Salivary microRNA: Discovery, Characterization, and Clinical Utility for Oral Cancer Detection
Purpose: We have previously shown that a transcriptome is found in saliva and subpanels of these mRNAs can be used as oral cancer biomarkers. In this study, we measured the presence of microRNAs (miRNA) in saliva and determined their potential as an additional set of oral cancer biomarkers. Experime...
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| Veröffentlicht in: | Clinical cancer research 2009-09, Vol.15 (17), p.5473-5477 |
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| creator | Park, Noh Jin Zhou, Hui Elashoff, David Henson, Bradley S Kastratovic, Dragana A Abemayor, Elliot Wong, David T |
| description | Purpose: We have previously shown that a transcriptome is found in saliva and subpanels of these mRNAs can be used as oral cancer
biomarkers. In this study, we measured the presence of microRNAs (miRNA) in saliva and determined their potential as an additional
set of oral cancer biomarkers.
Experimental Design: A total of 314 miRNAs were measured using reverse transcriptase-preamplification-quantitative PCR in 12 healthy controls.
Degradation pattern of endogenous and exogenous saliva miRNAs were measured at room temperature over time. Selected miRNAs
were validated in saliva of 50 oral squamous cell carcinoma patients and 50 healthy matched control subjects.
Results: We detected â¼50 miRNAs in both the whole and supernatant saliva. Endogenous saliva miRNA degraded much slower compared with
exogenous miRNA. Two miRNAs, miR-125a and miR-200a, were present in significantly lower levels ( P < 0.05) in the saliva of oral squamous cell carcinoma patients than in control subjects.
Conclusions: Both whole and supernatant saliva of healthy controls contained dozens of miRNAs, and similar to saliva mRNAs, these miRNAs
are stable. Saliva miRNAs can be used for oral cancer detection. (Clin Cancer Res 2009;15(17):5473â7) |
| doi_str_mv | 10.1158/1078-0432.CCR-09-0736 |
| format | Article |
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biomarkers. In this study, we measured the presence of microRNAs (miRNA) in saliva and determined their potential as an additional
set of oral cancer biomarkers.
Experimental Design: A total of 314 miRNAs were measured using reverse transcriptase-preamplification-quantitative PCR in 12 healthy controls.
Degradation pattern of endogenous and exogenous saliva miRNAs were measured at room temperature over time. Selected miRNAs
were validated in saliva of 50 oral squamous cell carcinoma patients and 50 healthy matched control subjects.
Results: We detected â¼50 miRNAs in both the whole and supernatant saliva. Endogenous saliva miRNA degraded much slower compared with
exogenous miRNA. Two miRNAs, miR-125a and miR-200a, were present in significantly lower levels ( P < 0.05) in the saliva of oral squamous cell carcinoma patients than in control subjects.
Conclusions: Both whole and supernatant saliva of healthy controls contained dozens of miRNAs, and similar to saliva mRNAs, these miRNAs
are stable. Saliva miRNAs can be used for oral cancer detection. (Clin Cancer Res 2009;15(17):5473â7)</description><identifier>ISSN: 1078-0432</identifier><identifier>EISSN: 1557-3265</identifier><identifier>DOI: 10.1158/1078-0432.CCR-09-0736</identifier><identifier>PMID: 19706812</identifier><identifier>CODEN: CCREF4</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Antineoplastic agents ; Biological and medical sciences ; Biomarkers, Tumor - analysis ; Biomarkers, Tumor - metabolism ; Carcinoma, Squamous Cell - diagnosis ; Carcinoma, Squamous Cell - metabolism ; Female ; Humans ; Male ; Medical sciences ; microRNA ; MicroRNAs - analysis ; MicroRNAs - metabolism ; Middle Aged ; Mouth - metabolism ; Mouth Neoplasms - diagnosis ; Mouth Neoplasms - metabolism ; oral cancer diagnosis ; Otorhinolaryngology. Stomatology ; Pharmacology. Drug treatments ; saliva ; Saliva - chemistry ; Saliva - metabolism ; Tumors ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><ispartof>Clinical cancer research, 2009-09, Vol.15 (17), p.5473-5477</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c644t-fb495d2999986c266bea839fc91c485f9ca4c409114b6c7bc4905670b9abc79d3</citedby><cites>FETCH-LOGICAL-c644t-fb495d2999986c266bea839fc91c485f9ca4c409114b6c7bc4905670b9abc79d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,3343,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22140147$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19706812$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Noh Jin</creatorcontrib><creatorcontrib>Zhou, Hui</creatorcontrib><creatorcontrib>Elashoff, David</creatorcontrib><creatorcontrib>Henson, Bradley S</creatorcontrib><creatorcontrib>Kastratovic, Dragana A</creatorcontrib><creatorcontrib>Abemayor, Elliot</creatorcontrib><creatorcontrib>Wong, David T</creatorcontrib><title>Salivary microRNA: Discovery, Characterization, and Clinical Utility for Oral Cancer Detection</title><title>Clinical cancer research</title><addtitle>Clin Cancer Res</addtitle><description>Purpose: We have previously shown that a transcriptome is found in saliva and subpanels of these mRNAs can be used as oral cancer
biomarkers. In this study, we measured the presence of microRNAs (miRNA) in saliva and determined their potential as an additional
set of oral cancer biomarkers.
Experimental Design: A total of 314 miRNAs were measured using reverse transcriptase-preamplification-quantitative PCR in 12 healthy controls.
Degradation pattern of endogenous and exogenous saliva miRNAs were measured at room temperature over time. Selected miRNAs
were validated in saliva of 50 oral squamous cell carcinoma patients and 50 healthy matched control subjects.
Results: We detected â¼50 miRNAs in both the whole and supernatant saliva. Endogenous saliva miRNA degraded much slower compared with
exogenous miRNA. Two miRNAs, miR-125a and miR-200a, were present in significantly lower levels ( P < 0.05) in the saliva of oral squamous cell carcinoma patients than in control subjects.
Conclusions: Both whole and supernatant saliva of healthy controls contained dozens of miRNAs, and similar to saliva mRNAs, these miRNAs
are stable. Saliva miRNAs can be used for oral cancer detection. (Clin Cancer Res 2009;15(17):5473â7)</description><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Carcinoma, Squamous Cell - diagnosis</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>microRNA</subject><subject>MicroRNAs - analysis</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>Mouth - metabolism</subject><subject>Mouth Neoplasms - diagnosis</subject><subject>Mouth Neoplasms - metabolism</subject><subject>oral cancer diagnosis</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Pharmacology. Drug treatments</subject><subject>saliva</subject><subject>Saliva - chemistry</subject><subject>Saliva - metabolism</subject><subject>Tumors</subject><subject>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><issn>1078-0432</issn><issn>1557-3265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkV1PHCEUhomx8bM_oQ03pjFxLN8MvWhixraamJpovZUwZxmHZnbGwuw2219fprtq5QYCD4fD8yL0jpJTSmX5kRJdFkRwdlpVNwUxBdFcbaE9KqUuOFNyO6-fmF20n9JPQqigROygXWo0USVle-j-1nVh6eIKzwPE4eb72Sd8HhIMSx9XJ7hqXXQw-hj-uDEM_Ql2_QxXXegDuA7fjaEL4wo3Q8TXMW9Urgcf8bkfPUz8IXrTuC75t5v5AN19_fKjuiiurr9dVmdXBSghxqKphZEzZvIoFTClau9KbhowFEQpGwNOgCCGUlEr0DUIQ6TSpDauBm1m_AB9Xtd9XNRzPwPfj7kd-xjDPP_NDi7Y1yd9aO3DsLRMS8alzAU-bArE4dfCp9HOswXfda73wyJZzQXhUqgyk3JNZl0pRd88v0KJnaKxk3Y7abc5GkuMnaLJ997_3-LLrU0WGTjaAC5luU3MLkN65hijIgeoM3e85trw0P4O0Vv4Zz365F2E1lJpqbYyo_wv0DSmXw</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Park, Noh Jin</creator><creator>Zhou, Hui</creator><creator>Elashoff, David</creator><creator>Henson, Bradley S</creator><creator>Kastratovic, Dragana A</creator><creator>Abemayor, Elliot</creator><creator>Wong, David T</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090901</creationdate><title>Salivary microRNA: Discovery, Characterization, and Clinical Utility for Oral Cancer Detection</title><author>Park, Noh Jin ; Zhou, Hui ; Elashoff, David ; Henson, Bradley S ; Kastratovic, Dragana A ; Abemayor, Elliot ; Wong, David T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c644t-fb495d2999986c266bea839fc91c485f9ca4c409114b6c7bc4905670b9abc79d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Carcinoma, Squamous Cell - diagnosis</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>microRNA</topic><topic>MicroRNAs - analysis</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>Mouth - metabolism</topic><topic>Mouth Neoplasms - diagnosis</topic><topic>Mouth Neoplasms - metabolism</topic><topic>oral cancer diagnosis</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Pharmacology. Drug treatments</topic><topic>saliva</topic><topic>Saliva - chemistry</topic><topic>Saliva - metabolism</topic><topic>Tumors</topic><topic>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Noh Jin</creatorcontrib><creatorcontrib>Zhou, Hui</creatorcontrib><creatorcontrib>Elashoff, David</creatorcontrib><creatorcontrib>Henson, Bradley S</creatorcontrib><creatorcontrib>Kastratovic, Dragana A</creatorcontrib><creatorcontrib>Abemayor, Elliot</creatorcontrib><creatorcontrib>Wong, David T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Noh Jin</au><au>Zhou, Hui</au><au>Elashoff, David</au><au>Henson, Bradley S</au><au>Kastratovic, Dragana A</au><au>Abemayor, Elliot</au><au>Wong, David T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Salivary microRNA: Discovery, Characterization, and Clinical Utility for Oral Cancer Detection</atitle><jtitle>Clinical cancer research</jtitle><addtitle>Clin Cancer Res</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>15</volume><issue>17</issue><spage>5473</spage><epage>5477</epage><pages>5473-5477</pages><issn>1078-0432</issn><eissn>1557-3265</eissn><coden>CCREF4</coden><abstract>Purpose: We have previously shown that a transcriptome is found in saliva and subpanels of these mRNAs can be used as oral cancer
biomarkers. In this study, we measured the presence of microRNAs (miRNA) in saliva and determined their potential as an additional
set of oral cancer biomarkers.
Experimental Design: A total of 314 miRNAs were measured using reverse transcriptase-preamplification-quantitative PCR in 12 healthy controls.
Degradation pattern of endogenous and exogenous saliva miRNAs were measured at room temperature over time. Selected miRNAs
were validated in saliva of 50 oral squamous cell carcinoma patients and 50 healthy matched control subjects.
Results: We detected â¼50 miRNAs in both the whole and supernatant saliva. Endogenous saliva miRNA degraded much slower compared with
exogenous miRNA. Two miRNAs, miR-125a and miR-200a, were present in significantly lower levels ( P < 0.05) in the saliva of oral squamous cell carcinoma patients than in control subjects.
Conclusions: Both whole and supernatant saliva of healthy controls contained dozens of miRNAs, and similar to saliva mRNAs, these miRNAs
are stable. Saliva miRNAs can be used for oral cancer detection. (Clin Cancer Res 2009;15(17):5473â7)</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>19706812</pmid><doi>10.1158/1078-0432.CCR-09-0736</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Antineoplastic agents Biological and medical sciences Biomarkers, Tumor - analysis Biomarkers, Tumor - metabolism Carcinoma, Squamous Cell - diagnosis Carcinoma, Squamous Cell - metabolism Female Humans Male Medical sciences microRNA MicroRNAs - analysis MicroRNAs - metabolism Middle Aged Mouth - metabolism Mouth Neoplasms - diagnosis Mouth Neoplasms - metabolism oral cancer diagnosis Otorhinolaryngology. Stomatology Pharmacology. Drug treatments saliva Saliva - chemistry Saliva - metabolism Tumors Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| title | Salivary microRNA: Discovery, Characterization, and Clinical Utility for Oral Cancer Detection |
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