Phosphorylation of FAR1 in response to alpha-factor: a possible requirement for cell-cycle arrest
Exposure of yeast a cells to alpha-factor causes cells to arrest in the G1 phase of the cell cycle. The FAR1 gene is required for this cell-cycle arrest; its product is necessary for the inhibition of a G1 cyclin, CLN2. Earlier work demonstrated that alpha-factor caused an increase in the transcript...
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| Veröffentlicht in: | Molecular biology of the cell 1992-04, Vol.3 (4), p.445-450 |
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| creator | Chang, F Herskowitz, I |
| description | Exposure of yeast a cells to alpha-factor causes cells to arrest in the G1 phase of the cell cycle. The FAR1 gene is required for this cell-cycle arrest; its product is necessary for the inhibition of a G1 cyclin, CLN2. Earlier work demonstrated that alpha-factor caused an increase in the transcription of FAR1 severalfold over a measurable basal level. We now show that transcriptional induction of FAR1 from a heterologous promoter is not sufficient to inhibit CLN2 in the absence of alpha-factor. We also show that FAR1 is phosphorylated in response to alpha-factor and propose that this phosphorylation may be required for FAR1 activity. |
| doi_str_mv | 10.1091/mbc.3.4.445 |
| format | Article |
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The FAR1 gene is required for this cell-cycle arrest; its product is necessary for the inhibition of a G1 cyclin, CLN2. Earlier work demonstrated that alpha-factor caused an increase in the transcription of FAR1 severalfold over a measurable basal level. We now show that transcriptional induction of FAR1 from a heterologous promoter is not sufficient to inhibit CLN2 in the absence of alpha-factor. We also show that FAR1 is phosphorylated in response to alpha-factor and propose that this phosphorylation may be required for FAR1 activity.</description><identifier>ISSN: 1059-1524</identifier><identifier>EISSN: 1939-4586</identifier><identifier>DOI: 10.1091/mbc.3.4.445</identifier><identifier>PMID: 1498364</identifier><language>eng</language><publisher>United States</publisher><subject>Base Sequence ; Cell Cycle - drug effects ; Cell Cycle - physiology ; Fungal Proteins - metabolism ; Gene Expression Regulation, Fungal ; Genes, Fungal ; Mating Factor ; Molecular Sequence Data ; Peptides - pharmacology ; Pheromones - pharmacology ; Phosphorylation ; Promoter Regions, Genetic ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - physiology ; Transcription, Genetic</subject><ispartof>Molecular biology of the cell, 1992-04, Vol.3 (4), p.445-450</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-53e23c1c0d3c8cab5a997f6f62417453039cb5219fea8e8150c91b54700d65733</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC275594/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC275594/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1498364$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, F</creatorcontrib><creatorcontrib>Herskowitz, I</creatorcontrib><title>Phosphorylation of FAR1 in response to alpha-factor: a possible requirement for cell-cycle arrest</title><title>Molecular biology of the cell</title><addtitle>Mol Biol Cell</addtitle><description>Exposure of yeast a cells to alpha-factor causes cells to arrest in the G1 phase of the cell cycle. 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We also show that FAR1 is phosphorylated in response to alpha-factor and propose that this phosphorylation may be required for FAR1 activity.</description><subject>Base Sequence</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Cycle - physiology</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes, Fungal</subject><subject>Mating Factor</subject><subject>Molecular Sequence Data</subject><subject>Peptides - pharmacology</subject><subject>Pheromones - pharmacology</subject><subject>Phosphorylation</subject><subject>Promoter Regions, Genetic</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - physiology</subject><subject>Transcription, Genetic</subject><issn>1059-1524</issn><issn>1939-4586</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkctLxDAQxoMo6_o4eRZy8iKtSfNqBA_L4qqwoIieQ5pNbaVtukkr7H9vll18nGZgfjPzzXwAXGCUYiTxTVuYlKQ0pZQdgCmWRCaU5fww5ojJBLOMHoOTED4RwpRyMQETTGVOOJ0C_VK50FfObxo91K6DroSL2SuGdQe9Db3rgoWDg7rpK52U2gzO30INexdCXTQ2Quux9ra13QBL56GxTZOYjYkl7eOE4QwclboJ9nwfT8H74v5t_pgsnx-e5rNlYohgQ8KIzYjBBq2IyY0umJZSlLzkGcWCMoKINAXLsCytzm2OGTISF4wKhFacCUJOwd1ubj8WrV2ZKMjrRvW-brXfKKdr9b_S1ZX6cF8qE4xJGvuv9v3erccoXLV12F6jO-vGoATBWEjOI3i9A42PT_C2_NmBkdoaoqIhiiiqoiGRvvwr65fdOUC-AXtmiCw</recordid><startdate>19920401</startdate><enddate>19920401</enddate><creator>Chang, F</creator><creator>Herskowitz, I</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19920401</creationdate><title>Phosphorylation of FAR1 in response to alpha-factor: a possible requirement for cell-cycle arrest</title><author>Chang, F ; Herskowitz, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-53e23c1c0d3c8cab5a997f6f62417453039cb5219fea8e8150c91b54700d65733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Base Sequence</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Cycle - physiology</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes, Fungal</topic><topic>Mating Factor</topic><topic>Molecular Sequence Data</topic><topic>Peptides - pharmacology</topic><topic>Pheromones - pharmacology</topic><topic>Phosphorylation</topic><topic>Promoter Regions, Genetic</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - physiology</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, F</creatorcontrib><creatorcontrib>Herskowitz, I</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, F</au><au>Herskowitz, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphorylation of FAR1 in response to alpha-factor: a possible requirement for cell-cycle arrest</atitle><jtitle>Molecular biology of the cell</jtitle><addtitle>Mol Biol Cell</addtitle><date>1992-04-01</date><risdate>1992</risdate><volume>3</volume><issue>4</issue><spage>445</spage><epage>450</epage><pages>445-450</pages><issn>1059-1524</issn><eissn>1939-4586</eissn><abstract>Exposure of yeast a cells to alpha-factor causes cells to arrest in the G1 phase of the cell cycle. The FAR1 gene is required for this cell-cycle arrest; its product is necessary for the inhibition of a G1 cyclin, CLN2. Earlier work demonstrated that alpha-factor caused an increase in the transcription of FAR1 severalfold over a measurable basal level. We now show that transcriptional induction of FAR1 from a heterologous promoter is not sufficient to inhibit CLN2 in the absence of alpha-factor. We also show that FAR1 is phosphorylated in response to alpha-factor and propose that this phosphorylation may be required for FAR1 activity.</abstract><cop>United States</cop><pmid>1498364</pmid><doi>10.1091/mbc.3.4.445</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular biology of the cell, 1992-04, Vol.3 (4), p.445-450 |
| issn | 1059-1524 1939-4586 |
| language | eng |
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| subjects | Base Sequence Cell Cycle - drug effects Cell Cycle - physiology Fungal Proteins - metabolism Gene Expression Regulation, Fungal Genes, Fungal Mating Factor Molecular Sequence Data Peptides - pharmacology Pheromones - pharmacology Phosphorylation Promoter Regions, Genetic Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - physiology Transcription, Genetic |
| title | Phosphorylation of FAR1 in response to alpha-factor: a possible requirement for cell-cycle arrest |
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