A role for antimicrobial peptides in intestinal microsporidiosis
Clinical isolates from 3 microsporidia species, Encephalitozoon intestinalis and Encephalitozoon hellem, and the insect parasite Anncaliia (Brachiola, Nosema) algerae, were used in spore germination and enterocyte-like (C2Bbe1) cell infection assays to determine the effect of a panel of antimicrobia...
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| Veröffentlicht in: | Parasitology 2009-02, Vol.136 (2), p.175-181 |
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| description | Clinical isolates from 3 microsporidia species, Encephalitozoon intestinalis and Encephalitozoon hellem, and the insect parasite Anncaliia (Brachiola, Nosema) algerae, were used in spore germination and enterocyte-like (C2Bbe1) cell infection assays to determine the effect of a panel of antimicrobial peptides. Spores were incubated with lactoferrin (Lf), lysozyme (Lz), and human beta defensin 2 (HBD2), human alpha defensin 5 (HD5), and human alpha defensin 1 (HNP1), alone and in combination with Lz, prior to germination. Of the Encephalitozoon species only E. hellem spore germination was inhibited by HNP1, while A. algerae spore germination was inhibited by Lf, HBD2, HD5 and HNP1, although HBD2 and HD5 inhibition required the presence of Lz. The effects of HBD2 and HD5 on microsporidia enterocyte infection paralleled their effects on spore germination. Lysozyme alone only inhibited infection with A. algerae, while Lf inhibited infection by E. intestinalis and A. algerae. HNP1 significantly reduced enterocyte infection by all 3 parasite species and a combination of Lf, Lz and HNP1 caused a further reduced infection with A. algerae. These data suggest that intestinal antimicrobial peptides contribute to the defence of the intestine against infection by luminal microsporidia spores and may partially determine which parasite species infects the intestine. |
| doi_str_mv | 10.1017/S0031182008005313 |
| format | Article |
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J. ; CEBALLOS, C.</creator><creatorcontrib>LEITCH, G. J. ; CEBALLOS, C.</creatorcontrib><description>Clinical isolates from 3 microsporidia species, Encephalitozoon intestinalis and Encephalitozoon hellem, and the insect parasite Anncaliia (Brachiola, Nosema) algerae, were used in spore germination and enterocyte-like (C2Bbe1) cell infection assays to determine the effect of a panel of antimicrobial peptides. Spores were incubated with lactoferrin (Lf), lysozyme (Lz), and human beta defensin 2 (HBD2), human alpha defensin 5 (HD5), and human alpha defensin 1 (HNP1), alone and in combination with Lz, prior to germination. Of the Encephalitozoon species only E. hellem spore germination was inhibited by HNP1, while A. algerae spore germination was inhibited by Lf, HBD2, HD5 and HNP1, although HBD2 and HD5 inhibition required the presence of Lz. The effects of HBD2 and HD5 on microsporidia enterocyte infection paralleled their effects on spore germination. Lysozyme alone only inhibited infection with A. algerae, while Lf inhibited infection by E. intestinalis and A. algerae. HNP1 significantly reduced enterocyte infection by all 3 parasite species and a combination of Lf, Lz and HNP1 caused a further reduced infection with A. algerae. These data suggest that intestinal antimicrobial peptides contribute to the defence of the intestine against infection by luminal microsporidia spores and may partially determine which parasite species infects the intestine.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182008005313</identifier><identifier>PMID: 19079820</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>alpha-Defensins - pharmacology ; Anncaliia algerae ; Antifungal Agents - pharmacology ; beta-Defensins - pharmacology ; Biological and medical sciences ; Caco-2 Cells ; Encephalitozoon ; Encephalitozoon - drug effects ; Encephalitozoon - physiology ; Encephalitozoon hellem ; Encephalitozoon intestinalis ; Enterocytes - drug effects ; Enterocytes - microbiology ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; human alpha defensin 5 ; human beta defensin 2 ; human neutrophil peptide 1 ; Humans ; Invertebrates ; lactoferricin B ; lactoferrin ; Lactoferrin - pharmacology ; lysozyme ; Microbial Sensitivity Tests ; Microsporidia ; Microsporidiosis - microbiology ; Muramidase - pharmacology ; Nosema - drug effects ; Nosema - physiology ; Nosema algerae ; Parasites ; Peptides ; Peptides - pharmacology ; Spores, Fungal - drug effects ; Spores, Fungal - physiology</subject><ispartof>Parasitology, 2009-02, Vol.136 (2), p.175-181</ispartof><rights>Copyright © 2008 Cambridge University Press</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c615t-a97eaed002db29f3a270a97670b11128df798f2cb34d7fdb9e9dbc412d42168a3</citedby><cites>FETCH-LOGICAL-c615t-a97eaed002db29f3a270a97670b11128df798f2cb34d7fdb9e9dbc412d42168a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182008005313/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,230,314,776,780,881,27901,27902,55603</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21199474$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19079820$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LEITCH, G. J.</creatorcontrib><creatorcontrib>CEBALLOS, C.</creatorcontrib><title>A role for antimicrobial peptides in intestinal microsporidiosis</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Clinical isolates from 3 microsporidia species, Encephalitozoon intestinalis and Encephalitozoon hellem, and the insect parasite Anncaliia (Brachiola, Nosema) algerae, were used in spore germination and enterocyte-like (C2Bbe1) cell infection assays to determine the effect of a panel of antimicrobial peptides. Spores were incubated with lactoferrin (Lf), lysozyme (Lz), and human beta defensin 2 (HBD2), human alpha defensin 5 (HD5), and human alpha defensin 1 (HNP1), alone and in combination with Lz, prior to germination. Of the Encephalitozoon species only E. hellem spore germination was inhibited by HNP1, while A. algerae spore germination was inhibited by Lf, HBD2, HD5 and HNP1, although HBD2 and HD5 inhibition required the presence of Lz. The effects of HBD2 and HD5 on microsporidia enterocyte infection paralleled their effects on spore germination. Lysozyme alone only inhibited infection with A. algerae, while Lf inhibited infection by E. intestinalis and A. algerae. HNP1 significantly reduced enterocyte infection by all 3 parasite species and a combination of Lf, Lz and HNP1 caused a further reduced infection with A. algerae. These data suggest that intestinal antimicrobial peptides contribute to the defence of the intestine against infection by luminal microsporidia spores and may partially determine which parasite species infects the intestine.</description><subject>alpha-Defensins - pharmacology</subject><subject>Anncaliia algerae</subject><subject>Antifungal Agents - pharmacology</subject><subject>beta-Defensins - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Caco-2 Cells</subject><subject>Encephalitozoon</subject><subject>Encephalitozoon - drug effects</subject><subject>Encephalitozoon - physiology</subject><subject>Encephalitozoon hellem</subject><subject>Encephalitozoon intestinalis</subject><subject>Enterocytes - drug effects</subject><subject>Enterocytes - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>human alpha defensin 5</subject><subject>human beta defensin 2</subject><subject>human neutrophil peptide 1</subject><subject>Humans</subject><subject>Invertebrates</subject><subject>lactoferricin B</subject><subject>lactoferrin</subject><subject>Lactoferrin - pharmacology</subject><subject>lysozyme</subject><subject>Microbial Sensitivity Tests</subject><subject>Microsporidia</subject><subject>Microsporidiosis - microbiology</subject><subject>Muramidase - pharmacology</subject><subject>Nosema - drug effects</subject><subject>Nosema - physiology</subject><subject>Nosema algerae</subject><subject>Parasites</subject><subject>Peptides</subject><subject>Peptides - pharmacology</subject><subject>Spores, Fungal - drug effects</subject><subject>Spores, Fungal - physiology</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkdFr1TAUxsNQtuvVP2AvUgR9q56TtEnzMhzDTXEwxtXnkDbpzNY2NekV_e9NveVuKiIEAuf7ncP5zkfIMcJrBBRvNgAMsaIAFUDJkB2QFRZc5hVyfERWs5zP-hF5EuMtAHDG6SE5QglCpvqKvD3Ngu9s1vqQ6WFyvWuCr53ustGOkzM2Zm5Ib7JxckMq_wLi6IMzzkcXn5LHre6ifbb8a_L5_N2ns_f55dXFh7PTy7zhWE65lsJqawCoqalsmaYCUo0LqBGRVqZNC7W0qVlhRGtqaaWpmwKpKSjySrM1OdnNHbd1b01jhynoTo3B9Tr8UF479bsyuC_qxn9TVBSs5JAGvFoGBP91m-yo3sXGdp0erN9GxXlFpcTqvyAFxgpgIoEv_gBv_TakIyUmxQCikjJBuIPms8Vg2_3KCGpOUf2VYup5_tDrfccSWwJeLoCOje7aoIfGxT1HEaUsku81yXeci5P9vtd1uFNcMFEqfnGtSk4_luebjZp5tiyr-zolfGPvLf173Z927cLI</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>LEITCH, G. J.</creator><creator>CEBALLOS, C.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7T7</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090201</creationdate><title>A role for antimicrobial peptides in intestinal microsporidiosis</title><author>LEITCH, G. J. ; CEBALLOS, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c615t-a97eaed002db29f3a270a97670b11128df798f2cb34d7fdb9e9dbc412d42168a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>alpha-Defensins - pharmacology</topic><topic>Anncaliia algerae</topic><topic>Antifungal Agents - pharmacology</topic><topic>beta-Defensins - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Caco-2 Cells</topic><topic>Encephalitozoon</topic><topic>Encephalitozoon - drug effects</topic><topic>Encephalitozoon - physiology</topic><topic>Encephalitozoon hellem</topic><topic>Encephalitozoon intestinalis</topic><topic>Enterocytes - drug effects</topic><topic>Enterocytes - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>human alpha defensin 5</topic><topic>human beta defensin 2</topic><topic>human neutrophil peptide 1</topic><topic>Humans</topic><topic>Invertebrates</topic><topic>lactoferricin B</topic><topic>lactoferrin</topic><topic>Lactoferrin - pharmacology</topic><topic>lysozyme</topic><topic>Microbial Sensitivity Tests</topic><topic>Microsporidia</topic><topic>Microsporidiosis - microbiology</topic><topic>Muramidase - pharmacology</topic><topic>Nosema - drug effects</topic><topic>Nosema - physiology</topic><topic>Nosema algerae</topic><topic>Parasites</topic><topic>Peptides</topic><topic>Peptides - pharmacology</topic><topic>Spores, Fungal - drug effects</topic><topic>Spores, Fungal - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LEITCH, G. 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J.</au><au>CEBALLOS, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A role for antimicrobial peptides in intestinal microsporidiosis</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>136</volume><issue>2</issue><spage>175</spage><epage>181</epage><pages>175-181</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>Clinical isolates from 3 microsporidia species, Encephalitozoon intestinalis and Encephalitozoon hellem, and the insect parasite Anncaliia (Brachiola, Nosema) algerae, were used in spore germination and enterocyte-like (C2Bbe1) cell infection assays to determine the effect of a panel of antimicrobial peptides. Spores were incubated with lactoferrin (Lf), lysozyme (Lz), and human beta defensin 2 (HBD2), human alpha defensin 5 (HD5), and human alpha defensin 1 (HNP1), alone and in combination with Lz, prior to germination. Of the Encephalitozoon species only E. hellem spore germination was inhibited by HNP1, while A. algerae spore germination was inhibited by Lf, HBD2, HD5 and HNP1, although HBD2 and HD5 inhibition required the presence of Lz. The effects of HBD2 and HD5 on microsporidia enterocyte infection paralleled their effects on spore germination. Lysozyme alone only inhibited infection with A. algerae, while Lf inhibited infection by E. intestinalis and A. algerae. HNP1 significantly reduced enterocyte infection by all 3 parasite species and a combination of Lf, Lz and HNP1 caused a further reduced infection with A. algerae. These data suggest that intestinal antimicrobial peptides contribute to the defence of the intestine against infection by luminal microsporidia spores and may partially determine which parasite species infects the intestine.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>19079820</pmid><doi>10.1017/S0031182008005313</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | alpha-Defensins - pharmacology Anncaliia algerae Antifungal Agents - pharmacology beta-Defensins - pharmacology Biological and medical sciences Caco-2 Cells Encephalitozoon Encephalitozoon - drug effects Encephalitozoon - physiology Encephalitozoon hellem Encephalitozoon intestinalis Enterocytes - drug effects Enterocytes - microbiology Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models human alpha defensin 5 human beta defensin 2 human neutrophil peptide 1 Humans Invertebrates lactoferricin B lactoferrin Lactoferrin - pharmacology lysozyme Microbial Sensitivity Tests Microsporidia Microsporidiosis - microbiology Muramidase - pharmacology Nosema - drug effects Nosema - physiology Nosema algerae Parasites Peptides Peptides - pharmacology Spores, Fungal - drug effects Spores, Fungal - physiology |
| title | A role for antimicrobial peptides in intestinal microsporidiosis |
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