SurR: a transcriptional activator and repressor controlling hydrogen and elemental sulphur metabolism in Pyrococcus furiosus
This work describes the identification and characterization of SurR, Pyrococcus furiosus sulphur (S⁰) response regulator. SurR was captured from cell extract using promoter DNA of a hydrogenase operon that is downregulated in the primary response of P. furiosus to S⁰, as revealed by DNA microarray e...
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| Veröffentlicht in: | Molecular microbiology 2009-01, Vol.71 (2), p.332-349 |
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| creator | Lipscomb, Gina L Keese, Annette M Cowart, Darin M Schut, Gerrit J Thomm, Michael Adams, Michael W.W Scott, Robert A |
| description | This work describes the identification and characterization of SurR, Pyrococcus furiosus sulphur (S⁰) response regulator. SurR was captured from cell extract using promoter DNA of a hydrogenase operon that is downregulated in the primary response of P. furiosus to S⁰, as revealed by DNA microarray experiments. SurR was validated as a sequence-specific DNA binding protein, and characterization of the SurR DNA binding motif GTTn₃AAC led to the identification of several target genes that contain an extended motif in their promoters. A number of these were validated to contain upstream SurR binding sites. These SurR targets strongly correspond with open reading frames and operons both up- and downregulated in the primary response to S⁰. In vitro transcription revealed that SurR is an activator for its own gene as well as for two hydrogenase operons whose expression is downregulated during the primary S⁰ response; it is also a repressor for two genes upregulated during the primary S⁰ response, one of which encodes the primary S⁰-reducing enzyme NAD(P)H sulphur reductase. Herein we give evidence for the role of SurR in both mediating the primary response to S⁰ and controlling hydrogen production in P. furiosus. |
| doi_str_mv | 10.1111/j.1365-2958.2008.06525.x |
| format | Article |
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SurR was captured from cell extract using promoter DNA of a hydrogenase operon that is downregulated in the primary response of P. furiosus to S⁰, as revealed by DNA microarray experiments. SurR was validated as a sequence-specific DNA binding protein, and characterization of the SurR DNA binding motif GTTn₃AAC led to the identification of several target genes that contain an extended motif in their promoters. A number of these were validated to contain upstream SurR binding sites. These SurR targets strongly correspond with open reading frames and operons both up- and downregulated in the primary response to S⁰. In vitro transcription revealed that SurR is an activator for its own gene as well as for two hydrogenase operons whose expression is downregulated during the primary S⁰ response; it is also a repressor for two genes upregulated during the primary S⁰ response, one of which encodes the primary S⁰-reducing enzyme NAD(P)H sulphur reductase. Herein we give evidence for the role of SurR in both mediating the primary response to S⁰ and controlling hydrogen production in P. furiosus.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.2008.06525.x</identifier><identifier>PMID: 19017274</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Archaeal Proteins - genetics ; Archaeal Proteins - metabolism ; Bacteria ; Bacteriology ; Binding sites ; Biological and medical sciences ; Cloning, Molecular ; Deoxyribonucleic acid ; DNA ; DNA Footprinting ; DNA, Archaeal - genetics ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Electrophoretic Mobility Shift Assay ; Experiments ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Archaeal ; Genes, Archaeal ; Genes, Regulator ; Hydrogen - metabolism ; Microbiology ; Open Reading Frames ; Operon ; Promoter Regions, Genetic ; Proteins ; Pyrococcus furiosus ; Pyrococcus furiosus - genetics ; Pyrococcus furiosus - metabolism ; Sulfur - metabolism ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic ; Transcriptional Activation</subject><ispartof>Molecular microbiology, 2009-01, Vol.71 (2), p.332-349</ispartof><rights>2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Ltd. 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SurR was captured from cell extract using promoter DNA of a hydrogenase operon that is downregulated in the primary response of P. furiosus to S⁰, as revealed by DNA microarray experiments. SurR was validated as a sequence-specific DNA binding protein, and characterization of the SurR DNA binding motif GTTn₃AAC led to the identification of several target genes that contain an extended motif in their promoters. A number of these were validated to contain upstream SurR binding sites. These SurR targets strongly correspond with open reading frames and operons both up- and downregulated in the primary response to S⁰. In vitro transcription revealed that SurR is an activator for its own gene as well as for two hydrogenase operons whose expression is downregulated during the primary S⁰ response; it is also a repressor for two genes upregulated during the primary S⁰ response, one of which encodes the primary S⁰-reducing enzyme NAD(P)H sulphur reductase. Herein we give evidence for the role of SurR in both mediating the primary response to S⁰ and controlling hydrogen production in P. furiosus.</description><subject>Archaeal Proteins - genetics</subject><subject>Archaeal Proteins - metabolism</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Binding sites</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Footprinting</subject><subject>DNA, Archaeal - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Electrophoretic Mobility Shift Assay</subject><subject>Experiments</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Archaeal</subject><subject>Genes, Archaeal</subject><subject>Genes, Regulator</subject><subject>Hydrogen - metabolism</subject><subject>Microbiology</subject><subject>Open Reading Frames</subject><subject>Operon</subject><subject>Promoter Regions, Genetic</subject><subject>Proteins</subject><subject>Pyrococcus furiosus</subject><subject>Pyrococcus furiosus - genetics</subject><subject>Pyrococcus furiosus - metabolism</subject><subject>Sulfur - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcriptional Activation</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkl1rFDEUhgdR7Fr9CxoEvZsxH5MvQUGKtoUWxVrwLmQzmd0smcmYzNQu-OPNdJf140Zzk4Q878k55z1FARCsUF6vNhUijJZYUlFhCEUFGcW0ur1XLA4P94sFlBSWROCvR8WjlDYQIgIZeVgcIQkRx7xeFD-upvj5NdBgjLpPJrphdKHXHmgzuhs9hgh034Boh2hTyjcT-jEG712_AuttE8PK9neI9baz_ZilafLDeoqgs6NeBu9SB1wPPm1jMMGYKYF2ii6kKT0uHrTaJ_tkvx8X1x_efzk5Ky8-np6fvLsoDRU1LenSGi6pQZQY2DCBEIWyNpxBwriEpGm5EcwSTZk0tSVC5h6xphHUYtiSmhwXb3dxh2nZ2cbkNKP2aoiu03Grgnbqz5ferdUq3KjcIoo5zwFe7gPE8G2yaVSdS8Z6r3sbpqQYE7XkNf4niGFOBzORwed_gZswxdz4pJBkuT4EaYbEDjIxpBRte0gZQTUPgtqo2W81-63mQVB3g6Bus_Tp7yX_Eu6dz8CLPaCT0b7N9huXDhxGsGZUoMy92XHfnbfb_05AXV6ez6esf7bTtzoovYr5j-srPE8iolxIychPfx_acQ</recordid><startdate>200901</startdate><enddate>200901</enddate><creator>Lipscomb, Gina L</creator><creator>Keese, Annette M</creator><creator>Cowart, Darin M</creator><creator>Schut, Gerrit J</creator><creator>Thomm, Michael</creator><creator>Adams, Michael W.W</creator><creator>Scott, Robert A</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200901</creationdate><title>SurR: a transcriptional activator and repressor controlling hydrogen and elemental sulphur metabolism in Pyrococcus furiosus</title><author>Lipscomb, Gina L ; Keese, Annette M ; Cowart, Darin M ; Schut, Gerrit J ; Thomm, Michael ; Adams, Michael W.W ; Scott, Robert A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5845-5bec795c153c0d68115094c760367903df7c86e3a569c4e3891116dd85e20f343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Archaeal Proteins - genetics</topic><topic>Archaeal Proteins - metabolism</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Binding sites</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Footprinting</topic><topic>DNA, Archaeal - genetics</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Electrophoretic Mobility Shift Assay</topic><topic>Experiments</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Archaeal</topic><topic>Genes, Archaeal</topic><topic>Genes, Regulator</topic><topic>Hydrogen - metabolism</topic><topic>Microbiology</topic><topic>Open Reading Frames</topic><topic>Operon</topic><topic>Promoter Regions, Genetic</topic><topic>Proteins</topic><topic>Pyrococcus furiosus</topic><topic>Pyrococcus furiosus - genetics</topic><topic>Pyrococcus furiosus - metabolism</topic><topic>Sulfur - metabolism</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lipscomb, Gina L</creatorcontrib><creatorcontrib>Keese, Annette M</creatorcontrib><creatorcontrib>Cowart, Darin M</creatorcontrib><creatorcontrib>Schut, Gerrit J</creatorcontrib><creatorcontrib>Thomm, Michael</creatorcontrib><creatorcontrib>Adams, Michael W.W</creatorcontrib><creatorcontrib>Scott, Robert A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lipscomb, Gina L</au><au>Keese, Annette M</au><au>Cowart, Darin M</au><au>Schut, Gerrit J</au><au>Thomm, Michael</au><au>Adams, Michael W.W</au><au>Scott, Robert A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SurR: a transcriptional activator and repressor controlling hydrogen and elemental sulphur metabolism in Pyrococcus furiosus</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2009-01</date><risdate>2009</risdate><volume>71</volume><issue>2</issue><spage>332</spage><epage>349</epage><pages>332-349</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>This work describes the identification and characterization of SurR, Pyrococcus furiosus sulphur (S⁰) response regulator. SurR was captured from cell extract using promoter DNA of a hydrogenase operon that is downregulated in the primary response of P. furiosus to S⁰, as revealed by DNA microarray experiments. SurR was validated as a sequence-specific DNA binding protein, and characterization of the SurR DNA binding motif GTTn₃AAC led to the identification of several target genes that contain an extended motif in their promoters. A number of these were validated to contain upstream SurR binding sites. These SurR targets strongly correspond with open reading frames and operons both up- and downregulated in the primary response to S⁰. In vitro transcription revealed that SurR is an activator for its own gene as well as for two hydrogenase operons whose expression is downregulated during the primary S⁰ response; it is also a repressor for two genes upregulated during the primary S⁰ response, one of which encodes the primary S⁰-reducing enzyme NAD(P)H sulphur reductase. Herein we give evidence for the role of SurR in both mediating the primary response to S⁰ and controlling hydrogen production in P. furiosus.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19017274</pmid><doi>10.1111/j.1365-2958.2008.06525.x</doi><tpages>18</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Archaeal Proteins - genetics Archaeal Proteins - metabolism Bacteria Bacteriology Binding sites Biological and medical sciences Cloning, Molecular Deoxyribonucleic acid DNA DNA Footprinting DNA, Archaeal - genetics DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Electrophoretic Mobility Shift Assay Experiments Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Archaeal Genes, Archaeal Genes, Regulator Hydrogen - metabolism Microbiology Open Reading Frames Operon Promoter Regions, Genetic Proteins Pyrococcus furiosus Pyrococcus furiosus - genetics Pyrococcus furiosus - metabolism Sulfur - metabolism Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Transcriptional Activation |
| title | SurR: a transcriptional activator and repressor controlling hydrogen and elemental sulphur metabolism in Pyrococcus furiosus |
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