Functional requirement of CCN2 for intramembranous bone formation in embryonic mice
CCN2 is best known as a promoter of chondrocyte differentiation among the CCN family members, and Ccn2 null mutant mice display skeletal dysmorphisms. However, little is known concerning the roles of CCN2 during bone formation. We herein present a comparative analysis of wild-type and Ccn2 null mice...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2008-02, Vol.366 (2), p.450-456 |
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| creator | Kawaki, Harumi Kubota, Satoshi Suzuki, Akiko Yamada, Tomohiro Matsumura, Tatsushi Mandai, Toshiko Yao, Mayumi Maeda, Takeyasu Lyons, Karen M. Takigawa, Masaharu |
| description | CCN2 is best known as a promoter of chondrocyte differentiation among the CCN family members, and Ccn2 null mutant mice display skeletal dysmorphisms. However, little is known concerning the roles of CCN2 during bone formation. We herein present a comparative analysis of wild-type and Ccn2 null mice to investigate the roles of CCN2 in bone development. Multiple histochemical methods were employed to analyze the effects of CCN2 deletion in vivo, and effects of CCN2 on the osteogenic response were evaluated with the isolated and cultured osteoblasts. As a result, we found a drastic reduction of the osteoblastic phenotype in Ccn2 null mutants. Importantly, addition of exogenous CCN2 promoted every step of osteoblast differentiation and rescued the attenuated activities of the Ccn2 null osteoblasts. These results suggest that CCN2 is required not only for the regulation of cartilage and subsequent events, but also for the normal intramembranous bone development. |
| doi_str_mv | 10.1016/j.bbrc.2007.11.155 |
| format | Article |
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These results suggest that CCN2 is required not only for the regulation of cartilage and subsequent events, but also for the normal intramembranous bone development.</description><subject>Animals</subject><subject>CCN family</subject><subject>Cells, Cultured</subject><subject>Connective Tissue Growth Factor</subject><subject>Differentiation</subject><subject>Immediate-Early Proteins - metabolism</subject><subject>Intercellular Signaling Peptides and Proteins - metabolism</subject><subject>Intramembranous bone formation</subject><subject>Mice - embryology</subject><subject>Mice - metabolism</subject><subject>Null mutant</subject><subject>Osteoblast</subject><subject>Osteoblasts - physiology</subject><subject>Osteogenesis - physiology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuL1TAUx4Mozp3RL-BCunLXek5eTUEEucw4wqALFdyFNE00lzaZSduB-fam3IuPja7O4v_gnPMj5AVCg4Dy9aHp-2wbCtA2iA0K8YjsEDqoKQJ_THYAIGva4bczcj7PBwBELrun5AwVyFaJbkc-X63RLiFFM1bZ3a0hu8nFpUq-2u8_0sqnXIW4ZDO5qc8mpnWu-hTdJkxmCxa52rSHFIOtpmDdM_LEm3F2z0_zgny9uvyyv65vPr3_sH93U1shYKlpK5xSvgUPnHHWs456ZEpxBONb1XLBheoGJRgwL2CQnILiynSUcWqRsgvy9th7u_aTG6zb9hz1bQ6TyQ86maD_VmL4ob-ne01bCaKTpeDVqSCnu9XNi57CbN04mujKoboFVJwx-l8jBSkkZ20x0qPR5jTP2flf2yDoDZo-6A2a3qBpRF2gldDLP-_4HTlRKoY3R4Mr37wPLuvZBhetGwouu-ghhX_1_wRAGafc</recordid><startdate>20080208</startdate><enddate>20080208</enddate><creator>Kawaki, Harumi</creator><creator>Kubota, Satoshi</creator><creator>Suzuki, Akiko</creator><creator>Yamada, Tomohiro</creator><creator>Matsumura, Tatsushi</creator><creator>Mandai, Toshiko</creator><creator>Yao, Mayumi</creator><creator>Maeda, Takeyasu</creator><creator>Lyons, Karen M.</creator><creator>Takigawa, Masaharu</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080208</creationdate><title>Functional requirement of CCN2 for intramembranous bone formation in embryonic mice</title><author>Kawaki, Harumi ; 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| subjects | Animals CCN family Cells, Cultured Connective Tissue Growth Factor Differentiation Immediate-Early Proteins - metabolism Intercellular Signaling Peptides and Proteins - metabolism Intramembranous bone formation Mice - embryology Mice - metabolism Null mutant Osteoblast Osteoblasts - physiology Osteogenesis - physiology |
| title | Functional requirement of CCN2 for intramembranous bone formation in embryonic mice |
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