pancreatitis group than in the control group （P 〈 0.01）. The bacterial infection rate was significantly higher in the SAP group than in the control group （P 〈 0.01）, observed in parallel by both bacterial culture and real-time polymerase chain reaction. Acute damage of the pancreas was observed histologically in SAP rats, showing interstitial edema, leukocyte infiltration, acinar cell necrosis and hemorrhage. The microstructure of the intestinal mucosa of SAP rats appeared to be destroyed with loose, shortened microvilli and rupture of the intercellular junction, as shown by electron microscopy. CONCLUSION： Significant gut barrier damage and intestinal bacterial translocation were definitely observed with few potential study confounders in this SAP rat model, suggesting that it may be an appropriate animal model for study of gut barrier damage and bacterial translocation in SAP.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.15.5732</identifier><identifier>PMID: 19960573</identifier><language>eng</language><publisher>United States: The WJG Press and Baishideng</publisher><subject>Animals ; Brief ; Cholagogues and Choleretics - pharmacology ; Disease Models, Animal ; Female ; Male ; Pancreas - drug effects ; Pancreas - metabolism ; Pancreas - pathology ; Pancreatitis - chemically induced ; Pancreatitis - microbiology ; Pancreatitis - pathology ; Random Allocation ; Rats ; SAP ; Taurocholic Acid - pharmacology ; 模型势 ; 牛磺胆酸钠 ; 聚合酶链反应 ; 肠道细菌 ; 重症急性胰腺炎</subject><ispartof>World journal of gastroenterology : WJG, 2009-12, Vol.15 (45), p.5732-5739</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2009 The WJG Press and Baishideng. All rights reserved. 2009</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2789229/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2789229/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19960573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Zhong-Hui</creatorcontrib><creatorcontrib>Peng, Jun-Sheng</creatorcontrib><creatorcontrib>Li, Chu-Jun</creatorcontrib><creatorcontrib>Yang, Zu-Li</creatorcontrib><creatorcontrib>Xiang, Jun</creatorcontrib><creatorcontrib>Song, Hu</creatorcontrib><creatorcontrib>Wu, Xiao-Bing</creatorcontrib><creatorcontrib>Chen, Jun-Rong</creatorcontrib><creatorcontrib>Diao, De-Chang</creatorcontrib><title>A simple taurocholate-induced model of severe acute pancreatitis in rats</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM： To investigate gut barrier damage and intestinal bacteria translocation in severe acute pancreatitis （SAP）, a simple rat model of SAP was induced and studied. METHODS： Pancreatitis was induced by uniformly distributed injection of 3.8% Na taurocholate （1 mL/kg） beneath the pancreatic capsule. Rats in the control group were injected with normal saline in the identical location. RESULTS： Serum amylase, plasma endotoxin, intestinal permeability, and pancreatitis pathology scores were all markedly higher in the pancreatitis group than in the control group （P 〈 0.01）. The bacterial infection rate was significantly higher in the SAP group than in the control group （P 〈 0.01）, observed in parallel by both bacterial culture and real-time polymerase chain reaction. Acute damage of the pancreas was observed histologically in SAP rats, showing interstitial edema, leukocyte infiltration, acinar cell necrosis and hemorrhage. The microstructure of the intestinal mucosa of SAP rats appeared to be destroyed with loose, shortened microvilli and rupture of the intercellular junction, as shown by electron microscopy. CONCLUSION： Significant gut barrier damage and intestinal bacterial translocation were definitely observed with few potential study confounders in this SAP rat model, suggesting that it may be an appropriate animal model for study of gut barrier damage and bacterial translocation in SAP.</description><subject>Animals</subject><subject>Brief</subject><subject>Cholagogues and Choleretics - pharmacology</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Male</subject><subject>Pancreas - drug effects</subject><subject>Pancreas - metabolism</subject><subject>Pancreas - pathology</subject><subject>Pancreatitis - chemically induced</subject><subject>Pancreatitis - microbiology</subject><subject>Pancreatitis - pathology</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>SAP</subject><subject>Taurocholic Acid - pharmacology</subject><subject>模型势</subject><subject>牛磺胆酸钠</subject><subject>聚合酶链反应</subject><subject>肠道细菌</subject><subject>重症急性胰腺炎</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1Lw0AQxRdRbK2evMsiXlP3I-nuXgQpaoWCFz2HyWaSbk02MR8t_vcuVEVPc5g3v_fmEXLJ2VyqWN_ut-WcJ_NESXFEpkJwEwkds2My5YypyEihJuSs77eMCSkTcUom3JgFCwdTsrqnvavbCukAY9fYTVPBgJHz-Wgxp3WTY0Wbgva4ww4p2HFA2oK3HcLgBtdT52kHQ39OTgqoerz4njPy9vjwulxF65en5-X9OrJS8CHKlFBKg-aFVtlCMgOmQIM2zzH8ksUJIg_pskIYpYxGbo3NtInzDIAnoOWM3B247ZjVmFv0QwdV2nauhu4zbcCl_zfebdKy2aVCaSOECYCbA2APvgBfpttm7HyInIYiBWMmThhfBNnVX59fg5_qguD6IAid-fLDBVIG9r1wFaZSxEZqw-QXzQF84w</recordid><startdate>20091207</startdate><enddate>20091207</enddate><creator>Liu, Zhong-Hui</creator><creator>Peng, Jun-Sheng</creator><creator>Li, Chu-Jun</creator><creator>Yang, Zu-Li</creator><creator>Xiang, Jun</creator><creator>Song, Hu</creator><creator>Wu, Xiao-Bing</creator><creator>Chen, Jun-Rong</creator><creator>Diao, De-Chang</creator><general>The WJG Press and Baishideng</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W95</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20091207</creationdate><title>A simple taurocholate-induced model of severe acute pancreatitis in rats</title><author>Liu, Zhong-Hui ; Peng, Jun-Sheng ; Li, Chu-Jun ; Yang, Zu-Li ; Xiang, Jun ; Song, Hu ; Wu, Xiao-Bing ; Chen, Jun-Rong ; Diao, De-Chang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c321t-b72778a81f87b6309a9fe9ecdde748b45ee1199bf297798e1c9cb894dbaa15a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Brief</topic><topic>Cholagogues and Choleretics - pharmacology</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Male</topic><topic>Pancreas - drug effects</topic><topic>Pancreas - metabolism</topic><topic>Pancreas - pathology</topic><topic>Pancreatitis - chemically induced</topic><topic>Pancreatitis - microbiology</topic><topic>Pancreatitis - pathology</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>SAP</topic><topic>Taurocholic Acid - pharmacology</topic><topic>模型势</topic><topic>牛磺胆酸钠</topic><topic>聚合酶链反应</topic><topic>肠道细菌</topic><topic>重症急性胰腺炎</topic><toplevel>online_resources</toplevel><creatorcontrib>Liu, Zhong-Hui</creatorcontrib><creatorcontrib>Peng, Jun-Sheng</creatorcontrib><creatorcontrib>Li, Chu-Jun</creatorcontrib><creatorcontrib>Yang, Zu-Li</creatorcontrib><creatorcontrib>Xiang, Jun</creatorcontrib><creatorcontrib>Song, Hu</creatorcontrib><creatorcontrib>Wu, Xiao-Bing</creatorcontrib><creatorcontrib>Chen, Jun-Rong</creatorcontrib><creatorcontrib>Diao, De-Chang</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-农业科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Zhong-Hui</au><au>Peng, Jun-Sheng</au><au>Li, Chu-Jun</au><au>Yang, Zu-Li</au><au>Xiang, Jun</au><au>Song, Hu</au><au>Wu, Xiao-Bing</au><au>Chen, Jun-Rong</au><au>Diao, De-Chang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple taurocholate-induced model of severe acute pancreatitis in rats</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2009-12-07</date><risdate>2009</risdate><volume>15</volume><issue>45</issue><spage>5732</spage><epage>5739</epage><pages>5732-5739</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM： To investigate gut barrier damage and intestinal bacteria translocation in severe acute pancreatitis （SAP）, a simple rat model of SAP was induced and studied. METHODS： Pancreatitis was induced by uniformly distributed injection of 3.8% Na taurocholate （1 mL/kg） beneath the pancreatic capsule. Rats in the control group were injected with normal saline in the identical location. RESULTS： Serum amylase, plasma endotoxin, intestinal permeability, and pancreatitis pathology scores were all markedly higher in the pancreatitis group than in the control group （P 〈 0.01）. The bacterial infection rate was significantly higher in the SAP group than in the control group （P 〈 0.01）, observed in parallel by both bacterial culture and real-time polymerase chain reaction. Acute damage of the pancreas was observed histologically in SAP rats, showing interstitial edema, leukocyte infiltration, acinar cell necrosis and hemorrhage. The microstructure of the intestinal mucosa of SAP rats appeared to be destroyed with loose, shortened microvilli and rupture of the intercellular junction, as shown by electron microscopy. CONCLUSION： Significant gut barrier damage and intestinal bacterial translocation were definitely observed with few potential study confounders in this SAP rat model, suggesting that it may be an appropriate animal model for study of gut barrier damage and bacterial translocation in SAP.</abstract><cop>United States</cop><pub>The WJG Press and Baishideng</pub><pmid>19960573</pmid><doi>10.3748/wjg.15.5732</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1007-9327 |
| ispartof | World journal of gastroenterology : WJG, 2009-12, Vol.15 (45), p.5732-5739 |
| issn | 1007-9327 2219-2840 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2789229 |
| source | MEDLINE; Baishideng "World Journal of" online journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Brief Cholagogues and Choleretics - pharmacology Disease Models, Animal Female Male Pancreas - drug effects Pancreas - metabolism Pancreas - pathology Pancreatitis - chemically induced Pancreatitis - microbiology Pancreatitis - pathology Random Allocation Rats SAP Taurocholic Acid - pharmacology 模型势 牛磺胆酸钠 聚合酶链反应 肠道细菌 重症急性胰腺炎 |
| title | A simple taurocholate-induced model of severe acute pancreatitis in rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T00%3A26%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20simple%20taurocholate-induced%20model%20of%20severe%20acute%20pancreatitis%20in%20rats&rft.jtitle=World%20journal%20of%20gastroenterology%20:%20WJG&rft.au=Liu,%20Zhong-Hui&rft.date=2009-12-07&rft.volume=15&rft.issue=45&rft.spage=5732&rft.epage=5739&rft.pages=5732-5739&rft.issn=1007-9327&rft.eissn=2219-2840&rft_id=info:doi/10.3748/wjg.15.5732&rft_dat=%3Cwanfang_jour_pubme%3Ewjg200945016%3C/wanfang_jour_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/19960573&rft_cqvip_id=32493890&rft_wanfj_id=wjg200945016&rfr_iscdi=true |