Olfactory perireceptor and receptor events in moths: a kinetic model revised
Modelling reveals that within about 3 ms after entering the sensillum lymph, 17% of total pheromone is enzymatically degraded while 83% is bound to the pheromone-binding protein (PBP) and thereby largely protected from enzymatic degradation. The latter proceeds within minutes, 20,000-fold more slowl...
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description | Modelling reveals that within about 3 ms after entering the sensillum lymph, 17% of total pheromone is enzymatically degraded while 83% is bound to the pheromone-binding protein (PBP) and thereby largely protected from enzymatic degradation. The latter proceeds within minutes, 20,000-fold more slowly than with the free pheromone. In vivo the complex pheromone–PBP interacts with the receptor molecule. At weak stimulation the half-life of the active complex is 0.8 s due to the postulated pheromone deactivation. Most likely this process is enzymatically catalysed; it changes the PBP into a scavenger form, possibly by interference with the C-terminus. The indirectly determined PBP concentration (3.8 mM) is close to direct measurements. The calculated density of receptor molecules within the plasma membrane of the receptor neuron reaches up to 6,000 units per μm
2
. This is compared with the estimated densities of the sensory-neuron membrane protein and of ion channels. The EC
50
of the model pheromone–PBP complex interacting with the receptor molecules is 6.8 μM, as compared with the EC
50
= 1.5 μM of bombykol recently determined using heterologous expression. A possible mechanism widening the range of stimulus intensities covered by the dose–response curve of the receptor-potential is proposed. |
doi_str_mv | 10.1007/s00359-009-0461-4 |
format | Article |
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2
. This is compared with the estimated densities of the sensory-neuron membrane protein and of ion channels. The EC
50
of the model pheromone–PBP complex interacting with the receptor molecules is 6.8 μM, as compared with the EC
50
= 1.5 μM of bombykol recently determined using heterologous expression. A possible mechanism widening the range of stimulus intensities covered by the dose–response curve of the receptor-potential is proposed.</description><identifier>ISSN: 0340-7594</identifier><identifier>EISSN: 1432-1351</identifier><identifier>DOI: 10.1007/s00359-009-0461-4</identifier><identifier>PMID: 19697043</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animal Physiology ; Animals ; Biological Transport ; Biomedical and Life Sciences ; C-Terminus ; Carrier Proteins - metabolism ; Computer Simulation ; Deactivation ; Fatty Alcohols - metabolism ; Insect Proteins - metabolism ; Ion channels ; Kinetics ; Life Sciences ; Lymph ; Membrane proteins ; Models, Biological ; Molecular modelling ; Moths - physiology ; Neurons ; Neurosciences ; Pheromone-binding protein ; Pheromones ; Pheromones - metabolism ; Plasma membranes ; Protein Conformation ; Receptor density ; Receptors, Odorant - metabolism ; Review ; Signal Transduction ; Smell ; Structure-Activity Relationship ; Zoology</subject><ispartof>Journal of Comparative Physiology, 2009-10, Vol.195 (10), p.895-922</ispartof><rights>The Author(s) 2009</rights><rights>Springer-Verlag 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-2f74774bbcb1c9553d1a78dac1f0b8b84e04fa9d28b723992262a429ad9ac4f63</citedby><cites>FETCH-LOGICAL-c565t-2f74774bbcb1c9553d1a78dac1f0b8b84e04fa9d28b723992262a429ad9ac4f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00359-009-0461-4$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00359-009-0461-4$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19697043$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaissling, Karl-Ernst</creatorcontrib><title>Olfactory perireceptor and receptor events in moths: a kinetic model revised</title><title>Journal of Comparative Physiology</title><addtitle>J Comp Physiol A</addtitle><addtitle>J Comp Physiol A Neuroethol Sens Neural Behav Physiol</addtitle><description>Modelling reveals that within about 3 ms after entering the sensillum lymph, 17% of total pheromone is enzymatically degraded while 83% is bound to the pheromone-binding protein (PBP) and thereby largely protected from enzymatic degradation. The latter proceeds within minutes, 20,000-fold more slowly than with the free pheromone. In vivo the complex pheromone–PBP interacts with the receptor molecule. At weak stimulation the half-life of the active complex is 0.8 s due to the postulated pheromone deactivation. Most likely this process is enzymatically catalysed; it changes the PBP into a scavenger form, possibly by interference with the C-terminus. The indirectly determined PBP concentration (3.8 mM) is close to direct measurements. The calculated density of receptor molecules within the plasma membrane of the receptor neuron reaches up to 6,000 units per μm
2
. This is compared with the estimated densities of the sensory-neuron membrane protein and of ion channels. The EC
50
of the model pheromone–PBP complex interacting with the receptor molecules is 6.8 μM, as compared with the EC
50
= 1.5 μM of bombykol recently determined using heterologous expression. A possible mechanism widening the range of stimulus intensities covered by the dose–response curve of the receptor-potential is proposed.</description><subject>Animal Physiology</subject><subject>Animals</subject><subject>Biological Transport</subject><subject>Biomedical and Life Sciences</subject><subject>C-Terminus</subject><subject>Carrier Proteins - metabolism</subject><subject>Computer Simulation</subject><subject>Deactivation</subject><subject>Fatty Alcohols - metabolism</subject><subject>Insect Proteins - metabolism</subject><subject>Ion channels</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Lymph</subject><subject>Membrane proteins</subject><subject>Models, Biological</subject><subject>Molecular modelling</subject><subject>Moths - physiology</subject><subject>Neurons</subject><subject>Neurosciences</subject><subject>Pheromone-binding protein</subject><subject>Pheromones</subject><subject>Pheromones - metabolism</subject><subject>Plasma membranes</subject><subject>Protein Conformation</subject><subject>Receptor density</subject><subject>Receptors, Odorant - metabolism</subject><subject>Review</subject><subject>Signal Transduction</subject><subject>Smell</subject><subject>Structure-Activity Relationship</subject><subject>Zoology</subject><issn>0340-7594</issn><issn>1432-1351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kcFOGzEQhq2qqAmBB-CCVj20py1j79hec0BCqLSVInGBs-X1esFhsxvs3Ui8fR0lSgoSPVij8Xzze8Y_IWcUflAAeREBCq5ygHRQ0Bw_kSnFguW04PQzmUKBkEuucEKOY1wAAKOMfiETqoSSgMWUzO_axtihD6_ZygUfnHWrlGWmq7N94tauG2Lmu2zZD0_xMjPZs-_c4G26qF2byLWPrj4hR41pozvdxRl5uP15f_M7n9_9-nNzPc8tF3zIWSNRSqwqW1GrOC9qamRZG0sbqMqqRAfYGFWzspKsUIoxwQwyZWplLDaimJGrre5qrJautmm6YFq9Cn5pwqvujddvK51_0o_9WjOJipYsCXzfCYT-ZXRx0EsfrWtb07l-jLoUUnKQjCfy239JIYVATD7MyNd34KIfQ5e-QTNA5CVTmCC6hWzoYwyu2c9MQW8s1VtLdbJUbyzVm57zf5c9dOw8TADbAjGVukcXDi9_rPoXgoissg</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Kaissling, Karl-Ernst</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20091001</creationdate><title>Olfactory perireceptor and receptor events in moths: a kinetic model revised</title><author>Kaissling, Karl-Ernst</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-2f74774bbcb1c9553d1a78dac1f0b8b84e04fa9d28b723992262a429ad9ac4f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animal Physiology</topic><topic>Animals</topic><topic>Biological Transport</topic><topic>Biomedical and Life Sciences</topic><topic>C-Terminus</topic><topic>Carrier Proteins - metabolism</topic><topic>Computer Simulation</topic><topic>Deactivation</topic><topic>Fatty Alcohols - metabolism</topic><topic>Insect Proteins - metabolism</topic><topic>Ion channels</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Lymph</topic><topic>Membrane proteins</topic><topic>Models, Biological</topic><topic>Molecular modelling</topic><topic>Moths - physiology</topic><topic>Neurons</topic><topic>Neurosciences</topic><topic>Pheromone-binding protein</topic><topic>Pheromones</topic><topic>Pheromones - metabolism</topic><topic>Plasma membranes</topic><topic>Protein Conformation</topic><topic>Receptor density</topic><topic>Receptors, Odorant - metabolism</topic><topic>Review</topic><topic>Signal Transduction</topic><topic>Smell</topic><topic>Structure-Activity Relationship</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaissling, Karl-Ernst</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Comparative Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaissling, Karl-Ernst</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Olfactory perireceptor and receptor events in moths: a kinetic model revised</atitle><jtitle>Journal of Comparative Physiology</jtitle><stitle>J Comp Physiol A</stitle><addtitle>J Comp Physiol A Neuroethol Sens Neural Behav Physiol</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>195</volume><issue>10</issue><spage>895</spage><epage>922</epage><pages>895-922</pages><issn>0340-7594</issn><eissn>1432-1351</eissn><abstract>Modelling reveals that within about 3 ms after entering the sensillum lymph, 17% of total pheromone is enzymatically degraded while 83% is bound to the pheromone-binding protein (PBP) and thereby largely protected from enzymatic degradation. The latter proceeds within minutes, 20,000-fold more slowly than with the free pheromone. In vivo the complex pheromone–PBP interacts with the receptor molecule. At weak stimulation the half-life of the active complex is 0.8 s due to the postulated pheromone deactivation. Most likely this process is enzymatically catalysed; it changes the PBP into a scavenger form, possibly by interference with the C-terminus. The indirectly determined PBP concentration (3.8 mM) is close to direct measurements. The calculated density of receptor molecules within the plasma membrane of the receptor neuron reaches up to 6,000 units per μm
2
. This is compared with the estimated densities of the sensory-neuron membrane protein and of ion channels. The EC
50
of the model pheromone–PBP complex interacting with the receptor molecules is 6.8 μM, as compared with the EC
50
= 1.5 μM of bombykol recently determined using heterologous expression. A possible mechanism widening the range of stimulus intensities covered by the dose–response curve of the receptor-potential is proposed.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>19697043</pmid><doi>10.1007/s00359-009-0461-4</doi><tpages>28</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animal Physiology Animals Biological Transport Biomedical and Life Sciences C-Terminus Carrier Proteins - metabolism Computer Simulation Deactivation Fatty Alcohols - metabolism Insect Proteins - metabolism Ion channels Kinetics Life Sciences Lymph Membrane proteins Models, Biological Molecular modelling Moths - physiology Neurons Neurosciences Pheromone-binding protein Pheromones Pheromones - metabolism Plasma membranes Protein Conformation Receptor density Receptors, Odorant - metabolism Review Signal Transduction Smell Structure-Activity Relationship Zoology |
title | Olfactory perireceptor and receptor events in moths: a kinetic model revised |
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