Limited transcriptional response of ovine microglia to prion accumulation
The conversion of normal cellular prion protein to disease-associated prion protein (PrP Sc) is a fundamental component of prion disease pathogenesis. The molecular mechanisms contributing to prion conversion and the impact of PrP Sc accumulation on cellular biology are not fully understood. To furt...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2009-08, Vol.386 (2), p.345-350 |
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| creator | Stanton, James B. Knowles, Donald P. Call, Douglas R. Mathison, Bruce A. Baszler, Timothy V. |
| description | The conversion of normal cellular prion protein to disease-associated prion protein (PrP
Sc) is a fundamental component of prion disease pathogenesis. The molecular mechanisms contributing to prion conversion and the impact of PrP
Sc accumulation on cellular biology are not fully understood. To further define the molecular changes associated with PrP
Sc accumulation in cultured cells, the transcriptional profile of PrP
Sc-accumulating primary ovine microglia was compared to the profile of PrP
Sc-lacking microglia using the Affymetrix Bovine Genome Array. The experimental design included three biological replicates, each with three technical replicates, and samples that were collected at the point of near maximal PrP
Sc accumulation levels as measured by ELISA. The array analysis revealed only 19 upregulated genes and 30 downregulated genes in PrP
Sc-accumulating microglia. The results support the hypothesis that chronic PrP
Sc accumulation in cultured microglia results in a limited transcriptional response. |
| doi_str_mv | 10.1016/j.bbrc.2009.06.030 |
| format | Article |
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Sc) is a fundamental component of prion disease pathogenesis. The molecular mechanisms contributing to prion conversion and the impact of PrP
Sc accumulation on cellular biology are not fully understood. To further define the molecular changes associated with PrP
Sc accumulation in cultured cells, the transcriptional profile of PrP
Sc-accumulating primary ovine microglia was compared to the profile of PrP
Sc-lacking microglia using the Affymetrix Bovine Genome Array. The experimental design included three biological replicates, each with three technical replicates, and samples that were collected at the point of near maximal PrP
Sc accumulation levels as measured by ELISA. The array analysis revealed only 19 upregulated genes and 30 downregulated genes in PrP
Sc-accumulating microglia. The results support the hypothesis that chronic PrP
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Sc) is a fundamental component of prion disease pathogenesis. The molecular mechanisms contributing to prion conversion and the impact of PrP
Sc accumulation on cellular biology are not fully understood. To further define the molecular changes associated with PrP
Sc accumulation in cultured cells, the transcriptional profile of PrP
Sc-accumulating primary ovine microglia was compared to the profile of PrP
Sc-lacking microglia using the Affymetrix Bovine Genome Array. The experimental design included three biological replicates, each with three technical replicates, and samples that were collected at the point of near maximal PrP
Sc accumulation levels as measured by ELISA. The array analysis revealed only 19 upregulated genes and 30 downregulated genes in PrP
Sc-accumulating microglia. The results support the hypothesis that chronic PrP
Sc accumulation in cultured microglia results in a limited transcriptional response.</description><subject>Animals</subject><subject>Cattle</subject><subject>enzyme-linked immunosorbent assay</subject><subject>gene expression</subject><subject>Gene Expression Profiling</subject><subject>gene expression regulation</subject><subject>Microarray</subject><subject>Microglia</subject><subject>Microglia - metabolism</subject><subject>neuroglia</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Ovine</subject><subject>Prion</subject><subject>prion diseases</subject><subject>prions</subject><subject>PrPSc Proteins - metabolism</subject><subject>Scrapie</subject><subject>sheep</subject><subject>Sheep - genetics</subject><subject>Sheep - metabolism</subject><subject>transcription (genetics)</subject><subject>Transcription, Genetic</subject><issn>0006-291X</issn><issn>1090-2104</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUur1DAYhoMonvHoH3ChXblr_b7cOgER5ODlwIALPeAupGkyZmibMWkH_PemzOBl41llked9eJOXkOcIDQLK14em65JtKIBqQDbA4AHZICioKQJ_SDYAIGuq8NsVeZLzAQCRS_WYXKESlHHBNuR2F8Ywu76ak5myTeE4hziZoUouH-OUXRV9FU9hctUYbIr7IZhqjtUxFawy1i7jMpg185Q88mbI7tnlvCZ3H95_vflU7z5_vL15t6utYGquvWWWonQluhVADQpUrXTccgHeMN6h94Ia8LRXbGtEp4AJ9Aa3nILoO3ZN3p69x6UbXW_dVKoPuhQaTfqpown635spfNf7eNK05VJKLIJXF0GKPxaXZz2GbN0wmMnFJWvZcoFSsntBLpliXLX3gmWhVgpYjfQMlp_MOTn_uzaCXjfVB71uuiaUBqnLpiX04u8H_4lcRizAyzPgTdRmn0LWd18oICvCtgW-Kt6cCVeGOQWXdLbBTdb1ITk76z6G_zX4BQCivVA</recordid><startdate>20090821</startdate><enddate>20090821</enddate><creator>Stanton, James B.</creator><creator>Knowles, Donald P.</creator><creator>Call, Douglas R.</creator><creator>Mathison, Bruce A.</creator><creator>Baszler, Timothy V.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090821</creationdate><title>Limited transcriptional response of ovine microglia to prion accumulation</title><author>Stanton, James B. ; 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Sc) is a fundamental component of prion disease pathogenesis. The molecular mechanisms contributing to prion conversion and the impact of PrP
Sc accumulation on cellular biology are not fully understood. To further define the molecular changes associated with PrP
Sc accumulation in cultured cells, the transcriptional profile of PrP
Sc-accumulating primary ovine microglia was compared to the profile of PrP
Sc-lacking microglia using the Affymetrix Bovine Genome Array. The experimental design included three biological replicates, each with three technical replicates, and samples that were collected at the point of near maximal PrP
Sc accumulation levels as measured by ELISA. The array analysis revealed only 19 upregulated genes and 30 downregulated genes in PrP
Sc-accumulating microglia. The results support the hypothesis that chronic PrP
Sc accumulation in cultured microglia results in a limited transcriptional response.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19523453</pmid><doi>10.1016/j.bbrc.2009.06.030</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Cattle enzyme-linked immunosorbent assay gene expression Gene Expression Profiling gene expression regulation Microarray Microglia Microglia - metabolism neuroglia Oligonucleotide Array Sequence Analysis Ovine Prion prion diseases prions PrPSc Proteins - metabolism Scrapie sheep Sheep - genetics Sheep - metabolism transcription (genetics) Transcription, Genetic |
| title | Limited transcriptional response of ovine microglia to prion accumulation |
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