Maggot secretions suppress pro-inflammatory responses of human monocytes through elevation of cyclic AMP
Aims/hypothesis Maggots of the blowfly Lucilia sericata are used for the treatment of chronic wounds. As monocytes may contribute to the excessive inflammatory responses in such wounds, this study focussed on the effects of maggot secretions on the pro-inflammatory activities of these cells. Methods...
Gespeichert in:
| Veröffentlicht in: | Diabetologia 2009-09, Vol.52 (9), p.1962-1970 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1970 |
|---|---|
| container_issue | 9 |
| container_start_page | 1962 |
| container_title | Diabetologia |
| container_volume | 52 |
| creator | van der Plas, M. J. A. Baldry, M. van Dissel, J. T. Jukema, G. N. Nibbering, P. H. |
| description | Aims/hypothesis
Maggots of the blowfly
Lucilia sericata
are used for the treatment of chronic wounds. As monocytes may contribute to the excessive inflammatory responses in such wounds, this study focussed on the effects of maggot secretions on the pro-inflammatory activities of these cells.
Methods
Freshly isolated monocytes were incubated with a range of secretions for 1 h and then stimulated with lipopolysaccharides (range 0–100 ng/ml) or lipoteichoic acid (range 0–5 µg/ml) for 18 h. The expression of cell surface molecules, cytokine and chemokine levels in culture supernatants, cell viability, chemotaxis, and phagocytosis and killing of
Staphylococcus aureus
were measured.
Results
Maggot secretions dose-dependently inhibited production of the pro-inflammatory cytokines TNF-α, IL-12p40 and macrophage migration inhibitory factor by lipopolysaccharides- and lipoteichoic acid-stimulated monocytes, while enhancing production of the anti-inflammatory cytokine IL-10. Expression of cell surface receptors involved in pathogen recognition remained unaffected by secretions. In addition, maggot secretions altered the chemokine profile of monocytes by downregulating macrophage inflammatory protein-1β and upregulating monocyte chemoattractant protein-1 and IL-8. Nevertheless, chemotactic responses of monocytes were inhibited by secretions. Furthermore, maggot secretions did not affect phagocytosis and intracellular killing of
S. aureus
by human monocytes. Finally, secretions induced a transient rise in the intracellular cyclic AMP concentration in monocytes and Rp-cyclic AMPS inhibited the effects of secretions.
Conclusions/interpretation
Maggot secretions inhibit the pro-inflammatory responses of human monocytes through a cyclic AMP-dependent mechanism. Regulation of the inflammatory processes by maggots contributes to their beneficial effects on chronic wounds. |
| doi_str_mv | 10.1007/s00125-009-1432-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2723663</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20776119</sourcerecordid><originalsourceid>FETCH-LOGICAL-c595t-1f36f4b3ae53b0cb74b018fbc3d1c4708e3c7a897abcf516a6a709f37afabafd3</originalsourceid><addsrcrecordid>eNp9kVuL1TAUhYMozvHoD_BFgqA-VXNpkvZFGAZvMIM-KPgWdnOStkOb1KQdOP_edM5hLsL4lLDXt1d29kLoJSXvKSHqQyKEMlEQUhe05KyQj9Dm-kJKVj1Gm1UuaCV_n6BnKV0SQrgo5VN0QmuhBFXVBnUX0LZhxsmaaOc--ITTMk3RpoSnGIreuwHGEeYQ9zhXp0zYhIPD3TKCx2PwweznXJq7GJa2w3awV7A6rZDZm6E3-PTix3P0xMGQ7IvjuUW_Pn_6efa1OP_-5dvZ6XlhRC3mgjouXdlwsII3xDSqbAitXGP4jppSkcpyo6CqFTTGCSpBgiK14wocNOB2fIs-HnynpRntzlg_Rxj0FPsR4l4H6PV9xfedbsOVZopxKXk2eHc0iOHPYtOsxz4ZOwzgbViSVpzXUq273KK3_yUZUUpSWmfw9T_gZViiz2vQjPKqFEqWGaIHyMSQUrTuZmZK9Bq3PsStc9x6TVnL3PPq7mdvO475ZuDNEYBkYHARvOnTDcdoJWpSqsyxA5ey5Fsbbyd8-PW_eZrFvg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213845764</pqid></control><display><type>article</type><title>Maggot secretions suppress pro-inflammatory responses of human monocytes through elevation of cyclic AMP</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>van der Plas, M. J. A. ; Baldry, M. ; van Dissel, J. T. ; Jukema, G. N. ; Nibbering, P. H.</creator><creatorcontrib>van der Plas, M. J. A. ; Baldry, M. ; van Dissel, J. T. ; Jukema, G. N. ; Nibbering, P. H.</creatorcontrib><description>Aims/hypothesis
Maggots of the blowfly
Lucilia sericata
are used for the treatment of chronic wounds. As monocytes may contribute to the excessive inflammatory responses in such wounds, this study focussed on the effects of maggot secretions on the pro-inflammatory activities of these cells.
Methods
Freshly isolated monocytes were incubated with a range of secretions for 1 h and then stimulated with lipopolysaccharides (range 0–100 ng/ml) or lipoteichoic acid (range 0–5 µg/ml) for 18 h. The expression of cell surface molecules, cytokine and chemokine levels in culture supernatants, cell viability, chemotaxis, and phagocytosis and killing of
Staphylococcus aureus
were measured.
Results
Maggot secretions dose-dependently inhibited production of the pro-inflammatory cytokines TNF-α, IL-12p40 and macrophage migration inhibitory factor by lipopolysaccharides- and lipoteichoic acid-stimulated monocytes, while enhancing production of the anti-inflammatory cytokine IL-10. Expression of cell surface receptors involved in pathogen recognition remained unaffected by secretions. In addition, maggot secretions altered the chemokine profile of monocytes by downregulating macrophage inflammatory protein-1β and upregulating monocyte chemoattractant protein-1 and IL-8. Nevertheless, chemotactic responses of monocytes were inhibited by secretions. Furthermore, maggot secretions did not affect phagocytosis and intracellular killing of
S. aureus
by human monocytes. Finally, secretions induced a transient rise in the intracellular cyclic AMP concentration in monocytes and Rp-cyclic AMPS inhibited the effects of secretions.
Conclusions/interpretation
Maggot secretions inhibit the pro-inflammatory responses of human monocytes through a cyclic AMP-dependent mechanism. Regulation of the inflammatory processes by maggots contributes to their beneficial effects on chronic wounds.</description><identifier>ISSN: 0012-186X</identifier><identifier>EISSN: 1432-0428</identifier><identifier>DOI: 10.1007/s00125-009-1432-6</identifier><identifier>PMID: 19575178</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Amputation ; Animals ; Biological and medical sciences ; Calliphoridae ; Cell Movement - drug effects ; Cell Movement - physiology ; Cell Survival - drug effects ; Cells, Cultured ; Chemokines ; Chemokines - secretion ; Chronic illnesses ; Cytokines ; Cytokines - secretion ; Diabetes ; Diabetes. Impaired glucose tolerance ; Diptera ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Etiopathogenesis. Screening. Investigations. Target tissue resistance ; Flow Cytometry ; Foot diseases ; Human Physiology ; Humans ; Inflammation - prevention & control ; Interleukin-10 - pharmacology ; Interleukin-10 - secretion ; Interleukin-10 - therapeutic use ; Internal Medicine ; Larva - drug effects ; Larva - physiology ; Leg ulcers ; Lipopolysaccharides - pharmacology ; Lucilia sericata ; Medical sciences ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Microorganisms ; Monocytes - cytology ; Monocytes - drug effects ; Monocytes - microbiology ; Monocytes - physiology ; Neutrophils ; Phagocytosis - drug effects ; Proteins ; Signal transduction ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Teichoic Acids - pharmacology ; Wounds and Injuries - therapy</subject><ispartof>Diabetologia, 2009-09, Vol.52 (9), p.1962-1970</ispartof><rights>The Author(s) 2009</rights><rights>2009 INIST-CNRS</rights><rights>Springer-Verlag 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c595t-1f36f4b3ae53b0cb74b018fbc3d1c4708e3c7a897abcf516a6a709f37afabafd3</citedby><cites>FETCH-LOGICAL-c595t-1f36f4b3ae53b0cb74b018fbc3d1c4708e3c7a897abcf516a6a709f37afabafd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00125-009-1432-6$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00125-009-1432-6$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21859047$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19575178$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van der Plas, M. J. A.</creatorcontrib><creatorcontrib>Baldry, M.</creatorcontrib><creatorcontrib>van Dissel, J. T.</creatorcontrib><creatorcontrib>Jukema, G. N.</creatorcontrib><creatorcontrib>Nibbering, P. H.</creatorcontrib><title>Maggot secretions suppress pro-inflammatory responses of human monocytes through elevation of cyclic AMP</title><title>Diabetologia</title><addtitle>Diabetologia</addtitle><addtitle>Diabetologia</addtitle><description>Aims/hypothesis
Maggots of the blowfly
Lucilia sericata
are used for the treatment of chronic wounds. As monocytes may contribute to the excessive inflammatory responses in such wounds, this study focussed on the effects of maggot secretions on the pro-inflammatory activities of these cells.
Methods
Freshly isolated monocytes were incubated with a range of secretions for 1 h and then stimulated with lipopolysaccharides (range 0–100 ng/ml) or lipoteichoic acid (range 0–5 µg/ml) for 18 h. The expression of cell surface molecules, cytokine and chemokine levels in culture supernatants, cell viability, chemotaxis, and phagocytosis and killing of
Staphylococcus aureus
were measured.
Results
Maggot secretions dose-dependently inhibited production of the pro-inflammatory cytokines TNF-α, IL-12p40 and macrophage migration inhibitory factor by lipopolysaccharides- and lipoteichoic acid-stimulated monocytes, while enhancing production of the anti-inflammatory cytokine IL-10. Expression of cell surface receptors involved in pathogen recognition remained unaffected by secretions. In addition, maggot secretions altered the chemokine profile of monocytes by downregulating macrophage inflammatory protein-1β and upregulating monocyte chemoattractant protein-1 and IL-8. Nevertheless, chemotactic responses of monocytes were inhibited by secretions. Furthermore, maggot secretions did not affect phagocytosis and intracellular killing of
S. aureus
by human monocytes. Finally, secretions induced a transient rise in the intracellular cyclic AMP concentration in monocytes and Rp-cyclic AMPS inhibited the effects of secretions.
Conclusions/interpretation
Maggot secretions inhibit the pro-inflammatory responses of human monocytes through a cyclic AMP-dependent mechanism. Regulation of the inflammatory processes by maggots contributes to their beneficial effects on chronic wounds.</description><subject>Amputation</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calliphoridae</subject><subject>Cell Movement - drug effects</subject><subject>Cell Movement - physiology</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Chemokines</subject><subject>Chemokines - secretion</subject><subject>Chronic illnesses</subject><subject>Cytokines</subject><subject>Cytokines - secretion</subject><subject>Diabetes</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Diptera</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Etiopathogenesis. Screening. Investigations. Target tissue resistance</subject><subject>Flow Cytometry</subject><subject>Foot diseases</subject><subject>Human Physiology</subject><subject>Humans</subject><subject>Inflammation - prevention & control</subject><subject>Interleukin-10 - pharmacology</subject><subject>Interleukin-10 - secretion</subject><subject>Interleukin-10 - therapeutic use</subject><subject>Internal Medicine</subject><subject>Larva - drug effects</subject><subject>Larva - physiology</subject><subject>Leg ulcers</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Lucilia sericata</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Microorganisms</subject><subject>Monocytes - cytology</subject><subject>Monocytes - drug effects</subject><subject>Monocytes - microbiology</subject><subject>Monocytes - physiology</subject><subject>Neutrophils</subject><subject>Phagocytosis - drug effects</subject><subject>Proteins</subject><subject>Signal transduction</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Teichoic Acids - pharmacology</subject><subject>Wounds and Injuries - therapy</subject><issn>0012-186X</issn><issn>1432-0428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp9kVuL1TAUhYMozvHoD_BFgqA-VXNpkvZFGAZvMIM-KPgWdnOStkOb1KQdOP_edM5hLsL4lLDXt1d29kLoJSXvKSHqQyKEMlEQUhe05KyQj9Dm-kJKVj1Gm1UuaCV_n6BnKV0SQrgo5VN0QmuhBFXVBnUX0LZhxsmaaOc--ITTMk3RpoSnGIreuwHGEeYQ9zhXp0zYhIPD3TKCx2PwweznXJq7GJa2w3awV7A6rZDZm6E3-PTix3P0xMGQ7IvjuUW_Pn_6efa1OP_-5dvZ6XlhRC3mgjouXdlwsII3xDSqbAitXGP4jppSkcpyo6CqFTTGCSpBgiK14wocNOB2fIs-HnynpRntzlg_Rxj0FPsR4l4H6PV9xfedbsOVZopxKXk2eHc0iOHPYtOsxz4ZOwzgbViSVpzXUq273KK3_yUZUUpSWmfw9T_gZViiz2vQjPKqFEqWGaIHyMSQUrTuZmZK9Bq3PsStc9x6TVnL3PPq7mdvO475ZuDNEYBkYHARvOnTDcdoJWpSqsyxA5ey5Fsbbyd8-PW_eZrFvg</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>van der Plas, M. J. A.</creator><creator>Baldry, M.</creator><creator>van Dissel, J. T.</creator><creator>Jukema, G. N.</creator><creator>Nibbering, P. H.</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QL</scope><scope>7SS</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090901</creationdate><title>Maggot secretions suppress pro-inflammatory responses of human monocytes through elevation of cyclic AMP</title><author>van der Plas, M. J. A. ; Baldry, M. ; van Dissel, J. T. ; Jukema, G. N. ; Nibbering, P. H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c595t-1f36f4b3ae53b0cb74b018fbc3d1c4708e3c7a897abcf516a6a709f37afabafd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amputation</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calliphoridae</topic><topic>Cell Movement - drug effects</topic><topic>Cell Movement - physiology</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Chemokines</topic><topic>Chemokines - secretion</topic><topic>Chronic illnesses</topic><topic>Cytokines</topic><topic>Cytokines - secretion</topic><topic>Diabetes</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Diptera</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Etiopathogenesis. Screening. Investigations. Target tissue resistance</topic><topic>Flow Cytometry</topic><topic>Foot diseases</topic><topic>Human Physiology</topic><topic>Humans</topic><topic>Inflammation - prevention & control</topic><topic>Interleukin-10 - pharmacology</topic><topic>Interleukin-10 - secretion</topic><topic>Interleukin-10 - therapeutic use</topic><topic>Internal Medicine</topic><topic>Larva - drug effects</topic><topic>Larva - physiology</topic><topic>Leg ulcers</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Lucilia sericata</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Microorganisms</topic><topic>Monocytes - cytology</topic><topic>Monocytes - drug effects</topic><topic>Monocytes - microbiology</topic><topic>Monocytes - physiology</topic><topic>Neutrophils</topic><topic>Phagocytosis - drug effects</topic><topic>Proteins</topic><topic>Signal transduction</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Teichoic Acids - pharmacology</topic><topic>Wounds and Injuries - therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van der Plas, M. J. A.</creatorcontrib><creatorcontrib>Baldry, M.</creatorcontrib><creatorcontrib>van Dissel, J. T.</creatorcontrib><creatorcontrib>Jukema, G. N.</creatorcontrib><creatorcontrib>Nibbering, P. H.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Diabetologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van der Plas, M. J. A.</au><au>Baldry, M.</au><au>van Dissel, J. T.</au><au>Jukema, G. N.</au><au>Nibbering, P. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maggot secretions suppress pro-inflammatory responses of human monocytes through elevation of cyclic AMP</atitle><jtitle>Diabetologia</jtitle><stitle>Diabetologia</stitle><addtitle>Diabetologia</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>52</volume><issue>9</issue><spage>1962</spage><epage>1970</epage><pages>1962-1970</pages><issn>0012-186X</issn><eissn>1432-0428</eissn><abstract>Aims/hypothesis
Maggots of the blowfly
Lucilia sericata
are used for the treatment of chronic wounds. As monocytes may contribute to the excessive inflammatory responses in such wounds, this study focussed on the effects of maggot secretions on the pro-inflammatory activities of these cells.
Methods
Freshly isolated monocytes were incubated with a range of secretions for 1 h and then stimulated with lipopolysaccharides (range 0–100 ng/ml) or lipoteichoic acid (range 0–5 µg/ml) for 18 h. The expression of cell surface molecules, cytokine and chemokine levels in culture supernatants, cell viability, chemotaxis, and phagocytosis and killing of
Staphylococcus aureus
were measured.
Results
Maggot secretions dose-dependently inhibited production of the pro-inflammatory cytokines TNF-α, IL-12p40 and macrophage migration inhibitory factor by lipopolysaccharides- and lipoteichoic acid-stimulated monocytes, while enhancing production of the anti-inflammatory cytokine IL-10. Expression of cell surface receptors involved in pathogen recognition remained unaffected by secretions. In addition, maggot secretions altered the chemokine profile of monocytes by downregulating macrophage inflammatory protein-1β and upregulating monocyte chemoattractant protein-1 and IL-8. Nevertheless, chemotactic responses of monocytes were inhibited by secretions. Furthermore, maggot secretions did not affect phagocytosis and intracellular killing of
S. aureus
by human monocytes. Finally, secretions induced a transient rise in the intracellular cyclic AMP concentration in monocytes and Rp-cyclic AMPS inhibited the effects of secretions.
Conclusions/interpretation
Maggot secretions inhibit the pro-inflammatory responses of human monocytes through a cyclic AMP-dependent mechanism. Regulation of the inflammatory processes by maggots contributes to their beneficial effects on chronic wounds.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>19575178</pmid><doi>10.1007/s00125-009-1432-6</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-186X |
| ispartof | Diabetologia, 2009-09, Vol.52 (9), p.1962-1970 |
| issn | 0012-186X 1432-0428 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2723663 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Amputation Animals Biological and medical sciences Calliphoridae Cell Movement - drug effects Cell Movement - physiology Cell Survival - drug effects Cells, Cultured Chemokines Chemokines - secretion Chronic illnesses Cytokines Cytokines - secretion Diabetes Diabetes. Impaired glucose tolerance Diptera Endocrine pancreas. Apud cells (diseases) Endocrinopathies Etiopathogenesis. Screening. Investigations. Target tissue resistance Flow Cytometry Foot diseases Human Physiology Humans Inflammation - prevention & control Interleukin-10 - pharmacology Interleukin-10 - secretion Interleukin-10 - therapeutic use Internal Medicine Larva - drug effects Larva - physiology Leg ulcers Lipopolysaccharides - pharmacology Lucilia sericata Medical sciences Medicine Medicine & Public Health Metabolic Diseases Microorganisms Monocytes - cytology Monocytes - drug effects Monocytes - microbiology Monocytes - physiology Neutrophils Phagocytosis - drug effects Proteins Signal transduction Staphylococcus aureus Staphylococcus aureus - drug effects Teichoic Acids - pharmacology Wounds and Injuries - therapy |
| title | Maggot secretions suppress pro-inflammatory responses of human monocytes through elevation of cyclic AMP |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T16%3A17%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Maggot%20secretions%20suppress%20pro-inflammatory%20responses%20of%20human%20monocytes%20through%20elevation%20of%20cyclic%20AMP&rft.jtitle=Diabetologia&rft.au=van%20der%20Plas,%20M.%20J.%20A.&rft.date=2009-09-01&rft.volume=52&rft.issue=9&rft.spage=1962&rft.epage=1970&rft.pages=1962-1970&rft.issn=0012-186X&rft.eissn=1432-0428&rft_id=info:doi/10.1007/s00125-009-1432-6&rft_dat=%3Cproquest_pubme%3E20776119%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=213845764&rft_id=info:pmid/19575178&rfr_iscdi=true |