Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas
AIM： To investigate the expression and function of Wolfram syndrome 1 gene （WFS1） during the development of normal pancreas. METHODS： Pancreas from Sprague-Dawley rat fetuses, embryos, young and adult animals were used in this study. Expression levels of WFS1 in pancreas of different development sta...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2009-11, Vol.15 (43), p.5425-5431 |
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| creator | Xu, Rong Xia, Biao Geng, Jie Shi, Jing Shi, Hui Yuan, Li De, Wei |
| description | AIM： To investigate the expression and function of Wolfram syndrome 1 gene （WFS1） during the development of normal pancreas. METHODS： Pancreas from Sprague-Dawley rat fetuses, embryos, young and adult animals were used in this study. Expression levels of WFS1 in pancreas of different development stages were detected by reverse transcription-polymerase chain reation （RT-PCR） and Western blotting. To identify the cell location of WFS1 in the developing rat pancreas, double-immunofluorescent staining was performed using antibodies to specific cell markers and WFS1, respectively. RESULTS： Compared to E15.5, the highest level of WFSl mRNA was detected at E18.5, the level of WFSl mRNA in E15.5 and P0 was less, and at a lowest at adult （P 〈 0.05 vs P0 and adult）, respectively. Compare to E15.5, the highest level of WFS1 was at P14 and lowest at P21 （P 〈 0.05 vs P14 and P21）, respectively. The WFSl positive staining is expressed in the normal developing rat pancreas mainly in the islet beta-cells and mesenchyme at each stage tested. CONCLUSION： These results indicate that WFSl may be involved in some aspects of pancreatic development and further research on WFS1 may provide new evidences to prove the interactions between mesenchyma and epithelia at the same time. |
| doi_str_mv | 10.3748/wjg.15.5425 |
| format | Article |
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METHODS： Pancreas from Sprague-Dawley rat fetuses, embryos, young and adult animals were used in this study. Expression levels of WFS1 in pancreas of different development stages were detected by reverse transcription-polymerase chain reation （RT-PCR） and Western blotting. To identify the cell location of WFS1 in the developing rat pancreas, double-immunofluorescent staining was performed using antibodies to specific cell markers and WFS1, respectively. RESULTS： Compared to E15.5, the highest level of WFSl mRNA was detected at E18.5, the level of WFSl mRNA in E15.5 and P0 was less, and at a lowest at adult （P 〈 0.05 vs P0 and adult）, respectively. Compare to E15.5, the highest level of WFS1 was at P14 and lowest at P21 （P 〈 0.05 vs P14 and P21）, respectively. The WFSl positive staining is expressed in the normal developing rat pancreas mainly in the islet beta-cells and mesenchyme at each stage tested. CONCLUSION： These results indicate that WFSl may be involved in some aspects of pancreatic development and further research on WFS1 may provide new evidences to prove the interactions between mesenchyma and epithelia at the same time.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.15.5425</identifier><identifier>PMID: 19916172</identifier><language>eng</language><publisher>United States: Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China%First School of Clinical Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China</publisher><subject>Animals ; Exons ; Extracellular Matrix - metabolism ; Gene Expression Regulation, Developmental ; Immunohistochemistry - methods ; Insulin-Secreting Cells - cytology ; Membrane Proteins - biosynthesis ; Membrane Proteins - genetics ; Mesoderm - metabolism ; Original ; Pancreas - embryology ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; SD大鼠 ; Time Factors ; 免疫印迹 ; 发展中国家 ; 基因定位 ; 细胞标记 ; 综合征 ; 胰腺癌 ; 逆转录聚合酶链反应</subject><ispartof>World journal of gastroenterology : WJG, 2009-11, Vol.15 (43), p.5425-5431</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2009 The WJG Press and Baishideng. All rights reserved. 2009</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-e1ea9a21029de30eb28c11475aa2fafaa80e8b35e1a2ae002b64e9b1cedad8ac3</citedby><cites>FETCH-LOGICAL-c399t-e1ea9a21029de30eb28c11475aa2fafaa80e8b35e1a2ae002b64e9b1cedad8ac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2778098/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2778098/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19916172$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Rong</creatorcontrib><creatorcontrib>Xia, Biao</creatorcontrib><creatorcontrib>Geng, Jie</creatorcontrib><creatorcontrib>Shi, Jing</creatorcontrib><creatorcontrib>Shi, Hui</creatorcontrib><creatorcontrib>Yuan, Li</creatorcontrib><creatorcontrib>De, Wei</creatorcontrib><title>Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM： To investigate the expression and function of Wolfram syndrome 1 gene （WFS1） during the development of normal pancreas. METHODS： Pancreas from Sprague-Dawley rat fetuses, embryos, young and adult animals were used in this study. Expression levels of WFS1 in pancreas of different development stages were detected by reverse transcription-polymerase chain reation （RT-PCR） and Western blotting. To identify the cell location of WFS1 in the developing rat pancreas, double-immunofluorescent staining was performed using antibodies to specific cell markers and WFS1, respectively. RESULTS： Compared to E15.5, the highest level of WFSl mRNA was detected at E18.5, the level of WFSl mRNA in E15.5 and P0 was less, and at a lowest at adult （P 〈 0.05 vs P0 and adult）, respectively. Compare to E15.5, the highest level of WFS1 was at P14 and lowest at P21 （P 〈 0.05 vs P14 and P21）, respectively. The WFSl positive staining is expressed in the normal developing rat pancreas mainly in the islet beta-cells and mesenchyme at each stage tested. CONCLUSION： These results indicate that WFSl may be involved in some aspects of pancreatic development and further research on WFS1 may provide new evidences to prove the interactions between mesenchyma and epithelia at the same time.</description><subject>Animals</subject><subject>Exons</subject><subject>Extracellular Matrix - metabolism</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Immunohistochemistry - methods</subject><subject>Insulin-Secreting Cells - cytology</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - genetics</subject><subject>Mesoderm - metabolism</subject><subject>Original</subject><subject>Pancreas - embryology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>SD大鼠</subject><subject>Time Factors</subject><subject>免疫印迹</subject><subject>发展中国家</subject><subject>基因定位</subject><subject>细胞标记</subject><subject>综合征</subject><subject>胰腺癌</subject><subject>逆转录聚合酶链反应</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUtr3DAURkVpaSZpV90XUQpZFE_1cmRtAiWkDwh009CluJavPZ7KkiN5kqa_vhpm6GMluDqc-3E_Ql5xtpZaNe8ftsOa1-taifoJWQnBTSUaxZ6SFWdMV0YKfUJOc94yJqSsxXNywo3hF1yLFbm9_jknzHmMgULoqI8O_PgLlv0g9vR79H2CiebH0KU4IeV0wIB0DHTZIO3wHn2cxzDQBAudIbiEkF-QZz34jC-P7xm5_Xj97epzdfP105erDzeVk8YsFXIEA4IzYTqUDFvROM6VrgFEDz1Aw7BpZY0cBGCJ314oNC132EHXgJNn5PLgnXfthJ3DsCTwdk7jBOnRRhjt_z9h3Ngh3luhdcNMUwRvD4IHCD2EwW7jLoUS2ZarCsaMkozVBTs_7knxbod5sdOYHXoPAeMuWy0VV6LRqpDvDqRLMeeE_Z8wnNl9XXux5bXd11Xo1__m_8se-ynAm6NuE8NwV-5sW3A_-tGjlUIZaUqnvwGwbp4m</recordid><startdate>20091121</startdate><enddate>20091121</enddate><creator>Xu, Rong</creator><creator>Xia, Biao</creator><creator>Geng, Jie</creator><creator>Shi, Jing</creator><creator>Shi, Hui</creator><creator>Yuan, Li</creator><creator>De, Wei</creator><general>Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China%First School of Clinical Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China</general><general>The WJG Press and Baishideng</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20091121</creationdate><title>Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas</title><author>Xu, Rong ; Xia, Biao ; Geng, Jie ; Shi, Jing ; Shi, Hui ; Yuan, Li ; De, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-e1ea9a21029de30eb28c11475aa2fafaa80e8b35e1a2ae002b64e9b1cedad8ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Exons</topic><topic>Extracellular Matrix - metabolism</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Immunohistochemistry - methods</topic><topic>Insulin-Secreting Cells - cytology</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - genetics</topic><topic>Mesoderm - metabolism</topic><topic>Original</topic><topic>Pancreas - embryology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>SD大鼠</topic><topic>Time Factors</topic><topic>免疫印迹</topic><topic>发展中国家</topic><topic>基因定位</topic><topic>细胞标记</topic><topic>综合征</topic><topic>胰腺癌</topic><topic>逆转录聚合酶链反应</topic><toplevel>online_resources</toplevel><creatorcontrib>Xu, Rong</creatorcontrib><creatorcontrib>Xia, Biao</creatorcontrib><creatorcontrib>Geng, Jie</creatorcontrib><creatorcontrib>Shi, Jing</creatorcontrib><creatorcontrib>Shi, Hui</creatorcontrib><creatorcontrib>Yuan, Li</creatorcontrib><creatorcontrib>De, Wei</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Rong</au><au>Xia, Biao</au><au>Geng, Jie</au><au>Shi, Jing</au><au>Shi, Hui</au><au>Yuan, Li</au><au>De, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2009-11-21</date><risdate>2009</risdate><volume>15</volume><issue>43</issue><spage>5425</spage><epage>5431</epage><pages>5425-5431</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM： To investigate the expression and function of Wolfram syndrome 1 gene （WFS1） during the development of normal pancreas. METHODS： Pancreas from Sprague-Dawley rat fetuses, embryos, young and adult animals were used in this study. Expression levels of WFS1 in pancreas of different development stages were detected by reverse transcription-polymerase chain reation （RT-PCR） and Western blotting. To identify the cell location of WFS1 in the developing rat pancreas, double-immunofluorescent staining was performed using antibodies to specific cell markers and WFS1, respectively. RESULTS： Compared to E15.5, the highest level of WFSl mRNA was detected at E18.5, the level of WFSl mRNA in E15.5 and P0 was less, and at a lowest at adult （P 〈 0.05 vs P0 and adult）, respectively. Compare to E15.5, the highest level of WFS1 was at P14 and lowest at P21 （P 〈 0.05 vs P14 and P21）, respectively. The WFSl positive staining is expressed in the normal developing rat pancreas mainly in the islet beta-cells and mesenchyme at each stage tested. CONCLUSION： These results indicate that WFSl may be involved in some aspects of pancreatic development and further research on WFS1 may provide new evidences to prove the interactions between mesenchyma and epithelia at the same time.</abstract><cop>United States</cop><pub>Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China%First School of Clinical Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China</pub><pmid>19916172</pmid><doi>10.3748/wjg.15.5425</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Baishideng "World Journal of" online journals; PubMed Central; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals Exons Extracellular Matrix - metabolism Gene Expression Regulation, Developmental Immunohistochemistry - methods Insulin-Secreting Cells - cytology Membrane Proteins - biosynthesis Membrane Proteins - genetics Mesoderm - metabolism Original Pancreas - embryology Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism SD大鼠 Time Factors 免疫印迹 发展中国家 基因定位 细胞标记 综合征 胰腺癌 逆转录聚合酶链反应 |
| title | Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas |
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