Comparative Methylation of ERVWE1/Syncytin-1 and Other Human Endogenous Retrovirus LTRs in Placenta Tissues
Human endogenous retroviruses (HERVs) are globally silent in somatic cells. However, some HERVs display high transcription in physiological conditions. In particular, ERVWE1, ERVFRDE1 and ERV3, three proviruses of distinct families, are highly transcribed in placenta and produce envelope proteins as...
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| Veröffentlicht in: | DNA research 2009-08, Vol.16 (4), p.195-211 |
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| creator | Gimenez, Juliette Montgiraud, Cécile Oriol, Guy Pichon, Jean-Philippe Ruel, Karine Tsatsaris, Vassilis Gerbaud, Pascale Frendo, Jean-Louis Evain-Brion, Danièle Mallet, François |
| description | Human endogenous retroviruses (HERVs) are globally silent in somatic cells. However, some HERVs display high transcription in physiological conditions. In particular, ERVWE1, ERVFRDE1 and ERV3, three proviruses of distinct families, are highly transcribed in placenta and produce envelope proteins associated with placenta development. As silencing of repeated elements is thought to occur mainly by DNA methylation, we compared the methylation of ERVWE1 and related HERVs to appreciate whether HERV methylation relies upon the family, the integration site, the tissue, the long terminal repeat (LTR) function or the associated gene function. CpG methylation of HERV-W LTRs in placenta-associated tissues was heterogeneous but a joint epigenetic control was found for ERVWE1 5′LTR and its juxtaposed enhancer, a mammalian apparent LTR retrotransposon. Additionally, ERVWE1, ERVFRDE1 and ERV3 5′LTRs were all essentially hypomethylated in cytotrophoblasts during pregnancy, but showed distinct and stage-dependent methylation profiles. In non-cytotrophoblastic cells, they also exhibited different methylation profiles, compatible with their respective transcriptional activities. Comparative analyses of transcriptional activity and LTR methylation in cell lines further sustained a role for methylation in the control of functional LTRs. These results suggest that HERV methylation might not be family related but copy-specific, and related to the LTR function and the tissue. In particular, ERVWE1 and ERV3 could be developmentally epigenetically regulated HERVs. |
| doi_str_mv | 10.1093/dnares/dsp011 |
| format | Article |
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However, some HERVs display high transcription in physiological conditions. In particular, ERVWE1, ERVFRDE1 and ERV3, three proviruses of distinct families, are highly transcribed in placenta and produce envelope proteins associated with placenta development. As silencing of repeated elements is thought to occur mainly by DNA methylation, we compared the methylation of ERVWE1 and related HERVs to appreciate whether HERV methylation relies upon the family, the integration site, the tissue, the long terminal repeat (LTR) function or the associated gene function. CpG methylation of HERV-W LTRs in placenta-associated tissues was heterogeneous but a joint epigenetic control was found for ERVWE1 5′LTR and its juxtaposed enhancer, a mammalian apparent LTR retrotransposon. Additionally, ERVWE1, ERVFRDE1 and ERV3 5′LTRs were all essentially hypomethylated in cytotrophoblasts during pregnancy, but showed distinct and stage-dependent methylation profiles. In non-cytotrophoblastic cells, they also exhibited different methylation profiles, compatible with their respective transcriptional activities. Comparative analyses of transcriptional activity and LTR methylation in cell lines further sustained a role for methylation in the control of functional LTRs. These results suggest that HERV methylation might not be family related but copy-specific, and related to the LTR function and the tissue. In particular, ERVWE1 and ERV3 could be developmentally epigenetically regulated HERVs.</description><identifier>ISSN: 1340-2838</identifier><identifier>EISSN: 1756-1663</identifier><identifier>DOI: 10.1093/dnares/dsp011</identifier><identifier>PMID: 19561344</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Base Sequence ; Biological and medical sciences ; Cell Line, Tumor ; Cells, Cultured ; CpG Islands ; DNA Methylation ; Endogenous Retroviruses - genetics ; Endogenous Retroviruses - metabolism ; Female ; Fibroblasts - cytology ; Fibroblasts - virology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Gene Products, env - genetics ; Gene Products, env - metabolism ; Genes. Genome ; Genetics of eukaryotes. Biological and molecular evolution ; Humans ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Placenta - cytology ; Placenta - metabolism ; Placenta - virology ; Pregnancy Proteins - genetics ; Pregnancy Proteins - metabolism ; Promoter Regions, Genetic ; Terminal Repeat Sequences - genetics</subject><ispartof>DNA research, 2009-08, Vol.16 (4), p.195-211</ispartof><rights>The Author 2009. Kazusa DNA Research Institute 2009</rights><rights>2009 INIST-CNRS</rights><rights>The Author 2009. 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However, some HERVs display high transcription in physiological conditions. In particular, ERVWE1, ERVFRDE1 and ERV3, three proviruses of distinct families, are highly transcribed in placenta and produce envelope proteins associated with placenta development. As silencing of repeated elements is thought to occur mainly by DNA methylation, we compared the methylation of ERVWE1 and related HERVs to appreciate whether HERV methylation relies upon the family, the integration site, the tissue, the long terminal repeat (LTR) function or the associated gene function. CpG methylation of HERV-W LTRs in placenta-associated tissues was heterogeneous but a joint epigenetic control was found for ERVWE1 5′LTR and its juxtaposed enhancer, a mammalian apparent LTR retrotransposon. Additionally, ERVWE1, ERVFRDE1 and ERV3 5′LTRs were all essentially hypomethylated in cytotrophoblasts during pregnancy, but showed distinct and stage-dependent methylation profiles. In non-cytotrophoblastic cells, they also exhibited different methylation profiles, compatible with their respective transcriptional activities. Comparative analyses of transcriptional activity and LTR methylation in cell lines further sustained a role for methylation in the control of functional LTRs. These results suggest that HERV methylation might not be family related but copy-specific, and related to the LTR function and the tissue. In particular, ERVWE1 and ERV3 could be developmentally epigenetically regulated HERVs.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>Cells, Cultured</subject><subject>CpG Islands</subject><subject>DNA Methylation</subject><subject>Endogenous Retroviruses - genetics</subject><subject>Endogenous Retroviruses - metabolism</subject><subject>Female</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - virology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Gene Products, env - genetics</subject><subject>Gene Products, env - metabolism</subject><subject>Genes. Genome</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Placenta - cytology</subject><subject>Placenta - metabolism</subject><subject>Placenta - virology</subject><subject>Pregnancy Proteins - genetics</subject><subject>Pregnancy Proteins - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Terminal Repeat Sequences - genetics</subject><issn>1340-2838</issn><issn>1756-1663</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EoqVw5Ip8oeIS1k6c2L4godWWIm1VtCxwtGZtp2tI7GAnK-2_x1VWBU49zZPm05s3egi9puQ9JbJaGA_RpoVJA6H0CTqnvG4K2jTV06wrRopSVOIMvUjpJyGM1hV_js6orJu8ZOfo1zL0A0QY3cHiGzvuj13WwePQ4tXm-48VXXw9en0cnS8oBm_w7bi3EV9PPXi88ibcWR-mhDd2jOHgYpbr7SZh5_GXDrT1I-CtS2my6SV61kKX7KvTvEDfrlbb5XWxvv30eflxXeiasrEASirDhC4BjGwN37UMpCVlw3bWEGk4MSWRkjNGgHABjGnLGsMktRyEYNUF-jD7DtOut-Y-Q4RODdH1EI8qgFP_b7zbq7twUCUvay5ENrg8GcTwOwcfVe-Stl0H3uZfVcNrzmpKM1jMoI4hpWjbhyOUqPt61FyPmuvJ_Jt_k_2lT31k4O0JgKShayN47dIDV1JR0qaWmXs3c2EaHrn5B-85qgs</recordid><startdate>20090801</startdate><enddate>20090801</enddate><creator>Gimenez, Juliette</creator><creator>Montgiraud, Cécile</creator><creator>Oriol, Guy</creator><creator>Pichon, Jean-Philippe</creator><creator>Ruel, Karine</creator><creator>Tsatsaris, Vassilis</creator><creator>Gerbaud, Pascale</creator><creator>Frendo, Jean-Louis</creator><creator>Evain-Brion, Danièle</creator><creator>Mallet, François</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090801</creationdate><title>Comparative Methylation of ERVWE1/Syncytin-1 and Other Human Endogenous Retrovirus LTRs in Placenta Tissues</title><author>Gimenez, Juliette ; Montgiraud, Cécile ; Oriol, Guy ; Pichon, Jean-Philippe ; Ruel, Karine ; Tsatsaris, Vassilis ; Gerbaud, Pascale ; Frendo, Jean-Louis ; Evain-Brion, Danièle ; Mallet, François</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-a103d48c2aad9fd7bf4a9e0264bed09d70d20997440a078a44ce46d491e7a8843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>Cells, Cultured</topic><topic>CpG Islands</topic><topic>DNA Methylation</topic><topic>Endogenous Retroviruses - genetics</topic><topic>Endogenous Retroviruses - metabolism</topic><topic>Female</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - virology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Gene Products, env - genetics</topic><topic>Gene Products, env - metabolism</topic><topic>Genes. Genome</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Placenta - cytology</topic><topic>Placenta - metabolism</topic><topic>Placenta - virology</topic><topic>Pregnancy Proteins - genetics</topic><topic>Pregnancy Proteins - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>Terminal Repeat Sequences - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gimenez, Juliette</creatorcontrib><creatorcontrib>Montgiraud, Cécile</creatorcontrib><creatorcontrib>Oriol, Guy</creatorcontrib><creatorcontrib>Pichon, Jean-Philippe</creatorcontrib><creatorcontrib>Ruel, Karine</creatorcontrib><creatorcontrib>Tsatsaris, Vassilis</creatorcontrib><creatorcontrib>Gerbaud, Pascale</creatorcontrib><creatorcontrib>Frendo, Jean-Louis</creatorcontrib><creatorcontrib>Evain-Brion, Danièle</creatorcontrib><creatorcontrib>Mallet, François</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>DNA research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Gimenez, Juliette</au><au>Montgiraud, Cécile</au><au>Oriol, Guy</au><au>Pichon, Jean-Philippe</au><au>Ruel, Karine</au><au>Tsatsaris, Vassilis</au><au>Gerbaud, Pascale</au><au>Frendo, Jean-Louis</au><au>Evain-Brion, Danièle</au><au>Mallet, François</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Methylation of ERVWE1/Syncytin-1 and Other Human Endogenous Retrovirus LTRs in Placenta Tissues</atitle><jtitle>DNA research</jtitle><addtitle>DNA Res</addtitle><date>2009-08-01</date><risdate>2009</risdate><volume>16</volume><issue>4</issue><spage>195</spage><epage>211</epage><pages>195-211</pages><issn>1340-2838</issn><eissn>1756-1663</eissn><abstract>Human endogenous retroviruses (HERVs) are globally silent in somatic cells. However, some HERVs display high transcription in physiological conditions. In particular, ERVWE1, ERVFRDE1 and ERV3, three proviruses of distinct families, are highly transcribed in placenta and produce envelope proteins associated with placenta development. As silencing of repeated elements is thought to occur mainly by DNA methylation, we compared the methylation of ERVWE1 and related HERVs to appreciate whether HERV methylation relies upon the family, the integration site, the tissue, the long terminal repeat (LTR) function or the associated gene function. CpG methylation of HERV-W LTRs in placenta-associated tissues was heterogeneous but a joint epigenetic control was found for ERVWE1 5′LTR and its juxtaposed enhancer, a mammalian apparent LTR retrotransposon. Additionally, ERVWE1, ERVFRDE1 and ERV3 5′LTRs were all essentially hypomethylated in cytotrophoblasts during pregnancy, but showed distinct and stage-dependent methylation profiles. In non-cytotrophoblastic cells, they also exhibited different methylation profiles, compatible with their respective transcriptional activities. Comparative analyses of transcriptional activity and LTR methylation in cell lines further sustained a role for methylation in the control of functional LTRs. These results suggest that HERV methylation might not be family related but copy-specific, and related to the LTR function and the tissue. In particular, ERVWE1 and ERV3 could be developmentally epigenetically regulated HERVs.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>19561344</pmid><doi>10.1093/dnares/dsp011</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Base Sequence Biological and medical sciences Cell Line, Tumor Cells, Cultured CpG Islands DNA Methylation Endogenous Retroviruses - genetics Endogenous Retroviruses - metabolism Female Fibroblasts - cytology Fibroblasts - virology Fundamental and applied biological sciences. Psychology Gene Expression Regulation Gene Products, env - genetics Gene Products, env - metabolism Genes. Genome Genetics of eukaryotes. Biological and molecular evolution Humans Molecular and cellular biology Molecular genetics Molecular Sequence Data Placenta - cytology Placenta - metabolism Placenta - virology Pregnancy Proteins - genetics Pregnancy Proteins - metabolism Promoter Regions, Genetic Terminal Repeat Sequences - genetics |
| title | Comparative Methylation of ERVWE1/Syncytin-1 and Other Human Endogenous Retrovirus LTRs in Placenta Tissues |
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