Two cap-binding proteins CBP20 and CBP80 are involved in processing primary microRNAs
MicroRNAs (miRNAs) are 21 nt RNAs that regulate many biological processes in plants by mediating translational inhibition or cleavage of target transcripts. Arabidopsis mutants defective in miRNA biogenesis have overlapping and highly pleiotropic phenotypes including serrated leaves and ABA hypersen...
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| Veröffentlicht in: | Plant and cell physiology 2008-11, Vol.49 (11), p.1634-1644 |
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| creator | Kim, S.(Rockefeller Univ., New York (USA)) Yang, J.Y Xu, J Jang, I.C Prigge, M.J Chua, N.H |
| description | MicroRNAs (miRNAs) are 21 nt RNAs that regulate many biological processes in plants by mediating translational inhibition or cleavage of target transcripts. Arabidopsis mutants defective in miRNA biogenesis have overlapping and highly pleiotropic phenotypes including serrated leaves and ABA hypersensitivity. Recent evidence indicates that miRNA genes are transcribed by RNA polymerase II (Pol II). Since Pol II transcripts are capped, we hypothesized that CBP (cap-binding protein) 20 and 80 may bind to capped primary miRNA (pri-miRNA) transcripts and play a role in their processing. Here, we show that cbp20 and cbp80 mutants have reduced miRNA levels and increased pri-miRNA levels. Co-immunoprecipitation experiments revealed that pri-miRNAs 159, 166, 168 and 172 could be associated with CBP20 and CBP80. We found that CBP20 and CBP80 are stabilized by ABA by a post-translational mechanism, and these proteins are needed for ABA induction of miR159 during seed germination. The lack of miR159 accumulation in ABA-treated seeds of cbp20/80 mutants leads to increased MYB33 and MYB101 transcript levels, and presumably higher levels of these positive regulators result in ABA hypersensitivity. Genetic and molecular analyses show that CBP20 and 80 have overlapping function in the same developmental pathway as SE and HYL1. Our results identify new components in miRNA biogenesis. |
| doi_str_mv | 10.1093/pcp/pcn146 |
| format | Article |
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Arabidopsis mutants defective in miRNA biogenesis have overlapping and highly pleiotropic phenotypes including serrated leaves and ABA hypersensitivity. Recent evidence indicates that miRNA genes are transcribed by RNA polymerase II (Pol II). Since Pol II transcripts are capped, we hypothesized that CBP (cap-binding protein) 20 and 80 may bind to capped primary miRNA (pri-miRNA) transcripts and play a role in their processing. Here, we show that cbp20 and cbp80 mutants have reduced miRNA levels and increased pri-miRNA levels. Co-immunoprecipitation experiments revealed that pri-miRNAs 159, 166, 168 and 172 could be associated with CBP20 and CBP80. We found that CBP20 and CBP80 are stabilized by ABA by a post-translational mechanism, and these proteins are needed for ABA induction of miR159 during seed germination. The lack of miR159 accumulation in ABA-treated seeds of cbp20/80 mutants leads to increased MYB33 and MYB101 transcript levels, and presumably higher levels of these positive regulators result in ABA hypersensitivity. Genetic and molecular analyses show that CBP20 and 80 have overlapping function in the same developmental pathway as SE and HYL1. Our results identify new components in miRNA biogenesis.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcn146</identifier><identifier>PMID: 18829588</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>ABA ; Abscisic Acid - metabolism ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; ARABIDOPSIS THALIANA ; ARN ; Cap-binding protein ; Cycloheximide - pharmacology ; EXPRESION GENICA ; EXPRESSION DES GENES ; FENOTIPOS ; GENE EXPRESSION ; Gene Expression Regulation, Plant ; Gene Silencing ; Genes, Plant ; Immunoprecipitation ; MicroRNA ; MicroRNAs - metabolism ; MUTANT ; MUTANTES ; MUTANTS ; Mutation ; PHENOTYPE ; PHENOTYPES ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Post-translational modification ; Primary microRNA ; Protein Binding ; PROTEINAS ; PROTEINE ; PROTEINS ; Rapid Papers ; RNA ; RNA Cap-Binding Proteins - genetics ; RNA Cap-Binding Proteins - metabolism ; RNA Processing, Post-Transcriptional - drug effects ; RNA, Plant - metabolism ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism ; TRANSCRIPCION ; TRANSCRIPTION</subject><ispartof>Plant and cell physiology, 2008-11, Vol.49 (11), p.1634-1644</ispartof><rights>The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org 2008</rights><rights>The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-27110ccca8d7e8e8722cd85075b0e49ea9d160dd99811637bc85b6ad1577099e3</citedby><cites>FETCH-LOGICAL-c524t-27110ccca8d7e8e8722cd85075b0e49ea9d160dd99811637bc85b6ad1577099e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18829588$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, S.(Rockefeller Univ., New York (USA))</creatorcontrib><creatorcontrib>Yang, J.Y</creatorcontrib><creatorcontrib>Xu, J</creatorcontrib><creatorcontrib>Jang, I.C</creatorcontrib><creatorcontrib>Prigge, M.J</creatorcontrib><creatorcontrib>Chua, N.H</creatorcontrib><title>Two cap-binding proteins CBP20 and CBP80 are involved in processing primary microRNAs</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>MicroRNAs (miRNAs) are 21 nt RNAs that regulate many biological processes in plants by mediating translational inhibition or cleavage of target transcripts. Arabidopsis mutants defective in miRNA biogenesis have overlapping and highly pleiotropic phenotypes including serrated leaves and ABA hypersensitivity. Recent evidence indicates that miRNA genes are transcribed by RNA polymerase II (Pol II). Since Pol II transcripts are capped, we hypothesized that CBP (cap-binding protein) 20 and 80 may bind to capped primary miRNA (pri-miRNA) transcripts and play a role in their processing. Here, we show that cbp20 and cbp80 mutants have reduced miRNA levels and increased pri-miRNA levels. Co-immunoprecipitation experiments revealed that pri-miRNAs 159, 166, 168 and 172 could be associated with CBP20 and CBP80. We found that CBP20 and CBP80 are stabilized by ABA by a post-translational mechanism, and these proteins are needed for ABA induction of miR159 during seed germination. The lack of miR159 accumulation in ABA-treated seeds of cbp20/80 mutants leads to increased MYB33 and MYB101 transcript levels, and presumably higher levels of these positive regulators result in ABA hypersensitivity. Genetic and molecular analyses show that CBP20 and 80 have overlapping function in the same developmental pathway as SE and HYL1. Our results identify new components in miRNA biogenesis.</description><subject>ABA</subject><subject>Abscisic Acid - metabolism</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>ARABIDOPSIS THALIANA</subject><subject>ARN</subject><subject>Cap-binding protein</subject><subject>Cycloheximide - pharmacology</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FENOTIPOS</subject><subject>GENE EXPRESSION</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Silencing</subject><subject>Genes, Plant</subject><subject>Immunoprecipitation</subject><subject>MicroRNA</subject><subject>MicroRNAs - metabolism</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTANTS</subject><subject>Mutation</subject><subject>PHENOTYPE</subject><subject>PHENOTYPES</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Post-translational modification</subject><subject>Primary microRNA</subject><subject>Protein Binding</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>PROTEINS</subject><subject>Rapid Papers</subject><subject>RNA</subject><subject>RNA Cap-Binding Proteins - genetics</subject><subject>RNA Cap-Binding Proteins - metabolism</subject><subject>RNA Processing, Post-Transcriptional - drug effects</subject><subject>RNA, Plant - metabolism</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>TRANSCRIPCION</subject><subject>TRANSCRIPTION</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkkuPFCEUhYnROO3oxr2mYqILk1IeRQEbk7HjOI4dnzOJcUNooFtmqqGEqlb_vbdTnfGx0AXhJHwcLvdchO4S_IRgxZ72tocVSdNeQzPSCFIrzNl1NMOY0RoLSQ7QrVIuMAbN8E10QKSkiks5Q-dn31JlTV8vQ3Qhrqs-p8GHWKr583cUVya6nZKgsq9C3KZu6x2IHWh9KdOdsDH5R7UJNqcPb47KbXRjZbri7-z3Q3R-_OJsflIv3r58NT9a1JbTZqipIARba410wksvBaXWSY4FX2LfKG-UIy12TilJSMvE0kq-bI0jXAislGeH6Nnk24_LjXfWxyGbTu_r0ckE_edJDF_0Om01hacoa8Dg0d4gp6-jL4PehGJ915no01h0q4RgvGH_BYkCR6U4gA_-Ai_SmCN0QVMM4QAkAHo8QdCvUrJfXZVMsN5lqiFTPWUK8P3fP_kL3YcIwMMJSGP_b6N64kIZ_Pcr0uRL3QomuD759Fm_bz_S0-PXC62AvzfxK5O0WedQ9CnMBFYwV1Jw9hPw2MEO</recordid><startdate>20081101</startdate><enddate>20081101</enddate><creator>Kim, S.(Rockefeller Univ., New York (USA))</creator><creator>Yang, J.Y</creator><creator>Xu, J</creator><creator>Jang, I.C</creator><creator>Prigge, M.J</creator><creator>Chua, N.H</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20081101</creationdate><title>Two cap-binding proteins CBP20 and CBP80 are involved in processing primary microRNAs</title><author>Kim, S.(Rockefeller Univ., New York (USA)) ; Yang, J.Y ; Xu, J ; Jang, I.C ; Prigge, M.J ; Chua, N.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-27110ccca8d7e8e8722cd85075b0e49ea9d160dd99811637bc85b6ad1577099e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>ABA</topic><topic>Abscisic Acid - metabolism</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>ARABIDOPSIS THALIANA</topic><topic>ARN</topic><topic>Cap-binding protein</topic><topic>Cycloheximide - pharmacology</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FENOTIPOS</topic><topic>GENE EXPRESSION</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Silencing</topic><topic>Genes, Plant</topic><topic>Immunoprecipitation</topic><topic>MicroRNA</topic><topic>MicroRNAs - metabolism</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>MUTANTS</topic><topic>Mutation</topic><topic>PHENOTYPE</topic><topic>PHENOTYPES</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Post-translational modification</topic><topic>Primary microRNA</topic><topic>Protein Binding</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>PROTEINS</topic><topic>Rapid Papers</topic><topic>RNA</topic><topic>RNA Cap-Binding Proteins - genetics</topic><topic>RNA Cap-Binding Proteins - metabolism</topic><topic>RNA Processing, Post-Transcriptional - drug effects</topic><topic>RNA, Plant - metabolism</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>TRANSCRIPCION</topic><topic>TRANSCRIPTION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, S.(Rockefeller Univ., New York (USA))</creatorcontrib><creatorcontrib>Yang, J.Y</creatorcontrib><creatorcontrib>Xu, J</creatorcontrib><creatorcontrib>Jang, I.C</creatorcontrib><creatorcontrib>Prigge, M.J</creatorcontrib><creatorcontrib>Chua, N.H</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, S.(Rockefeller Univ., New York (USA))</au><au>Yang, J.Y</au><au>Xu, J</au><au>Jang, I.C</au><au>Prigge, M.J</au><au>Chua, N.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two cap-binding proteins CBP20 and CBP80 are involved in processing primary microRNAs</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2008-11-01</date><risdate>2008</risdate><volume>49</volume><issue>11</issue><spage>1634</spage><epage>1644</epage><pages>1634-1644</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>MicroRNAs (miRNAs) are 21 nt RNAs that regulate many biological processes in plants by mediating translational inhibition or cleavage of target transcripts. Arabidopsis mutants defective in miRNA biogenesis have overlapping and highly pleiotropic phenotypes including serrated leaves and ABA hypersensitivity. Recent evidence indicates that miRNA genes are transcribed by RNA polymerase II (Pol II). Since Pol II transcripts are capped, we hypothesized that CBP (cap-binding protein) 20 and 80 may bind to capped primary miRNA (pri-miRNA) transcripts and play a role in their processing. Here, we show that cbp20 and cbp80 mutants have reduced miRNA levels and increased pri-miRNA levels. Co-immunoprecipitation experiments revealed that pri-miRNAs 159, 166, 168 and 172 could be associated with CBP20 and CBP80. We found that CBP20 and CBP80 are stabilized by ABA by a post-translational mechanism, and these proteins are needed for ABA induction of miR159 during seed germination. The lack of miR159 accumulation in ABA-treated seeds of cbp20/80 mutants leads to increased MYB33 and MYB101 transcript levels, and presumably higher levels of these positive regulators result in ABA hypersensitivity. Genetic and molecular analyses show that CBP20 and 80 have overlapping function in the same developmental pathway as SE and HYL1. Our results identify new components in miRNA biogenesis.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>18829588</pmid><doi>10.1093/pcp/pcn146</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | ABA Abscisic Acid - metabolism Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism ARABIDOPSIS THALIANA ARN Cap-binding protein Cycloheximide - pharmacology EXPRESION GENICA EXPRESSION DES GENES FENOTIPOS GENE EXPRESSION Gene Expression Regulation, Plant Gene Silencing Genes, Plant Immunoprecipitation MicroRNA MicroRNAs - metabolism MUTANT MUTANTES MUTANTS Mutation PHENOTYPE PHENOTYPES Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Post-translational modification Primary microRNA Protein Binding PROTEINAS PROTEINE PROTEINS Rapid Papers RNA RNA Cap-Binding Proteins - genetics RNA Cap-Binding Proteins - metabolism RNA Processing, Post-Transcriptional - drug effects RNA, Plant - metabolism RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism TRANSCRIPCION TRANSCRIPTION |
| title | Two cap-binding proteins CBP20 and CBP80 are involved in processing primary microRNAs |
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