Comparative Analysis of Expressed Sequence Tag (EST) Libraries in the Seagrass Zostera marina Subjected to Temperature Stress
Global warming is associated with increasing stress and mortality on temperate seagrass beds, in particular during periods of high sea surface temperatures during summer months, adding to existing anthropogenic impacts, such as eutrophication and habitat destruction. We compare several expressed seq...
Gespeichert in:
| Veröffentlicht in: | Marine Biotechnology 2008-05, Vol.10 (3), p.297-309 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 309 |
|---|---|
| container_issue | 3 |
| container_start_page | 297 |
| container_title | Marine Biotechnology |
| container_volume | 10 |
| creator | Reusch, Thorsten B. H. Veron, Amelie S. Preuss, Christoph Weiner, January Wissler, Lothar Beck, Alfred Klages, Sven Kube, Michael Reinhardt, Richard Bornberg-Bauer, Erich |
| description | Global warming is associated with increasing stress and mortality on temperate seagrass beds, in particular during periods of high sea surface temperatures during summer months, adding to existing anthropogenic impacts, such as eutrophication and habitat destruction. We compare several expressed sequence tag (EST) in the ecologically important seagrass
Zostera marina
(eelgrass) to elucidate the molecular genetic basis of adaptation to environmental extremes. We compared the tentative unigene (TUG) frequencies of libraries derived from leaf and meristematic tissue from a control situation with two experimentally imposed temperature stress conditions and found that TUG composition is markedly different among these conditions (all
P
|
| doi_str_mv | 10.1007/s10126-007-9065-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2757623</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1898936331</sourcerecordid><originalsourceid>FETCH-LOGICAL-c598t-521cdbfd6cfe78161d77ed185f16d671922f5bbb98ed8b5acf795400eddeebee3</originalsourceid><addsrcrecordid>eNp1ksGO0zAQhi0EYpfCA3BBFgfEHgK209jJBamqCotUiUPLhYvlxJM2VRIH26nYA-_ORKl2YSVOHvn_5h97Zgh5zdkHzpj6GDjjQiYYJgWTWSKfkGu-TGUiRCqf3scivyIvQjgxBFXKnpMrnou0KKS4Jr_XrhuMN7E5A131pr0LTaCupptfg4cQwNId_Byhr4DuzYG-3-z2N3TblN74BgJtehqPgIw5eBMC_eFCBG9oh3Jv6G4sT1BFdImO7qEbUIujx4Q4ub8kz2rTBnh1ORfk--fNfn2bbL99-bpebZMqK_KYZIJXtqytrGpQOZfcKgWW51nNpZWKF0LUWVmWRQ42LzNT1arIloyBtQAlQLogn2bfYSw7sBX00ZtWD77Bd95pZxr9r9I3R31wZy1UpqRI0eBmNjg-SrtdbfV0x0TKFHb9zJF9dynmHXYuRN01oYK2NT24MWjBUwSXEsG3j8CTGz3OABmWFzLn6LggfIYq70LwUN-X50xPW6DnLdBTOG2Bnozf_P3dh4zL2BEQMxBQ6g_gHyr_3_UPXIe_LQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>208968172</pqid></control><display><type>article</type><title>Comparative Analysis of Expressed Sequence Tag (EST) Libraries in the Seagrass Zostera marina Subjected to Temperature Stress</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Reusch, Thorsten B. H. ; Veron, Amelie S. ; Preuss, Christoph ; Weiner, January ; Wissler, Lothar ; Beck, Alfred ; Klages, Sven ; Kube, Michael ; Reinhardt, Richard ; Bornberg-Bauer, Erich</creator><creatorcontrib>Reusch, Thorsten B. H. ; Veron, Amelie S. ; Preuss, Christoph ; Weiner, January ; Wissler, Lothar ; Beck, Alfred ; Klages, Sven ; Kube, Michael ; Reinhardt, Richard ; Bornberg-Bauer, Erich</creatorcontrib><description>Global warming is associated with increasing stress and mortality on temperate seagrass beds, in particular during periods of high sea surface temperatures during summer months, adding to existing anthropogenic impacts, such as eutrophication and habitat destruction. We compare several expressed sequence tag (EST) in the ecologically important seagrass
Zostera marina
(eelgrass) to elucidate the molecular genetic basis of adaptation to environmental extremes. We compared the tentative unigene (TUG) frequencies of libraries derived from leaf and meristematic tissue from a control situation with two experimentally imposed temperature stress conditions and found that TUG composition is markedly different among these conditions (all
P
< 0.0001). Under heat stress, we find that 63 TUGs are differentially expressed (d.e.) at 25°C compared with lower, no-stress condition temperatures (4°C and 17°C). Approximately one-third of d.e. eelgrass genes were characteristic for the stress response of the terrestrial plant model
Arabidopsis thaliana
. The changes in gene expression suggest complex photosynthetic adjustments among light-harvesting complexes, reaction center subunits of photosystem I and II, and components of the dark reaction. Heat shock encoding proteins and reactive oxygen scavengers also were identified, but their overall frequency was too low to perform statistical tests. In all conditions, the most abundant transcript (3–15%) was a putative metallothionein gene with unknown function. We also find evidence that heat stress may translate to enhanced infection by protists. A total of 210 TUGs contain one or more microsatellites as potential candidates for gene-linked genetic markers. Data are publicly available in a user-friendly database at
http://www.uni-muenster.de/Evolution/ebb/Services/zostera
.</description><identifier>ISSN: 1436-2228</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s10126-007-9065-6</identifier><identifier>PMID: 18239962</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Anthropogenic factors ; Arabidopsis - genetics ; Arabidopsis thaliana ; Biodiversity ; Biomedical and Life Sciences ; Biotechnology ; Climate change ; Comparative analysis ; Down-Regulation ; Engineering ; Environmental degradation ; Eutrophication ; Evolution ; Expressed Sequence Tags ; Freshwater & Marine Ecology ; Gene expression ; Gene Expression Regulation, Plant ; Gene Library ; Genetic markers ; Genetic Variation ; Genomics ; Global warming ; Grasses ; Greenhouse Effect ; Habitat loss ; Heat ; Heat tolerance ; Heat-Shock Proteins - genetics ; Hot Temperature ; Life Sciences ; Marine ; Microbiology ; Microsatellite Repeats - genetics ; Open Reading Frames - genetics ; Original ; Original Article ; Plant tissues ; Sea surface temperature ; Studies ; Temperature ; Up-Regulation ; Zoology ; Zostera ; Zostera marina ; Zosteraceae - genetics</subject><ispartof>Marine Biotechnology, 2008-05, Vol.10 (3), p.297-309</ispartof><rights>Springer Science+Business Media, LLC 2007</rights><rights>Springer Science+Business Media, LLC 2008</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c598t-521cdbfd6cfe78161d77ed185f16d671922f5bbb98ed8b5acf795400eddeebee3</citedby><cites>FETCH-LOGICAL-c598t-521cdbfd6cfe78161d77ed185f16d671922f5bbb98ed8b5acf795400eddeebee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10126-007-9065-6$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10126-007-9065-6$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18239962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://univ-lyon1.hal.science/hal-02307236$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Reusch, Thorsten B. H.</creatorcontrib><creatorcontrib>Veron, Amelie S.</creatorcontrib><creatorcontrib>Preuss, Christoph</creatorcontrib><creatorcontrib>Weiner, January</creatorcontrib><creatorcontrib>Wissler, Lothar</creatorcontrib><creatorcontrib>Beck, Alfred</creatorcontrib><creatorcontrib>Klages, Sven</creatorcontrib><creatorcontrib>Kube, Michael</creatorcontrib><creatorcontrib>Reinhardt, Richard</creatorcontrib><creatorcontrib>Bornberg-Bauer, Erich</creatorcontrib><title>Comparative Analysis of Expressed Sequence Tag (EST) Libraries in the Seagrass Zostera marina Subjected to Temperature Stress</title><title>Marine Biotechnology</title><addtitle>Mar Biotechnol</addtitle><addtitle>Mar Biotechnol (NY)</addtitle><description>Global warming is associated with increasing stress and mortality on temperate seagrass beds, in particular during periods of high sea surface temperatures during summer months, adding to existing anthropogenic impacts, such as eutrophication and habitat destruction. We compare several expressed sequence tag (EST) in the ecologically important seagrass
Zostera marina
(eelgrass) to elucidate the molecular genetic basis of adaptation to environmental extremes. We compared the tentative unigene (TUG) frequencies of libraries derived from leaf and meristematic tissue from a control situation with two experimentally imposed temperature stress conditions and found that TUG composition is markedly different among these conditions (all
P
< 0.0001). Under heat stress, we find that 63 TUGs are differentially expressed (d.e.) at 25°C compared with lower, no-stress condition temperatures (4°C and 17°C). Approximately one-third of d.e. eelgrass genes were characteristic for the stress response of the terrestrial plant model
Arabidopsis thaliana
. The changes in gene expression suggest complex photosynthetic adjustments among light-harvesting complexes, reaction center subunits of photosystem I and II, and components of the dark reaction. Heat shock encoding proteins and reactive oxygen scavengers also were identified, but their overall frequency was too low to perform statistical tests. In all conditions, the most abundant transcript (3–15%) was a putative metallothionein gene with unknown function. We also find evidence that heat stress may translate to enhanced infection by protists. A total of 210 TUGs contain one or more microsatellites as potential candidates for gene-linked genetic markers. Data are publicly available in a user-friendly database at
http://www.uni-muenster.de/Evolution/ebb/Services/zostera
.</description><subject>Anthropogenic factors</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis thaliana</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Climate change</subject><subject>Comparative analysis</subject><subject>Down-Regulation</subject><subject>Engineering</subject><subject>Environmental degradation</subject><subject>Eutrophication</subject><subject>Evolution</subject><subject>Expressed Sequence Tags</subject><subject>Freshwater & Marine Ecology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Library</subject><subject>Genetic markers</subject><subject>Genetic Variation</subject><subject>Genomics</subject><subject>Global warming</subject><subject>Grasses</subject><subject>Greenhouse Effect</subject><subject>Habitat loss</subject><subject>Heat</subject><subject>Heat tolerance</subject><subject>Heat-Shock Proteins - genetics</subject><subject>Hot Temperature</subject><subject>Life Sciences</subject><subject>Marine</subject><subject>Microbiology</subject><subject>Microsatellite Repeats - genetics</subject><subject>Open Reading Frames - genetics</subject><subject>Original</subject><subject>Original Article</subject><subject>Plant tissues</subject><subject>Sea surface temperature</subject><subject>Studies</subject><subject>Temperature</subject><subject>Up-Regulation</subject><subject>Zoology</subject><subject>Zostera</subject><subject>Zostera marina</subject><subject>Zosteraceae - genetics</subject><issn>1436-2228</issn><issn>1436-2236</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1ksGO0zAQhi0EYpfCA3BBFgfEHgK209jJBamqCotUiUPLhYvlxJM2VRIH26nYA-_ORKl2YSVOHvn_5h97Zgh5zdkHzpj6GDjjQiYYJgWTWSKfkGu-TGUiRCqf3scivyIvQjgxBFXKnpMrnou0KKS4Jr_XrhuMN7E5A131pr0LTaCupptfg4cQwNId_Byhr4DuzYG-3-z2N3TblN74BgJtehqPgIw5eBMC_eFCBG9oh3Jv6G4sT1BFdImO7qEbUIujx4Q4ub8kz2rTBnh1ORfk--fNfn2bbL99-bpebZMqK_KYZIJXtqytrGpQOZfcKgWW51nNpZWKF0LUWVmWRQ42LzNT1arIloyBtQAlQLogn2bfYSw7sBX00ZtWD77Bd95pZxr9r9I3R31wZy1UpqRI0eBmNjg-SrtdbfV0x0TKFHb9zJF9dynmHXYuRN01oYK2NT24MWjBUwSXEsG3j8CTGz3OABmWFzLn6LggfIYq70LwUN-X50xPW6DnLdBTOG2Bnozf_P3dh4zL2BEQMxBQ6g_gHyr_3_UPXIe_LQ</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Reusch, Thorsten B. H.</creator><creator>Veron, Amelie S.</creator><creator>Preuss, Christoph</creator><creator>Weiner, January</creator><creator>Wissler, Lothar</creator><creator>Beck, Alfred</creator><creator>Klages, Sven</creator><creator>Kube, Michael</creator><creator>Reinhardt, Richard</creator><creator>Bornberg-Bauer, Erich</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7TN</scope><scope>7U9</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>L.F</scope><scope>L.G</scope><scope>L6V</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PCBAR</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7TV</scope><scope>H97</scope><scope>1XC</scope><scope>5PM</scope></search><sort><creationdate>20080501</creationdate><title>Comparative Analysis of Expressed Sequence Tag (EST) Libraries in the Seagrass Zostera marina Subjected to Temperature Stress</title><author>Reusch, Thorsten B. H. ; Veron, Amelie S. ; Preuss, Christoph ; Weiner, January ; Wissler, Lothar ; Beck, Alfred ; Klages, Sven ; Kube, Michael ; Reinhardt, Richard ; Bornberg-Bauer, Erich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c598t-521cdbfd6cfe78161d77ed185f16d671922f5bbb98ed8b5acf795400eddeebee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Anthropogenic factors</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis thaliana</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Climate change</topic><topic>Comparative analysis</topic><topic>Down-Regulation</topic><topic>Engineering</topic><topic>Environmental degradation</topic><topic>Eutrophication</topic><topic>Evolution</topic><topic>Expressed Sequence Tags</topic><topic>Freshwater & Marine Ecology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Library</topic><topic>Genetic markers</topic><topic>Genetic Variation</topic><topic>Genomics</topic><topic>Global warming</topic><topic>Grasses</topic><topic>Greenhouse Effect</topic><topic>Habitat loss</topic><topic>Heat</topic><topic>Heat tolerance</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Hot Temperature</topic><topic>Life Sciences</topic><topic>Marine</topic><topic>Microbiology</topic><topic>Microsatellite Repeats - genetics</topic><topic>Open Reading Frames - genetics</topic><topic>Original</topic><topic>Original Article</topic><topic>Plant tissues</topic><topic>Sea surface temperature</topic><topic>Studies</topic><topic>Temperature</topic><topic>Up-Regulation</topic><topic>Zoology</topic><topic>Zostera</topic><topic>Zostera marina</topic><topic>Zosteraceae - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reusch, Thorsten B. H.</creatorcontrib><creatorcontrib>Veron, Amelie S.</creatorcontrib><creatorcontrib>Preuss, Christoph</creatorcontrib><creatorcontrib>Weiner, January</creatorcontrib><creatorcontrib>Wissler, Lothar</creatorcontrib><creatorcontrib>Beck, Alfred</creatorcontrib><creatorcontrib>Klages, Sven</creatorcontrib><creatorcontrib>Kube, Michael</creatorcontrib><creatorcontrib>Reinhardt, Richard</creatorcontrib><creatorcontrib>Bornberg-Bauer, Erich</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>Pollution Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Marine Biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reusch, Thorsten B. H.</au><au>Veron, Amelie S.</au><au>Preuss, Christoph</au><au>Weiner, January</au><au>Wissler, Lothar</au><au>Beck, Alfred</au><au>Klages, Sven</au><au>Kube, Michael</au><au>Reinhardt, Richard</au><au>Bornberg-Bauer, Erich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Analysis of Expressed Sequence Tag (EST) Libraries in the Seagrass Zostera marina Subjected to Temperature Stress</atitle><jtitle>Marine Biotechnology</jtitle><stitle>Mar Biotechnol</stitle><addtitle>Mar Biotechnol (NY)</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>10</volume><issue>3</issue><spage>297</spage><epage>309</epage><pages>297-309</pages><issn>1436-2228</issn><eissn>1436-2236</eissn><abstract>Global warming is associated with increasing stress and mortality on temperate seagrass beds, in particular during periods of high sea surface temperatures during summer months, adding to existing anthropogenic impacts, such as eutrophication and habitat destruction. We compare several expressed sequence tag (EST) in the ecologically important seagrass
Zostera marina
(eelgrass) to elucidate the molecular genetic basis of adaptation to environmental extremes. We compared the tentative unigene (TUG) frequencies of libraries derived from leaf and meristematic tissue from a control situation with two experimentally imposed temperature stress conditions and found that TUG composition is markedly different among these conditions (all
P
< 0.0001). Under heat stress, we find that 63 TUGs are differentially expressed (d.e.) at 25°C compared with lower, no-stress condition temperatures (4°C and 17°C). Approximately one-third of d.e. eelgrass genes were characteristic for the stress response of the terrestrial plant model
Arabidopsis thaliana
. The changes in gene expression suggest complex photosynthetic adjustments among light-harvesting complexes, reaction center subunits of photosystem I and II, and components of the dark reaction. Heat shock encoding proteins and reactive oxygen scavengers also were identified, but their overall frequency was too low to perform statistical tests. In all conditions, the most abundant transcript (3–15%) was a putative metallothionein gene with unknown function. We also find evidence that heat stress may translate to enhanced infection by protists. A total of 210 TUGs contain one or more microsatellites as potential candidates for gene-linked genetic markers. Data are publicly available in a user-friendly database at
http://www.uni-muenster.de/Evolution/ebb/Services/zostera
.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>18239962</pmid><doi>10.1007/s10126-007-9065-6</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1436-2228 |
| ispartof | Marine Biotechnology, 2008-05, Vol.10 (3), p.297-309 |
| issn | 1436-2228 1436-2236 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2757623 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Anthropogenic factors Arabidopsis - genetics Arabidopsis thaliana Biodiversity Biomedical and Life Sciences Biotechnology Climate change Comparative analysis Down-Regulation Engineering Environmental degradation Eutrophication Evolution Expressed Sequence Tags Freshwater & Marine Ecology Gene expression Gene Expression Regulation, Plant Gene Library Genetic markers Genetic Variation Genomics Global warming Grasses Greenhouse Effect Habitat loss Heat Heat tolerance Heat-Shock Proteins - genetics Hot Temperature Life Sciences Marine Microbiology Microsatellite Repeats - genetics Open Reading Frames - genetics Original Original Article Plant tissues Sea surface temperature Studies Temperature Up-Regulation Zoology Zostera Zostera marina Zosteraceae - genetics |
| title | Comparative Analysis of Expressed Sequence Tag (EST) Libraries in the Seagrass Zostera marina Subjected to Temperature Stress |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T17%3A40%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20Analysis%20of%20Expressed%20Sequence%20Tag%20(EST)%20Libraries%20in%20the%20Seagrass%20Zostera%20marina%20Subjected%20to%20Temperature%20Stress&rft.jtitle=Marine%20Biotechnology&rft.au=Reusch,%20Thorsten%20B.%20H.&rft.date=2008-05-01&rft.volume=10&rft.issue=3&rft.spage=297&rft.epage=309&rft.pages=297-309&rft.issn=1436-2228&rft.eissn=1436-2236&rft_id=info:doi/10.1007/s10126-007-9065-6&rft_dat=%3Cproquest_pubme%3E1898936331%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=208968172&rft_id=info:pmid/18239962&rfr_iscdi=true |