TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP
Transient receptor potential V3 (TRPV3) and TRPV4 are heat-activated cation channels expressed in keratinocytes. It has been proposed that heat-activation of TRPV3 and/or TRPV4 in the skin may release diffusible molecules which would then activate termini of neighboring dorsal root ganglion (DRG) ne...
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| Veröffentlicht in: | Pflügers Archiv 2009-10, Vol.458 (6), p.1093-1102 |
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| creator | Mandadi, Sravan Sokabe, Takaaki Shibasaki, Koji Katanosaka, Kimiaki Mizuno, Atsuko Moqrich, Aziz Patapoutian, Ardem Fukumi-Tominaga, Tomoko Mizumura, Kazue Tominaga, Makoto |
| description | Transient receptor potential V3 (TRPV3) and TRPV4 are heat-activated cation channels expressed in keratinocytes. It has been proposed that heat-activation of TRPV3 and/or TRPV4 in the skin may release diffusible molecules which would then activate termini of neighboring dorsal root ganglion (DRG) neurons. Here we show that adenosine triphosphate (ATP) is such a candidate molecule released from keratinocytes upon heating in the co-culture systems. Using TRPV1-deficient DRG neurons, we found that increase in cytosolic Ca
2+
-concentration in DRG neurons upon heating was observed only when neurons were co-cultured with keratinocytes, and this increase was blocked by P2 purinoreceptor antagonists, PPADS and suramin. In a co-culture of keratinocytes with HEK293 cells (transfected with P2X
2
cDNA to serve as a bio-sensor), we observed that heat-activated keratinocytes secretes ATP, and that ATP release is compromised in keratinocytes from TRPV3-deficient mice. This study provides evidence that ATP is a messenger molecule for mainly TRPV3-mediated thermotransduction in skin. |
| doi_str_mv | 10.1007/s00424-009-0703-x |
| format | Article |
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2+
-concentration in DRG neurons upon heating was observed only when neurons were co-cultured with keratinocytes, and this increase was blocked by P2 purinoreceptor antagonists, PPADS and suramin. In a co-culture of keratinocytes with HEK293 cells (transfected with P2X
2
cDNA to serve as a bio-sensor), we observed that heat-activated keratinocytes secretes ATP, and that ATP release is compromised in keratinocytes from TRPV3-deficient mice. This study provides evidence that ATP is a messenger molecule for mainly TRPV3-mediated thermotransduction in skin.</description><identifier>ISSN: 0031-6768</identifier><identifier>EISSN: 1432-2013</identifier><identifier>DOI: 10.1007/s00424-009-0703-x</identifier><identifier>PMID: 19669158</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Adenosine Triphosphate - physiology ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Calcium - metabolism ; Cell Biology ; Cells, Cultured ; Coculture Techniques ; Ganglia, Spinal - cytology ; Glutamic Acid - metabolism ; Hot Temperature ; Human Physiology ; Humans ; Ion Channels ; Ion Channels, Receptors and Transporters ; Keratinocytes - physiology ; Male ; Mice ; Mice, Inbred C57BL ; Molecular Medicine ; Neurons ; Neurosciences ; Patch-Clamp Techniques ; Receptors ; Receptors and Transporters ; Sensory Receptor Cells - metabolism ; Serotonin - metabolism ; Signal Transduction - physiology ; Skin - metabolism ; TRPV Cation Channels - physiology</subject><ispartof>Pflügers Archiv, 2009-10, Vol.458 (6), p.1093-1102</ispartof><rights>The Author(s) 2009</rights><rights>Springer-Verlag 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p327t-38a2079996d6976009e4f20c2c409ff42476f25bcbc1bd97e1696b4fe2ed1363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00424-009-0703-x$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00424-009-0703-x$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19669158$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mandadi, Sravan</creatorcontrib><creatorcontrib>Sokabe, Takaaki</creatorcontrib><creatorcontrib>Shibasaki, Koji</creatorcontrib><creatorcontrib>Katanosaka, Kimiaki</creatorcontrib><creatorcontrib>Mizuno, Atsuko</creatorcontrib><creatorcontrib>Moqrich, Aziz</creatorcontrib><creatorcontrib>Patapoutian, Ardem</creatorcontrib><creatorcontrib>Fukumi-Tominaga, Tomoko</creatorcontrib><creatorcontrib>Mizumura, Kazue</creatorcontrib><creatorcontrib>Tominaga, Makoto</creatorcontrib><title>TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP</title><title>Pflügers Archiv</title><addtitle>Pflugers Arch - Eur J Physiol</addtitle><addtitle>Pflugers Arch</addtitle><description>Transient receptor potential V3 (TRPV3) and TRPV4 are heat-activated cation channels expressed in keratinocytes. It has been proposed that heat-activation of TRPV3 and/or TRPV4 in the skin may release diffusible molecules which would then activate termini of neighboring dorsal root ganglion (DRG) neurons. Here we show that adenosine triphosphate (ATP) is such a candidate molecule released from keratinocytes upon heating in the co-culture systems. Using TRPV1-deficient DRG neurons, we found that increase in cytosolic Ca
2+
-concentration in DRG neurons upon heating was observed only when neurons were co-cultured with keratinocytes, and this increase was blocked by P2 purinoreceptor antagonists, PPADS and suramin. In a co-culture of keratinocytes with HEK293 cells (transfected with P2X
2
cDNA to serve as a bio-sensor), we observed that heat-activated keratinocytes secretes ATP, and that ATP release is compromised in keratinocytes from TRPV3-deficient mice. This study provides evidence that ATP is a messenger molecule for mainly TRPV3-mediated thermotransduction in skin.</description><subject>Adenosine Triphosphate - physiology</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Calcium - metabolism</subject><subject>Cell Biology</subject><subject>Cells, Cultured</subject><subject>Coculture Techniques</subject><subject>Ganglia, Spinal - cytology</subject><subject>Glutamic Acid - metabolism</subject><subject>Hot Temperature</subject><subject>Human Physiology</subject><subject>Humans</subject><subject>Ion Channels</subject><subject>Ion Channels, Receptors and Transporters</subject><subject>Keratinocytes - physiology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Medicine</subject><subject>Neurons</subject><subject>Neurosciences</subject><subject>Patch-Clamp Techniques</subject><subject>Receptors</subject><subject>Receptors and Transporters</subject><subject>Sensory Receptor Cells - metabolism</subject><subject>Serotonin - metabolism</subject><subject>Signal Transduction - physiology</subject><subject>Skin - metabolism</subject><subject>TRPV Cation Channels - physiology</subject><issn>0031-6768</issn><issn>1432-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkkFPGzEQha2KCkLoD-gFrTjQ05bx2GvHF6QIUVoJqaiNuFq7m9mwNGsHexeRf19HoUA5IHwZS-_T07yZYewzh68cQJ9EAIkyBzA5aBD5wwc24lJgjsDFDhsBCJ4rrSZ7bD_GWwBAOcFdtseNUoYXkxH7Pft1dS2y1mV_KJR963y97ilmfShd7No-_ahbbaQhUMIaH7qEeZf1Povkog_rzNEQvIvZfVtm09nVAfvYlMtInx7rmM2-nc_OvueXPy9-nE0v85VA3ediUiJoY4yaK6NVSkGyQaixlmCaJiXTqsGiqquaV3OjiSujKtkQ0pwLJcbsdGu7GqqO5jW51PTSrkLblWFtfdna_xXX3tiFv7eoZaFQJIMvjwbB3w0Ue9u1sablsnTkh2i1FIVQ6SXy-E0SuZQG0bwLTJPnCTx6Bd76Ibg0LotpTQaw2DR4-DLhU7R_60sAboGYJLeg8OzCwW5uxG5vxKbp2s2N2AfxF3IHrSA</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Mandadi, Sravan</creator><creator>Sokabe, Takaaki</creator><creator>Shibasaki, Koji</creator><creator>Katanosaka, Kimiaki</creator><creator>Mizuno, Atsuko</creator><creator>Moqrich, Aziz</creator><creator>Patapoutian, Ardem</creator><creator>Fukumi-Tominaga, Tomoko</creator><creator>Mizumura, Kazue</creator><creator>Tominaga, Makoto</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope><scope>5PM</scope></search><sort><creationdate>20091001</creationdate><title>TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP</title><author>Mandadi, Sravan ; Sokabe, Takaaki ; Shibasaki, Koji ; Katanosaka, Kimiaki ; Mizuno, Atsuko ; Moqrich, Aziz ; Patapoutian, Ardem ; Fukumi-Tominaga, Tomoko ; Mizumura, Kazue ; Tominaga, Makoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p327t-38a2079996d6976009e4f20c2c409ff42476f25bcbc1bd97e1696b4fe2ed1363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adenosine Triphosphate - 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Eur J Physiol</stitle><addtitle>Pflugers Arch</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>458</volume><issue>6</issue><spage>1093</spage><epage>1102</epage><pages>1093-1102</pages><issn>0031-6768</issn><eissn>1432-2013</eissn><abstract>Transient receptor potential V3 (TRPV3) and TRPV4 are heat-activated cation channels expressed in keratinocytes. It has been proposed that heat-activation of TRPV3 and/or TRPV4 in the skin may release diffusible molecules which would then activate termini of neighboring dorsal root ganglion (DRG) neurons. Here we show that adenosine triphosphate (ATP) is such a candidate molecule released from keratinocytes upon heating in the co-culture systems. Using TRPV1-deficient DRG neurons, we found that increase in cytosolic Ca
2+
-concentration in DRG neurons upon heating was observed only when neurons were co-cultured with keratinocytes, and this increase was blocked by P2 purinoreceptor antagonists, PPADS and suramin. In a co-culture of keratinocytes with HEK293 cells (transfected with P2X
2
cDNA to serve as a bio-sensor), we observed that heat-activated keratinocytes secretes ATP, and that ATP release is compromised in keratinocytes from TRPV3-deficient mice. This study provides evidence that ATP is a messenger molecule for mainly TRPV3-mediated thermotransduction in skin.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>19669158</pmid><doi>10.1007/s00424-009-0703-x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Pflügers Archiv, 2009-10, Vol.458 (6), p.1093-1102 |
| issn | 0031-6768 1432-2013 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Adenosine Triphosphate - physiology Animals Biomedical and Life Sciences Biomedicine Calcium - metabolism Cell Biology Cells, Cultured Coculture Techniques Ganglia, Spinal - cytology Glutamic Acid - metabolism Hot Temperature Human Physiology Humans Ion Channels Ion Channels, Receptors and Transporters Keratinocytes - physiology Male Mice Mice, Inbred C57BL Molecular Medicine Neurons Neurosciences Patch-Clamp Techniques Receptors Receptors and Transporters Sensory Receptor Cells - metabolism Serotonin - metabolism Signal Transduction - physiology Skin - metabolism TRPV Cation Channels - physiology |
| title | TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP |
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