Patterns and Dynamics of Subventricular Zone Neuroblast Migration in the Ischemic Striatum of the Adult Mouse
The migratory behavior of neuroblasts after a stroke is poorly understood. Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that w...
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| Veröffentlicht in: | Journal of cerebral blood flow and metabolism 2009-07, Vol.29 (7), p.1240-1250 |
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| container_title | Journal of cerebral blood flow and metabolism |
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| creator | Zhang, Rui L Chopp, Michael Gregg, Sara R Toh, Yier Roberts, Cindi LeTourneau, Yvonne Buller, Benjamin Jia, Longfei Davarani, Siamak P Nejad Zhang, Zheng G |
| description | The migratory behavior of neuroblasts after a stroke is poorly understood. Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that were subjected to 7 days of stroke. Our results show that neuroblasts originating in the subventricular zone (SVZ) of adult mouse brain laterally migrated in chains or individually to reach the ischemic striatum. The chains were initially formed at the border between the SVZ and the striatum by neuroblasts in the SVZ and then extended to the striatum. The average speed of DCX-eGFP-expressing cells within chains was 28.67 ± 1.04 μm/h, which was significantly faster (P < 0.01) than the speed of the cells in the SVZ (17.98 ± 0.57 μm/h). Within the ischemic striatum, individual neuroblasts actively extended or retracted their processes, suggestive of probing the immediate microenvironment. The neuroblasts close to cerebral blood vessels exhibited multiple processes. Our data suggest that neuroblasts actively interact with the microenvironment to reach the ischemic striatum by multiple migratory routes. |
| doi_str_mv | 10.1038/jcbfm.2009.55 |
| format | Article |
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Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that were subjected to 7 days of stroke. Our results show that neuroblasts originating in the subventricular zone (SVZ) of adult mouse brain laterally migrated in chains or individually to reach the ischemic striatum. The chains were initially formed at the border between the SVZ and the striatum by neuroblasts in the SVZ and then extended to the striatum. The average speed of DCX-eGFP-expressing cells within chains was 28.67 ± 1.04 μm/h, which was significantly faster (P < 0.01) than the speed of the cells in the SVZ (17.98 ± 0.57 μm/h). Within the ischemic striatum, individual neuroblasts actively extended or retracted their processes, suggestive of probing the immediate microenvironment. The neuroblasts close to cerebral blood vessels exhibited multiple processes. Our data suggest that neuroblasts actively interact with the microenvironment to reach the ischemic striatum by multiple migratory routes.</description><identifier>ISSN: 0271-678X</identifier><identifier>EISSN: 1559-7016</identifier><identifier>DOI: 10.1038/jcbfm.2009.55</identifier><identifier>PMID: 19436318</identifier><identifier>CODEN: JCBMDN</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Biological and medical sciences ; Blood. Blood coagulation. Reticuloendothelial system ; Brain Ischemia - pathology ; Cell Movement ; Cerebral Ventricles - pathology ; Cerebrovascular Circulation ; Corpus Striatum - pathology ; Kinetics ; Medical sciences ; Mice ; Microscopy, Video ; Neurology ; Neurons - cytology ; Neurons - physiology ; Pharmacology. Drug treatments ; Stem Cells - cytology ; Stem Cells - physiology ; Stroke - pathology ; Vascular diseases and vascular malformations of the nervous system</subject><ispartof>Journal of cerebral blood flow and metabolism, 2009-07, Vol.29 (7), p.1240-1250</ispartof><rights>2009 ISCBFM</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Jul 2009</rights><rights>2009 ISCBFM All rights reserved 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c574t-1138abcbd3252e83e365072dca3ae9bed1373cfff06731ed3dd3941ae03f06d3</citedby><cites>FETCH-LOGICAL-c574t-1138abcbd3252e83e365072dca3ae9bed1373cfff06731ed3dd3941ae03f06d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1038/jcbfm.2009.55$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1038/jcbfm.2009.55$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>230,314,776,780,881,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21726510$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19436318$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Rui L</creatorcontrib><creatorcontrib>Chopp, Michael</creatorcontrib><creatorcontrib>Gregg, Sara R</creatorcontrib><creatorcontrib>Toh, Yier</creatorcontrib><creatorcontrib>Roberts, Cindi</creatorcontrib><creatorcontrib>LeTourneau, Yvonne</creatorcontrib><creatorcontrib>Buller, Benjamin</creatorcontrib><creatorcontrib>Jia, Longfei</creatorcontrib><creatorcontrib>Davarani, Siamak P Nejad</creatorcontrib><creatorcontrib>Zhang, Zheng G</creatorcontrib><title>Patterns and Dynamics of Subventricular Zone Neuroblast Migration in the Ischemic Striatum of the Adult Mouse</title><title>Journal of cerebral blood flow and metabolism</title><addtitle>J Cereb Blood Flow Metab</addtitle><description>The migratory behavior of neuroblasts after a stroke is poorly understood. Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that were subjected to 7 days of stroke. Our results show that neuroblasts originating in the subventricular zone (SVZ) of adult mouse brain laterally migrated in chains or individually to reach the ischemic striatum. The chains were initially formed at the border between the SVZ and the striatum by neuroblasts in the SVZ and then extended to the striatum. The average speed of DCX-eGFP-expressing cells within chains was 28.67 ± 1.04 μm/h, which was significantly faster (P < 0.01) than the speed of the cells in the SVZ (17.98 ± 0.57 μm/h). Within the ischemic striatum, individual neuroblasts actively extended or retracted their processes, suggestive of probing the immediate microenvironment. The neuroblasts close to cerebral blood vessels exhibited multiple processes. Our data suggest that neuroblasts actively interact with the microenvironment to reach the ischemic striatum by multiple migratory routes.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood. Blood coagulation. Reticuloendothelial system</subject><subject>Brain Ischemia - pathology</subject><subject>Cell Movement</subject><subject>Cerebral Ventricles - pathology</subject><subject>Cerebrovascular Circulation</subject><subject>Corpus Striatum - pathology</subject><subject>Kinetics</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Microscopy, Video</subject><subject>Neurology</subject><subject>Neurons - cytology</subject><subject>Neurons - physiology</subject><subject>Pharmacology. Drug treatments</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - physiology</subject><subject>Stroke - pathology</subject><subject>Vascular diseases and vascular malformations of the nervous system</subject><issn>0271-678X</issn><issn>1559-7016</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkcuLFDEQxoMo7jp69CpB0IPQYx6dTvdFWNbXwvqA3YN4CdVJ9UwP3cmadC_sf2_aGXZVBE-Bql99qa8-Qp5ytuZM1q93tu3GtWCsWSt1jxxzpZpCM17dJ8dMaF5Uuv52RB6ltGOM1VKph-SIN6WsJK-PyfgVpgmjTxS8o29vPIy9TTR09GJur9FPsbfzAJF-Dx7pZ5xjaAdIE_3UbyJMffC093TaIj1Ldot5mF7kGZjmcRFZGiduHjIf5oSPyYMOhoRPDu-KXL5_d3n6sTj_8uHs9OS8sEqXU8G5rKG1rZNCCawlykoxLZwFCdi06LjU0nZdxyotOTrpnGxKDshkLjm5Im_2sldzO6Kziw0YzFXsR4g3JkBv_uz4fms24doIXXJeySzw8iAQw48Z02TGPlkcBvCYfZhK5_uVpfgvKFgjtCx5Bp__Be7CHH0-ghG8UYLXbIGKPWRjSClid7syZ2ZJ2_xK2yxpG6Uy_-x3n3f0Id4MvDgAkCwMXQRv-3TLCa5FpbLwirzacwk2eLfZv3_9CSYBwuY</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>Zhang, Rui L</creator><creator>Chopp, Michael</creator><creator>Gregg, Sara R</creator><creator>Toh, Yier</creator><creator>Roberts, Cindi</creator><creator>LeTourneau, Yvonne</creator><creator>Buller, Benjamin</creator><creator>Jia, Longfei</creator><creator>Davarani, Siamak P Nejad</creator><creator>Zhang, Zheng G</creator><general>SAGE Publications</general><general>Nature Publishing Group</general><general>Sage Publications Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090701</creationdate><title>Patterns and Dynamics of Subventricular Zone Neuroblast Migration in the Ischemic Striatum of the Adult Mouse</title><author>Zhang, Rui L ; Chopp, Michael ; Gregg, Sara R ; Toh, Yier ; Roberts, Cindi ; LeTourneau, Yvonne ; Buller, Benjamin ; Jia, Longfei ; Davarani, Siamak P Nejad ; Zhang, Zheng G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c574t-1138abcbd3252e83e365072dca3ae9bed1373cfff06731ed3dd3941ae03f06d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood. Blood coagulation. Reticuloendothelial system</topic><topic>Brain Ischemia - pathology</topic><topic>Cell Movement</topic><topic>Cerebral Ventricles - pathology</topic><topic>Cerebrovascular Circulation</topic><topic>Corpus Striatum - pathology</topic><topic>Kinetics</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Microscopy, Video</topic><topic>Neurology</topic><topic>Neurons - cytology</topic><topic>Neurons - physiology</topic><topic>Pharmacology. Drug treatments</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - physiology</topic><topic>Stroke - pathology</topic><topic>Vascular diseases and vascular malformations of the nervous system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Rui L</creatorcontrib><creatorcontrib>Chopp, Michael</creatorcontrib><creatorcontrib>Gregg, Sara R</creatorcontrib><creatorcontrib>Toh, Yier</creatorcontrib><creatorcontrib>Roberts, Cindi</creatorcontrib><creatorcontrib>LeTourneau, Yvonne</creatorcontrib><creatorcontrib>Buller, Benjamin</creatorcontrib><creatorcontrib>Jia, Longfei</creatorcontrib><creatorcontrib>Davarani, Siamak P Nejad</creatorcontrib><creatorcontrib>Zhang, Zheng G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of cerebral blood flow and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Rui L</au><au>Chopp, Michael</au><au>Gregg, Sara R</au><au>Toh, Yier</au><au>Roberts, Cindi</au><au>LeTourneau, Yvonne</au><au>Buller, Benjamin</au><au>Jia, Longfei</au><au>Davarani, Siamak P Nejad</au><au>Zhang, Zheng G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Patterns and Dynamics of Subventricular Zone Neuroblast Migration in the Ischemic Striatum of the Adult Mouse</atitle><jtitle>Journal of cerebral blood flow and metabolism</jtitle><addtitle>J Cereb Blood Flow Metab</addtitle><date>2009-07-01</date><risdate>2009</risdate><volume>29</volume><issue>7</issue><spage>1240</spage><epage>1250</epage><pages>1240-1250</pages><issn>0271-678X</issn><eissn>1559-7016</eissn><coden>JCBMDN</coden><abstract>The migratory behavior of neuroblasts after a stroke is poorly understood. Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that were subjected to 7 days of stroke. Our results show that neuroblasts originating in the subventricular zone (SVZ) of adult mouse brain laterally migrated in chains or individually to reach the ischemic striatum. The chains were initially formed at the border between the SVZ and the striatum by neuroblasts in the SVZ and then extended to the striatum. The average speed of DCX-eGFP-expressing cells within chains was 28.67 ± 1.04 μm/h, which was significantly faster (P < 0.01) than the speed of the cells in the SVZ (17.98 ± 0.57 μm/h). Within the ischemic striatum, individual neuroblasts actively extended or retracted their processes, suggestive of probing the immediate microenvironment. The neuroblasts close to cerebral blood vessels exhibited multiple processes. Our data suggest that neuroblasts actively interact with the microenvironment to reach the ischemic striatum by multiple migratory routes.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>19436318</pmid><doi>10.1038/jcbfm.2009.55</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0271-678X |
| ispartof | Journal of cerebral blood flow and metabolism, 2009-07, Vol.29 (7), p.1240-1250 |
| issn | 0271-678X 1559-7016 |
| language | eng |
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| source | SAGE Complete A-Z List; MEDLINE |
| subjects | Animals Biological and medical sciences Blood. Blood coagulation. Reticuloendothelial system Brain Ischemia - pathology Cell Movement Cerebral Ventricles - pathology Cerebrovascular Circulation Corpus Striatum - pathology Kinetics Medical sciences Mice Microscopy, Video Neurology Neurons - cytology Neurons - physiology Pharmacology. Drug treatments Stem Cells - cytology Stem Cells - physiology Stroke - pathology Vascular diseases and vascular malformations of the nervous system |
| title | Patterns and Dynamics of Subventricular Zone Neuroblast Migration in the Ischemic Striatum of the Adult Mouse |
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