Comparison of two methods used for monitoring low-copy cytomegalovirus infection in a patient with chronic myeloid leukemia after unrelated umbilical cord blood transplantation
Introduction: Detection of human cytomegalovirus (CMV, HHV-5) DNA in clinical specimens is considered a cornerstone in the diagnosis of HHV-5 disease. The present study compared two quantitative methods used for diagnosing cytomegalovirus infection in a 21-year-old woman with chronic myeloid leukemi...
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| Veröffentlicht in: | Archivum Immunologiae et Therapiae Experimentalis 2007-06, Vol.55 (3), p.199-203 |
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| creator | Dzieciątkowski, Tomasz Przybylski, Maciej Tomaszewska, Agnieszka Rokicka, Małgorzata Łuczak, Mirosław |
| description | Introduction: Detection of human cytomegalovirus (CMV, HHV-5) DNA in clinical specimens is considered a cornerstone in the diagnosis of HHV-5 disease. The present study compared two quantitative methods used for diagnosing cytomegalovirus infection in a 21-year-old woman with chronic myeloid leukemia after an unrelated umbilical cord blood transplantation. Materials and Methods: Blood samples were tested for the presence of HHV-5 DNA using the LightCycler PCR, the quantitative Eclipse® CMV DNA Detection Kit, and a qualitative in-house PCR assay using primers that amplify part of the HHV-5 MIE gene. Results: Results from samples containing a low cytomegalovirus load were more accurate with the LightCycler test than those obtained with the Eclipse® test, which underestimated the viral load of samples containing low DNA copy numbers. Conclusions: These findings underline the value of novel PCR methods used in current therapeutic procedures and in monitoring antiviral therapy with nucleoside analogs. The high level of sensitivity, specificity, accuracy, and rapidity provided by the LightCycler instrument are favorable for the use of this system in the detection of HHV-5 DNA in clinical specimens. |
| doi_str_mv | 10.1007/s00005-007-0019-5 |
| format | Article |
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The present study compared two quantitative methods used for diagnosing cytomegalovirus infection in a 21-year-old woman with chronic myeloid leukemia after an unrelated umbilical cord blood transplantation. Materials and Methods: Blood samples were tested for the presence of HHV-5 DNA using the LightCycler PCR, the quantitative Eclipse® CMV DNA Detection Kit, and a qualitative in-house PCR assay using primers that amplify part of the HHV-5 MIE gene. Results: Results from samples containing a low cytomegalovirus load were more accurate with the LightCycler test than those obtained with the Eclipse® test, which underestimated the viral load of samples containing low DNA copy numbers. Conclusions: These findings underline the value of novel PCR methods used in current therapeutic procedures and in monitoring antiviral therapy with nucleoside analogs. The high level of sensitivity, specificity, accuracy, and rapidity provided by the LightCycler instrument are favorable for the use of this system in the detection of HHV-5 DNA in clinical specimens.</description><identifier>ISSN: 0004-069X</identifier><identifier>EISSN: 1661-4917</identifier><identifier>DOI: 10.1007/s00005-007-0019-5</identifier><identifier>PMID: 17557148</identifier><language>eng</language><publisher>Switzerland: Basel : Birkhäuser-Verlag</publisher><subject>Adult ; Antiviral agents ; Antiviral Agents - therapeutic use ; Chronic infection ; Chronic myeloid leukemia ; Cord blood ; Cord Blood Stem Cell Transplantation ; Cytomegalovirus ; Cytomegalovirus - isolation & purification ; cytomegalovirus infection ; Cytomegalovirus Infections - blood ; Cytomegalovirus Infections - complications ; Cytomegalovirus Infections - drug therapy ; Cytomegalovirus Infections - virology ; Deoxyribonucleic acid ; DNA ; DNA, Viral - blood ; Female ; Humans ; Leukemia ; Leukemia, Myeloid, Chronic-Phase - blood ; Leukemia, Myeloid, Chronic-Phase - complications ; Leukemia, Myeloid, Chronic-Phase - therapy ; Medical research ; Microbiology ; Myeloid leukemia ; Nucleoside analogs ; Polymerase Chain Reaction ; real-time PCR ; Sensitivity and Specificity ; Short Communication ; Transplantation ; Umbilical cord ; umbilical cord blood transplantation</subject><ispartof>Archivum Immunologiae et Therapiae Experimentalis, 2007-06, Vol.55 (3), p.199-203</ispartof><rights>Birkhäuser Verlag, Basel 2007.</rights><rights>Birkhäuser Verlag, Basel 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-209ad543bec6b0ea7d88e328bdc0c4fc871c53469cc4cff59c60865309ab89dc3</citedby><cites>FETCH-LOGICAL-c507t-209ad543bec6b0ea7d88e328bdc0c4fc871c53469cc4cff59c60865309ab89dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17557148$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dzieciątkowski, Tomasz</creatorcontrib><creatorcontrib>Przybylski, Maciej</creatorcontrib><creatorcontrib>Tomaszewska, Agnieszka</creatorcontrib><creatorcontrib>Rokicka, Małgorzata</creatorcontrib><creatorcontrib>Łuczak, Mirosław</creatorcontrib><title>Comparison of two methods used for monitoring low-copy cytomegalovirus infection in a patient with chronic myeloid leukemia after unrelated umbilical cord blood transplantation</title><title>Archivum Immunologiae et Therapiae Experimentalis</title><addtitle>Arch Immunol Ther Exp (Warsz)</addtitle><description>Introduction: Detection of human cytomegalovirus (CMV, HHV-5) DNA in clinical specimens is considered a cornerstone in the diagnosis of HHV-5 disease. The present study compared two quantitative methods used for diagnosing cytomegalovirus infection in a 21-year-old woman with chronic myeloid leukemia after an unrelated umbilical cord blood transplantation. Materials and Methods: Blood samples were tested for the presence of HHV-5 DNA using the LightCycler PCR, the quantitative Eclipse® CMV DNA Detection Kit, and a qualitative in-house PCR assay using primers that amplify part of the HHV-5 MIE gene. Results: Results from samples containing a low cytomegalovirus load were more accurate with the LightCycler test than those obtained with the Eclipse® test, which underestimated the viral load of samples containing low DNA copy numbers. Conclusions: These findings underline the value of novel PCR methods used in current therapeutic procedures and in monitoring antiviral therapy with nucleoside analogs. The high level of sensitivity, specificity, accuracy, and rapidity provided by the LightCycler instrument are favorable for the use of this system in the detection of HHV-5 DNA in clinical specimens.</description><subject>Adult</subject><subject>Antiviral agents</subject><subject>Antiviral Agents - therapeutic use</subject><subject>Chronic infection</subject><subject>Chronic myeloid leukemia</subject><subject>Cord blood</subject><subject>Cord Blood Stem Cell Transplantation</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus - isolation & purification</subject><subject>cytomegalovirus infection</subject><subject>Cytomegalovirus Infections - blood</subject><subject>Cytomegalovirus Infections - complications</subject><subject>Cytomegalovirus Infections - drug therapy</subject><subject>Cytomegalovirus Infections - virology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Viral - blood</subject><subject>Female</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Leukemia, Myeloid, Chronic-Phase - blood</subject><subject>Leukemia, Myeloid, Chronic-Phase - complications</subject><subject>Leukemia, Myeloid, Chronic-Phase - therapy</subject><subject>Medical research</subject><subject>Microbiology</subject><subject>Myeloid leukemia</subject><subject>Nucleoside analogs</subject><subject>Polymerase Chain Reaction</subject><subject>real-time PCR</subject><subject>Sensitivity and Specificity</subject><subject>Short Communication</subject><subject>Transplantation</subject><subject>Umbilical cord</subject><subject>umbilical cord blood transplantation</subject><issn>0004-069X</issn><issn>1661-4917</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFUtuKFDEQbURxx9UP8EWDgm-tSefW_SLI4K7Cgg-64FtIp9MzWdOpNknvMH_lJ5pxBm8gBkKqqFOnLjlV9ZjglwRj-SrhcnhdzHJJV_M71YoIQWrWEXm3WpUoq7HoPp9VD1K6KR7lhN2vzojkXBLWrqpva5hmHV2CgGBEeQdosnkLQ0JLsgMaIaIJgssQXdggD7vawLxHZp9hshvt4dbFJSEXRmuyKywuII1mnZ0NGe1c3iKzjYXBoGlvPbgBebt8sZPTSI_ZRrSEaL3Opdgy9c47oz0yEAfUe4AB5ahDmr0OWR_4H1b3Ru2TfXR6z6vri7ef1u_qqw-X79dvrmrDscx1gzs9cEZ7a0SPrZZD21ratP1gsGGjaSUxnDLRGcPMOPLOCNwKTkta33aDoefV6yPvvPSTHUyZJmqv5ugmHfcKtFN_RoLbqg3cqkYKLhgpBC9OBBG-LjZlNblkrC-TWFiSkliQ8gnNf4ENJrJ0zgrw2V_AG1hiKFtQrewYZfhH2ef_AjUdpS1um-ZARY4oEyGlaMefgxGsDtpSR22pg3nQluIl58nvG_mVcRJTATw9AkYNSm-KqNT1x9I9xUR0rCWMfgcaptjy</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>Dzieciątkowski, Tomasz</creator><creator>Przybylski, Maciej</creator><creator>Tomaszewska, Agnieszka</creator><creator>Rokicka, Małgorzata</creator><creator>Łuczak, Mirosław</creator><general>Basel : Birkhäuser-Verlag</general><general>Springer Nature B.V</general><general>Birkhäuser-Verlag</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070601</creationdate><title>Comparison of two methods used for monitoring low-copy cytomegalovirus infection in a patient with chronic myeloid leukemia after unrelated umbilical cord blood transplantation</title><author>Dzieciątkowski, Tomasz ; Przybylski, Maciej ; Tomaszewska, Agnieszka ; Rokicka, Małgorzata ; Łuczak, Mirosław</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-209ad543bec6b0ea7d88e328bdc0c4fc871c53469cc4cff59c60865309ab89dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adult</topic><topic>Antiviral agents</topic><topic>Antiviral Agents - therapeutic use</topic><topic>Chronic infection</topic><topic>Chronic myeloid leukemia</topic><topic>Cord blood</topic><topic>Cord Blood Stem Cell Transplantation</topic><topic>Cytomegalovirus</topic><topic>Cytomegalovirus - isolation & purification</topic><topic>cytomegalovirus infection</topic><topic>Cytomegalovirus Infections - blood</topic><topic>Cytomegalovirus Infections - complications</topic><topic>Cytomegalovirus Infections - drug therapy</topic><topic>Cytomegalovirus Infections - virology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Viral - blood</topic><topic>Female</topic><topic>Humans</topic><topic>Leukemia</topic><topic>Leukemia, Myeloid, Chronic-Phase - blood</topic><topic>Leukemia, Myeloid, Chronic-Phase - complications</topic><topic>Leukemia, Myeloid, Chronic-Phase - therapy</topic><topic>Medical research</topic><topic>Microbiology</topic><topic>Myeloid leukemia</topic><topic>Nucleoside analogs</topic><topic>Polymerase Chain Reaction</topic><topic>real-time PCR</topic><topic>Sensitivity and Specificity</topic><topic>Short Communication</topic><topic>Transplantation</topic><topic>Umbilical cord</topic><topic>umbilical cord blood transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dzieciątkowski, Tomasz</creatorcontrib><creatorcontrib>Przybylski, Maciej</creatorcontrib><creatorcontrib>Tomaszewska, Agnieszka</creatorcontrib><creatorcontrib>Rokicka, Małgorzata</creatorcontrib><creatorcontrib>Łuczak, Mirosław</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Archivum Immunologiae et Therapiae Experimentalis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dzieciątkowski, Tomasz</au><au>Przybylski, Maciej</au><au>Tomaszewska, Agnieszka</au><au>Rokicka, Małgorzata</au><au>Łuczak, Mirosław</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of two methods used for monitoring low-copy cytomegalovirus infection in a patient with chronic myeloid leukemia after unrelated umbilical cord blood transplantation</atitle><jtitle>Archivum Immunologiae et Therapiae Experimentalis</jtitle><addtitle>Arch Immunol Ther Exp (Warsz)</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>55</volume><issue>3</issue><spage>199</spage><epage>203</epage><pages>199-203</pages><issn>0004-069X</issn><eissn>1661-4917</eissn><abstract>Introduction: Detection of human cytomegalovirus (CMV, HHV-5) DNA in clinical specimens is considered a cornerstone in the diagnosis of HHV-5 disease. The present study compared two quantitative methods used for diagnosing cytomegalovirus infection in a 21-year-old woman with chronic myeloid leukemia after an unrelated umbilical cord blood transplantation. Materials and Methods: Blood samples were tested for the presence of HHV-5 DNA using the LightCycler PCR, the quantitative Eclipse® CMV DNA Detection Kit, and a qualitative in-house PCR assay using primers that amplify part of the HHV-5 MIE gene. Results: Results from samples containing a low cytomegalovirus load were more accurate with the LightCycler test than those obtained with the Eclipse® test, which underestimated the viral load of samples containing low DNA copy numbers. Conclusions: These findings underline the value of novel PCR methods used in current therapeutic procedures and in monitoring antiviral therapy with nucleoside analogs. The high level of sensitivity, specificity, accuracy, and rapidity provided by the LightCycler instrument are favorable for the use of this system in the detection of HHV-5 DNA in clinical specimens.</abstract><cop>Switzerland</cop><pub>Basel : Birkhäuser-Verlag</pub><pmid>17557148</pmid><doi>10.1007/s00005-007-0019-5</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Antiviral agents Antiviral Agents - therapeutic use Chronic infection Chronic myeloid leukemia Cord blood Cord Blood Stem Cell Transplantation Cytomegalovirus Cytomegalovirus - isolation & purification cytomegalovirus infection Cytomegalovirus Infections - blood Cytomegalovirus Infections - complications Cytomegalovirus Infections - drug therapy Cytomegalovirus Infections - virology Deoxyribonucleic acid DNA DNA, Viral - blood Female Humans Leukemia Leukemia, Myeloid, Chronic-Phase - blood Leukemia, Myeloid, Chronic-Phase - complications Leukemia, Myeloid, Chronic-Phase - therapy Medical research Microbiology Myeloid leukemia Nucleoside analogs Polymerase Chain Reaction real-time PCR Sensitivity and Specificity Short Communication Transplantation Umbilical cord umbilical cord blood transplantation |
| title | Comparison of two methods used for monitoring low-copy cytomegalovirus infection in a patient with chronic myeloid leukemia after unrelated umbilical cord blood transplantation |
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