Separation of 1-23-kb complementary DNA strands by urea-agarose gel electrophoresis

Double-stranded (ds), as well as denatured, single-stranded (ss) DNA samples can be analyzed on urea-agarose gels. Here we report that after denaturation by heat in the presence of 8 M urea, the two strands of the same ds DNA fragment of ~1-20-kb size migrate differently in 1 M urea containing agaro...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Nucleic acids research 2009-09, Vol.37 (17), p.e112-e112
Hauptverfasser:	Hegedüs, Éva, Kókai, Endre, Kotlyar, Alexander, Dombrádi, Viktor, Szabó, Gábor
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA, Complementary - chemistry DNA, Complementary - isolation & purification DNA, Fungal - chemistry DNA, Fungal - isolation & purification DNA, Single-Stranded - isolation & purification Electrophoresis, Agar Gel - methods Methods Online Nucleic Acid Denaturation Saccharomyces cerevisiae - genetics Urea - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!