Characterization of Engineered Channelrhodopsin Variants with Improved Properties and Kinetics

Channelrhodopsin 2 (ChR2), a light-activated nonselective cationic channel from Chlamydomonas reinhardtii, has become a useful tool to excite neurons into which it is transfected. The other ChR from Chlamydomonas, ChR1, has attracted less attention because of its proton-selective permeability. By ma...

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Veröffentlicht in:	Biophysical journal 2009-03, Vol.96 (5), p.1803-1814
Hauptverfasser:	Lin, John Y., Lin, Michael Z., Steinbach, Paul, Tsien, Roger Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Algal Proteins - chemistry Algal Proteins - metabolism Analysis of Variance Animals Bacteria Cationic Cell Line Cells, Cultured Channels Channels, Receptors, and Electrical Signaling Chlamydomonas reinhardtii Electric Stimulation Hippocampus - physiology Humans Inactivation Least-Squares Analysis Membrane Potentials Membranes Microscopy, Confocal Mutation Neurons Neurons - physiology Patch-Clamp Techniques Photic Stimulation Protein Engineering Rats Reaction kinetics Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - metabolism Rhodopsin - chemistry Rhodopsin - genetics Rhodopsin - metabolism Spectra Stimulation Trains
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