The angiogenic effect of probiotic Bacillus polyfermenticus on human intestinal microvascular endothelial cells is mediated by IL-8
Angiogenesis is required for wound healing and repair, but dysregulated angiogenesis is involved in gastrointestinal inflammation. Bacillus polyfermenticus (B.P.) is a probiotic bacterium clinically used for a variety of intestinal disorders in East Asia. Here we investigated the effect of B.P. on a...
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| Veröffentlicht in: | American journal of physiology: Gastrointestinal and liver physiology 2009-11, Vol.297 (5), p.G999-G1008 |
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| container_title | American journal of physiology: Gastrointestinal and liver physiology |
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| creator | Im, Eunok Choi, Yoon Jeong Kim, Cho Hee Fiocchi, Claudio Pothoulakis, Charalabos Rhee, Sang Hoon |
| description | Angiogenesis is required for wound healing and repair, but dysregulated angiogenesis is involved in gastrointestinal inflammation. Bacillus polyfermenticus (B.P.) is a probiotic bacterium clinically used for a variety of intestinal disorders in East Asia. Here we investigated the effect of B.P. on angiogenesis of human intestinal microvascular endothelial cells (HIMECs) and wound healing in intestinal mucosa. Exposure of HIMECs to the conditioned medium of B.P. cultures (B.P. CM) increased cell migration, permeability, and tube formation. Production of the proangiogenic cytokine IL-8 was increased by B.P. CM, and neutralizing antibodies against IL-8 or IL-8 receptor CXCR2 reduced tube formation as well as actin stress fiber formation. B.P. CM also increased NF-kappaB activation, and inhibitors of NF-kappaB suppressed B.P. CM-induced tube formation and IL-8 production. Furthermore, B.P. facilitated recovery of mice from colitis as shown by increased body weight and reduced rectal bleeding and histological severity. B.P. also increased angiogenesis and mouse IL-8 production in the mucosal layer. Collectively, these results show that B.P. increases angiogenesis of HIMECs in a NF-kappaB/IL-8/CXCR2-dependent manner. Moreover, B.P. promotes angiogenesis in the mucosa during recovery of mice from colitis, suggesting that this probiotic may be clinically used to facilitate intestinal wound healing. |
| doi_str_mv | 10.1152/ajpgi.00204.2009 |
| format | Article |
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Bacillus polyfermenticus (B.P.) is a probiotic bacterium clinically used for a variety of intestinal disorders in East Asia. Here we investigated the effect of B.P. on angiogenesis of human intestinal microvascular endothelial cells (HIMECs) and wound healing in intestinal mucosa. Exposure of HIMECs to the conditioned medium of B.P. cultures (B.P. CM) increased cell migration, permeability, and tube formation. Production of the proangiogenic cytokine IL-8 was increased by B.P. CM, and neutralizing antibodies against IL-8 or IL-8 receptor CXCR2 reduced tube formation as well as actin stress fiber formation. B.P. CM also increased NF-kappaB activation, and inhibitors of NF-kappaB suppressed B.P. CM-induced tube formation and IL-8 production. Furthermore, B.P. facilitated recovery of mice from colitis as shown by increased body weight and reduced rectal bleeding and histological severity. B.P. also increased angiogenesis and mouse IL-8 production in the mucosal layer. Collectively, these results show that B.P. increases angiogenesis of HIMECs in a NF-kappaB/IL-8/CXCR2-dependent manner. Moreover, B.P. promotes angiogenesis in the mucosa during recovery of mice from colitis, suggesting that this probiotic may be clinically used to facilitate intestinal wound healing.</description><identifier>ISSN: 0193-1857</identifier><identifier>EISSN: 1522-1547</identifier><identifier>DOI: 10.1152/ajpgi.00204.2009</identifier><identifier>PMID: 20501448</identifier><identifier>CODEN: APGPDF</identifier><language>eng</language><publisher>United States: American Physiological Society</publisher><subject>Animals ; Bacillus - metabolism ; Capillary Permeability - drug effects ; Cell adhesion & migration ; Cell culture ; Cell Movement - drug effects ; Cell Proliferation - drug effects ; Cells, Cultured ; Colitis, Ulcerative - chemically induced ; Colitis, Ulcerative - pathology ; Colitis, Ulcerative - therapy ; Culture Media, Conditioned - pharmacology ; Cytokines ; Endothelial Cells - cytology ; Endothelial Cells - drug effects ; Endothelial Cells - metabolism ; Endothelial Cells - physiology ; Gene Expression - drug effects ; Gene Expression - genetics ; Humans ; Inflammation/Immunity/Mediators ; Inflammatory bowel disease ; Interleukin-8 - metabolism ; Interleukin-8 - pharmacology ; Intestines - blood supply ; Intestines - microbiology ; Male ; Mice ; Mice, Inbred Strains ; Neovascularization, Physiologic - drug effects ; Neovascularization, Physiologic - physiology ; NF-kappa B - antagonists & inhibitors ; NF-kappa B - metabolism ; Phenylurea Compounds - pharmacology ; Phosphorylation - drug effects ; Physiology ; Probiotics ; Probiotics - metabolism ; Probiotics - therapeutic use ; Receptors, Interleukin-8B - antagonists & inhibitors ; Receptors, Interleukin-8B - genetics ; Receptors, Interleukin-8B - metabolism ; Rodents ; Stress Fibers - drug effects ; Stress Fibers - metabolism ; Vascular Endothelial Growth Factor A - pharmacology ; Wound Healing - drug effects ; Wound Healing - physiology</subject><ispartof>American journal of physiology: Gastrointestinal and liver physiology, 2009-11, Vol.297 (5), p.G999-G1008</ispartof><rights>Copyright American Physiological Society Nov 2009</rights><rights>Copyright © 2009 the American Physiological Society 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-85d83222f660e74251a79194a4f631313a8533dc1b9dac1cb2e01daba1359fe13</citedby><cites>FETCH-LOGICAL-c422t-85d83222f660e74251a79194a4f631313a8533dc1b9dac1cb2e01daba1359fe13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20501448$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Im, Eunok</creatorcontrib><creatorcontrib>Choi, Yoon Jeong</creatorcontrib><creatorcontrib>Kim, Cho Hee</creatorcontrib><creatorcontrib>Fiocchi, Claudio</creatorcontrib><creatorcontrib>Pothoulakis, Charalabos</creatorcontrib><creatorcontrib>Rhee, Sang Hoon</creatorcontrib><title>The angiogenic effect of probiotic Bacillus polyfermenticus on human intestinal microvascular endothelial cells is mediated by IL-8</title><title>American journal of physiology: Gastrointestinal and liver physiology</title><addtitle>Am J Physiol Gastrointest Liver Physiol</addtitle><description>Angiogenesis is required for wound healing and repair, but dysregulated angiogenesis is involved in gastrointestinal inflammation. Bacillus polyfermenticus (B.P.) is a probiotic bacterium clinically used for a variety of intestinal disorders in East Asia. Here we investigated the effect of B.P. on angiogenesis of human intestinal microvascular endothelial cells (HIMECs) and wound healing in intestinal mucosa. Exposure of HIMECs to the conditioned medium of B.P. cultures (B.P. CM) increased cell migration, permeability, and tube formation. Production of the proangiogenic cytokine IL-8 was increased by B.P. CM, and neutralizing antibodies against IL-8 or IL-8 receptor CXCR2 reduced tube formation as well as actin stress fiber formation. B.P. CM also increased NF-kappaB activation, and inhibitors of NF-kappaB suppressed B.P. CM-induced tube formation and IL-8 production. Furthermore, B.P. facilitated recovery of mice from colitis as shown by increased body weight and reduced rectal bleeding and histological severity. B.P. also increased angiogenesis and mouse IL-8 production in the mucosal layer. Collectively, these results show that B.P. increases angiogenesis of HIMECs in a NF-kappaB/IL-8/CXCR2-dependent manner. Moreover, B.P. promotes angiogenesis in the mucosa during recovery of mice from colitis, suggesting that this probiotic may be clinically used to facilitate intestinal wound healing.</description><subject>Animals</subject><subject>Bacillus - metabolism</subject><subject>Capillary Permeability - drug effects</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cell Movement - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Colitis, Ulcerative - chemically induced</subject><subject>Colitis, Ulcerative - pathology</subject><subject>Colitis, Ulcerative - therapy</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Cytokines</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelial Cells - metabolism</subject><subject>Endothelial Cells - physiology</subject><subject>Gene Expression - drug effects</subject><subject>Gene Expression - genetics</subject><subject>Humans</subject><subject>Inflammation/Immunity/Mediators</subject><subject>Inflammatory bowel disease</subject><subject>Interleukin-8 - metabolism</subject><subject>Interleukin-8 - pharmacology</subject><subject>Intestines - blood supply</subject><subject>Intestines - microbiology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>NF-kappa B - antagonists & inhibitors</subject><subject>NF-kappa B - metabolism</subject><subject>Phenylurea Compounds - pharmacology</subject><subject>Phosphorylation - drug effects</subject><subject>Physiology</subject><subject>Probiotics</subject><subject>Probiotics - metabolism</subject><subject>Probiotics - therapeutic use</subject><subject>Receptors, Interleukin-8B - antagonists & inhibitors</subject><subject>Receptors, Interleukin-8B - genetics</subject><subject>Receptors, Interleukin-8B - metabolism</subject><subject>Rodents</subject><subject>Stress Fibers - drug effects</subject><subject>Stress Fibers - metabolism</subject><subject>Vascular Endothelial Growth Factor A - pharmacology</subject><subject>Wound Healing - drug effects</subject><subject>Wound Healing - physiology</subject><issn>0193-1857</issn><issn>1522-1547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1v3CAQRVGrZLPtvacK9e4tA2ZtXyq1UZJGWqmX9IzGGHZZYXDBjrTn_vGSj0atOCDeMG_mvUfIB2AbAMk_43Hauw1jnNUbzlh3RlYF5hXIunlDVgw6UUErmwtymfORMSY5wDm54EwyqOt2RX7fHwzFsHdxb4LT1Fhr9EyjpVOKvYtzwb6hdt4vmU7Rn6xJowkFLu8Y6GEZMVAXZpNnF9DT0ekUHzDrxWOiJgxxPhjvSkUb7zN1mY5mcDibgfYnerer2nfkrUWfzfuXe01-3lzfX32vdj9u766-7ipdcz5XrRxawTm32y0zTc0lYNNBV2NttwLKwVYKMWjouwE16J4bBgP2CEJ21oBYky_PvNPSlx10kZHQqym5EdNJRXTq_0pwB7WPD4o3TVNvWSH49EKQ4q-lKFbHuKSiOisuuCxGF8PXhD1_Kj7knIx9HQBMPaamnlJTT6mpx9RKy8d_F3tt-BuT-ANuhJbi</recordid><startdate>20091101</startdate><enddate>20091101</enddate><creator>Im, Eunok</creator><creator>Choi, Yoon Jeong</creator><creator>Kim, Cho Hee</creator><creator>Fiocchi, Claudio</creator><creator>Pothoulakis, Charalabos</creator><creator>Rhee, Sang Hoon</creator><general>American Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20091101</creationdate><title>The angiogenic effect of probiotic Bacillus polyfermenticus on human intestinal microvascular endothelial cells is mediated by IL-8</title><author>Im, Eunok ; Choi, Yoon Jeong ; Kim, Cho Hee ; Fiocchi, Claudio ; Pothoulakis, Charalabos ; Rhee, Sang Hoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-85d83222f660e74251a79194a4f631313a8533dc1b9dac1cb2e01daba1359fe13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Bacillus - metabolism</topic><topic>Capillary Permeability - drug effects</topic><topic>Cell adhesion & migration</topic><topic>Cell culture</topic><topic>Cell Movement - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Colitis, Ulcerative - chemically induced</topic><topic>Colitis, Ulcerative - pathology</topic><topic>Colitis, Ulcerative - therapy</topic><topic>Culture Media, Conditioned - pharmacology</topic><topic>Cytokines</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelial Cells - metabolism</topic><topic>Endothelial Cells - physiology</topic><topic>Gene Expression - drug effects</topic><topic>Gene Expression - genetics</topic><topic>Humans</topic><topic>Inflammation/Immunity/Mediators</topic><topic>Inflammatory bowel disease</topic><topic>Interleukin-8 - metabolism</topic><topic>Interleukin-8 - pharmacology</topic><topic>Intestines - blood supply</topic><topic>Intestines - microbiology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>NF-kappa B - antagonists & inhibitors</topic><topic>NF-kappa B - metabolism</topic><topic>Phenylurea Compounds - pharmacology</topic><topic>Phosphorylation - drug effects</topic><topic>Physiology</topic><topic>Probiotics</topic><topic>Probiotics - metabolism</topic><topic>Probiotics - therapeutic use</topic><topic>Receptors, Interleukin-8B - antagonists & inhibitors</topic><topic>Receptors, Interleukin-8B - genetics</topic><topic>Receptors, Interleukin-8B - metabolism</topic><topic>Rodents</topic><topic>Stress Fibers - drug effects</topic><topic>Stress Fibers - metabolism</topic><topic>Vascular Endothelial Growth Factor A - pharmacology</topic><topic>Wound Healing - drug effects</topic><topic>Wound Healing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Im, Eunok</creatorcontrib><creatorcontrib>Choi, Yoon Jeong</creatorcontrib><creatorcontrib>Kim, Cho Hee</creatorcontrib><creatorcontrib>Fiocchi, Claudio</creatorcontrib><creatorcontrib>Pothoulakis, Charalabos</creatorcontrib><creatorcontrib>Rhee, Sang Hoon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>American journal of physiology: Gastrointestinal and liver physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Im, Eunok</au><au>Choi, Yoon Jeong</au><au>Kim, Cho Hee</au><au>Fiocchi, Claudio</au><au>Pothoulakis, Charalabos</au><au>Rhee, Sang Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The angiogenic effect of probiotic Bacillus polyfermenticus on human intestinal microvascular endothelial cells is mediated by IL-8</atitle><jtitle>American journal of physiology: Gastrointestinal and liver physiology</jtitle><addtitle>Am J Physiol Gastrointest Liver Physiol</addtitle><date>2009-11-01</date><risdate>2009</risdate><volume>297</volume><issue>5</issue><spage>G999</spage><epage>G1008</epage><pages>G999-G1008</pages><issn>0193-1857</issn><eissn>1522-1547</eissn><coden>APGPDF</coden><abstract>Angiogenesis is required for wound healing and repair, but dysregulated angiogenesis is involved in gastrointestinal inflammation. Bacillus polyfermenticus (B.P.) is a probiotic bacterium clinically used for a variety of intestinal disorders in East Asia. Here we investigated the effect of B.P. on angiogenesis of human intestinal microvascular endothelial cells (HIMECs) and wound healing in intestinal mucosa. Exposure of HIMECs to the conditioned medium of B.P. cultures (B.P. CM) increased cell migration, permeability, and tube formation. Production of the proangiogenic cytokine IL-8 was increased by B.P. CM, and neutralizing antibodies against IL-8 or IL-8 receptor CXCR2 reduced tube formation as well as actin stress fiber formation. B.P. CM also increased NF-kappaB activation, and inhibitors of NF-kappaB suppressed B.P. CM-induced tube formation and IL-8 production. Furthermore, B.P. facilitated recovery of mice from colitis as shown by increased body weight and reduced rectal bleeding and histological severity. B.P. also increased angiogenesis and mouse IL-8 production in the mucosal layer. Collectively, these results show that B.P. increases angiogenesis of HIMECs in a NF-kappaB/IL-8/CXCR2-dependent manner. Moreover, B.P. promotes angiogenesis in the mucosa during recovery of mice from colitis, suggesting that this probiotic may be clinically used to facilitate intestinal wound healing.</abstract><cop>United States</cop><pub>American Physiological Society</pub><pmid>20501448</pmid><doi>10.1152/ajpgi.00204.2009</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0193-1857 |
| ispartof | American journal of physiology: Gastrointestinal and liver physiology, 2009-11, Vol.297 (5), p.G999-G1008 |
| issn | 0193-1857 1522-1547 |
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| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Bacillus - metabolism Capillary Permeability - drug effects Cell adhesion & migration Cell culture Cell Movement - drug effects Cell Proliferation - drug effects Cells, Cultured Colitis, Ulcerative - chemically induced Colitis, Ulcerative - pathology Colitis, Ulcerative - therapy Culture Media, Conditioned - pharmacology Cytokines Endothelial Cells - cytology Endothelial Cells - drug effects Endothelial Cells - metabolism Endothelial Cells - physiology Gene Expression - drug effects Gene Expression - genetics Humans Inflammation/Immunity/Mediators Inflammatory bowel disease Interleukin-8 - metabolism Interleukin-8 - pharmacology Intestines - blood supply Intestines - microbiology Male Mice Mice, Inbred Strains Neovascularization, Physiologic - drug effects Neovascularization, Physiologic - physiology NF-kappa B - antagonists & inhibitors NF-kappa B - metabolism Phenylurea Compounds - pharmacology Phosphorylation - drug effects Physiology Probiotics Probiotics - metabolism Probiotics - therapeutic use Receptors, Interleukin-8B - antagonists & inhibitors Receptors, Interleukin-8B - genetics Receptors, Interleukin-8B - metabolism Rodents Stress Fibers - drug effects Stress Fibers - metabolism Vascular Endothelial Growth Factor A - pharmacology Wound Healing - drug effects Wound Healing - physiology |
| title | The angiogenic effect of probiotic Bacillus polyfermenticus on human intestinal microvascular endothelial cells is mediated by IL-8 |
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