Noninvasive estimation of cell cycle phase and proliferation rate of human mesenchymal stem cells by phase-shifting laser microscopy
The simultaneous determination of the cell cycle phase of individual adherent mesenchymal stem cells (MSCs) using a fluorescence microscope after staining with 4′,6-diamidine-2′-phenylindole dihydrochloride and bromodeoxyuridine and the laser phase shift by phase-shifting laser microscopy (PLM) reve...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2009-04, Vol.59 (3), p.161-167 |
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| creator | Tokumitsu, Ayako Wakitani, Shigeyuki Takagi, Mutsumi |
| description | The simultaneous determination of the cell cycle phase of individual adherent mesenchymal stem cells (MSCs) using a fluorescence microscope after staining with 4′,6-diamidine-2′-phenylindole dihydrochloride and bromodeoxyuridine and the laser phase shift by phase-shifting laser microscopy (PLM) revealed that the laser phase shift of cells in the G
2
/M phase was markedly higher than that of cells in the G
0
/G
1
phase. Even in the synchronous cultures to G
0
/G
1
and G
2
/M cell cycle phases, the laser phase shift of the cells in the G
2
/M phase was markedly higher than that of the cells in the G
0
/G
1
phase. The analysis of the cultures of MSCs from different donors with the addition of FGF2 at different concentrations revealed that there was a marked negative correlation between the average phase shift and mean generation time. In conclusion, it is possible to estimate noninvasively the proliferation activity of MSCs population by measuring the phase shift using PLM. |
| doi_str_mv | 10.1007/s10616-009-9209-9 |
| format | Article |
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2
/M phase was markedly higher than that of cells in the G
0
/G
1
phase. Even in the synchronous cultures to G
0
/G
1
and G
2
/M cell cycle phases, the laser phase shift of the cells in the G
2
/M phase was markedly higher than that of the cells in the G
0
/G
1
phase. The analysis of the cultures of MSCs from different donors with the addition of FGF2 at different concentrations revealed that there was a marked negative correlation between the average phase shift and mean generation time. In conclusion, it is possible to estimate noninvasively the proliferation activity of MSCs population by measuring the phase shift using PLM.</description><identifier>ISSN: 0920-9069</identifier><identifier>EISSN: 1573-0778</identifier><identifier>DOI: 10.1007/s10616-009-9209-9</identifier><identifier>PMID: 19693683</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antibodies ; Biochemistry ; Biological and medical sciences ; Biomedicine ; Biotechnology ; Bone marrow ; Bromodeoxyuridine ; Cell culture ; Cell cycle ; Cell proliferation ; Chemistry ; Chemistry and Materials Science ; Fibroblast growth factor 2 ; Fibroblasts ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; G1 phase ; JAACT Special Issue ; Lasers ; Mesenchymal stem cells ; Mesenchyme ; Microscopes ; Microscopy ; Morphology ; Penicillin ; Phase shift ; Process controls ; Statistical analysis ; Stem cells ; Synchronous culture</subject><ispartof>Cytotechnology (Dordrecht), 2009-04, Vol.59 (3), p.161-167</ispartof><rights>Springer Science+Business Media B.V. 2009</rights><rights>2015 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2009.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c598t-f4da5410f7d4f9fd7e29cb6f69b2749803357a17f11359e6adadc1be26f752853</citedby><cites>FETCH-LOGICAL-c598t-f4da5410f7d4f9fd7e29cb6f69b2749803357a17f11359e6adadc1be26f752853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774568/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918257813?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,309,310,314,724,777,781,786,787,882,21369,23911,23912,25121,27905,27906,33725,33726,41469,42538,43786,51300,53772,53774,64364,64366,64368,72218</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22250055$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19693683$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tokumitsu, Ayako</creatorcontrib><creatorcontrib>Wakitani, Shigeyuki</creatorcontrib><creatorcontrib>Takagi, Mutsumi</creatorcontrib><title>Noninvasive estimation of cell cycle phase and proliferation rate of human mesenchymal stem cells by phase-shifting laser microscopy</title><title>Cytotechnology (Dordrecht)</title><addtitle>Cytotechnology</addtitle><addtitle>Cytotechnology</addtitle><description>The simultaneous determination of the cell cycle phase of individual adherent mesenchymal stem cells (MSCs) using a fluorescence microscope after staining with 4′,6-diamidine-2′-phenylindole dihydrochloride and bromodeoxyuridine and the laser phase shift by phase-shifting laser microscopy (PLM) revealed that the laser phase shift of cells in the G
2
/M phase was markedly higher than that of cells in the G
0
/G
1
phase. Even in the synchronous cultures to G
0
/G
1
and G
2
/M cell cycle phases, the laser phase shift of the cells in the G
2
/M phase was markedly higher than that of the cells in the G
0
/G
1
phase. The analysis of the cultures of MSCs from different donors with the addition of FGF2 at different concentrations revealed that there was a marked negative correlation between the average phase shift and mean generation time. In conclusion, it is possible to estimate noninvasively the proliferation activity of MSCs population by measuring the phase shift using PLM.</description><subject>Antibodies</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Bone marrow</subject><subject>Bromodeoxyuridine</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell proliferation</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Fibroblast growth factor 2</subject><subject>Fibroblasts</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>G1 phase</subject><subject>JAACT Special Issue</subject><subject>Lasers</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchyme</subject><subject>Microscopes</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Penicillin</subject><subject>Phase shift</subject><subject>Process controls</subject><subject>Statistical analysis</subject><subject>Stem cells</subject><subject>Synchronous culture</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9ks-L1TAQx4O4uM-nf4AXCYjopZofTdNcBFn8sbDoRc8hTZPXLG1Sk_ZB7_7hptvHrruglwzJfGYy850B4AVG7zBC_H3CqMJVgZAoBFmPR2CHGacF4rx-DHYovxYCVeIcPE3pGmWQY_oEnGNRCVrVdAd-fwve-aNK7migSZMb1OSCh8FCbfoe6kX3Bo6dSgYq38Ixht5ZEzcqG7Oi3TwoDweTjNfdMqgepskMNxkSbJYtvkids5PzB9jnW4SD0zEkHcblGTizqk_m-cnuwc_Pn35cfC2uvn-5vPh4VWgm6qmwZatYiZHlbWmFbbkhQjeVrURDeClqRCnjCnOLMWXCVKpVrcaNIZXljNSM7sGHLe84N4NptfFTVL0cY-46LjIoJ-97vOvkIRwl4bxkWa89eHNKEMOvOcslB5fWLpU3YU6SU1ozTAXK5Nv_khgJWpZYYJrRVw_Q6zBHn4WQROCaMF7fUHijVtFSNPa2bIzkug1y2waZhyzXbZAix7z8u9-7iNP4M_D6BKikVW-j8tqlW44QwhBiq3Bk41J2-YOJdyX--_c_Nz_Psg</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Tokumitsu, Ayako</creator><creator>Wakitani, Shigeyuki</creator><creator>Takagi, Mutsumi</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090401</creationdate><title>Noninvasive estimation of cell cycle phase and proliferation rate of human mesenchymal stem cells by phase-shifting laser microscopy</title><author>Tokumitsu, Ayako ; Wakitani, Shigeyuki ; Takagi, Mutsumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c598t-f4da5410f7d4f9fd7e29cb6f69b2749803357a17f11359e6adadc1be26f752853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Antibodies</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Bone marrow</topic><topic>Bromodeoxyuridine</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cell proliferation</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Fibroblast growth factor 2</topic><topic>Fibroblasts</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>G1 phase</topic><topic>JAACT Special Issue</topic><topic>Lasers</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchyme</topic><topic>Microscopes</topic><topic>Microscopy</topic><topic>Morphology</topic><topic>Penicillin</topic><topic>Phase shift</topic><topic>Process controls</topic><topic>Statistical analysis</topic><topic>Stem cells</topic><topic>Synchronous culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tokumitsu, Ayako</creatorcontrib><creatorcontrib>Wakitani, Shigeyuki</creatorcontrib><creatorcontrib>Takagi, Mutsumi</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tokumitsu, Ayako</au><au>Wakitani, Shigeyuki</au><au>Takagi, Mutsumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Noninvasive estimation of cell cycle phase and proliferation rate of human mesenchymal stem cells by phase-shifting laser microscopy</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><addtitle>Cytotechnology</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>59</volume><issue>3</issue><spage>161</spage><epage>167</epage><pages>161-167</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>The simultaneous determination of the cell cycle phase of individual adherent mesenchymal stem cells (MSCs) using a fluorescence microscope after staining with 4′,6-diamidine-2′-phenylindole dihydrochloride and bromodeoxyuridine and the laser phase shift by phase-shifting laser microscopy (PLM) revealed that the laser phase shift of cells in the G
2
/M phase was markedly higher than that of cells in the G
0
/G
1
phase. Even in the synchronous cultures to G
0
/G
1
and G
2
/M cell cycle phases, the laser phase shift of the cells in the G
2
/M phase was markedly higher than that of the cells in the G
0
/G
1
phase. The analysis of the cultures of MSCs from different donors with the addition of FGF2 at different concentrations revealed that there was a marked negative correlation between the average phase shift and mean generation time. In conclusion, it is possible to estimate noninvasively the proliferation activity of MSCs population by measuring the phase shift using PLM.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>19693683</pmid><doi>10.1007/s10616-009-9209-9</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0920-9069 |
| ispartof | Cytotechnology (Dordrecht), 2009-04, Vol.59 (3), p.161-167 |
| issn | 0920-9069 1573-0778 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2774568 |
| source | Springer Nature - Complete Springer Journals; EZB-FREE-00999 freely available EZB journals; ProQuest Central UK/Ireland; PubMed Central; ProQuest Central |
| subjects | Antibodies Biochemistry Biological and medical sciences Biomedicine Biotechnology Bone marrow Bromodeoxyuridine Cell culture Cell cycle Cell proliferation Chemistry Chemistry and Materials Science Fibroblast growth factor 2 Fibroblasts Fluorescence Fundamental and applied biological sciences. Psychology G1 phase JAACT Special Issue Lasers Mesenchymal stem cells Mesenchyme Microscopes Microscopy Morphology Penicillin Phase shift Process controls Statistical analysis Stem cells Synchronous culture |
| title | Noninvasive estimation of cell cycle phase and proliferation rate of human mesenchymal stem cells by phase-shifting laser microscopy |
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