A new polymorphism in the GRP78 is not associated with HBV invasion

AIM： To examine the association between -86 bp （T 〉 A） in the glucose-regulated protein 78 gene （GRP78） and hepatitis B virus （HBV） invasion. METHODS： DNA was genotyped for the single-nucleotide polymorphism by polymerase chain reaction followed by sequencing in a sample of 382 unrelated HBV carriers and a total of 350 sex- and age-matched healthy controls. Serological markers for HBV infection were determined by enzyme-linked immunosorbent assay kits or clinical chemistry testing. RESULTS： The distributions of allelotype and genotype in cases were not significantly different from those in controls. In addition, our findings suggested that neither alanine aminotransferase/hepatitis B e antigen nor HBV-DNA were associated with the allele/genotype variation in HBV infected individuals.CONCLUSION： -86 bp T 〉 A polymorphism in GRP78 gene is not related to the clinical risk and acute exacerbation of HBV invasion.