Stable generation of serum- and feeder-free embryonic stem cell-derived mice with full germline-competency by using a GSK3 specific inhibitor

C57BL/6 (B6)‐derived embryonic stem (ES) cells are not widely used to generate knockout mice despite the advantage of a well‐defined genetic background because of poor developmental potential. We newly established serum‐ and feeder‐free B6 ES cells with full developmental potential by using leukemia...

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Veröffentlicht in:	Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2009-06, Vol.47 (6), p.414-422
Hauptverfasser:	Sato, Hiromu, Amagai, Keiko, Shimizukawa, Rie, Tamai, Yoshitaka
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals BIO C57BL/6 Cell Culture Techniques Cells, Cultured Culture Media, Serum-Free - pharmacology Embryo, Mammalian - cytology Embryo, Mammalian - drug effects Embryo, Mammalian - metabolism embryonic stem cell Embryonic Stem Cells - cytology Embryonic Stem Cells - drug effects Embryonic Stem Cells - metabolism feeder free Female Gene Expression Regulation, Developmental - drug effects Glycogen Synthase Kinase 3 - antagonists & inhibitors Glycogen Synthase Kinase 3 - metabolism Indoles - pharmacology Leukemia Inhibitory Factor - pharmacology Male Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, Inbred Strains Mice, Knockout Oligonucleotide Array Sequence Analysis Oximes - pharmacology Pluripotent Stem Cells - cytology Pluripotent Stem Cells - drug effects Pluripotent Stem Cells - metabolism Receptors, Leptin - genetics Receptors, Leptin - metabolism serum free Totipotent Stem Cells - cytology Totipotent Stem Cells - drug effects Totipotent Stem Cells - metabolism
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