Purification, crystallization and preliminary X-ray crystallographic studies of the complex between Smc5 and the SUMO E3 ligase Mms21

Smc5/6, a protein complex that belongs to the structural maintenance of chromosome (SMC) family, plays a key role in DNA replication, sister chromatid recombination and DNA damage repair. The complex contains eight subunits, including a SUMO E3 ligase Mms21 (Nse2). The activity of Mms21 is important...

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Veröffentlicht in:	Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2009-08, Vol.65 (8), p.849-852
Hauptverfasser:	Duan, Xinyuan, Ye, Hong
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence BASIC BIOLOGICAL SCIENCES Cell Cycle Proteins - chemistry Cell Cycle Proteins - genetics CHROMATIDS CHROMOSOMES Cloning, Molecular CRYSTALLIZATION Crystallization Communications Crystallography, X-Ray DIFFRACTION DNA damage repair DNA DAMAGES DNA Primers DNA REPLICATION ESCHERICHIA COLI GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE LIGASES MAINTENANCE Mms21 Protein Conformation PROTEINS PURIFICATION RECOMBINATION REGULATIONS REPAIR Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - genetics SMC proteins Smc5/6 complex SPACE GROUPS SUMO SUMO-1 Protein - chemistry SUMO-1 Protein - genetics X-RAY DIFFRACTION
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