Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni
A partial cDNA sequence was obtained from the human blood fluke, Schistosoma mansoni using a signal sequence trap approach. The full-length cDNA was cloned and termed Sm-7TM. The corresponding open reading frame had 7 membrane spanning domains and shared identity with a small, novel group of seven t...
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| Veröffentlicht in: | Parasitology 2007-12, Vol.134 (14), p.2001-2008 |
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| container_issue | 14 |
| container_start_page | 2001 |
| container_title | Parasitology |
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| creator | PEARSON, M. S. McMANUS, D. P. SMYTH, D. J. JONES, M. K. SYKES, A. M. LOUKAS, A. |
| description | A partial cDNA sequence was obtained from the human blood fluke, Schistosoma mansoni using a signal sequence trap approach. The full-length cDNA was cloned and termed Sm-7TM. The corresponding open reading frame had 7 membrane spanning domains and shared identity with a small, novel group of seven transmembrane (7TM) receptors from vertebrates and invertebrates, including the human ee3 receptor – a heptahelical protein implicated in neuronal signalling. Phylogenetic analysis of this novel family showed that the Sm-7TM ORF formed a clade with exclusively invertebrate sequences. Based on topology modelling with ee3, Sm-7TM was predicted to possess an intracellular C-terminal tail, which was expressed as a soluble thioredoxin fusion protein (Sm-7TMC) in Escherichia coli and purified using metal ion-affinity chromatography. A polyclonal antiserum against this domain was used to detect Sm-7TM in detergent-soluble parasite extracts and to immunolocalize the receptor to the tegument of adult S. mansoni. |
| doi_str_mv | 10.1017/S0031182007003393 |
| format | Article |
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Based on topology modelling with ee3, Sm-7TM was predicted to possess an intracellular C-terminal tail, which was expressed as a soluble thioredoxin fusion protein (Sm-7TMC) in Escherichia coli and purified using metal ion-affinity chromatography. A polyclonal antiserum against this domain was used to detect Sm-7TM in detergent-soluble parasite extracts and to immunolocalize the receptor to the tegument of adult S. mansoni.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182007003393</identifier><identifier>PMID: 17714602</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Amino Acid Sequence ; Animals ; Biological and medical sciences ; Cloning, Molecular ; DNA, Complementary ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. 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Models ; Helminth Proteins - chemistry ; Helminth Proteins - genetics ; Helminth Proteins - immunology ; Helminth Proteins - metabolism ; Invertebrates ; Molecular Sequence Data ; Phylogeny ; Protein Structure, Tertiary ; Receptors, Cell Surface - chemistry ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - immunology ; Receptors, Cell Surface - metabolism ; Schistosoma mansoni ; Schistosoma mansoni - genetics ; Schistosoma mansoni - metabolism ; seven transmembrane receptor ; tegument</subject><ispartof>Parasitology, 2007-12, Vol.134 (14), p.2001-2008</ispartof><rights>Copyright © Cambridge University Press 2007</rights><rights>2008 INIST-CNRS</rights><rights>2007 Cambridge University Press 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-eff668d5a658a3139acd50bae56c9d76a44eed0392955a042af0b352e4e6868a3</citedby><cites>FETCH-LOGICAL-c509t-eff668d5a658a3139acd50bae56c9d76a44eed0392955a042af0b352e4e6868a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182007003393/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,230,314,776,780,881,27901,27902,55603</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19862568$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17714602$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PEARSON, M. S.</creatorcontrib><creatorcontrib>McMANUS, D. P.</creatorcontrib><creatorcontrib>SMYTH, D. J.</creatorcontrib><creatorcontrib>JONES, M. K.</creatorcontrib><creatorcontrib>SYKES, A. M.</creatorcontrib><creatorcontrib>LOUKAS, A.</creatorcontrib><title>Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>A partial cDNA sequence was obtained from the human blood fluke, Schistosoma mansoni using a signal sequence trap approach. The full-length cDNA was cloned and termed Sm-7TM. The corresponding open reading frame had 7 membrane spanning domains and shared identity with a small, novel group of seven transmembrane (7TM) receptors from vertebrates and invertebrates, including the human ee3 receptor – a heptahelical protein implicated in neuronal signalling. Phylogenetic analysis of this novel family showed that the Sm-7TM ORF formed a clade with exclusively invertebrate sequences. Based on topology modelling with ee3, Sm-7TM was predicted to possess an intracellular C-terminal tail, which was expressed as a soluble thioredoxin fusion protein (Sm-7TMC) in Escherichia coli and purified using metal ion-affinity chromatography. A polyclonal antiserum against this domain was used to detect Sm-7TM in detergent-soluble parasite extracts and to immunolocalize the receptor to the tegument of adult S. mansoni.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Helminth Proteins - chemistry</subject><subject>Helminth Proteins - genetics</subject><subject>Helminth Proteins - immunology</subject><subject>Helminth Proteins - metabolism</subject><subject>Invertebrates</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Protein Structure, Tertiary</subject><subject>Receptors, Cell Surface - chemistry</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - immunology</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - genetics</subject><subject>Schistosoma mansoni - metabolism</subject><subject>seven transmembrane receptor</subject><subject>tegument</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFv1DAQhS1ERZfCD-CCcoFbwI5jx74goVVpkSqhagtXa9aZ7Lok8WJnK-DXM6uNWhBST2P5fTN-nsfYK8HfCS6a9yvOpRCm4ryhk7TyCVuIWtvSCC2essVBLg_6KXue8y3nXEtdPWOnommI49WCuWUfxzBuChjbwm8hgZ8whd8whTgWsaP7IqbdlkrGOxyLKcGYBxzWVLFI6HE3xVR0KQ7Fym9DnmKOAxQDYTT5BTvpoM_4cq5n7Oun85vlZXn15eLz8uNV6RW3U4ldp7VpFWhlQAppwbeKrwGV9rZtNNQ1YsulraxSwOsKOr6WqsIatdHUcsY-HOfu9usBW48jGe3dLoUB0i8XIbh_lTFs3SbeuapRsrKWBrydB6T4Y495ckPIHvuevhn32WlTW25qRaA4gj7FnBN2948I7g6xuP9ioZ7Xf7t76JhzIODNDED20He0XB_yA2eNrpQ2xJVHjvaMP-91SN-dbmSjnL64dpcNFythvjlBvJzNAiUW2g2627hPI0XxiN0_kKm1kQ</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>PEARSON, M. S.</creator><creator>McMANUS, D. P.</creator><creator>SMYTH, D. J.</creator><creator>JONES, M. K.</creator><creator>SYKES, A. M.</creator><creator>LOUKAS, A.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20071201</creationdate><title>Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni</title><author>PEARSON, M. S. ; McMANUS, D. P. ; SMYTH, D. J. ; JONES, M. K. ; SYKES, A. M. ; LOUKAS, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-eff668d5a658a3139acd50bae56c9d76a44eed0392955a042af0b352e4e6868a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Helminth Proteins - chemistry</topic><topic>Helminth Proteins - genetics</topic><topic>Helminth Proteins - immunology</topic><topic>Helminth Proteins - metabolism</topic><topic>Invertebrates</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Protein Structure, Tertiary</topic><topic>Receptors, Cell Surface - chemistry</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - immunology</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Schistosoma mansoni</topic><topic>Schistosoma mansoni - genetics</topic><topic>Schistosoma mansoni - metabolism</topic><topic>seven transmembrane receptor</topic><topic>tegument</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PEARSON, M. S.</creatorcontrib><creatorcontrib>McMANUS, D. P.</creatorcontrib><creatorcontrib>SMYTH, D. J.</creatorcontrib><creatorcontrib>JONES, M. K.</creatorcontrib><creatorcontrib>SYKES, A. 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M.</au><au>LOUKAS, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>134</volume><issue>14</issue><spage>2001</spage><epage>2008</epage><pages>2001-2008</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>A partial cDNA sequence was obtained from the human blood fluke, Schistosoma mansoni using a signal sequence trap approach. The full-length cDNA was cloned and termed Sm-7TM. The corresponding open reading frame had 7 membrane spanning domains and shared identity with a small, novel group of seven transmembrane (7TM) receptors from vertebrates and invertebrates, including the human ee3 receptor – a heptahelical protein implicated in neuronal signalling. Phylogenetic analysis of this novel family showed that the Sm-7TM ORF formed a clade with exclusively invertebrate sequences. Based on topology modelling with ee3, Sm-7TM was predicted to possess an intracellular C-terminal tail, which was expressed as a soluble thioredoxin fusion protein (Sm-7TMC) in Escherichia coli and purified using metal ion-affinity chromatography. A polyclonal antiserum against this domain was used to detect Sm-7TM in detergent-soluble parasite extracts and to immunolocalize the receptor to the tegument of adult S. mansoni.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>17714602</pmid><doi>10.1017/S0031182007003393</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Animals Biological and medical sciences Cloning, Molecular DNA, Complementary Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Helminth Proteins - chemistry Helminth Proteins - genetics Helminth Proteins - immunology Helminth Proteins - metabolism Invertebrates Molecular Sequence Data Phylogeny Protein Structure, Tertiary Receptors, Cell Surface - chemistry Receptors, Cell Surface - genetics Receptors, Cell Surface - immunology Receptors, Cell Surface - metabolism Schistosoma mansoni Schistosoma mansoni - genetics Schistosoma mansoni - metabolism seven transmembrane receptor tegument |
| title | Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni |
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