Combining Single-Molecule Optical Trapping and Small-Angle X-Ray Scattering Measurements to Compute the Persistence Length of a Protein ER/K α-Helix

A relatively unknown protein structure motif forms stable isolated single α-helices, termed ER/K α-helices, in a wide variety of proteins and has been shown to be essential for the function of some molecular motors. The flexibility of the ER/K α-helix determines whether it behaves as a force transdu...

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Veröffentlicht in:Biophysical journal 2009-12, Vol.97 (11), p.2993-2999
Hauptverfasser: Sivaramakrishnan, S., Sung, J., Ali, M., Doniach, S., Flyvbjerg, H., Spudich, J.A.
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Sprache:eng
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